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Rotor-Gene® Q (Qiagen) Real Time PCR Detection System

Laboratory Name: Biochemistry & Molecular Biology Specialized Laboratory Lab Supervisor: Dr. Noura Abou Zeinab Location: Laboratory Building (A3), GF, Lab: G104-105, Debbieh Campus Tel. Ext.: 3363

The Rotor-Gene®Q instrument (SN: R121240)

with its Software (Version 2.3.1.49) is designed to

perform real-time and end-point thermal cycling

using the polymerase chain reaction (PCR) and

high-resolution melting analysis (HRM) in

molecular biology applications.

36-Well Rotor and 72-Well Rotor are available.

with the instrument. 36-Well Rotor Locking Ring

enables the use of 0.2 ml tubes. The 72-Well

Rotor and Locking Ring are used with Strip Tubes

and Caps, 0.1 ml, which can be used for volumes

as low as 20 µl. The caps provide a safe and

reliable seal.

Applications: It is highly suited to precisely

quantitate and analyze changes in gene

expression, genotyping, pathogen detection,

concentration measurement, protein analysis,

enzyme kinetics, and many other areas of

research. The powerful and user-friendly software

provides simplicity for beginners as well as an

open experimental platform for advanced users. The 36-Well Rotor. The 72-Well Rotor.

Applications for all thermal cyclers availabe (PX2, Q-Cycler, BIORAD)

PCR is widely used as a diagnostic and research tool. Its applications are widespread over many disciplines,

including molecular biology, microbiology, genetics, clinical diagnostics, forensic science, environmental science,

hereditary studies and paternity testing.

Copying and amplifying fragments of nucleic acids (DNA).

Analyzing clinical specimens for the presence of infectious agents, e.g. HIV, hepatitis, human papillomavirus

and others.

In forensic science, using PCR-based DNA fingerprinting, fragments of DNA can be isolated from a crime scene

and compared to a database of DNA of convicts or criminals.

DNA fingerprinting is also used in paternity testing.

Analysis of genetic mutations that occurs in cancer and many genetic diseases.

Assisting the DNA sequencing technique where segments of DNA from an area of interest can be easily

amplified to study their sequences.

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C1000TM Thermal Cycler PCR BioRAD

PCR Thermocycler Quanta Biotech Ltd (Q-Cycler)

The C1000 cycler (184-1000; SN: cc008327) cycler.

Dual 48/48 Reaction Module — It’s a Two Thermal

Cyclers in One. It offers a gradient-enabled dual

reaction module allowing to run two independent

experiments at the same time. It is convenient for

protocol optimization, allowing more variables to be

tested simultaneously

The Q-Cycler (7013000; SN: CM5057).

The Q-Cycler II independently controls and displays a real

time data for both itself and each connected satellite.

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Thermo- Px2 Thermal Cycler (Thermo Electron Corporation)

Thermo Electron’s Px2 Thermal Cycler – reliable PCR

performance (SN: Px220328; Numelab Company) Simple to

use, technologically advanced solution for thermal cycling

with: Accurate temperature control for optimal PCR

performance; Gradient capability for rapid protocol

optimization; Outstanding well to well uniformity for

reproducible PCR performance; High definition 1/4 VGA

display for enhanced user-friendliness; Keypad with

functional soft keys for easy software navigation

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Thermo Lbsystems Multiskan® EX - ELISA microplate reader

Thermo Lbsystems Wellwash® - ELISA washer

The Thermo Scientic Multiskan EX

(SN: 355-1-10183) is a basic reader

for endpoint and kinetic assays.

Additionally, the Multisikan EX with

onboard software (Ascent Software)

offers a wide range of data

reductions and report generations.

Absorbance Filters available: 405nm,

450nm, 540nm, and 620nm.

Applications: For reading 96-well

microplates, adapted ELISA and

other colorimetric assays and

immunological reactions.

Other applications include: Protein assays; Growth curve and hormone assays; Endotoxins; Food

diagnostics; HIV assays; Hybridization assays; Minisequencing assays; Cytotoxicity; Cell adhesion; Signal

transduction; Endotoxins; Antioxidants; Food diagnostics.

The Wellwash (SN: 006-1-10144) is a semiautomatic

microplate washer (96-well plates), comprising pump

and washer units. The instrument is supplied with

both 8- and 12-way interchangeable wash heads.

Applications: It can be used in research or routine-

test laboratories to wash microtiter microplates and

strips in 96-well plate format that meet the SBS

standards.

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GelDoc-It®2 & ChemiDoc-It®

2 Imagers

The GelDoc-It2 Imager is a high resolution imager,

capable of capturing, documenting and analyzing

fluorescent gel images, chemiluminescent blot

images, including Western blots. The system is

equipped with a cooled, scientific-grade CCD

camera.

Image incorporate a light-tight darkroom with

VisionWorks® software for image acquisition and

analysis functions. The full package VisionWorks™

L.S. Image acquisition and Analysis Software (SN:

478457-8246UL-34DDT2-4906zz) is included for

use on an external computer. Analysis functions

include: band analysis, histograms, lane profile

graphs, total area density, Western blot

densitometry and data reporting.

The darkroom has a UV-blocking gel viewer

window, built-in overhead epi white lighting, a UV

transilluminator and a four-position emission

filter wheel with an ethidium bromide (EtBr)

emission filter included as standard.

Applications: capturing, documenting and analyzing fluorescent gel images, as well chemiluminescent blot

images, including Western blots.

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HL-2000 HybriLinker Hybridization Oven

Ultracentrifuge (Beckman Coulter; OptimaL-100xP)

Innovative HL-2000 HybriLinkerTM System (SN: 053003-002; CA

91786) is designed with the combination benefits of a

Hybridization Oven and UV Crosslinker (254nm UV) in one self-

contained, space saving unit for crosslinking prior to

hybridization protocols.

The hybridization oven: is a cost-effective, high-performance,

10-bottle hybridization oven with microprocessor temperature

and variable speed controls. The Hybridizer can be set to

operate anywhere from 10 to 15 RPM. A touch-sensitive keypad

and microprocessor provide accurate temperature control from

ambient 10°C to 99.9°C. Two different hybridizer bottle sizes are

available for use within the hybridizer using the standard

carousel provided with the unit.

The crosslinker chamber: has shortwave 254nm, 8watt,

ultraviolet tubes supply uniform overhead UV illumination;

Preset and manual controls for ultraviolet or time exposures;

Preset exposure delivers 120,000 microjoules or five minutes of

exposure; Microprocessor measures and controls UV output.

Applications: Used for crosslinking of nucleic acids (DNA or RNA) to a nitrocellulose membrane, for hybridization

reactions in Southern and northern blotting assays, other UV Crosslinking.

Beckman Coulter; OptimaL-100xP ultracentrifuge

(SN: SERLxpo5G09; CA 94304) is capable of

generating maximum g- force 802,400xg (with a

maximum speeds up to 100,000 rpm0. Beckman

Coulter’s Ultracentrifuge enables researchers to

perform more separations in less time.

Applications: For proteomics, cytomics or

genomics, centrifugation of solutions containing:

RNA, DNA, microsomal p450 extraction and more.

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Cold Centrifuge-Centurion model K2015R – Bench top

Cold centrifuge Hero lab GmbH (UniCen 15DR)

Cold Centrifuge-Centurion model K2015R – Bench

top (SN: 217296-1): It has two rotors: BRK 5324 rotor

(capacity 24 x 2ml; For separation of solutions and

biological samples in a 1.5 ml tubes at 500-15000 rpm

at 40C - 250C) and BRK 5424 rotor (capacity 24 x 15 ml;

For separation of solutions and biological samples in

15 ml tubes at 500-6000 rpm at 40C - 250C).

HeroLab" Universal (Refrigerated) Benchtop Centrifuges

UniCen™ 15DR (SN: 0303715100): It is provided with two

rotors that can separate solutions and biological samples in

1.5-2 ml or 10 ml tubes at 5000 up to15300 rpm / 21,460 xg.

Temperature (Range from -10 to +40℃).

Applications of cold centrifuges: It is used to purify cells, subcellular organelles, viruses, proteins, and nucleic

acids (DNA and RNA).

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Thermo Scientific Heraeus Pico 17 Microcentrifuges

The Heraeus Pico17 (SN: 40651137) for routine laboratory work.

The 24-place rotor can be used for all 1.5 to 2.0 ml tubes, including

mini-preps and spin columns. It has a Max RCF 17,000 x g (Max

Speed 13,300 rpm); Process samples faster with unmatched

acceleration and deceleration rates.

Applications: For separation of solutions and biological samples. It

is used to purify cells, subcellular organelles, viruses, proteins, and

DNA.

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UV Spectrophotometer- UV/VIS (EvolutionTM 60,Thermo Scientific)

Spectro UV-2505 UV-Vis Spectrophotometer (LABOMED Inc)

The Evolution™ 60 (SN:2Q4M239002; CAT 840-189900) is a UV-Visible spectrophotometer with 6-position cell changer. The VISIONlite™ PC software provided offer intuitive method setup, data reporting, and advanced calculation capabilities. Biochemical tests are built into the Local Control software, and PC control analyzer packages are available for enzymatic food analysis, color calculations and materials calculations. The VISIONlite, consists of applications for wavelength scanning, fixed wavelength analysis, quantitative analysis and kinetics. Accessories provided: Rectangular Thermostatted single cell holder (Thermo Block; 840-206700 ;Thomas 1189N83) that is used to control the temperature of one 10 mm pathlength square cuvette within the range of the recirculator.

Applications: To determine the absorbance in solutions with wavelength range: 190 -1100nm in 1.5 ml to a

maximum 4 ml cuvettes; Protein/nucleic acid measurement; Kinetics measurement/analysis; Quantitative protein

analysis program with six different calibration methods.

Spectro UV-2505. Model UV-2505 works

in the ultraviolet and visible range of

195-1050nm and has a 4nm bandwidth.

Applications: Spectrophotometer Model

UV-2505 can be used extensively for

pharmaceutical manufacturing, health,

clinical laboratory, biochemistry,

petrochemistry, environmental

protection, quality control, water

management, food processing,

agriculture, and for a wide range of

businesses and industries.

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WiseTis – Homogenizer Wise Tis HG-15D

BANDELIN SONOPULS ULTRASONIC HD 2070 homogeniser

SONOPULS HD 2070 homogenizers

(SN:599.00045988.005) for use in laboratories

(For volumes from 1 ml to 200 ml). Ultrasonic

homogenizers in 2 sizes with 20 kHz

homogeneous sound and variable duration of

sonication, power output, and pulsing. With

integrated amplitude control 10-100 % and

actual value display, pulsation, AMPLICHRON®-

System, built-in timer, remote controlled.

Ready-to-operate basic equipment for volumes

from 2 ml to 50 ml

Applications: Emulsification; homogenizing of

substances; suspending; cell disruption

(bacteria, virus, tissues); degassing of fluids;

accelerating chemical reactions; sonochemistry;

sample preparation for HPLC; sample

preparation for particle size analysis; waste-

water analysis.

The digital laboratory device WiseTis HG-15D

(SN: HG15D081117001; set with WiseTis HG-15D,

dispersing tool HT1018, stand rodST110 and clamp CL200)

is built especially for exact homogenizing and is equipped

with high quality electronic controller.

Applications: Disruption of cells and tissues.

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SONIC RUPTUR 250 (OMNI INTERNATIONAL; The Homogenizer Compound)

The Omni Ruptor 250 Watt Ultrasonic Cell

Disrupter (SN: Z1876) is designed to handle a

variety of applications. Sample volumes can be

used with a range from 0.25 mL to 1000 mL

with the availability of different size solid

titanium tips. Pulse mode for sensitive samples;

0-15 minute adjustable timer; Auto-tuning for

convenience of use and optimal processing

efficiency; Power-emitted display for accuracy

and repeatability.

Applications: including disruption of cells,

bacteria, spores, emulsification of immiscible

liquids, homogenization, uniform dispersion of

particles in liquids, acceleration of reactions,

depolymerization of large molecules

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MUPID-exU Horizontal Electrophoresis System

Western Blotters (Vertical Gel Electrophoresis Unit)

OmniPAGE TETRAD Mini-Protein Electrophoresis

The MUPID-exU (SN:71295) is a submarine electrophoresis

system and includes a power supply, direct visible gel box with

high and complete UV-transmittancy. Multisample

analysis (support for multipipettors) multichannel compatible

gel loading combs (Allows simultaneous electrophoresis of 104

samples with a single gel), gel pouring stand, and a built in timer

(with alarm function) for set runs or continuous run modes (The

timer can be set within the range of 1-99 minutes). Variation of

input and output voltages: Input voltage supports 100-240V.

Output voltage can be selected from 7 levels.

Applications: For electrophoretic separation of DNA fragments and RNA molecules on agarose gels: Used in forensic medicine, molecular biology, genetics, biochemistry, microscopical biology; and Used in cloning, RFLP, PCR, and other molecular biology assays

OmniPAGE TETRAD Mini-Protein

Electrophoresis Package (SN: VS09072445)

for 1mm self-cast gels, Includes: CVS10DSYS-

CU– 10x10cm mini-vertical unit with casting

base and external casting stand, plus 2x1mm

12-sample combs, 2x plain glass plates with

1mm spacer and 2x notched plates.

Applications: For electrophoretic separation

of protein samples and for blotting proteins

on nitrocellulose, or PVDF membranes in

western blotting assays.

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Tissue Processor - HistoMaster 2052//2L/Z/A analog, MDS

Manual Rotary Microtome - microTec (Cut 4050)

Histomaster 2052/2L/Z/A analog system (SN: RW-

0909060) Download up to 380 cassettes; Easy

operation and maintenance; Possibility of

completing Rotary device for ease of maintenance;

With digital control.

The system possesses 12 stations, three paraffin

baths, and two metallic baskets with a maximum

loading up to 200 cartridges.

Applications: To prepare histological blocks from

different biological samples.

MicroTec CUT 4050 (SN: 500-410) Manual Rotary

Microtome with Oil, Dustcover & Tool Kit,

Including Knife Holder-Basic Unit, Disposable

Blade Holder, Clamping Plate-Low Profile,

Adapter-Orientable, Clamping Lever Left,

Universal Cassette Clamp and Section Waste Tray.

Applications: To cut thin slices of histological

blocks. Cuts range: starting from 1μm - 60μm

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Digital Cryo console Cold Plate Model ES 500–2

Paraffin Wax Dispenser (J.P.SELECTA Dispenser)

Cryo console (SN: CJHS0102ES500-2C-006) is one

component of the integrated embedding center.

The Cryo Console consists an advanced compressor

that can cool the paraffin blocks rapidly. The Teflon

coated surface guarantee a minimum of ice

formation and easy clean. Working temperature:

from 15℃ to -15℃, increments 1℃;Capacity: 70

cassettes

Applications: The Cryo console is used to chill the

paraffin blocks rapidly and to ensure the best

stability cooling performance. it can be used as a

standalone unit near microtome station and

embedding unit. It can be used with 60 cassettes.

Paraffin wax dispenser (SN: 0531589). Internal tank

made of aluminium coated with black indelible PTFE.

Independently heated tap.

Adjustable temperature from ambient +5 TO 100℃.

Stability ±1.5℃.

Applications: To dispense paraffin wax for the

preparation of different histological blocks with

temperatures of 00C -1000C.

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Thermostatic Baths: Paraffin wax floating out bath for histology

samples “Termofin” (J.P.SELECTA, Thermofen)

Forma (-860C)- Ultra-Low Temperature Upright Freezers (Thermo)

Paraffin wax floating out bath (SN:

0531716): it is a Bath and lid made of black

PTFE.

Adjustable temperature from 40°C TO

80°C. Stability ±1 °C.

Applications: Suitable for the fusion and

preservation of paraffin wax in a liquid

state that enables the users to see the

floated sections.To place slices of the tissue

embedded paraffin ribbons in water bath

(00C -1000C).

Thermo Scientific Forma freezers (SN:804603).

Applications: To store specimens, tissues, and reagents at ~

-700C.

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Hot Drying Oven (CONTHERM)

The Contherm Thermotec Designer Series Ovens (CAT 8050-8150, SN: 07561, 50L capacity; and SN: 08306,

100 L capacity).

Applications: To dry samples, glasswares, and to melt wax. Temperature range: 200C -1000C

The Contherm Thermotec Designer Series Ovens

(CAT 8050-8150, SN: 07561, 50L capacity)

The Contherm Thermotec Designer Series Ovens

(CAT 8050-8150, SN: 08306, 100 L capacity)

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Laboratory Name: Central Laboratory Of Biochemistry & Molecular Biology Lab Laboratory Name: Central Laboratory Of Biochemistry & Molecular Tel. Ext.: 3363 Tel. Ext.: 336

Steam Sterilizer

Incubator

Laboratory Name: Specialized Microbiology Laboratory Lab Supervisor: Mrs. Lama Fayad Location: Laboratory Building (A3), GF, Lab: G110, Debbieh Campus Tel. Ext.: 3365

Steam Sterilizer (Raypa, 36615), is used to sterilize buffers, solutions, culture media, glassware, surgical instruments, plastic wares, equipments, medical and pathogenic wastes by subjecting them to high-pressure saturated steam at 121 °C for around 15–20 minutes. Applications Steam Sterilizer is a simple yet very effective decontamination method; it is widely used in microbiology, medicine, dentistry and other fields.

Incubator (Memmert & Wtc binder, E306.0152 & 920378) is a device used to grow and maintain microbiological cultures or cell cultures by maintaining optimal temperature, humidity and gaseous content of the atmosphere inside. Applications Incubators are essential for a lot of experimental work in cell biology, microbiology and molecular biology.

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Shaking Incubator

Cooling Incubator

Shaking Incubator (ZHWY SERIES DOUBLE- LAYER CONSTANT TEMPERATURE) can be used for growth of bacterial and fungal cultures at different rpm (from 30 to 300 rpm) and temperatures (from 4°C to 60°C). Applications It has a wide range of application in research of medicine, biology, pharmacy and environmental research. It is used for cell culturing, cell aeration, solubility studies and ELISA incubations.

Cooling Incubator (STIK, 012080027) is intended for environmental test, thermostat test, low temperature preservation. It is used for applications that require cold temperatures, in the range of room temperature and below down to sub-zero temperatures (-10°C). Applications It is widely used in research, drug testing, agricultural research, and environmental protection fields.

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Centrifuge

Nitrogen Distiller

Centrifuge (CENTURION, 13762-1) is used for routine

laboratory work. It has a Max RCF 13500 g; max speed

12000 rpm; various acceleration and deceleration rates.

Applications

Used for separation of solutions and biological samples.

This apparatus is found in most laboratories of academic

and clinical researches. It is used to purify cells,

subcellular organelles, viruses, proteins and DNA.

Nitrogen Distiller (RAYPA, DNP- 2000) The distillation unit is used to perform nitrogen analysis and protein determination according to Kjeldahl method, in cells, food industry and other applications in environmental and chemical industries after having digested the sample accurately.

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Fermenter

Vacuum Freeze Dryer

Fermenter (BIOTRON, GR-1002F-06) is used for the cultivation of microorganisms that produce hormones, vaccines, antibiotics, vitamins, enzymes, and other metabolites. It provides a controlled environment for optimal biomass/product yields. It is used for large cultures containing 3 liters of growth medium. Applications Fermenters are made for research and production in biotechnology institutes, universities, biochemical industries, food, agricultural, pharmaceutical industries, and life science industries. They maintain optimal conditions for the growth of microorganisms.

Vacuum Freeze Dryer (BOYIKANG, FD-ID-50) is a dehydration technique, which enables liquid or slurry products, which have previously been frozen to be dried under a vacuum. Of the various methods of dehydration, freeze drying is especially suited for substances that are heat sensitive.

Applications

Vacuum Freeze Dryer is used for preservation of temperature sensitive products, such as enzymes, blood plasma, vaccines, etc, and for conversion of suspension and liquid samples to powder at -200°C. It is used to improve storage life and improve marketing of the end product. Also, vacuum Freeze Dryer is an important process in sample preparation and for the preservation and storage of biological, pharmaceuticals and foods, it has been extensively used in the development of pharmaceuticals (e.g., antibiotics) and preservation of biological substances (e.g., proteins, plasma, viruses and cell lines).

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Computerized Spectrophotometer

Natural Convection Oven

Colony Counter

Computerized Spectrophotometer (JASCO

RESEARCH GRADE V-630 UV/VIS, C284961148) works

in the ultraviolet and visible range of 190-1100 nm.

Applications

It can be used extensively for pharmaceutical

manufacturing, health, clinical laboratory,

biochemistry, petro chemistry, environmental

protection, quality control, water management, food

processing, agriculture, and for a wide range of

businesses and industries.

Natural Convection Oven (SHINSAENG SDON-301, TIG22-SDON301-01) is a hydraulic thermostat provides reliable temperature control with a range from 5°C above ambient to 210°C. Applications It is used for sterilization, drying, and thermal storage tasks that do not require high drying rates.

Colony counter (J-2 Colony counter semi automatic digital) is a device used for counting colonies of bacteria growing in a culture and to calculate the bacterial concentration in samples. It is used also to evaluate the quality of food and water.