Supplemental materials and methods...Aug 31, 2019 · Supplemental materials and methods Sample collection, preservation, and RNA extraction Specimens were preserved during field

Supplemental materials and methods

Sample collection, preservation, and RNA extraction

Specimens were preserved during field collections in RNAlater (Ambion) according to the

manufacturer’s recommendations, flash frozen at -80 C, or directly placed into 0.5 mL TRIzol for RNA

extraction, and total RNA was extracted with established protocols, homogenizing LN2-frozen

samples directly within 0.5-1.0 mL TRIzol (Riesgo et al., 2012b, 2012a). Tissues with RNA yields

expected to be within the sensitivity range of fragment analysis were routinely QC’d by Agilent

Bioanalyzer 2100, using RNA 6000 Pico or Nano kits (Agilent, Inc, cat. #s 5067-1511 and 5067-1513).

Polyadenylated mRNA was enriched using the Dynabeads™ mRNA Purification Kit (ThermoFisher,

Inc., cat. # 61006), eluting in up to 10 uL of 10 mM Tris-HCl, pH 7.5.

Transcriptome library generation

Except where specified, mRNA was converted into fragmented cDNA libraries and indexed

for Illumina sequencing using an automated library construction robot, the Apollo 324 (originally

manufactured by IntegenX Inc., then Wafergen, Inc., and currently by TaKaRa Bio, Inc). We used the

PrepX RNA-seq kit (TaKaRa, cat. # 640096), which RNAse III-fragments mRNA and performs single-

stranded adapter ligation prior to reverse transcription, to prepare up to 8 libraries at once, and

used manufacturer-provided indexing primers to assign sample barcodes. Libraries were QC’d using

the KAPA Library Quantification kit (KAPA Biosciences, Inc. KK4824, now Roche cat. # 07960140001),

and the Agilent 2100 High Sensitivity DNA kit (Agilent, Inc. cat. # 5067-4626). Pools were prepared

with plexity 6-12, and sequenced as paired-end 150 bp or 250 bp reads on Illumina HiSeq 2500

instruments, typically as rapid runs.

Whole genome and transcriptome amplified samples

For the gnathostomulid Tenuignathia rikerae, we attempted a genome assembly from DNA

amplified from a single individual by multiple displacement amplification. In brief, a single RNAlater

preserved specimen was rinsed in distilled water, and kept in a lens of ~2 μL, to which alkaline lysis

buffer from the Qiagen REPLI-g Single-Cell Kit (Qiagen, Inc., cat. # 150343) was directly added, and

DNA amplified as per manufacturer’s recommendations with the single-cell protocol, incubating at

30 C for 8 hours. MDA product was QC’d and confirmed to be HMW (70 kbp+) on an Agilent

Genomic DNA TapeStation 2200 kit (Agilent, Inc. cat. # 5067-5365). One aliquot was taken and

fragmented to ~350 bp using a Covaris S220 focused ultrasonicator and prepared as an Illumina

short-insert genomic DNA library using a previously described protocol for end-repair/A-tailing and

ligation of Y-shaped adapters (Neiman et al., 2012), followed by limited library amplification with

indexing primers and KAPA HiFi polymerase (KK2103). 1 μg of MDA product was processed into a

mate-pair library using the Illumina Nextera Mate-Pair Library Preparation Kit (Illumina, Inc. cat. #

FC-132-1001), following the manufacturer’s gel-free protocol, and amplifying the final Illumina

library with indexing oligos and the KAPA HiFi polymerase. Adapter sequences were trimmed from

the mate-pair library using NextClip (Leggett et al., 2014) using default parameters, and from the

short-insert library using Trimmomatic as described above. An initial assembly using SPAdes v3.2.0

was created running in single-cell mode; however, the assembly failed to finish at the k-mer size step

of 55. We therefore took the error-corrected reads output by SPAdes, and used these as inputs for

assembly with IDBA_UD v1.1.1 (Peng et al., 2012). The resulting assembly remained highly

fragmented (1,691,903 scaffolds of which 1,140,751 were

groups which contained more than 50 sequences and 50 columns. At this stage, we reconstituted

the orthogroups using the original, untrimmed sequences, and used the L-INS-I algorithm of the

MAFFT aligner (Katoh and Standley, 2013) to produce new multiple sequence alignment. These we

again masked with ZORRO, this time retaining all columns with a confidence score of > 0.5, and from

these masked alignments produced gene trees with RAxML, called with the PROTCATLGF model and

producing support values with 50 rapid bootstrap replicates. These gene trees and their originating

alignments were further curated with python scripts from the Phylogenomic Dataset Construction

(PDC) pipeline (Yang and Smith, 2014), calling the following three scripts in succession within the

working directory as such:

‘python trim_tips.py . .tre 5 10’ (removing all tree tips that were >5x longer than their sister, as well

as all tips that were over branch length 10)

‘python mask_tips_by_taxonID_transcripts.py . . y’ (masking all sequences from the same species

that formed a monophyletic or paraphyletic group, retaining the one with the longest proportion of

aligned residues)

‘python cut_long_internal_branches.py . tt.mm 2 30 cut_2’. (cutting all gene families into smaller

groups of size at least 30 if an edge within the gene tree had branch length of 2 or longer)

From this process survived 5,578 gene trees. These were then parsed to extract

unambiguously orthologous groups, using the criterion implemented in the Unrooted Phylogenetic

Orthology (UPhO) algorithm (Ballesteros and Hormiga, 2016), yielding 5,511 final orthogroups

comprising 195 taxa.

Matrix construction

To construct the pan-metazoa matrix, we selected a set of 422 alignments from the initial

set of 1,034 that were seen to have at least 100 sequences, by calling the MARE algorithm, initially

concatenating the 1,034 with phyutility’s ‘-concat’ option (Smith and Dunn, 2008), converting the

resulting NEXUS file into a FASTA in BioPython and extracting the partition list with vi regular

expressions, and running MARE v0.12 with the options ‘-t 100’ ‘-d 0.5’ to force retention of all taxa

and select for a matrix judged to be of adequate (initially at least 100,000 residues) size. Inspection

of this matrix and initial trees, and a contemporaneous publication reporting increased

compositional artifacts from including outgroups more distant than Choanoflagellata (Pisani et al.,

2015), led us to remove 28 taxa judged to be redundant representatives of their lineage and/or too

sparsely represented in the matrix, plus all non-choanoflagellate outgroups. The initial 422-gene

matrix of 106,186 residues was split into 10 nearly equally sized parts using BioPython, as a rough

parallelization of this job which ran very slowly as a serial process on the intact matrix, and BMGE

v1.12 was run with options ‘-g 0’, ‘-h 0.5’, ‘-m BLOSUM30’, and ‘-s FAST’ to retain all gapped sites,

but trim those that failed its approximate non-stationarity test, and those judged to be entropic

relative to the BLOSUM30 matrix (Criscuolo and Gribaldo, 2010). These parts were then re-joined

using BioPython to retain the final matrix. As reported in the main text, this process was performed

both before and after the deletion of the 28 taxa, with different results on the proportion of sites

retained.

For our subclade-specific matrices, we considered from among the set of 5,511 UPhO groups

a smaller set of 3,824 groups with at least 50 sequences, and created initial sets using the arbitrary

taxon inclusion criteria specified within the main text. MARE was used as described above to further

reduce these sets to matrix sizes deemed appropriate for CAT+GTR analysis (approximately 50-100k

sites), tuning the ‘-d’ parameter to effect this result, and the resultant matrix was then treated with

BMGE prior to phylogenetic analysis, again as called above.

Phylogenetic analyses

In general, CAT+GTR+Г4 analyses were conducted using the PhyloBayes-MPI v1.6j release (Lartillot et al., 2013), starting each chain without specifying an initial tree, specifying the model with ‘-cat’ ‘-gtr’, removing constant sites with the ‘-dc’ option, and running each generally with 10 to 20 MPI processes. Dayhoff-6 recoded analyses were conducted with the same default parameters, but in Phylobayes v4.1c serial (Lartillot et al., 2009), specifying ‘-dayhoff6’ to initiate recoding. To trim suspected rogue taxa, we first used the RogueNaRok algorithm (Aberer et al., 2013) with default parameters to inspect .treelist files, and removed the species thus identified using the ‘-prune’ option of phyutility, prior to a new summary. IQ-tree (Nguyen et al., 2015) was run using specified versions, typically as a multithreaded

process using ‘-nt 10’ or AUTO, and selecting ‘-bb 1000’ and ‘-bnni’ to run 1,000 UFboot2 replicates

corrected with nearest-neighbor interchanges. Models were specified with the ‘-m’ parameter as

described in figure captions; in general, whenever the PMSF model was applied (Wang et al., 2018),

it was used to approximate the C60+LG+FO+R4 profile mixture model, and was called using an initial

guide tree inferred with ‘-m LG4X+FO+R4’.

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Supplementary Figure and Table Captions

Figure S1 – Maximum Likelihood cladogram from concatenated analysis of all 3,824

trimmed orthologue alignments with greater than 50 species (201 taxa, 1,263,500

sites), inferred in IQ-tree v 1.6.3 under the LG4X+R4+FO model, with 1,000 UFboot2

replicates corrected with NNI.

Figure S2 – Maximum Likelihood cladogram from the pan-Metazoa matrix with no

taxon or site trimming (195 taxa, 106,186 sites), inferred in IQ-tree v1.6.2 under the

PMSF approximation of the C60+LG+FO+R4 model, with 1,000 UFboot2 replicates

corrected with NNI. In this and all other PMSF-profile derived trees, an initial guide

tree was inferred under the LG4X mixture model.

Figure S3 – Cladogram from posterior consensus summary of CAT+GTR+Γ4 analysis

of post-BMGE taxon-pruned, 43,011 site pan-Metazoa matrix, with rogue taxa masked

prior to summary. Two chains were used to form this consensus, sampling every 10

generations, discarding the first 2,000 as burn-in, yielding a maxdiff of 0.309244.


of post-BMGE taxon-pruned, 43,011 site pan-Metazoa matrix, with no rogue taxon

masking prior to summary. Two chains were used to form this consensus, sampling

every 10 generations, discarding the first 2,000 as burn-in, yielding a maxdiff of

0.309244.

Figure S5 – Cladogram from Maximum Likelihood analysis of the post-BMGE taxon-

pruned, 43,011 site pan-Metazoa matrix, inferred in IQ-tree v1.6.2 under the PMSF

approximation of the C60+LG+FO+R4 model, with 1000 UFboot2 replicates corrected

with NNI.


of pan-Metazoa matrix M, but with no rogue taxon masking prior to summary. Three

chains were used to form this consensus, sampling every 10 generations and

discarding the first 14,000 as burn-in, yielding a maxdiff of 0.21288.

Figure S7 - Cladogram from posterior consensus summary of CAT+GTR+Γ4 analysis

of pan-Metazoa matrix M, with rogue taxon masking prior to summary, and full pp and

taxon labels. Three chains were used to form this consensus, sampling every 10

generations and discarding the first 14,000 as burn-in, yielding a maxdiff of 0.21288.


of pan-Metazoa matrix M, recoded into in Dayhoff-6 groups, the same as presented in

Figure 2b, but with no rogue taxon masking prior to summary. Three chains were

used to form this consensus, sampling every 10 generations and discarding the first

2,100 as burn-in, yielding a maxdiff of 0.543937.


of pan-Metazoa matrix M, recoded into in Dayhoff-6 groups, the same as presented in

Figure 2b, with rogue taxon masking prior to summary. Three chains were used to

form this consensus, sampling every 10 generations and discarding the first 2,100 as

burn-in, yielding a maxdiff of 0.228175.

Figure S10 -- Maximum Likelihood cladogram derived from pan-Metazoa matrix M,

inferred in IQ-tree v1.6.2 under the PMSF approximation of the C60+LG+FO+R4 model,

with 1000 UFboot2 replicates corrected with NNI.

Figure S11 -- Cladogram from posterior consensus summary of CAT+GTR+Γ4

analysis of “Spiralia” matrix S, the same as presented in Figure 3, but with no rogue

taxon masking prior to summary. Two chains were used to form this consensus,

sampling every 10 generations and discarding the first 6,000 as burn-in, yielding a

maxdiff of 0.146158.


analysis of “Spiralia” matrix S, the same as presented in Figure 3, with rogue taxon

masking prior to summary, and full pp and taxon labels. Two chains were used to

form this consensus, sampling every 10 generations and discarding the first 6,000 as

burn-in, yielding a maxdiff of 0.140087.


analysis of “Spiralia” matrix S, recoded into Dayhoff-6 groups, with rogue taxon


every 10 generations and discarding the first 2,000 as burn-in, yielding a maxdiff of

0.0884638.

Figure S14 -- Maximum Likelihood cladogram derived from “Spiralia” matrix S,


with 1000 UFboot2 replicates corrected with NNI. The position of the root is arbitrarily

drawn between the clade of Syndermata+Micrognathozoa+Chaetognatha and the

remaining taxa.


analysis of “Ecdysozoa” matrix E, the same as presented in Figure 4, but with no

rogue taxon masking prior to summary. Two chains were used to form this

consensus, sampling every 10 generations and discarding the first 2,000 as burn-in,

yielding a maxdiff of 0.552004.


analysis of “Ecdysozoa” matrix E, the same as presented in Figure 4, with rogue

taxon masking prior to summary, and full pp and taxon labels. Two chains were used

to form this consensus, sampling every 10 generations and discarding the first 2,000

as burn-in, yielding a maxdiff of 0.0839663.


analysis of “Ecdysozoa” matrix E, recoded into Dayhoff-6 groups, with rogue taxon


every 10 generations and discarding the first 2,000 as burn-in, yielding a maxdiff of

0.0884638.

Figure S18 -- Maximum Likelihood cladogram derived from “Ecdysozoa” matrix E,


with 1000 UFboot2 replicates corrected with NNI.

Figure S19 -- Cladogram from posterior consensus summary of CAT+GTR+Γ4 amino

acid analysis of “Non-Bilateria” matrix N, the same as presented in Figure 5A. Two




Dayhoff-6 recoded analysis of “Non-Bilateria” matrix N, the same as presented in

Figure 5B. Two chains were used to form this consensus, sampling every 10


Figure S21 -- Cladogram from posterior consensus summary of CAT+GTR+Γ4 amino

acid analysis of “Non-Bilateria” matrix N’, the same as presented in Figure 5C. Two




Dayhoff-6 recoded analysis of “Non-Bilateria” matrix N’, the same as presented in

Figure 5D. Two chains were used to form this consensus, sampling every 10


Figure S23 – Cladogram from ML analysis of “Non-Bilateria” matrix N, inferred in IQ-

tree v1.6.2 under the PMSF approximation of the C60+LG+FO+R4 model, with 1000

UFboot2 replicates corrected with NNI.

Figure S24 – Cladogram from ML analysis of “Non-Bilateria” matrix N’, inferred in IQ-

tree v1.6.2 under the PMSF approximation of the C60+LG+FO+R4 model, with 1000

UFboot2 replicates corrected with NNI.

Table S1 – Mapping between full species name, abbreviated names used in Figures

S1-S24, assembly type, and SRA/Bioproject numbers/genome repository for raw data

source. Taxa annotated with * represent rogue taxa masked in Matrix M, those

annotated with † were deleted during the construction of Matrix M, and those

annotated with ‡ were taxa included in the orthology analysis, but which were not

included among the alignments with minimum 100 spp.

0.3

ANNE_Diur

NEME_Peeb

PLAT_Gapp

HEMI_Cgra

PHOR_Ppsa

OUTC_Mbre

PRIA_Pcau

CHAE_Selg

MOLL_Npom

MOLL_Mlab

ACOE_Isop

OUTF_Rsol

CRAN_Drer

HEMI_Tbah

ACOE_Hmia

ROTI_Rrot

NEMA_Hcon

CRAN_Mmus

PLAT_Ecmu

ANNE_Ofus

ACOE_Eumc

MOLL_Lvpl

NEME_Buni

OUTF_Mver

HEMI_Skow

ECHI_Spur

NEME_Hiji

ENTO_Bgra

ANNE_Mbel

NEME_Cham

NEMA_Celg

OUTF_Spun

PHOR_Paus

ARTH_Etai

ARTH_Dmel

PORI_Psub

OUTI_Apar

ANNE_Spsm

BRAC_Nvcn

CRAN_Hsap

BRAC_Gpyr

PRIA_Tubi

CHAE_Spdl

PLAC_Tadh

LORI_Arlc

BRYO_Hpac

CNID_Atet

MICR_Limn

CTEN_Vmul

CNID_AdigCNID_Nvec

ARTH_Ains

CTEN_Edun

ECHI_Pmin

PORI_Pfic

CTEN_Baby

HEMI_Sbra

PORI_Cvar

PLAT_Rros

CYCL_Symb

DICY_Dicy

KINO_Pkie

PLAT_Gnos

PORI_Ccan

PRIA_Meio

ARTH_Smar

TARD_HdEd

UROC_Odio

ECHI_Olon

ANNE_Phyl

CTEN_Pbac

ACOE_Sros

PLAT_Mfus

ANNE_Pamp

MOLL_Ovul

ECHI_Hgla

MOLL_Phol

PORI_Ccla

OUTI_Falb

ENTO_Lxmt

ACOE_Diog

CRAN_Pmar

ECHI_Lvar

ECHI_Ojap

OUTF_Rall

OUTF_Rirr

MOLL_Cgig

BRYO_Bstl

CNID_Smel

ANNE_Myzo

BRAC_Ttvs

PLAT_Sman

ANNE_Ssqu

HEMI_Pbah

CRAN_Ggal

ACOE_Diol

CNID_Csow

BRYO_Mmem

ARTH_Dpul

CRAN_Lcha

XENO_XbJC

ARTH_Amel

CRAN_Cmil

UROC_Cint

PORI_Avas

ONYC_Pcap

ARTH_Limu

NEMO_Mers

ANNE_Hrob

GAST_Megd

MOLL_Lott

ANNE_Loba

PHOR_Phar

GNAT_Augn

NEMO_Flag

ECHI_Etri

CNID_Phyd

CNID_Hvul

MOLL_Svel

TARD_Mtar

XENO_Xboc

ANNE_Pfoe

MOLL_Pcus

PLAT_Pvit

KINO_Eduj

ARTH_Smim

ROTI_Avag

CYCL_SyRN

GNAT_Trik

NEMA_Pred

ENTO_PclF

NEMA_Lloa

PORI_Scil

NEME_Aaus

CNID_Gven

OUTF_Amac

NEMM_Nect

ANNE_Ncae

MOLL_Cton

ARTH_Gmar

ROTI_Egad

NEMO_Asco

PORI_Aque

ROTI_Bcal

ARTH_Fcan

BRAC_Krub

ECHI_Oech

ONYC_Pept

CEPH_Aluc

ANNE_Mjoh

ACOE_Conv

PORI_Scoa

CTEN_Mlei

NEMA_Rcul

ROTI_Mhir

ONYC_Peri

PLAT_Mlin

ARTH_Scut

ACOE_Nfus

BRAC_LanaBRAC_Hpst

ARTH_Isca

GAST_Lepi

OUTI_Cowc

MOLL_Nmna

BRYO_Fcor

OUTI_Sart

MOLL_Lrug

OUTI_Ttra

PLAT_Sleu

OUTF_Foxy

GNAT_Gnat

PORI_CelePORI_Slac

NEMA_Tmur

ARTH_Fmer

NEMA_Tspi

OUTI_Mvar

GAST_Dpdl

ARTH_Cfin

GAST_Mdas

PORI_Ocar

GAST_Dnot

ECHI_Sbri

PORI_Cnuc

BRAC_Mcdv

ANNE_Plcs

ONYC_Oope

ANNE_Ctel

PLAT_Bsem

CEPH_Bflo

ACOE_Chil

NEMA_Ppac

OUTC_Sros

PLAT_Smed

ECHI_Ajap

NEMO_Nemw

ENTO_Loxp

ANNE_Lrub

MOLL_Hphy

PORI_Ifas

100

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100

100

100

100

100

100

100

100

100

100

100

100

100

100

100

100

100

100

100

100

100

100

100

100

100

100

100

100

100

100

100

100

100

100

100

100

100

100

100

100

100

100

100

100

100

100

100

100

100

100

100

100

100

100

100

100

100

100

100

100

100

100

100

100

100

100

100

100

100

100

100

100

100

100

100

100

S1

0.4

OUTF_Rall

HEMI_Sbra

NEME_Aaus

ARTH_Fcan

ARTH_Etai

ROTI_Avag

NEMO_Flag

CHAE_Selg

PRIA_Meio

ROTI_Egad

OUTI_Mvar

ARTH_Scut

BRYO_Mmem

OUTI_Cowc

ARTH_Isca

ARTH_Dpul

ANNE_Diur

ACOE_Hmia

CNID_Adig

NEMA_Ppac

NEMA_Celg

XENO_XbJC

UROC_Odio

NEMO_Mers

PORI_Cele

BRAC_Hpst

ECHI_Etri

ACOE_Isop

XENO_Xboc

CRAN_Cmil

CNID_Csow

ARTH_Smar

OUTF_Foxy

ROTI_Rrot

CRAN_Lcha

GAST_Mdas

CRAN_Drer

ONYC_Pcap

PHOR_Paus

MOLL_Nmna

MOLL_Phol

CRAN_Pmar

BRAC_Gpyr

PLAT_Smed

NEME_Peeb

NEMA_Lloa

ACOE_Chil

PLAC_Tadh

ENTO_PclF

MOLL_Lott

ECHI_Spur

PORI_Ocar

ANNE_Ncae

ONYC_Pept

ARTH_Cfin

ARTH_Ains

OUTI_Apar

MOLL_Mlab

MOLL_Lrug

PLAT_Rros

PLAT_Gapp

CEPH_Aluc

ECHI_Ajap

PORI_Psub

CNID_Atet

ARTH_Smim

OUTF_Mver

NEMO_Asco

MOLL_Pcus

ANNE_Spsm

ACOE_Sros

CTEN_Vmul

ACOE_Diog

NEMA_Pred

BRAC_Nvcn

MOLL_Svel

ONYC_Peri

MOLL_Cton

CRAN_Mmus

PHOR_Phar

GAST_Dnot

NEME_Buni

ECHI_Ojap

ECHI_Olon

ARTH_Gmar

PLAT_Ecmu

MOLL_Cgig

PLAT_Mlin

BRYO_Fcor

OUTC_Sros

ENTO_Lxmt

ROTI_Bcal

PORI_Scoa

HEMI_Tbah

KINO_Eduj

ANNE_Phyl

CTEN_Edun

PLAT_Sman

PLAT_Pvit

NEMA_Tmur

CNID_Hvul

OUTF_Spun

MOLL_Hphy

ECHI_Sbri

ECHI_Lvar

ANNE_Loba

MOLL_Lvpl

NEME_Hiji

CNID_Phyd

BRAC_Ttvs

CRAN_Hsap

CHAE_Spdl

MICR_Limn

TARD_Mtar

CYCL_Symb

ANNE_Ctel

CTEN_Mlei

CEPH_Bflo

ARTH_Amel

PORI_Aque

CTEN_Baby

ACOE_Eumc

ANNE_Ofus

TARD_HdEd

ARTH_Fmer

NEMA_Tspi

CNID_Nvec

HEMI_Cgra

OUTF_Amac

PORI_Ccan

ENTO_Bgra

NEMM_Nect

ECHI_Pmin

BRAC_Lana

PORI_Cvar

BRAC_Krub

MOLL_Ovul

PLAT_Mfus

LORI_Arlc

PORI_Cnuc

PORI_Ccla

ROTI_Mhir

ANNE_Lrub

GNAT_Trik

HEMI_Skow

PHOR_Ppsa

BRYO_Hpac

ECHI_Oech

ACOE_Conv

BRYO_Bstl

GAST_Lepi

ANNE_Pamp

NEMA_Rcul

OUTI_Ttra

PORI_Ifas

HEMI_Pbah

KINO_Pkie

ANNE_Hrob

MOLL_Npom

ENTO_Loxp

ACOE_Diol

NEMA_Hcon

NEME_Cham

OUTI_Falb

PLAT_Gnos

CNID_Smel

ANNE_Pfoe

ANNE_Mjoh

PLAT_Bsem

OUTI_Sart

ARTH_Limu

GNAT_Gnat

GAST_Dpdl

ANNE_Plcs

ANNE_Myzo

GNAT_Augn

ANNE_Mbel

CRAN_Ggal

GAST_Megd

PORI_Pfic

OUTC_Mbre

ARTH_Dmel

NEMO_Nemw

PORI_Scil

PORI_Slac

OUTF_Rsol

ECHI_Hgla

ONYC_Oope

CTEN_Pbac

UROC_Cint

PRIA_Pcau

CNID_Gven

ACOE_Nfus

PORI_Avas

PLAT_Sleu

100

100

100

100

100

100

93

100

100

100

100

100

100

100

100

100

100

100

100

100

100

100

100

100

92

36

100

100

100

91

100

100

100

100

100

100

100

100

100

100

100

100

97

100

100

100

100

100

64

100

100

100

100

100

97

100

100

64

100100

27

100

100

100

100

100

61

100

100

100

100

100

100

71

99

100

100

100

100

100

100

100

100

27

100

100100

100

99

100

100

94

100

100

100

100

99

100

14

100

100

100

100

100

100

100

95

98

100

100

100

100

100

100

96

100

62

93

100

100

100

100

100

100

100

88

100

100

100

100 100

100

100

100

100

100

100

100

100

100

67

100

100

100

100

100

100

100

59

1

100

100

100

100

9

100

100

100

100

100

100

100

98

35

100

100

100

100

67

100

100

83

100

100

100

100

100

59

100

100

23

100

100

100

100

62

100

100

100

100

100

100

100

S2

0.4

PLAT_Smed

ECHI_Sbri

NEMA_Lloa

PHOR_Ppsa

BRAC_Hpst

ANNE_Pfoe

MOLL_Npom

NEMA_Hcon

HEMI_Skow

PRIA_Meio

ACOE_Hmia

NEMA_Tmur

BRAC_LanaBRAC_Gpyr

ARTH_Etai

MOLL_Lott

ACOE_Chil

XENO_XbJC

ROTI_Rrot

GAST_Lepi

CRAN_Lcha

CNID_Hvul

ACOE_Conv

CNID_Gven

ANNE_Pamp

PLAT_Gnos

ACOE_Isop

PLAT_Rros

PORI_Slac

ARTH_Smim

NEMO_Nemw

MOLL_Nmna

ANNE_Mjoh

UROC_Cint

ARTH_Dmel

HEMI_Tbah

PLAT_Mlin

MOLL_Hphy

ROTI_Mhir

GAST_Mdas

ENTO_BgraCYCL_Symb

ROTI_Avag

PORI_Aque

NEMA_Pred

PORI_Cele

ARTH_Fcan

PORI_Cvar

PHOR_Phar

MOLL_Lrug

BRYO_Mmem

BRYO_Fcor

CNID_Phyd

ARTH_Dpul

MOLL_Mlab

CRAN_Drer

MOLL_Cgig

NEMO_Mers

PLAT_Bsem

PLAT_Mfus

ECHI_Etri

NEME_Aaus

PORI_Ccan

OUTC_Mbre

ARTH_Ains

CTEN_Mlei

CNID_Csow

MOLL_Ovul

CTEN_Edun

HEMI_Pbah

ECHI_Oech

ARTH_Gmar

PLAT_Ecmu

PORI_Psub

ECHI_Spur

ANNE_Mbel

GAST_Dnot

ACOE_Diog

GAST_Dpdl

OUTC_Sros

MOLL_Lvpl

MOLL_Svel

GNAT_Trik

NEMA_Ppac

ONYC_Oope

ECHI_Olon

PHOR_Paus

CRAN_Ggal

PLAT_Gapp

CEPH_Bflo

ARTH_Isca

ECHI_Pmin

BRYO_Hpac

CTEN_Baby

CNID_Smel

GAST_Megd

ONYC_Peri

NEMA_Tspi

CNID_Atet

CNID_Nvec

CRAN_Mmus

PLAT_Pvit

ROTI_Bcal

ANNE_Phyl

CTEN_Pbac

CHAE_Spdl

BRAC_TtvsBRAC_Krub

ARTH_Limu

ECHI_Lvar

ANNE_Ncae

PLAT_Sleu

ECHI_Ojap

GNAT_Augn

ARTH_Amel

ACOE_Nfus

ANNE_Lrub

MICR_Limn

ECHI_Ajap

NEME_Hiji

CHAE_Selg

PLAC_Tadh

TARD_Mtar

NEMO_Asco

NEME_Buni

NEME_Cham

CRAN_Hsap

ACOE_Sros

ANNE_Plcs

PORI_Scoa

ONYC_Pept

MOLL_Phol

NEMA_Rcul

PORI_Pfic

ENTO_Loxp

PORI_Cnuc

CEPH_Aluc

MOLL_Cton

ARTH_Fmer

HEMI_Cgra

CNID_Adig

NEMA_Celg

ACOE_Diol

CRAN_Pmar

PORI_Ocar

PRIA_Pcau

BRAC_Nvcn

NEME_Peeb

PORI_Ifas

ANNE_Ctel

PORI_Scil

HEMI_Sbra

ANNE_Hrob

ECHI_Hgla

MOLL_Pcus

CTEN_Vmul

ARTH_Cfin

BRYO_Bstl

PLAT_Sman

TARD_HdEd

ONYC_Pcap

CRAN_Cmil

ARTH_Smar

1

1

1

0.99

1

1

1

1

1

1

0.84

0.62

1

1

1

1

1

1

1

1

1

0.97

1

1

1

1

1

1

0.99

1

1

1

1

1

1

1

11

1

1

1

1

1

1

1

1

1

1

1

1

1

11

1

0.87

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

11

1

1

1

1

1

1

1

0.63

1

0.95

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

0.78

1

0.99

1

1

1

1

1

0.96

1

1

1

1

1

1

1

1

1

1

1

1

1

1

0.99

1

1

1

1

1

1

1

1

1

1

1

0.94

1

1

1

0.97

1

1

1

1

1

1

0.99

1

1

1

1

1

1

1

0.96

0.99

1

1

S3

0.4

CNID_Phyd

PHOR_Paus

NEMA_Tmur

ARTH_Isca

ACOE_Nfus

GNAT_Augn

BRYO_Bstl

BRAC_Ttvs

GAST_Lepi

BRAC_Lana

XENO_XbJC

PORI_Cvar

ECHI_Etri

PORI_Ccan

OUTC_Mbre

CRAN_Cmil

NEME_Peeb

ONYC_Oope

CRAN_Hsap

CNID_Adig

ANNE_Phyl

ARTH_Etai

ANNE_Mbel

PORI_Aque

ARTH_Ains

ACOE_Hmia

NEMA_Pred

ANNE_Ncae

TARD_Mtar

NEMM_Nect

PLAT_Rros

NEMA_Ppac

ACOE_Conv

PORI_Pfic

PLAT_Pvit

NEMA_Hcon

ECHI_Ajap

ARTH_Fcan

HEMI_Cgra

CRAN_Pmar

ANNE_Mjoh

MOLL_Cton

PORI_Ocar

PLAT_Mfus

ENTO_Loxp

CTEN_Edun

ANNE_Ctel

MOLL_Lvpl

PLAT_Smed

BRYO_Hpac

MOLL_Lott

HEMI_Skow

CTEN_Baby

ENTO_Bgra

BRAC_Nvcn

ANNE_Pamp

ONYC_Pcap

CNID_Smel

CTEN_Pbac

OUTC_Sros

GAST_Dnot

ARTH_Smim

ANNE_Plcs

TARD_HdEd

MICR_Limn

ANNE_Lrub

MOLL_Hphy

ACOE_Isop

PLAC_Tadh

ANNE_Pfoe

NEME_Cham

MOLL_Nmna

ARTH_Smar

CRAN_Lcha

ECHI_Lvar

ECHI_Hgla

BRYO_Mmem

CNID_Atet

HEMI_Pbah

CNID_Hvul

CRAN_Drer

PORI_Scoa

GNAT_Trik

PHOR_Ppsa

PHOR_Phar

ROTI_Mhir

PRIA_Pcau

PLAT_Ecmu

CEPH_Aluc

GAST_Mdas

PRIA_Meio

PORI_Psub

CNID_Csow

MOLL_Lrug

CRAN_Ggal

ECHI_Olon

ARTH_Dpul

ARTH_Gmar

NEME_Hiji

HEMI_Sbra

MOLL_Ovul

PLAT_Sleu

ACOE_Diol

NEMA_Tspi

BRAC_Krub

PLAT_Gapp

CHAE_Selg

PORI_Ifas

MOLL_Phol

CEPH_Bflo

GAST_Dpdl

CTEN_Vmul

ANNE_Hrob

NEMO_Mers

PLAT_Mlin

ACOE_Chil

MOLL_Mlab

PLAT_Sman

PORI_Scil

NEMO_Asco

CNID_Nvec

ARTH_Dmel

ECHI_Sbri

MOLL_Npom

BRAC_Gpyr

PORI_Cnuc

KINO_Pkie

UROC_Cint

ACOE_Sros

CNID_Gven

PORI_Cele

ARTH_Cfin

ARTH_Limu

BRAC_Hpst

PLAT_Bsem

NEMA_Rcul

ROTI_RrotROTI_Avag

NEME_Buni

CYCL_Symb

ARTH_Fmer

LORI_Arlc

HEMI_Tbah

MOLL_Cgig

KINO_Eduj

MOLL_Svel

NEME_Aaus

CHAE_Spdl

ECHI_Oech

GAST_Megd

ECHI_Ojap

MOLL_Pcus

ECHI_Pmin

ARTH_Amel

CRAN_Mmus

CTEN_Mlei

NEMA_Celg

PORI_Slac

PLAT_Gnos

NEMO_Nemw

ECHI_Spur

BRYO_Fcor

ACOE_Diog

ROTI_Bcal

ONYC_Peri

ONYC_Pept

NEMA_Lloa

1

1

1

1

1

1

0.99

1

0.82

1

1

1

0.97

1

1

1 1

0.86

0.78

1

1

1

1

1

1

1

0.85

1

1

0.79

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

0.96

1

1

1

1

1

1

1

1

1

1

1

0.94

0.95

0.62

1

0.63

1

1

1

1

1

1

1

1

1

0.74

1

1

1

0.99

1

1

1

0.99

0.99

1

1

1

1

1

0.95

1

1

1

1

1

1

1

1

1

1

0.96

1

1

1

1

1

1

1

0.99

1

1

0.84

0.94

1

1

1

1

0.97

1

1

1

1

1

1

1

1

1

1

1

0.99

1

1

1

1

1

0.72

1

1

0.87

1

1

1

S4

0.2

CNID_Atet

KINO_Pkie

CTEN_Edun

ARTH_Isca

ROTI_Avag

MOLL_Npom

PRIA_Pcau

ANNE_Pamp

ONYC_Peri

MOLL_Hphy

CNID_Csow

BRAC_Ttvs

ENTO_Bgra

PLAT_Ecmu

NEME_Aaus

PORI_Aque

NEME_Cham

PORI_Psub

BRYO_Hpac

ANNE_Hrob

PLAT_Bsem

ARTH_Amel

HEMI_Pbah

ARTH_Dmel

CNID_Smel

BRAC_Nvcn

PLAT_Mfus

CNID_Phyd

CTEN_Vmul

CEPH_Aluc

ECHI_Lvar

PORI_Slac

ANNE_Plcs

PLAT_Gapp

ACOE_Hmia

ONYC_Pept

BRAC_Krub

LORI_Arlc

ONYC_Pcap

NEMA_Lloa

CHAE_Spdl

CNID_Hvul

MOLL_Cton

NEMA_Rcul

ARTH_Fmer

PLAT_Sman

ANNE_Lrub

CHAE_Selg

ECHI_Etri

ACOE_Sros

PLAT_Rros

ANNE_Mbel

ARTH_Cfin

PORI_ScoaCTEN_Baby

ARTH_Ains

MOLL_Pcus

MOLL_Svel

GAST_Megd

PORI_Ocar

NEME_Hiji

ARTH_Gmar

PORI_Ifas

CTEN_Pbac

TARD_HdEd

OUTC_Mbre

KINO_Eduj

CNID_Gven

BRAC_Lana

ECHI_Olon

ARTH_Fcan

BRAC_Hpst

ONYC_Oope

NEMO_Mers

MOLL_Lrug

CRAN_Cmil

BRYO_Fcor

ANNE_Ctel

CNID_Nvec

GAST_Dnot

CRAN_Drer

NEMM_Nect

ARTH_Etai

ECHI_Hgla

CTEN_Mlei

NEMA_Tspi

ECHI_Sbri

HEMI_Sbra

PLAC_Tadh

PORI_Ccan

MOLL_Ovul

ARTH_Limu

ANNE_Mjoh

MOLL_Nmna

CRAN_Pmar

PORI_Cele

BRYO_Bstl

GNAT_Augn

MOLL_Lvpl

GAST_Lepi

PORI_Scil

CRAN_Mmus

MICR_Limn

ACOE_Conv

CNID_Adig

ACOE_Diol

PORI_Cnuc

ENTO_Loxp

PLAT_Mlin

MOLL_Cgig

ROTI_Bcal

CRAN_Hsap

ANNE_Ncae

UROC_Cint

OUTC_Sros

ACOE_Isop

NEMO_Asco

ARTH_Dpul

CEPH_Bflo

HEMI_Cgra

HEMI_Tbah

GNAT_Trik

PLAT_Gnos

NEMA_Celg

ARTH_Smar

ROTI_Rrot

PLAT_Pvit

BRYO_Mmem

GAST_Mdas

BRAC_Gpyr

CRAN_Lcha

ECHI_Ojap

MOLL_Phol

ECHI_Ajap

ACOE_ChilACOE_Nfus

ECHI_Pmin

PORI_Pfic

MOLL_Mlab

NEMA_Ppac

CYCL_Symb

PLAT_Sleu

PORI_Cvar

PLAT_Smed

PRIA_Meio

NEME_Peeb

CRAN_Ggal

ARTH_Smim

MOLL_Lott

NEME_Buni

ANNE_Phyl

XENO_XbJC

PHOR_Paus

NEMA_Tmur

ROTI_Mhir

ACOE_Diog

PHOR_Ppsa

NEMA_Pred

NEMO_Nemw

ANNE_Pfoe

GAST_Dpdl

NEMA_Hcon

TARD_Mtar

PHOR_Phar

ECHI_Oech

HEMI_Skow

ECHI_Spur

100

100

100

100

100

100

100

100

100

100

49

100

100

100

100

100

100

100

100

100

100

100

84

100

100

100

100

100

94

76

42

69100

100

91

100

100

100

100

100

99

100

94

68

57

68

100

100

100

100

100

67

33

100

98

100

100

73

99

100

100

100

66

100

100

99

86

100

100

61

100

100

51

56

100

100

100

100

100

100

68

100

100

27

100

100

100

100

49

100

100

100

100

100

100

100

100

91

100

100100

94

100

100

22

100

100

97

95

52

91

100

72

100

45

100

100

100

100

100

100

100

100

100

100

70

48

100

68

100

100

100

58

100

8190

78

90

100

100

100

100

100

100

30

72

88

100

100

100

100

51

100

100

100

87

100

100

100

52

100

66

96

100

92

S5

0.4

BRAC_Krub

XENO_XbJC

CYCL_SymbPLAT_Sman

GAST_Megd

LORI_Arlc

TARD_Mtar

NEME_Aaus

GNAT_Augn

ROTI_Bcal

ANNE_Hrob

PORI_Ocar

ECHI_Oech

NEMA_Pred

PLAT_Gnos

CTEN_Pbac

MOLL_Hphy

KINO_Pkie

NEME_Buni

PLAT_Smed

NEMA_Tmur

ECHI_Olon

ACOE_Isop

CRAN_Ggal

CNID_Hvul

ANNE_Phyl

ARTH_Gmar

NEMA_Tspi

PRIA_Pcau

ARTH_Cfin

NEMM_Nect

CNID_Nvec

PORI_Cvar

MOLL_Phol

CRAN_Drer

ARTH_Smim

CNID_Smel

ACOE_Hmia

PRIA_Meio

HEMI_Sbra

ANNE_Ctel

PORI_Aque

ONYC_Oope

CNID_Gven

MOLL_Lott

ARTH_Etai

BRAC_Gpyr

ECHI_Ojap

ACOE_Diol

PHOR_Phar

CRAN_Lcha

PLAT_Gapp

OUTC_Mbre

HEMI_Skow

GNAT_Trik

CRAN_Mmus

KINO_Eduj

CHAE_Spdl

MOLL_Cton

GAST_Mdas

PLAT_Pvit

CRAN_Pmar

PORI_Pfic

OUTC_Sros

PLAC_Tadh

BRYO_Hpac

ANNE_Ncae

ARTH_Amel

CTEN_Edun

MOLL_Npom

NEMO_Mers

PHOR_Paus

ONYC_Pcap

MOLL_Nmna

NEMA_Hcon

BRAC_Hpst

ECHI_Sbri

ANNE_Pamp

PORI_Cele

PLAT_Ecmu

ROTI_Rrot

TARD_HdEd

ACOE_Sros

ARTH_Isca

GAST_Dpdl

PLAT_Mfus

PLAT_Mlin

ACOE_Chil

PORI_Slac

CEPH_Aluc

ONYC_Pept

ACOE_Conv

BRAC_Nvcn

ANNE_Plcs

MOLL_Svel

NEMO_Nemw

NEMA_Ppac

BRYO_Fcor

CHAE_Selg

NEMA_Celg

ECHI_Spur

CEPH_Bflo

PORI_Psub

PORI_Scil

ACOE_Nfus

MOLL_Lrug

BRYO_Mmem

ARTH_Ains

PLAT_Rros

PLAT_Bsem

NEMA_Rcul

ONYC_Peri

ARTH_Fmer

CRAN_Hsap

ANNE_Lrub

BRYO_Bstl

UROC_Cint

ARTH_Fcan

MOLL_Mlab

ARTH_Dpul

CNID_Csow

CNID_Phyd

HEMI_TbahHEMI_Pbah

ANNE_Mbel

ACOE_Diog

PHOR_Ppsa

ROTI_Mhir

CTEN_MleiCTEN_Baby

ARTH_Dmel

ECHI_Lvar

ANNE_Pfoe

BRAC_Ttvs

CNID_Atet

MOLL_Ovul

PORI_Ccan

ROTI_Avag

HEMI_Cgra

ENTO_Loxp

ECHI_Hgla

MOLL_Pcus

CNID_Adig

NEMO_Asco

ANNE_Mjoh

PORI_Cnuc

ENTO_Bgra

PORI_Ifas

BRAC_Lana

ARTH_Smar

MOLL_Lvpl

GAST_Lepi

NEME_Cham

NEME_Peeb

ARTH_Limu

MICR_Limn

CRAN_Cmil

CTEN_Vmul

ECHI_Pmin

PLAT_Sleu

ECHI_Etri

ECHI_Ajap

PORI_Scoa

MOLL_Cgig

NEME_Hiji

GAST_Dnot

NEMA_Lloa

0.84

1

1

0.99

1

1

0.95

1

1

1

1

1

1

1

1

1

0.62

1

0.99

0.67

1

1

1

0.87

0.89

1

1

0.83

1

1

1

0.89

1

1

1

1

1

1

0.98

1

1

1

1

11

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

0.62

1

1

1

1

1

1

0.97

1

1

1

1

1

0.89

0.87

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

0.99

1

1

1

1

1

1

1

1

0.91

1

1

1

1

1

1

0.98

1

1

1

1

0.95

1

1

1

1

0.65

1

1

1

0.94

1

1

1

1

0.94

1

1

1

1

1

1

1

0.98

1

1

1

1

1

1

1

0.89

1

1

1

1

1

1

1

1

S6

ANNE_NcaeANNE_Mbel

PLAT_Rros

PRIA_Pcau

NEMA_Rcul

PLAT_Gnos

ECHI_Olon

ANNE_Hrob

NEMA_Ppac

MOLL_Cton

NEMO_Asco

OUTC_Sros

MOLL_Lvpl

BRYO_Fcor

GAST_Mdas

ECHI_Spur

PHOR_Phar

NEMO_Mers

PLAT_Mlin

PHOR_Ppsa

BRYO_Bstl

CTEN_Edun

CHAE_Spdl

ECHI_Etri

ACOE_Diog

CNID_Gven

CNID_Hvul

ECHI_Ajap

CNID_Smel

BRYO_Hpac

ARTH_Dmel

ARTH_Smar

BRYO_Mmem

ARTH_Etai

PORI_Cvar

PLAT_Bsem

PORI_Ccan

CNID_Nvec

ECHI_OjapHEMI_Tbah

MOLL_Lott

PLAT_SmanPLAT_Ecmu

PRIA_Meio

CRAN_Ggal

GAST_Dnot

TARD_HdEd

ONYC_Oope

ACOE_Nfus

HEMI_Skow

PORI_Cnuc

ANNE_Lrub

PORI_Slac

MOLL_Mlab

PLAT_Sleu

ANNE_Mjoh

PORI_Cele

PORI_Ocar

CYCL_Symb

CRAN_Lcha

ACOE_Isop

ROTI_Bcal

CTEN_Pbac

ROTI_RrotROTI_Avag

MOLL_Ovul

MOLL_Phol

NEMA_Celg

PORI_Pfic

ECHI_Pmin

ANNE_Plcs

ARTH_Amel

ENTO_Loxp

CRAN_Pmar

ROTI_Mhir

CNID_Phyd

NEME_Aaus

ACOE_Sros

ARTH_Ains

MICR_Limn

PLAT_Smed

BRAC_Lana

ANNE_Pfoe

NEMA_Tmur

ECHI_Hgla

CEPH_Bflo

NEMA_Hcon

NEMA_Lloa

CTEN_Baby

HEMI_Sbra

MOLL_Nmna

PHOR_Paus

GAST_Megd

CEPH_Aluc

HEMI_CgraCRAN_Mmus

XENO_XbJC

ACOE_Chil

BRAC_Nvcn

ARTH_Fcan

PORI_Psub

BRAC_Ttvs

ANNE_Pamp

MOLL_Pcus

ARTH_Cfin

ARTH_Limu

MOLL_Lrug

CRAN_Hsap

CTEN_MleiCTEN_Vmul

GAST_Lepi

CRAN_Cmil

CNID_Adig

ARTH_Fmer

BRAC_Gpyr

ONYC_Peri

PORI_Ifas

CNID_Csow

ECHI_Sbri

ECHI_Lvar

ONYC_Pcap

CRAN_Drer

PORI_Aque

MOLL_Hphy

ACOE_Hmia

BRAC_Krub

MOLL_Cgig

UROC_Cint

TARD_Mtar

ANNE_Phyl

BRAC_Hpst

NEMA_Pred

PLAC_Tadh

PORI_Scil

ECHI_Oech

ACOE_Diol

ONYC_Pept

NEMO_Nemw

NEME_Cham

NEME_Buni

MOLL_Svel

NEME_Peeb

NEMA_Tspi

CHAE_Selg

ACOE_Conv

HEMI_Pbah

GNAT_Trik

PLAT_Gapp

ENTO_Bgra

OUTC_Mbre

ANNE_Ctel

CNID_Atet

PORI_Scoa

PLAT_Mfus

ARTH_Gmar

GAST_Dpdl

GNAT_Augn

PLAT_Pvit

MOLL_Npom

NEME_Hiji

ARTH_Isca

1

0.71

1

1

0.92

1

1

1

1

1

1

1

1

1

1

1

0.97

1

1

1

1

1

1

1

1

0.83

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

0.97

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

11

1

1

1

1

1

0.76

1

1

0.5

1

1

0.69

1

1

1

1

1

1

1

0.88

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

0.91

1

1

1

1

1

1

1

1

0.87

0.95

0.9

0.92

1

1

1

1

1

1

0.99

1

1

0.86

1

1

1

1

1

1

0.96

1

1

1

1

1

1

1

1

1

1

1

0.52

1

1

1

1

1

1

1

1

1

1

1

0.87

0.96

1

S7

ROTI_Mhir

PRIA_Meio

NEMA_Tmur

BRYO_Mmem

PHOR_Ppsa

MOLL_Phol

ECHI_Lvar

OUTC_Sros

ACOE_Hmia

GAST_Mdas

PLAT_Gapp

ROTI_Bcal

ARTH_Etai

ACOE_Isop

PLAT_Smed

NEME_Cham

ECHI_Etri

BRAC_Gpyr

ECHI_Sbri

CNID_Nvec

ROTI_Rrot

CRAN_Lcha

MOLL_Svel

CYCL_Symb

PLAT_Mfus

ECHI_Pmin

ANNE_Pamp

BRYO_Hpac

CTEN_BabyCTEN_Mlei

CHAE_Spdl

PLAC_Tadh

ENTO_Loxp

ECHI_Oech

ARTH_Amel

ANNE_PlcsANNE_Phyl

LORI_Arlc

KINO_Eduj

CTEN_Pbac

PORI_Psub

PLAT_Mlin

ECHI_Ajap

CNID_Phyd

ECHI_Hgla

NEMA_Hcon

MOLL_Lrug

ANNE_Mjoh

BRAC_Hpst

PLAT_Bsem

CRAN_Drer

CEPH_AlucACOE_Sros

PORI_Scoa

PLAT_Rros

CNID_Gven

PLAT_Sleu

NEME_Buni

ARTH_Fmer

NEMA_Tspi

PORI_Ifas

ONYC_Oope

CEPH_Bflo

BRYO_Fcor

MICR_Limn

ARTH_Isca

HEMI_Cgra

TARD_HdEd

GAST_Dpdl

XENO_XbJC

GAST_Dnot

ONYC_Pept

CNID_Atet

MOLL_Ovul

ACOE_Conv

BRAC_Ttvs

ACOE_Chil

ECHI_Ojap

CRAN_Mmus

PORI_Scil

GNAT_Augn

MOLL_Cton

NEME_Peeb

ACOE_Diog

PORI_Slac

ECHI_Spur

PLAT_Gnos

NEMA_Ppac

MOLL_Lott

MOLL_Nmna

CHAE_Selg

CTEN_Edun

PHOR_Paus

TARD_Mtar

CNID_Csow

ECHI_Olon

ARTH_Smar

ACOE_Nfus

PRIA_Pcau

ARTH_Limu

NEMM_Nect

MOLL_Mlab

ONYC_Pcap

ACOE_Diol

MOLL_Pcus

NEMO_Nemw

ANNE_Mbel

PLAT_Sman

HEMI_Sbra

MOLL_Npom

ANNE_Ncae

MOLL_Lvpl

PHOR_Phar

BRAC_Krub

PORI_Ccan

NEMA_Pred

HEMI_Tbah

ANNE_Ctel

ARTH_Fcan

NEME_Aaus

PORI_Cvar

ANNE_Pfoe

CRAN_Pmar

HEMI_Skow

ARTH_Cfin

CRAN_Cmil

CRAN_Ggal

UROC_Cint

NEMO_Mers

ARTH_Dmel

ARTH_Ains

CNID_Smel

NEMA_Celg

BRAC_Nvcn

PORI_Pfic

CNID_Hvul

HEMI_Pbah

CTEN_Vmul

PLAT_Pvit

ONYC_Peri

BRYO_Bstl

MOLL_Hphy

BRAC_Lana

NEMA_Lloa

CNID_Adig

NEME_Hiji

ARTH_Gmar

OUTC_Mbre

ENTO_Bgra

CRAN_Hsap

GAST_Lepi

PORI_Cnuc

NEMA_Rcul

NEMO_Asco

ARTH_Smim

ROTI_Avag

PLAT_Ecmu

GAST_Megd

GNAT_Trik

PORI_Ocar

ANNE_Lrub

KINO_Pkie

PORI_Cele

MOLL_Cgig

ARTH_Dpul

PORI_Aque

ANNE_Hrob

1

1

1

0.68

1

1

0.86

1

1

1

0.9

1

1

1

1

1

1

1

0.97

1

0.99

1

1

1

0.69

1

1

1

1

0.84

1

1

1

0.85

1

1

0.661

1

1

1

1

1

0.88

1

0.99

1

1

1

1

0.95

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

0.97

1

1

1

0.93

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

0.51

1

1

1

0.89

0.96

0.99

1

1

1

1

1

11

0.88

1

10.53

0.81

1

1

0.65

1

0.98

1

1

1

1

1

1

1

1

1

1

0.96

1

1

1

1

1

1

1

1

1

1

1

0.91

1

0.72

1

0.75

1

0.52

S8

0.4

PLAT_Sman

NEME_Cham

PORI_Cele

PHOR_Paus

ANNE_Ncae

ANNE_Hrob

MOLL_Lvpl

CTEN_Pbac

GAST_Megd

ANNE_Mbel

ARTH_Cfin

ONYC_Peri

OUTC_Mbre

MOLL_Mlab

BRAC_Nvcn

CTEN_Mlei

MOLL_Npom

PLAT_Rros

GNAT_Trik

PHOR_Ppsa

NEME_Buni

PORI_Scil

NEME_Peeb

BRAC_Gpyr

PLAT_Sleu

CNID_Atet

NEME_Aaus

ARTH_Smar

PORI_Scoa

MOLL_Cgig

PLAC_Tadh

CRAN_Cmil

PLAT_Gapp

ANNE_Pfoe

PRIA_Meio

ANNE_Ctel

ACOE_Isop

TARD_HdEd

CNID_Csow

PORI_Slac

BRYO_Bstl

NEMA_Hcon

MOLL_Pcus

MOLL_Ovul

ARTH_Fcan

BRYO_Hpac

MOLL_Nmna

NEMA_Rcul

CNID_Smel

PLAT_Gnos

MOLL_Hphy

ARTH_Amel

MOLL_Phol

ENTO_Loxp

MOLL_Lrug

ACOE_Sros

CTEN_Vmul

CYCL_Symb

CRAN_Mmus

ECHI_Olon

CTEN_Edun

BRAC_Hpst

PORI_Ocar

PLAT_Smed

ANNE_Phyl

ARTH_Fmer

ONYC_Pcap

ARTH_Etai

CNID_Nvec

NEMA_Ppac

ECHI_Ojap

ACOE_Diol

ROTI_Mhir

ECHI_Oech

ARTH_Isca

GAST_Lepi

CRAN_Ggal

PLAT_Ecmu

GNAT_Augn

ECHI_Lvar

ANNE_Pamp

GAST_Mdas

ARTH_Dpul

ACOE_Diog

ANNE_Lrub

PORI_Ifas

ANNE_Mjoh

MICR_Limn

HEMI_Sbra

CHAE_Spdl

ARTH_Dmel

NEMA_Lloa

TARD_Mtar

ECHI_Ajap

PLAT_Pvit

ACOE_Chil

NEMA_Tmur

BRAC_Lana

CNID_Hvul

NEMA_Tspi

PORI_Cvar

PLAT_Bsem

NEMA_Pred

BRYO_Fcor

ANNE_Plcs

ENTO_Bgra

ARTH_Gmar

ARTH_Smim

ECHI_Sbri

ROTI_Avag

BRYO_Mmem

ECHI_Pmin

HEMI_Pbah

NEMA_Celg

CEPH_Bflo

MOLL_Cton

HEMI_Skow

GAST_Dnot

ECHI_Spur

PLAT_Mlin

PORI_Ccan

PORI_Pfic

UROC_Cint

ONYC_Pept

CRAN_Drer

CTEN_Baby

PHOR_Phar

OUTC_Sros

BRAC_Krub

NEMO_Asco

BRAC_Ttvs

CHAE_Selg

NEME_Hiji

ARTH_Limu

ACOE_Conv

NEMO_Mers

PORI_Psub

MOLL_Svel

ONYC_Oope

HEMI_Tbah

ECHI_Hgla

PORI_Cnuc

CRAN_Hsap

MOLL_Lott

GAST_Dpdl

PORI_Aque

ACOE_Nfus

ECHI_Etri

XENO_XbJC

PRIA_Pcau

ACOE_Hmia

CNID_Adig

CRAN_Pmar

CNID_Gven

CEPH_Aluc

ROTI_Rrot

ROTI_Bcal

CNID_Phyd

CRAN_Lcha

HEMI_Cgra

PLAT_Mfus

ARTH_Ains

NEMO_Nemw

1

1

1

0.84

1

1

1

1

0.99

1

1

0.91

1

1

1

1

1

1

1

1

0.99

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

0.87

1

0.89

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

0.64

1

1

1

0.98

1

1

1

1

1

1

1

1

1

1

0.92

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

0.98

0.88

1

1

1

1

1

1

1

1

1

1

1

1

0.68

1

1

0.89

1

1

1

1

1

1

1

1

1

1

1

1

1

0.98

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

S9

0.2

MOLL_Npom

BRYO_Fcor

ROTI_Mhir

MOLL_Mlab

BRYO_Mmem

NEME_Buni

HEMI_Skow

ECHI_Etri

PORI_Cnuc

PORI_Scil

ANNE_Phyl

ACOE_Nfus

NEMO_Mers

PORI_Cele

GAST_Mdas

NEMM_Nect

XENO_XbJC

ANNE_Ctel

PLAT_Gnos

ROTI_Bcal

GAST_Dpdl

CRAN_Ggal

CNID_Nvec

PORI_Psub

PLAT_Rros

MOLL_Nmna

PLAT_Smed

ENTO_Loxp

CRAN_Cmil

CNID_Hvul

ANNE_Hrob

PORI_Ocar

NEME_Peeb

PLAT_Pvit

ARTH_Amel

ARTH_Ains

ACOE_Conv

ARTH_Dpul

MOLL_Ovul

CNID_Smel

GAST_Megd

PORI_Pfic

CNID_Phyd

LORI_Arlc

ONYC_Oope

ACOE_Chil

NEMA_Ppac

ECHI_Sbri

ANNE_Mjoh

ECHI_Ajap

PORI_Ifas

CYCL_Symb

ARTH_Isca

ANNE_Pfoe

PLAT_Mfus

BRAC_Hpst

CRAN_Drer

PLAT_Ecmu

NEMO_Nemw

ONYC_Peri

NEMA_Tmur

ACOE_Diog

NEMA_Pred

KINO_Eduj

ECHI_Oech

ACOE_Isop

MOLL_Hphy

HEMI_Tbah

PLAT_Mlin

NEMA_Rcul

ARTH_Etai

CTEN_Mlei

NEMA_Hcon

PRIA_Pcau

ENTO_Bgra

HEMI_Sbra

ACOE_Diol

NEMA_Lloa

MOLL_Cton

CRAN_Mmus

MOLL_Lott

PLAT_Bsem

PORI_Ccan

OUTC_Sros

PLAT_Gapp

ECHI_Spur

ANNE_Mbel

ECHI_Ojap

ARTH_Gmar

BRAC_Lana

CTEN_Pbac

CRAN_Hsap

ECHI_Pmin

ROTI_Avag

CNID_Atet

ACOE_Sros

PRIA_Meio

BRYO_Hpac

NEME_ChamBRAC_Gpyr

ECHI_Olon

ANNE_Plcs

NEME_Hiji

PLAC_Tadh

MOLL_Lrug

HEMI_Cgra

ANNE_Pamp

PHOR_Phar

PORI_Cvar

GNAT_Trik

ARTH_Fmer

ARTH_Dmel

PORI_Scoa

ECHI_Hgla

PLAT_Sleu

CHAE_Selg

CTEN_Baby

ARTH_Cfin

ONYC_Pept

BRAC_Krub

ARTH_Smar

CRAN_Lcha

CHAE_Spdl

ANNE_Ncae

CNID_Gven

OUTC_Mbre

ANNE_Lrub

PORI_Slac

PHOR_Paus

ACOE_Hmia

NEME_Aaus

UROC_Cint

CNID_Adig

PLAT_Sman

BRYO_Bstl

CEPH_Bflo

MOLL_Cgig

HEMI_Pbah

TARD_HdEdTARD_Mtar

CNID_Csow

CTEN_Vmul

BRAC_Nvcn

CEPH_Aluc

ONYC_Pcap

MOLL_Svel

ARTH_Fcan

GNAT_Augn

NEMO_Asco

MOLL_Lvpl

ECHI_Lvar

CRAN_Pmar

ARTH_Smim

NEMA_Tspi

KINO_Pkie

GAST_DnotGAST_Lepi

PORI_Aque

CTEN_Edun

BRAC_Ttvs

MOLL_PcusMOLL_Phol

MICR_Limn

PHOR_Ppsa

ROTI_Rrot

ARTH_Limu

NEMA_Celg

88

100

75

100

100

100

85

100

100

100

73

100

100

100

74

97

99

100

100

19

100

100

97

78

100

100

100

100

100

77

100

67

100

79

100

100

49

100

100

100

100

79

90

100

100

100

100

91

91

100

88

100

94

100

100

100

100

85

78

100

100

12

100

100

100

100

100

100

98

89

100

100

100

100

100

57

100

93

100

100

100

99

93

100

58

49

100

100

45

100

30

100

53

95

100

100

100

100

100

65

100

99

97

100

63

100

96

100

44

100

100

99

84

100

100

100

99

100

100

100

71

100

100

100

100

100

100

88

100

100100

100

100

100

100

100

82

100

100

100

100

100

100

100

100

100

72

100

100

100

100

100

100

100

100

100

70

100

100

100

15

87

100

100

100

S10

0.4

PLAT_Pvit

GAST_DnotROTI_Mhir

ROTI_Rrot

BRAC_Lana

MOLL_Lvpl

PLAT_Sleu

PLAT_Smed

ENTO_PclF

ANNE_Pfoe

NEME_Peeb

PHOR_Paus

ROTI_Egad

MICR_Limn

BRYO_Fcor

ANNE_Ssqu

ANNE_Myzo

GAST_Mdas

CHAE_Spdl

BRYO_Bstl

PLAT_SmanPLAT_Ecmu

PLAT_Mlin

ANNE_Mbel

ANNE_Loba

ANNE_Plcs

MOLL_Npom

GAST_Dpdl

NEME_Aaus

PLAT_Gapp

BRAC_Ttvs

ANNE_Phyl

ANNE_Hrob

BRAC_Hpst

MOLL_Mlab

GAST_Lepi

PLAT_Gnos

ANNE_Ofus

ANNE_Ctel

BRAC_Mcdv

ENTO_Bgra

MOLL_Svel

GAST_Megd

GNAT_Trik

ANNE_Pamp

PHOR_Ppsa

CHAE_Selg

MOLL_Cton

BRAC_Nvcn

MOLL_Lott

NEME_Hiji

BRYO_Hpac

MOLL_Phol

ANNE_Ncae

BRAC_Krub

PLAT_Rros

ANNE_Diur

ANNE_Mjoh

MOLL_Lrug

CYCL_Symb

PHOR_Phar

MOLL_Cgig

GNAT_Augn

ENTO_Lxmt

NEME_Cham

ROTI_Avag

MOLL_Hphy

PLAT_Mfus

NEME_Buni

BRYO_Mmem

MOLL_Ovul

ROTI_Bcal

GNAT_Gnat

MOLL_Pcus

ENTO_Loxp

BRAC_Gpyr

PLAT_Bsem

ANNE_Spsm

ANNE_Lrub

MOLL_Nmna

0.92

0.88

0.89

1

1

0.98

1

1

0.97

0.9

1

1

0.94

0.78

0.99

1

0.66

1

1

0.98

1

0.99

1

0.79

0.98

1

1

0.99

0.95

1

1

0.98

0.95

0.99

1

0.9

0.99

0.98

0.85

1

0.74

0.98

0.52

0.99

0.68

1

0.84

0.91

0.97

1

0.97

1

0.8

1

1

0.78

0.96

0.86

1

0.9

0.89

1

1

1

0.86

0.96

0.99

1

0.52

0.63

1

1

1

0.98

0.99

1

S11

0.4

MOLL_Phol

PHOR_Phar

GAST_Dpdl

BRYO_Hpac

ROTI_Bcal

ANNE_Lrub

ANNE_Phyl

GAST_Lepi

GAST_Mdas

MOLL_LottMOLL_HphyMOLL_Mlab

ANNE_Pfoe

ENTO_Lxmt

PLAT_Mfus

GNAT_Augn

MOLL_Cgig

GNAT_Trik

PLAT_Gnos

ANNE_Diur

GNAT_Gnat

GAST_Megd

MOLL_Lvpl

ROTI_Mhir

CHAE_Selg

BRAC_Gpyr

PLAT_Bsem

ANNE_Ncae

BRYO_Fcor

MOLL_Npom

NEME_Aaus

BRYO_Mmem

PLAT_Sman

ROTI_Avag

MOLL_Cton

PHOR_Paus

ENTO_PclF

PLAT_Smed

ANNE_OfusANNE_Mjoh

PLAT_Ecmu

ROTI_Egad

ANNE_Pamp

MOLL_Pcus

ANNE_Plcs

MICR_Limn

ROTI_Rrot

MOLL_Nmna

PHOR_Ppsa

BRAC_Krub

ANNE_Ctel

PLAT_Rros

ANNE_Spsm

ANNE_Mbel

NEME_Cham

ENTO_BgraCYCL_Symb

PLAT_Gapp

NEME_BuniNEME_Hiji

MOLL_Lrug

PLAT_Sleu

BRYO_Bstl

BRAC_Lana

MOLL_Svel

PLAT_Pvit

CHAE_Spdl

BRAC_Nvcn

ENTO_Loxp

NEME_Peeb

MOLL_Ovul

PLAT_Mlin

GAST_Dnot

ANNE_Hrob

ANNE_Myzo

BRAC_Ttvs

BRAC_Hpst

ANNE_Loba

1

1

1

0.91

1

1

1

1

1

1

0.92

1

1

0.99

1

1

1

1

1

1

1

1

1

1

1

0.89

1

1

1

1

1

1

1

1

1

1

11

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

0.93

0.94

0.98

1

1

1

1

1

1

1

1

0.99

0.92

1

1

1

0.92

S12

0.2

PHOR_Ppsa

ENTO_Loxp

ANNE_Mbel

MOLL_Mlab

BRAC_Hpst

ROTI_Rrot

ANNE_Ncae

PLAT_PvitPLAT_Gapp

PLAT_Bsem

ANNE_Plcs

MOLL_Cton

GNAT_Gnat

PHOR_Paus

ROTI_Egad

MOLL_Lott

ANNE_Hrob

GAST_Megd

ANNE_Myzo

NEME_Aaus

MOLL_Hphy

BRAC_Ttvs

ANNE_Mjoh

ANNE_Ctel

BRAC_Gpyr

ANNE_Diur

NEME_Hiji

CYCL_Symb

BRAC_Lana

ANNE_Pfoe

MOLL_Npom

PLAT_Mfus

NEME_Peeb

CHAE_Selg

PLAT_Rros

MOLL_Pcus

ROTI_Avag

PLAT_Mlin

BRYO_Hpac

BRAC_Nvcn

CHAE_Spdl

ANNE_Loba

PLAT_Ecmu

GAST_Lepi

ROTI_Bcal

ENTO_Lxmt

ANNE_Lrub

ANNE_Spsm

ANNE_PhylANNE_Pamp

MOLL_Phol

GAST_Dpdl

MOLL_Lvpl

BRYO_Mmem

PHOR_Phar

MOLL_Svel

GAST_Dnot

PLAT_Smed

ANNE_Ofus

PLAT_Gnos

MOLL_Cgig

ENTO_PclF

GNAT_Augn

BRAC_Krub

BRYO_BstlBRYO_Fcor

PLAT_Sleu

ENTO_Bgra

MOLL_Ovul

MOLL_Nmna

GNAT_Trik

NEME_Buni

PLAT_Sman

ROTI_Mhir

MOLL_Lrug

MICR_Limn

GAST_Mdas

NEME_Cham

1

1

1

1

1

1

1

1

1

0.97

1

1

1

0.53

1

1

0.97

1

0.55

1

0.74

0.92

0.86

1

0.87

0.89

0.79

1

1

1

1

0.99

1

0.87

1

0.56 1

0.73

1

1

1

1

1

1

1

1

1

1

1

0.98

1

1

1

0.91

1

1

1

1

1

1

1

1

1

1

0.99

1

1

0.84

0.761

0.83

1

0.99

1

1

1

S13

0.2

MOLL_Cgig

NEME_Cham

ANNE_Ofus

GAST_Mdas

ANNE_Mjoh

MOLL_Cton

BRAC_Gpyr

MOLL_Lrug

NEME_Aaus

ENTO_Bgra

ANNE_Hrob

MOLL_Mlab

ENTO_Loxp

ANNE_Diur

ANNE_Spsm

BRAC_Mcdv

ANNE_Lrub

ENTO_Lxmt

ANNE_Pamp

BRYO_Mmem

PLAT_Mlin

CHAE_Selg

PLAT_Pvit

ANNE_Loba

PLAT_Bsem

BRAC_Krub

PLAT_Sman

PLAT_Gapp

NEME_Hiji

MOLL_Phol

CYCL_SymbPLAT_Ecmu

PLAT_Smed

GAST_Dnot

PHOR_Paus

MOLL_Npom

BRAC_Lana

BRYO_Bstl

PLAT_Gnos

PLAT_Sleu

ANNE_Plcs

ANNE_MbelANNE_Ncae

GNAT_Trik

MICR_Limn

PHOR_Ppsa

GNAT_Gnat

GAST_Dpdl

GAST_Lepi

ANNE_Phyl

PLAT_Rros

ROTI_Avag

BRYO_Hpac

BRAC_Hpst

NEME_Buni

PHOR_Phar

MOLL_Ovul

MOLL_Lott

ANNE_Ctel

ROTI_Bcal

MOLL_Lvpl

MOLL_Nmna

ANNE_Ssqu

BRAC_Ttvs

BRAC_Nvcn

PLAT_Mfus

ANNE_Pfoe

NEME_Peeb

BRYO_Fcor

MOLL_Svel

ROTI_Rrot

MOLL_Pcus

MOLL_Hphy

GAST_Megd

ROTI_Egad

ANNE_Myzo

CHAE_Spdl

ROTI_Mhir

GNAT_Augn

ENTO_PclF

100

100

99

21

47

55

100

100

100

53

100

24

100

14

100100

98

100

19

100

73

100

33

100

65

70

100

81

100

100

100100

100

100

100

100

100

100

59

100100

94

73

100

100

76

100

85

50

100

98

91

100

100

100

100

100

100

100

100

100

73

99

100

94

100

97

18

100

100

100

92

35

100

86

61

77

35

S14

0.3

TARD_HdEd

ARTH_Fcan

GNAT_Trik

ARTH_Isca

NEMA_Rcul

GAST_Dnot

ARTH_Scut

ONYC_Pcap

BRAC_Ttvs

ARTH_Cfin

ARTH_Etai

ARTH_Smar

ONYC_PeriARTH_Ains

NEMM_Nect

NEMA_Lloa

KINO_Eduj

ARTH_Limu

ANNE_Ctel

PRIA_Pcau

MICR_Limn

ONYC_Oope

NEMA_HconNEMA_Celg

PRIA_Meio

NEMA_Tspi

MOLL_Lott

NEMA_Tmur

NEMA_Pred

ARTH_Fmer

TARD_Mtar

ARTH_Dpul

ARTH_Gmar

LORI_Arlc

ARTH_Dmel

NEMA_Ppac

KINO_Pkie

ARTH_Amel

PLAT_Pvit

ONYC_Pept

NEME_Cham

ARTH_Smim

PHOR_Phar

1

1

1

0.67

1

1

1

0.75

0.94

1

1

1

1

0.99

1

1

1

1

1

1

1

1

1

1

1

0.92

1

1

1

1

1

1

0.99

1

0.96

0.61

1

1

1

0.72

1S15

0.3

PRIA_Meio

NEME_Cham

ARTH_Amel

ARTH_Smar

MOLL_Lott

ONYC_Pcap

NEMA_Tmur

ARTH_Dmel

ARTH_Fcan

ARTH_Gmar

ARTH_Isca

PRIA_Pcau

NEMA_Celg

GAST_Dnot

ARTH_Ains

ARTH_Etai

TARD_Mtar

PHOR_Phar

MICR_Limn

ONYC_Oope

ARTH_Fmer

NEMA_Ppac

KINO_Pkie

ANNE_Ctel

GNAT_Trik

KINO_Eduj

ARTH_Cfin

PLAT_Pvit

ARTH_Limu

NEMA_Lloa

NEMA_Rcul

NEMA_Hcon

NEMA_Pred

BRAC_Ttvs

ARTH_Smim

ONYC_Peri

ONYC_Pept

LORI_Arlc

ARTH_Dpul

ARTH_Scut

TARD_HdEd

NEMA_Tspi

1

0.82

1

11

1

11

1

1

1

1

0.97

1

0.99

1

1

1

1

0.96

0.67

1

0.75

1

1

1

1

1

1

1

1

1

1

1

1

1

0.94

1

1

1

S16

0.2

ARTH_Etai

TARD_Mtar

ANNE_Ctel

PRIA_Pcau

TARD_HdEd

ARTH_Smar

NEMA_Celg

ARTH_Dmel

LORI_Arlc

KINO_Eduj

NEME_Cham

ARTH_Limu

NEMA_Tmur

NEMA_Hcon

ARTH_Amel

GAST_Dnot

ARTH_Smim

NEMA_Rcul

BRAC_Ttvs

ARTH_Dpul

ARTH_Scut

NEMA_Tspi

PLAT_Pvit

MICR_Limn

ARTH_Ains

PHOR_Phar

ARTH_Gmar

NEMA_Ppac

ONYC_Pept

ONYC_Pcap

NEMM_Nect

NEMA_Lloa

KINO_Pkie

ARTH_Isca

MOLL_Lott

NEMA_Pred

GNAT_Trik

ARTH_Fmer

ONYC_Peri

ARTH_Cfin

PRIA_Meio

ONYC_Oope

ARTH_Fcan

1

0.95

1

1

0.6

1

1

1

1

1

1

1

1

1

1

1

1

0.94

0.98

1

1

1

1

1

1

0.92

1

0.5

1

0.99

1

1

0.64

1

1

0.93

0.5

1

0.57

S17

0.2

KINO_Eduj

NEMA_Rcul

NEMA_Pred

ARTH_Dpul

TARD_Mtar

PRIA_Meio

ARTH_Dmel

ANNE_Ctel

ARTH_Smim

PHOR_Phar

NEMA_Ppac

ARTH_Etai

NEMA_Lloa

ARTH_Smar

ONYC_Oope

NEMA_Tmur

BRAC_Ttvs

ONYC_Peri

PRIA_Pcau

NEMA_Hcon

TARD_HdEd

ARTH_Fcan

NEMA_Celg

NEMM_Nect

ONYC_Pept

ARTH_Isca

ARTH_FmerARTH_Cfin

ONYC_Pcap

GNAT_Trik

ARTH_Limu

ARTH_Amel

ARTH_Scut

ARTH_Ains

NEMA_Tspi

MICR_Limn

GAST_Dnot

NEME_Cham

MOLL_Lott

ARTH_Gmar

PLAT_Pvit

KINO_Pkie

LORI_Arlc

100

100100

100

96

85

41

100

90

83

35

100

90

77

9996

100

100

95

100

100

100

100

89

100

100

84

74

100

100

100

100

100

99

100

100

50

100

100

100

58

S18

0.5

CNID_Hvul

PORI_Scil

CEPH_Bflo

PORI_Slac

CTEN_Mlei

CNID_Csow

OUTI_Ttra

CNID_Gven

OUTC_Mbre

PORI_Cele

OUTF_Amac

PORI_Pfic

CTEN_Pbac

OUTF_Spun

OUTC_Sros

CNID_Adig

PORI_Psub

OUTI_Apar

BRAC_Ttvs

CRAN_MmusECHI_SpurXENO_XbJC

PORI_Cnuc

OUTI_Falb

PORI_Ocar

CNID_Phyd

PLAC_Tadh

PORI_Scoa

OUTI_Mvar

ONYC_Peri

OUTI_Sart

OUTF_Mver

OUTF_Rall

PORI_Avas

CTEN_Baby

PRIA_Pcau

OUTI_Cowc

PORI_Ccla

PORI_Cvar

ANNE_Ctel

CNID_Smel

PORI_Ifas

PORI_Ccan

CTEN_Vmul

MOLL_Lott

CNID_Nvec

CNID_Atet

PORI_Aque

CTEN_Edun

OUTF_FoxyOUTF_Rsol

1

1

1

1

1

1

1

1

1

1

1

1

1

1

0.59

1

0.98

1

1

0.96

1

1

1

1

1

0.96

1

1

11

1

0.96

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

S19

0.3

CTEN_Baby

PORI_Pfic

PLAC_Tadh

CTEN_Mlei

PORI_Ocar

CNID_Gven

PORI_Aque

OUTF_Mver

OUTF_Amac

CTEN_Pbac

MOLL_LottBRAC_Ttvs

CTEN_Vmul

OUTI_Mvar

CNID_Csow

PORI_Cvar

CNID_PhydCNID_Smel

PORI_Psub

PORI_Slac

ECHI_Spur

PORI_Ccan

OUTC_Mbre

PORI_Ifas

CNID_Atet

OUTI_Ttra

OUTI_Apar

ONYC_Peri

PORI_ScoaPORI_Avas

CEPH_Bflo

CNID_Adig

XENO_XbJC

OUTI_Cowc

CNID_Nvec

PORI_Scil

PORI_Cnuc

OUTC_Sros

OUTF_Rall

CTEN_Edun

PORI_Ccla

OUTF_Foxy

OUTF_Spun

OUTF_Rsol

PORI_Cele

CNID_Hvul

OUTI_Falb

CRAN_Mmus

ANNE_Ctel

OUTI_Sart

PRIA_Pcau

1

1

1

0.92

0.99

1

1

1

1

1 1

1

0.61

1

0.61

1

1

0.8

1

1

1

0.95

1

1

1

0.99

1

1

11

1

0.99

0.53

0.72

1

1

0.79

1

1

11

1

1

0.81

1

1

1

1

S20

0.4

CNID_Adig

OUTC_Mbre

BRAC_Ttvs

PORI_Pfic

CEPH_Bflo

OUTC_Sros

PORI_Cele

PORI_Scil

PLAC_Tadh

PORI_Slac

ANNE_Ctel

PORI_Scoa

PORI_Ocar

PORI_Aque

CTEN_Baby

CNID_Smel

CTEN_EdunCTEN_Vmul

PORI_Cvar

PRIA_Pcau

CNID_Phyd

PORI_Avas

CTEN_Pbac

CRAN_Mmus

CTEN_Mlei

PORI_Psub

CNID_Nvec

PORI_Cnuc

ONYC_Peri

CNID_Hvul

CNID_Csow

CNID_Gven

PORI_Ifas

ECHI_Spur

PORI_Ccla

MOLL_Lott

XENO_XbJC

CNID_Atet

PORI_Ccan

0.5

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

0.55 1

11

1

1

1

1

1

S21

0.2

PORI_Ccan

XENO_XbJC

OUTC_Sros

CEPH_Bflo

PORI_PficPORI_Aque

PORI_Ocar

CTEN_Vmul

PORI_Slac

PRIA_Pcau

CNID_Phyd

PORI_Avas

CTEN_Mlei

PORI_Cvar

PORI_Scil

CRAN_Mmus

PORI_Ccla

CTEN_Pbac

PORI_Cnuc

BRAC_Ttvs

CNID_Atet

PLAC_Tadh

CNID_Smel

ONYC_Peri

PORI_Scoa

CNID_Nvec

ANNE_CtelMOLL_Lott

PORI_Cele

CNID_Hvul

CTEN_Edun

ECHI_Spur

CTEN_Baby

CNID_Csow

CNID_Gven

PORI_Psub

CNID_Adig

OUTC_Mbre

PORI_Ifas

1

11

1

1

0.98

1

1

0.99

1

1

1

1

0.99

1

1

1

0.99

1

1

1

1

0.91

1

1

1

0.57

1

1

1

1

0.88

1

1

1

1

0.85

S22

ONYC_Peri

PORI_Ifas

OUTF_Rall

OUTF_Spun

CRAN_Mmus

OUTF_Rsol

CNID_Atet

PORI_Cvar

PORI_Avas

PORI_Ccla

CNID_Adig

ECHI_Spur

CNID_Csow

PRIA_Pcau

PORI_Psub

OUTI_Mvar

CNID_Hvul

PORI_Cnuc

OUTI_Apar

CTEN_Edun

PORI_Scoa

CNID_Smel

OUTF_Amac

CNID_Nvec

OUTI_Falb

PORI_Cele

CTEN_Mlei

PORI_Aque

PORI_Ocar

XENO_XbJC

OUTF_Foxy

PORI_Scil

PORI_Slac

OUTF_Mver

OUTI_Sart

BRAC_Ttvs

CEPH_Bflo

ANNE_Ctel

CNID_Phyd

PORI_Ccan

CTEN_Baby

OUTC_MbreOUTC_Sros

CTEN_Pbac

CNID_GvenPLAC_Tadh

OUTI_Cowc

MOLL_Lott

PORI_Pfic

CTEN_Vmul

OUTI_Ttra

100

100

100

100

96

100

100

100

100

100

100

100

100

72

100

100

100

100

100

100

97

100

86

100

95

91

100

57

100

100

100

100

100

100

100

100

100

100

100

100

74

100

100

100

10056

100

100

100

S23

MOLL_Lott

PORI_Scil

CEPH_BfloPRIA_Pcau

PORI_Ccan

CTEN_Pbac

CRAN_Mmus

PORI_Cnuc

BRAC_Ttvs

PORI_Cele

ANNE_Ctel

PORI_Avas

XENO_XbJC

CNID_Hvul

CNID_AdigCNID_Nvec

CTEN_Baby

CNID_Csow

PORI_Cvar

PORI_Aque

CTEN_Edun

PORI_Psub

PORI_Ocar

ONYC_Peri

PLAC_TadhCNID_Gven

PORI_Pfic

OUTC_Mbre

PORI_Ccla

OUTC_Sros

CTEN_Vmul

ECHI_Spur

PORI_Slac

CTEN_Mlei

CNID_Smel

CNID_Atet

CNID_Phyd

PORI_Scoa

PORI_Ifas

96

100

80

84

100

100

100

100

100

97

100

79

86

100

100

100

100

100

100

100

100

100

100

100

100

100

100

100

100

100

100

80

100

100

100

100

100

S24

NON-BILATERIACtenophora [5] Beroe abyssicola CTEN_Baby Transcriptome (Illumina) SRR777787

Euplokamis dunlapae CTEN_Edun Transcriptome (Illumina) SRR777663Mnemiopsis leidyi CTEN_Mlei Genome PRJNA64405Pleurobrachia bachei CTEN_Pbac Genome PRJNA213480Vallicula multiformis CTEN_Vmul Transcriptome (Illumina) SRR786489

Porifera [14] Amphimedon queenslandica PORI_Aque Genome PRJNA66531Aphrocallistes vastus PORI_Avas† Transcriptome (Illumina) SRR1068281Chondrilla caribensis PORI_Cnuc Transcriptome (Illumina) https://dataverse.harvard.edu/dataverse/spotranscriptomesClathrina coriacea PORI_Ccla† Transcriptome (Illumina) SRR3417192Cliona varians PORI_Cvar Transcriptome (Illumina) SRR1391011Corticium candelabrum PORI_Ccan Transcriptome (Illumina) SRR504694Crella elegans PORI_Cele Transcriptome (Illumina) SRR648671Ircinia fasciculata PORI_Ifas Transcriptome (Illumina) https://dataverse.harvard.edu/dataverse/spotranscriptomesOscarella carmela PORI_Ocar Genome SRX386229Petrosia ficiformis PORI_Pfic Transcriptome (Illumina) SRR504688Pseudospongosorites suberitoides PORI_Psub Transcriptome (Illumina) https://dataverse.harvard.edu/dataverse/spotranscriptomesSpongilla lacustris PORI_Slac Transcriptome (Illumina) SRR1168575Sycon ciliatum PORI_Scil Transcriptome (Illumina) ERR466762Sycon coactum PORI_Scoa Transcriptome (Illumina) SRR504689,SRR504690

Placozoa [1] Trichoplax adhaerens PLAC_Tadh Genome PRJNA12874Cnidaria [8] Abylopsis tetragona CNID_Atet Transcriptome (Illumina) SRR871525

Acropora digitifera CNID_Adig Genome PRJDA67425Craspedacusta sowerbyi CNID_Csow Transcriptome (Illumina) SRR923472Gorgonia ventalina CNID_Gven Transcriptome (Illumina) SRR935083Hydra vulgaris CNID_Hvul Genome PRJNA31231Nematostella vectensis CNID_Nvec Genome PRJNA19965Polypodium hydriforme CNID_Phyd Transcriptome (Illumina) SRR1336770Stomolophus meleagris CNID_Smel Transcriptome (Illumina) SRR1168418

XENACOELOMORPHAAcoelomorpha [8] Convolutriloba macropyga ACOE_Conv Transcriptome (Illumina) SRR2681679

Diopisthoporus longitubus ACOE_Diol Transcriptome (Illumina) SRR3105704Diopisthoporus gymnopharyngeus ACOE_Diop Transcriptome (Illumina) SRR3105703Eumecynostomum macrobursalium ACOE_Eumc† Transcriptome (Illumina) SRR3105705Hofstenia miamia ACOE_Hmia Transcriptome (Illumina) SRR1208932Isodiametra pulchra ACOE_Isop Transcriptome (Illumina) SRR2681926Neochildia fusca ACOE_Nfus Transcriptome (Illumina) SRR8617822Symsagittifera roscoffensis ACOE_Sros Transcriptome (Illumina) SRR827579

Nemertodermatida [4] Ascoparia sp. BV-2015 NEMO_Asco Transcriptome (Illumina) SRR2682154Flagellophora sp. Bocas NEMO_Flag† Transcriptome (Illumina) SRR8641368Meara stichopi NEMO_Mers Transcriptome (Illumina) SRR2681155Nemertoderma westbladi NEMO_Nemw Transcriptome (Illumina) SRR2682004

Xenoturbellida [1] Xenoturbella bocki XENO_Xboc† Transcriptome (Illumina) SRR8638116Xenoturbella bocki XENO_XbJC Transcriptome (Illumina) SRR2681987

DEUTEROSTOMIAEchinodermata [11] Apostichopus japonicus ECHI_Ajap Transcriptome (Illumina) SRR1185973

Echinaster spinulosus ECHI_Espi Transcriptome (Illumina) SRR1139455

Eucidaris tribuloides ECHI_Etri Transcriptome (Illumina) SRR1138704Holothuria glaberrima ECHI_Hgla Transcriptome (Illumina) SRR490924Lytechinus variegatus ECHI_Lvar Genome SRX056025Ophiocoma echinata ECHI_Oech Transcriptome (Illumina) SRR1138707Ophioderma longicauda ECHI_Olon Transcriptome (Illumina) SRR1325052Oxycomanthus japonicus ECHI_Ojap Transcriptome (Illumina) SRR1138706Patiria miniata ECHI_Pmin Genome SRX096949Sclerodactyla briareus ECHI_Sbria Transcriptome (Il

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Supplemental materials and methods...Aug 31, 2019 · Supplemental materials and methods Sample collection, preservation, and RNA extraction Specimens were preserved during field