Rotor-Gene® Q (Qiagen) Real Time PCR Detection Files/Science/Biology... · Rotor-Gene® Q (Qiagen)

  • View
    220

  • Download
    0

Embed Size (px)

Text of Rotor-Gene® Q (Qiagen) Real Time PCR Detection Files/Science/Biology... · Rotor-Gene® Q (Qiagen)

  • 1 | P a g e

    Rotor-Gene Q (Qiagen) Real Time PCR Detection System

    Laboratory Name: Biochemistry & Molecular Biology Specialized Laboratory Lab Supervisor: Dr. Noura Abou Zeinab Location: Laboratory Building (A3), GF, Lab: G104-105, Debbieh Campus Tel. Ext.: 3363

    The Rotor-GeneQ instrument (SN: R121240)

    with its Software (Version 2.3.1.49) is designed to

    perform real-time and end-point thermal cycling

    using the polymerase chain reaction (PCR) and

    high-resolution melting analysis (HRM) in

    molecular biology applications.

    36-Well Rotor and 72-Well Rotor are available.

    with the instrument. 36-Well Rotor Locking Ring

    enables the use of 0.2 ml tubes. The 72-Well

    Rotor and Locking Ring are used with Strip Tubes

    and Caps, 0.1 ml, which can be used for volumes

    as low as 20 l. The caps provide a safe and

    reliable seal.

    Applications: It is highly suited to precisely

    quantitate and analyze changes in gene

    expression, genotyping, pathogen detection,

    concentration measurement, protein analysis,

    enzyme kinetics, and many other areas of

    research. The powerful and user-friendly software

    provides simplicity for beginners as well as an

    open experimental platform for advanced users. The 36-Well Rotor. The 72-Well Rotor.

    Applications for all thermal cyclers availabe (PX2, Q-Cycler, BIORAD)

    PCR is widely used as a diagnostic and research tool. Its applications are widespread over many disciplines,

    including molecular biology, microbiology, genetics, clinical diagnostics, forensic science, environmental science,

    hereditary studies and paternity testing.

    Copying and amplifying fragments of nucleic acids (DNA).

    Analyzing clinical specimens for the presence of infectious agents, e.g. HIV, hepatitis, human papillomavirus

    and others.

    In forensic science, using PCR-based DNA fingerprinting, fragments of DNA can be isolated from a crime scene

    and compared to a database of DNA of convicts or criminals.

    DNA fingerprinting is also used in paternity testing.

    Analysis of genetic mutations that occurs in cancer and many genetic diseases.

    Assisting the DNA sequencing technique where segments of DNA from an area of interest can be easily

    amplified to study their sequences.

    http://www.myvmc.com/diseases/hiv-aids-human-immune-deficiency-virus-acquired-immune-deficiency-syndrome/http://www.myvmc.com/?s=hepatitis&esect=Diseaseshttp://www.myvmc.com/diseases/human-papillomavirus-hpv/

  • 2 | P a g e

    C1000TM Thermal Cycler PCR BioRAD

    PCR Thermocycler Quanta Biotech Ltd (Q-Cycler)

    The C1000 cycler (184-1000; SN: cc008327) cycler.

    Dual 48/48 Reaction Module Its a Two Thermal

    Cyclers in One. It offers a gradient-enabled dual

    reaction module allowing to run two independent

    experiments at the same time. It is convenient for

    protocol optimization, allowing more variables to be

    tested simultaneously

    The Q-Cycler (7013000; SN: CM5057).

    The Q-Cycler II independently controls and displays a real

    time data for both itself and each connected satellite.

  • 3 | P a g e

    Thermo- Px2 Thermal Cycler (Thermo Electron Corporation)

    Thermo Electrons Px2 Thermal Cycler reliable PCR performance (SN: Px220328; Numelab Company) Simple to

    use, technologically advanced solution for thermal cycling

    with: Accurate temperature control for optimal PCR

    performance; Gradient capability for rapid protocol

    optimization; Outstanding well to well uniformity for

    reproducible PCR performance; High definition 1/4 VGA

    display for enhanced user-friendliness; Keypad with

    functional soft keys for easy software navigation

  • 4 | P a g e

    Thermo Lbsystems Multiskan EX - ELISA microplate reader

    Thermo Lbsystems Wellwash - ELISA washer

    The Thermo Scientic Multiskan EX

    (SN: 355-1-10183) is a basic reader

    for endpoint and kinetic assays.

    Additionally, the Multisikan EX with

    onboard software (Ascent Software)

    offers a wide range of data

    reductions and report generations.

    Absorbance Filters available: 405nm,

    450nm, 540nm, and 620nm.

    Applications: For reading 96-well

    microplates, adapted ELISA and

    other colorimetric assays and

    immunological reactions.

    Other applications include: Protein assays; Growth curve and hormone assays; Endotoxins; Food

    diagnostics; HIV assays; Hybridization assays; Minisequencing assays; Cytotoxicity; Cell adhesion; Signal

    transduction; Endotoxins; Antioxidants; Food diagnostics.

    The Wellwash (SN: 006-1-10144) is a semiautomatic

    microplate washer (96-well plates), comprising pump

    and washer units. The instrument is supplied with

    both 8- and 12-way interchangeable wash heads.

    Applications: It can be used in research or routine-

    test laboratories to wash microtiter microplates and

    strips in 96-well plate format that meet the SBS

    standards.

  • 5 | P a g e

    GelDoc-It2 & ChemiDoc-It

    2 Imagers

    The GelDoc-It2 Imager is a high resolution imager,

    capable of capturing, documenting and analyzing

    fluorescent gel images, chemiluminescent blot

    images, including Western blots. The system is

    equipped with a cooled, scientific-grade CCD

    camera.

    Image incorporate a light-tight darkroom with

    VisionWorks software for image acquisition and

    analysis functions. The full package VisionWorks

    L.S. Image acquisition and Analysis Software (SN:

    478457-8246UL-34DDT2-4906zz) is included for

    use on an external computer. Analysis functions

    include: band analysis, histograms, lane profile

    graphs, total area density, Western blot

    densitometry and data reporting.

    The darkroom has a UV-blocking gel viewer

    window, built-in overhead epi white lighting, a UV

    transilluminator and a four-position emission

    filter wheel with an ethidium bromide (EtBr)

    emission filter included as standard.

    Applications: capturing, documenting and analyzing fluorescent gel images, as well chemiluminescent blot

    images, including Western blots.

  • 6 | P a g e

    HL-2000 HybriLinker Hybridization Oven

    Ultracentrifuge (Beckman Coulter; OptimaL-100xP)

    Innovative HL-2000 HybriLinkerTM System (SN: 053003-002; CA

    91786) is designed with the combination benefits of a

    Hybridization Oven and UV Crosslinker (254nm UV) in one self-

    contained, space saving unit for crosslinking prior to

    hybridization protocols.

    The hybridization oven: is a cost-effective, high-performance,

    10-bottle hybridization oven with microprocessor temperature

    and variable speed controls. The Hybridizer can be set to

    operate anywhere from 10 to 15 RPM. A touch-sensitive keypad

    and microprocessor provide accurate temperature control from

    ambient 10C to 99.9C. Two different hybridizer bottle sizes are

    available for use within the hybridizer using the standard

    carousel provided with the unit.

    The crosslinker chamber: has shortwave 254nm, 8watt,

    ultraviolet tubes supply uniform overhead UV illumination;

    Preset and manual controls for ultraviolet or time exposures;

    Preset exposure delivers 120,000 microjoules or five minutes of

    exposure; Microprocessor measures and controls UV output.

    Applications: Used for crosslinking of nucleic acids (DNA or RNA) to a nitrocellulose membrane, for hybridization

    reactions in Southern and northern blotting assays, other UV Crosslinking.

    Beckman Coulter; OptimaL-100xP ultracentrifuge

    (SN: SERLxpo5G09; CA 94304) is capable of

    generating maximum g- force 802,400xg (with a

    maximum speeds up to 100,000 rpm0. Beckman

    Coulters Ultracentrifuge enables researchers to

    perform more separations in less time.

    Applications: For proteomics, cytomics or

    genomics, centrifugation of solutions containing:

    RNA, DNA, microsomal p450 extraction and more.

  • 7 | P a g e

    Cold Centrifuge-Centurion model K2015R Bench top

    Cold centrifuge Hero lab GmbH (UniCen 15DR)

    Cold Centrifuge-Centurion model K2015R Bench

    top (SN: 217296-1): It has two rotors: BRK 5324 rotor

    (capacity 24 x 2ml; For separation of solutions and

    biological samples in a 1.5 ml tubes at 500-15000 rpm

    at 40C - 250C) and BRK 5424 rotor (capacity 24 x 15 ml;

    For separation of solutions and biological samples in

    15 ml tubes at 500-6000 rpm at 40C - 250C).

    HeroLab" Universal (Refrigerated) Benchtop Centrifuges

    UniCen 15DR (SN: 0303715100): It is provided with two

    rotors that can separate solutions and biological samples in

    1.5-2 ml or 10 ml tubes at 5000 up to15300 rpm / 21,460 xg.

    Temperature (Range from -10 to +40).

    Applications of cold centrifuges: It is used to purify cells, subcellular organelles, viruses, proteins, and nucleic

    acids (DNA and RNA).

  • 8 | P a g e

    Thermo Scientific Heraeus Pico 17 Microcentrifuges

    The Heraeus Pico17 (SN: 40651137) for routine laboratory work.

    The 24-place rotor can be used for all 1.5 to 2.0 ml tubes, including

    mini-preps and spin columns. It has a Max RCF 17,000 x g (Max

    Speed 13,300 rpm); Process samples faster with unmatched

    acceleration and deceleration rates.

    Applications: For separation of solutions and biological samp