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Rotor-Gene Q (Qiagen) Real Time PCR Detection System
Laboratory Name: Biochemistry & Molecular Biology Specialized Laboratory Lab Supervisor: Dr. Noura Abou Zeinab Location: Laboratory Building (A3), GF, Lab: G104-105, Debbieh Campus Tel. Ext.: 3363
The Rotor-GeneQ instrument (SN: R121240)
with its Software (Version 184.108.40.206) is designed to
perform real-time and end-point thermal cycling
using the polymerase chain reaction (PCR) and
high-resolution melting analysis (HRM) in
molecular biology applications.
36-Well Rotor and 72-Well Rotor are available.
with the instrument. 36-Well Rotor Locking Ring
enables the use of 0.2 ml tubes. The 72-Well
Rotor and Locking Ring are used with Strip Tubes
and Caps, 0.1 ml, which can be used for volumes
as low as 20 l. The caps provide a safe and
Applications: It is highly suited to precisely
quantitate and analyze changes in gene
expression, genotyping, pathogen detection,
concentration measurement, protein analysis,
enzyme kinetics, and many other areas of
research. The powerful and user-friendly software
provides simplicity for beginners as well as an
open experimental platform for advanced users. The 36-Well Rotor. The 72-Well Rotor.
Applications for all thermal cyclers availabe (PX2, Q-Cycler, BIORAD)
PCR is widely used as a diagnostic and research tool. Its applications are widespread over many disciplines,
including molecular biology, microbiology, genetics, clinical diagnostics, forensic science, environmental science,
hereditary studies and paternity testing.
Copying and amplifying fragments of nucleic acids (DNA).
Analyzing clinical specimens for the presence of infectious agents, e.g. HIV, hepatitis, human papillomavirus
In forensic science, using PCR-based DNA fingerprinting, fragments of DNA can be isolated from a crime scene
and compared to a database of DNA of convicts or criminals.
DNA fingerprinting is also used in paternity testing.
Analysis of genetic mutations that occurs in cancer and many genetic diseases.
Assisting the DNA sequencing technique where segments of DNA from an area of interest can be easily
amplified to study their sequences.
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C1000TM Thermal Cycler PCR BioRAD
PCR Thermocycler Quanta Biotech Ltd (Q-Cycler)
The C1000 cycler (184-1000; SN: cc008327) cycler.
Dual 48/48 Reaction Module Its a Two Thermal
Cyclers in One. It offers a gradient-enabled dual
reaction module allowing to run two independent
experiments at the same time. It is convenient for
protocol optimization, allowing more variables to be
The Q-Cycler (7013000; SN: CM5057).
The Q-Cycler II independently controls and displays a real
time data for both itself and each connected satellite.
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Thermo- Px2 Thermal Cycler (Thermo Electron Corporation)
Thermo Electrons Px2 Thermal Cycler reliable PCR performance (SN: Px220328; Numelab Company) Simple to
use, technologically advanced solution for thermal cycling
with: Accurate temperature control for optimal PCR
performance; Gradient capability for rapid protocol
optimization; Outstanding well to well uniformity for
reproducible PCR performance; High definition 1/4 VGA
display for enhanced user-friendliness; Keypad with
functional soft keys for easy software navigation
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Thermo Lbsystems Multiskan EX - ELISA microplate reader
Thermo Lbsystems Wellwash - ELISA washer
The Thermo Scientic Multiskan EX
(SN: 355-1-10183) is a basic reader
for endpoint and kinetic assays.
Additionally, the Multisikan EX with
onboard software (Ascent Software)
offers a wide range of data
reductions and report generations.
Absorbance Filters available: 405nm,
450nm, 540nm, and 620nm.
Applications: For reading 96-well
microplates, adapted ELISA and
other colorimetric assays and
Other applications include: Protein assays; Growth curve and hormone assays; Endotoxins; Food
diagnostics; HIV assays; Hybridization assays; Minisequencing assays; Cytotoxicity; Cell adhesion; Signal
transduction; Endotoxins; Antioxidants; Food diagnostics.
The Wellwash (SN: 006-1-10144) is a semiautomatic
microplate washer (96-well plates), comprising pump
and washer units. The instrument is supplied with
both 8- and 12-way interchangeable wash heads.
Applications: It can be used in research or routine-
test laboratories to wash microtiter microplates and
strips in 96-well plate format that meet the SBS
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GelDoc-It2 & ChemiDoc-It
The GelDoc-It2 Imager is a high resolution imager,
capable of capturing, documenting and analyzing
fluorescent gel images, chemiluminescent blot
images, including Western blots. The system is
equipped with a cooled, scientific-grade CCD
Image incorporate a light-tight darkroom with
VisionWorks software for image acquisition and
analysis functions. The full package VisionWorks
L.S. Image acquisition and Analysis Software (SN:
478457-8246UL-34DDT2-4906zz) is included for
use on an external computer. Analysis functions
include: band analysis, histograms, lane profile
graphs, total area density, Western blot
densitometry and data reporting.
The darkroom has a UV-blocking gel viewer
window, built-in overhead epi white lighting, a UV
transilluminator and a four-position emission
filter wheel with an ethidium bromide (EtBr)
emission filter included as standard.
Applications: capturing, documenting and analyzing fluorescent gel images, as well chemiluminescent blot
images, including Western blots.
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HL-2000 HybriLinker Hybridization Oven
Ultracentrifuge (Beckman Coulter; OptimaL-100xP)
Innovative HL-2000 HybriLinkerTM System (SN: 053003-002; CA
91786) is designed with the combination benefits of a
Hybridization Oven and UV Crosslinker (254nm UV) in one self-
contained, space saving unit for crosslinking prior to
The hybridization oven: is a cost-effective, high-performance,
10-bottle hybridization oven with microprocessor temperature
and variable speed controls. The Hybridizer can be set to
operate anywhere from 10 to 15 RPM. A touch-sensitive keypad
and microprocessor provide accurate temperature control from
ambient 10C to 99.9C. Two different hybridizer bottle sizes are
available for use within the hybridizer using the standard
carousel provided with the unit.
The crosslinker chamber: has shortwave 254nm, 8watt,
ultraviolet tubes supply uniform overhead UV illumination;
Preset and manual controls for ultraviolet or time exposures;
Preset exposure delivers 120,000 microjoules or five minutes of
exposure; Microprocessor measures and controls UV output.
Applications: Used for crosslinking of nucleic acids (DNA or RNA) to a nitrocellulose membrane, for hybridization
reactions in Southern and northern blotting assays, other UV Crosslinking.
Beckman Coulter; OptimaL-100xP ultracentrifuge
(SN: SERLxpo5G09; CA 94304) is capable of
generating maximum g- force 802,400xg (with a
maximum speeds up to 100,000 rpm0. Beckman
Coulters Ultracentrifuge enables researchers to
perform more separations in less time.
Applications: For proteomics, cytomics or
genomics, centrifugation of solutions containing:
RNA, DNA, microsomal p450 extraction and more.
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Cold Centrifuge-Centurion model K2015R Bench top
Cold centrifuge Hero lab GmbH (UniCen 15DR)
Cold Centrifuge-Centurion model K2015R Bench
top (SN: 217296-1): It has two rotors: BRK 5324 rotor
(capacity 24 x 2ml; For separation of solutions and
biological samples in a 1.5 ml tubes at 500-15000 rpm
at 40C - 250C) and BRK 5424 rotor (capacity 24 x 15 ml;
For separation of solutions and biological samples in
15 ml tubes at 500-6000 rpm at 40C - 250C).
HeroLab" Universal (Refrigerated) Benchtop Centrifuges
UniCen 15DR (SN: 0303715100): It is provided with two
rotors that can separate solutions and biological samples in
1.5-2 ml or 10 ml tubes at 5000 up to15300 rpm / 21,460 xg.
Temperature (Range from -10 to +40).
Applications of cold centrifuges: It is used to purify cells, subcellular organelles, viruses, proteins, and nucleic
acids (DNA and RNA).
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Thermo Scientific Heraeus Pico 17 Microcentrifuges
The Heraeus Pico17 (SN: 40651137) for routine laboratory work.
The 24-place rotor can be used for all 1.5 to 2.0 ml tubes, including
mini-preps and spin columns. It has a Max RCF 17,000 x g (Max
Speed 13,300 rpm); Process samples faster with unmatched
acceleration and deceleration rates.
Applications: For separation of solutions and biological samp