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Staining of Yeast Cells Author(s): Arthur P. Kelley and M. B. Shoemaker Source: Botanical Gazette, Vol. 83, No. 3 (May, 1927), p. 318 Published by: The University of Chicago Press Stable URL: http://www.jstor.org/stable/2470732 . Accessed: 28/06/2014 10:35 Your use of the JSTOR archive indicates your acceptance of the Terms & Conditions of Use, available at . http://www.jstor.org/page/info/about/policies/terms.jsp . JSTOR is a not-for-profit service that helps scholars, researchers, and students discover, use, and build upon a wide range of content in a trusted digital archive. We use information technology and tools to increase productivity and facilitate new forms of scholarship. For more information about JSTOR, please contact [email protected]. . The University of Chicago Press is collaborating with JSTOR to digitize, preserve and extend access to Botanical Gazette. http://www.jstor.org This content downloaded from 46.243.173.112 on Sat, 28 Jun 2014 10:35:29 AM All use subject to JSTOR Terms and Conditions

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Page 1: Staining of Yeast Cells

Staining of Yeast CellsAuthor(s): Arthur P. Kelley and M. B. ShoemakerSource: Botanical Gazette, Vol. 83, No. 3 (May, 1927), p. 318Published by: The University of Chicago PressStable URL: http://www.jstor.org/stable/2470732 .

Accessed: 28/06/2014 10:35

Your use of the JSTOR archive indicates your acceptance of the Terms & Conditions of Use, available at .http://www.jstor.org/page/info/about/policies/terms.jsp

.JSTOR is a not-for-profit service that helps scholars, researchers, and students discover, use, and build upon a wide range ofcontent in a trusted digital archive. We use information technology and tools to increase productivity and facilitate new formsof scholarship. For more information about JSTOR, please contact [email protected].

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The University of Chicago Press is collaborating with JSTOR to digitize, preserve and extend access toBotanical Gazette.

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This content downloaded from 46.243.173.112 on Sat, 28 Jun 2014 10:35:29 AMAll use subject to JSTOR Terms and Conditions

Page 2: Staining of Yeast Cells

3i8 BOTANICAL GAZETTE [MAY

STAINING OF YEAST CELLS

Methods of staining yeast cells have been collected to I9I5 by EYRE.,

Further improvements in technique have been made by DAVIS2 on spore staining which are applicable to yeasts, and by WINGARD3 in his study of the nuclear phenomena of the yeasts.

With large elementary classes, the smear method, whereby a drop of the culture is evaporated on a cover slip and fixed in the flame, usually results in shrinkage and distortion of the cells, and the cell content is not readily discernible. A method which is rapid and practicable for use in large classes has been devised in the botanical laboratory of Rutgers University. This involves the same principle as that used by DAVIS, who imbedded the cells in a thin film of parlodion on a slide, but requires less time. By it there results but little shrinkage of the cell, and vacuoles and protoplasmic structures are easily defined.

The procedure is as follows. On a clean glass slip place a drop of dilute albumin fixative (Mayer's albumin, diluted i: io with distilled water), stir in a small drop of the yeast culture and allow to dry over gentle heat (as on a warm radiator). The material should then be stained (acid fuchsin, acting for 50 seconds gives good results), the excess washed off, then dried as before, and mounted in balsam.-ARTHUR P. KELLEY and M. B. SHOEMAKER, Rutgers University, New Brunswick, N.J.

I EYRE, J. W. H., The elements of bacteriological technique. Pp. x+5i8., figs. 2i8. Philadelphia: W. B. Saunders Co. 1915.

2 DAVIS, W. H., Staining germinating spores. Phytopath. I2:492-494. I922.

3 WINGARD, S. A., Studies of the pathenogenicity, morphology and cytology of Nematospora Phaesoli. Torr. Bull. 52:249-290. I925.

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