Rotor-Gene® Q (Qiagen) Real Time PCR Detection Files/Science/Biology... · Rotor-Gene® Q (Qiagen)

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Rotor-Gene Q (Qiagen) Real Time PCR Detection System

Laboratory Name: Biochemistry & Molecular Biology Specialized Laboratory Lab Supervisor: Dr. Noura Abou Zeinab Location: Laboratory Building (A3), GF, Lab: G104-105, Debbieh Campus Tel. Ext.: 3363

The Rotor-GeneQ instrument (SN: R121240)

with its Software (Version 2.3.1.49) is designed to

perform real-time and end-point thermal cycling

using the polymerase chain reaction (PCR) and

high-resolution melting analysis (HRM) in

molecular biology applications.

36-Well Rotor and 72-Well Rotor are available.

with the instrument. 36-Well Rotor Locking Ring

enables the use of 0.2 ml tubes. The 72-Well

Rotor and Locking Ring are used with Strip Tubes

and Caps, 0.1 ml, which can be used for volumes

as low as 20 l. The caps provide a safe and

reliable seal.

Applications: It is highly suited to precisely

quantitate and analyze changes in gene

expression, genotyping, pathogen detection,

concentration measurement, protein analysis,

enzyme kinetics, and many other areas of

research. The powerful and user-friendly software

provides simplicity for beginners as well as an

open experimental platform for advanced users. The 36-Well Rotor. The 72-Well Rotor.

Applications for all thermal cyclers availabe (PX2, Q-Cycler, BIORAD)

PCR is widely used as a diagnostic and research tool. Its applications are widespread over many disciplines,

including molecular biology, microbiology, genetics, clinical diagnostics, forensic science, environmental science,

hereditary studies and paternity testing.

Copying and amplifying fragments of nucleic acids (DNA).

Analyzing clinical specimens for the presence of infectious agents, e.g. HIV, hepatitis, human papillomavirus

and others.

In forensic science, using PCR-based DNA fingerprinting, fragments of DNA can be isolated from a crime scene

and compared to a database of DNA of convicts or criminals.

DNA fingerprinting is also used in paternity testing.

Analysis of genetic mutations that occurs in cancer and many genetic diseases.

Assisting the DNA sequencing technique where segments of DNA from an area of interest can be easily

amplified to study their sequences.

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C1000TM Thermal Cycler PCR BioRAD

PCR Thermocycler Quanta Biotech Ltd (Q-Cycler)

The C1000 cycler (184-1000; SN: cc008327) cycler.

Dual 48/48 Reaction Module Its a Two Thermal

Cyclers in One. It offers a gradient-enabled dual

reaction module allowing to run two independent

experiments at the same time. It is convenient for

protocol optimization, allowing more variables to be

tested simultaneously

The Q-Cycler (7013000; SN: CM5057).

The Q-Cycler II independently controls and displays a real

time data for both itself and each connected satellite.

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Thermo- Px2 Thermal Cycler (Thermo Electron Corporation)

Thermo Electrons Px2 Thermal Cycler reliable PCR performance (SN: Px220328; Numelab Company) Simple to

use, technologically advanced solution for thermal cycling

with: Accurate temperature control for optimal PCR

performance; Gradient capability for rapid protocol

optimization; Outstanding well to well uniformity for

reproducible PCR performance; High definition 1/4 VGA

display for enhanced user-friendliness; Keypad with

functional soft keys for easy software navigation

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Thermo Lbsystems Multiskan EX - ELISA microplate reader

Thermo Lbsystems Wellwash - ELISA washer

The Thermo Scientic Multiskan EX

(SN: 355-1-10183) is a basic reader

for endpoint and kinetic assays.

Additionally, the Multisikan EX with

onboard software (Ascent Software)

offers a wide range of data

reductions and report generations.

Absorbance Filters available: 405nm,

450nm, 540nm, and 620nm.

Applications: For reading 96-well

microplates, adapted ELISA and

other colorimetric assays and

immunological reactions.

Other applications include: Protein assays; Growth curve and hormone assays; Endotoxins; Food

diagnostics; HIV assays; Hybridization assays; Minisequencing assays; Cytotoxicity; Cell adhesion; Signal

transduction; Endotoxins; Antioxidants; Food diagnostics.

The Wellwash (SN: 006-1-10144) is a semiautomatic

microplate washer (96-well plates), comprising pump

and washer units. The instrument is supplied with

both 8- and 12-way interchangeable wash heads.

Applications: It can be used in research or routine-

test laboratories to wash microtiter microplates and

strips in 96-well plate format that meet the SBS

standards.

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GelDoc-It2 & ChemiDoc-It

2 Imagers

The GelDoc-It2 Imager is a high resolution imager,

capable of capturing, documenting and analyzing

fluorescent gel images, chemiluminescent blot

images, including Western blots. The system is

equipped with a cooled, scientific-grade CCD

camera.

Image incorporate a light-tight darkroom with

VisionWorks software for image acquisition and

analysis functions. The full package VisionWorks

L.S. Image acquisition and Analysis Software (SN:

478457-8246UL-34DDT2-4906zz) is included for

use on an external computer. Analysis functions

include: band analysis, histograms, lane profile

graphs, total area density, Western blot

densitometry and data reporting.

The darkroom has a UV-blocking gel viewer

window, built-in overhead epi white lighting, a UV

transilluminator and a four-position emission

filter wheel with an ethidium bromide (EtBr)

emission filter included as standard.

Applications: capturing, documenting and analyzing fluorescent gel images, as well chemiluminescent blot

images, including Western blots.

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HL-2000 HybriLinker Hybridization Oven

Ultracentrifuge (Beckman Coulter; OptimaL-100xP)

Innovative HL-2000 HybriLinkerTM System (SN: 053003-002; CA

91786) is designed with the combination benefits of a

Hybridization Oven and UV Crosslinker (254nm UV) in one self-

contained, space saving unit for crosslinking prior to

hybridization protocols.

The hybridization oven: is a cost-effective, high-performance,

10-bottle hybridization oven with microprocessor temperature

and variable speed controls. The Hybridizer can be set to

operate anywhere from 10 to 15 RPM. A touch-sensitive keypad

and microprocessor provide accurate temperature control from

ambient 10C to 99.9C. Two different hybridizer bottle sizes are

available for use within the hybridizer using the standard

carousel provided with the unit.

The crosslinker chamber: has shortwave 254nm, 8watt,

ultraviolet tubes supply uniform overhead UV illumination;

Preset and manual controls for ultraviolet or time exposures;

Preset exposure delivers 120,000 microjoules or five minutes of

exposure; Microprocessor measures and controls UV output.

Applications: Used for crosslinking of nucleic acids (DNA or RNA) to a nitrocellulose membrane, for hybridization

reactions in Southern and northern blotting assays, other UV Crosslinking.

Beckman Coulter; OptimaL-100xP ultracentrifuge

(SN: SERLxpo5G09; CA 94304) is capable of

generating maximum g- force 802,400xg (with a

maximum speeds up to 100,000 rpm0. Beckman

Coulters Ultracentrifuge enables researchers to

perform more separations in less time.

Applications: For proteomics, cytomics or

genomics, centrifugation of solutions containing:

RNA, DNA, microsomal p450 extraction and more.

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Cold Centrifuge-Centurion model K2015R Bench top

Cold centrifuge Hero lab GmbH (UniCen 15DR)

Cold Centrifuge-Centurion model K2015R Bench

top (SN: 217296-1): It has two rotors: BRK 5324 rotor

(capacity 24 x 2ml; For separation of solutions and

biological samples in a 1.5 ml tubes at 500-15000 rpm

at 40C - 250C) and BRK 5424 rotor (capacity 24 x 15 ml;

For separation of solutions and biological samples in

15 ml tubes at 500-6000 rpm at 40C - 250C).

HeroLab" Universal (Refrigerated) Benchtop Centrifuges

UniCen 15DR (SN: 0303715100): It is provided with two

rotors that can separate solutions and biological samples in

1.5-2 ml or 10 ml tubes at 5000 up to15300 rpm / 21,460 xg.

Temperature (Range from -10 to +40).

Applications of cold centrifuges: It is used to purify cells, subcellular organelles, viruses, proteins, and nucleic

acids (DNA and RNA).

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Thermo Scientific Heraeus Pico 17 Microcentrifuges

The Heraeus Pico17 (SN: 40651137) for routine laboratory work.

The 24-place rotor can be used for all 1.5 to 2.0 ml tubes, including

mini-preps and spin columns. It has a Max RCF 17,000 x g (Max

Speed 13,300 rpm); Process samples faster with unmatched

acceleration and deceleration rates.

Applications: For separation of solutions and biological samp