SYRINGE SELECTION - Greyhound Chromatography · 1. Syringe Types Key to selecting the right syringe for your application is to identify your sample type and determine the smallest

Complete Guide to Selecting the RightHamilton GASTIGHT®, MICROLITERTM, andSpecialty Syringe for Your Application

SYRINGE SELECTION

Introduction 3

SyringeSchematics 4

SelectingtheRightSyringeforYourApplication–Step-by-StepGuide 4 HamiltonSyringeSelectionWorksheet 5 ExampleofCompletedHamiltonSyringeSelectionWorksheet 6

1.SyringeTypes 7

2.SyringeSeries 8 SyringeTypestoSeriesChart 8

3.SeriesDescriptions 9 MICROLITERSyringes(0.05µL-500µL) 9 600Series(0.25µL-5µL) 9 700Series(0.5µL-500µL) 9 800Series(0.5µL-250µL) 10 900Series(0.5µL-10µL) 10 7000Series(0.05µL-5µL) 10 GASTIGHTSyringes(1µL-2L) 11 1000Series(100µL-100mL) 11 1700Series(1µL-500µL) 11 1800Series(1µL-250µL) 12 SuperSyringe(50mL-2L) 12

4.Terminations 13 N,CementedNeedle 13 SN,SpecialCementedNeedle 13 LTN,LuerTipCementedNeedle 13 LTSN,LuerTipSpecialCementedNeedle 13 LT,LuerTip 13 RN,RemovalNeedle 14 KH,KnurledHub 14 TLL,PTFE Luer Lock 14 SL,SampleLock™ 14

5.NeedleSelection 15 StandardNeedles 15 CustomNeedles 15 CustomNeedleHubs 15 PointStylesandApplications 16 NeedlePointStyleChart 16 GaugeandLength 17 GaugeIndexTable 17

Accessories,ReplacementParts,andServices 18

AdditionalTechnicalInformation 19

TechnicalSupport 19

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Table of Contents

Hamiltonsyringesarethefinestqualityprecisionfluidmeasuringdevicesavailable.WeofferthemostcompleteselectionofsyringesonthemarketforuseinapplicationsthatrangefromsimpleliquidtransferstoanimalinjectionstocomplexGCandHPLCchromatographicinjections.

Inthisguide,alltheinformationneededtoselecttherightsyringeformanualapplicationsispresented.Inaddition,Hamiltonoffersacompletelineofsyringesforusewithinstrumentationsuchasautosamplersandsyringepumps,includingreplacementsyringesforpumpsproducedbyothermanufacturers.TopqualitymaterialsandskilledworkmanshipensurethatHamiltonsyringesconsistentlydeliverthehighestpossibleperformanceforreliableanalyses.

Oursyringesareaccuratetowithin±1%ofnominalvolumewithaprecisionof1%at80%ofthetotalvolume.ThefluidpathofaHamiltonsyringeisdesignedtobechemicallyinertwithstainlesssteel,borosilicatetypeIglass,andPTFE usedformostsyringes.N.I.S.T.traceablecertificationisavailable,asanadditionalservice,forthemajorityofthesyringesinourproductline.

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Introduction

AllHamiltonproductsareunconditionallyguaranteedtobefreeofdefectsinmaterialsandworkmanshipforoneyear(12months)fromdateofpurchase.TheHamiltonCompanyQualitySystemisISO9001-2000Certified.SyringesandneedlesmanufacturedbyHamiltonCompanyareintendedforscientificresearchandlaboratoryuseonlyandarenotintendedforhumaninvivouse.ConsultourpublishedspecificationstodeterminethematerialcompatibilityofHamiltonproductswithyourapplication.

Hamiltoncontinuouslyresearchesnewmaterialsandmethodstoimproveform,fit,andfunctionofoursyringes,soyoucanbeconfidentthatwhenyoubuyfromHamiltonyouareonthecuttingedge.Forthelatestinformationonnewproducts,detailedproductandpartdescriptions,publishedspecifications,andourGuide to Maintaining and Using Hamilton Syringes,pleasevisitwww.hamiltoncompany.com

FlangePlunger

Plunger Tip (PTFE)

O-Ring

Needle

BarrelTermination

Volume Markings

Reference Drawing(Not to Scale)

MICROLITERsyringeshaveastainlesssteelplungerwhichisindividuallyhand-fittedtoitsmatchingglassbarrel.Thehand-fittingprocessisfinelycontrolledtocreatealiquid-tightsealbetweenthebarrelandtheplunger.PlungersforMICROLITERsyringescannotbeinterchangedorreplacedifdamaged.

ExamplesofHamiltonMICROLITERandGASTIGHTPrecisionSyringes

For liquids

For gas and liquids

700 Series MICROLITER SyringeCemented Needle (N)Needle Point Style 2

1700 Series GASTIGHT SyringeCemented Needle (N)Needle Point Style 2


Syringe Schematics

GASTIGHTsyringeshaveaprecisionmachinedPTFEplungertipwhichprovidesatightsealforbothliquidsandgases.ReplacementplungerassembliesareavailableformostGASTIGHTsyringes.

Hamiltonofferssevencoreserieswithawiderangeofdeliveryvolumes,terminationtypes,needlegauges,andneedlepointstylesplusnumerousspecialitysyringes.Hamilton’ssyringeseriesaregroupedbysampletype,application,andvolumerange.Oursyringesarefurthersupportedbyanarrayofaccessoriestoimprovedurabilityandreproducibility.

Useourfive-stepguideandworksheettochoosetheidealsyringeforyourapplication.ThenextpageisablankHamiltonSyringeSelectionWorksheet.Theblank

Selecting the Right Syringe for Your Application Step-by-Step Guide

worksheetisfollowedbyasampleofacompletedworksheet.Instructionsforusingtheworksheetareincludedontheform.Oncetheworksheetiscompleted,youcanfullydescribethesyringeneededforyourapplication.

Note:youmaywanttomakecopiesoftheblankworksheetforfutureuseoryoucandownloadacopybyvisitingwww.hamiltoncompany.com


Hamilton Syringe Selection Worksheet

Tousethisworksheet,firstdefinethesampletypeandrequireddispensingvolume.Next,readeachstep,andforadditionaldetailsgototheappropriatereferencesectioninthisguide.Choosethefeatureneededand,enteryourselectionontheworksheet.Oncompletion,this

TheRightHamiltonSyringeforMyApplicationis:

Type Series Volume Termination Needle

StepstoChoosingaSyringe

1. SeeSyringe Types, pg. 7,todecidewhetheraGASTIGHToraMICROLITERsyringeisbestfortheapplication.Note:GASTIGHTsyringesarerecommendedforviscoussamples.WithMICROLITERsyringes,itispossibleforairtoslippastaplungerasavacuumiscreated.

2. UsetheSyringe to Series Chart, pg. 8,todeterminetheavailablesyringeseriesinthevolumerangeneeded.

3. SeetheSeries Descriptions, pg. 9,todeterminethemostappropriateseries,andtheavailablevolumesandterminationsforthatseries.

4. SeeTerminations, pg. 13, todeterminethemostsuitabletermination.

5. SeeNeedle Selection, pg. 15,todeterminethemostappropriateneedleifaterminationorapplicationrequirestheselectionofaspecificneedle.

a.StandardNeedles.MostHamiltonsyringesareavailablewith2in.needlesofanappropriategaugewitheitherPointStyle2(sharppoint)orPointStyle3(bluntpoint).

b.CustomNeedles.Iftheapplicationrequiresanon-standardlength,gaugeorpointstyle,thenHamiltonoffersseveralcustomoptions.SeeCustom Needles, pg. 15,todeterminetheappropriateneedleforanyapplication.

YourSelection

1. GASTIGHT______________MICROLITER____________

YourdispensingvolumeinµL_____________

2. Seriesavailable__________________________

_________________________________________

3. Seriesselected___________________________

Volumeselected_________________________

Terminationsavailable____________________

_________________________________________

4. Terminationsselected____________________

5. StandardNeedle PointStyle2_____________

PointStyle3_____________

CustomNeedle Gauge_______________________

Lengthininches_______________

PointStyle____________________

NeedleHub___________________

worksheetwillfullydescribetheHamiltonsyringeneededforyourapplication.Visitwww.hamiltoncompany.comtoidentifythepartnumberorcall1-888-525-2123forHamiltonCustomerServiceassistance.CallyourlocaldealerforassistanceoutsidetheU.S.

YourSelection

1. GASTIGHT______________MICROLITER____________

YourdispensingvolumeinµL_____________

2. Seriesavailable__________________________

_________________________________________

3. Seriesselected___________________________

Volumeselected_________________________

Terminationsavailable____________________

_________________________________________

4. Terminationsselected____________________

5. StandardNeedle PointStyle2_____________

PointStyle3_____________

TheRightHamiltonSyringeforMyApplicationis:

Type Series Volume Termination Needle

In this example, Jane needs a syringe that is capable of doing nine rapid 10 µL injections of a blood serum solution for a nitric oxide study using GC analysis.

Jane calls Hamilton Customer Service and orders a GASTIGHT 1800 series 10 µL syringe with a RN hub and a standard sharp needle (Point Style 2).

X

10 µL

1700 and 1800

1800

N and RN

10 µL

RN

X

1800 10 µL RNGASTIGHT Std. Point Style 2


Example of Completed Worksheet

StepstoChoosingaSyringe

1. SeeSyringe Types, pg. 7,todecidewhetheraGASTIGHToraMICROLITERsyringeisbestfortheapplication.Jane’s sample is a liquid, but it is viscous and contains numerous dissolved components that over time could cause a fitted plunger to freeze, so she elects to use a GASTIGHT syringe.

2. UsetheSyringe to Series Chart, pg. 8,todeterminetheavailablesyringeseriesinthevolumerangeneeded.Using the chart, she determines that syringes in the 1700 and 1800 series are available in her volume range.

3. SeetheSeries Descriptions, pg. 9,todeterminethemostappropriateseries,andtheavailablevolumesandterminationsforthatseries.From the descriptions, she determines that although the 1700 series would work, the 1800 series has a supported plunger which eliminates the chance of bending the fragile plunger during the nine rapid injections. She selects the appropriate volume and lists the terminations available for the 1800 series syringes.

4. SeeTerminations, pg. 13,todeterminethemostsuitabletermination. She sees that the 1800 series syringes are available with either Cemented Needle or Removable Needle terminations. She determines that a Removable Needle is the most appropriate termination because if her serum sample clogs the needle she can replace the needle and not the whole syringe.

5. SeeNeedle Selection, pg. 15,todeterminethemostappropriateneedleifaterminationorapplicationrequirestheselectionofaspecificneedle.Since a standard needle is suitable for her application, Jane does not need to supply information for a custom needle.


1. Syringe Types

Keytoselectingtherightsyringeforyourapplicationistoidentifyyoursampletypeanddeterminethesmallestvolumetobedispensedorinjected.Hamiltonofferstwotypesofsyringes,GASTIGHTandMICROLITER,whichdifferinthedesignoftheplunger.

GASTIGHT Syringes for Gases and Liquids

GASTIGHTsyringeshaveaprecision-machinedPTFEplungertipwhichcreatesaleak-freeseal.Withthetightfit,thetipessentiallywipestheinteriorofthesyringebarrelfreeofsample.Thisfeatureisparticularlyusefulwithheterogeneoussamplesasitreducesthechancethatadepositwilloccurandcausetheplungertofreeze.

TheGASTIGHTseriesstillrequirescarefulandregularcleaningtominimizedepositsontheglasswhichmightscorethesoftPTFE plungertipandresultinginaleak.ReplacementplungerassembliesareavailableforGASTIGHTsyringes.However,areplacementplungershouldnotbeputintoabarrelwhichstillcontainsdepositsbecausethenewplungertipislikelytobescoredbythedepositafteronlyafewstrokes.Itisimportanttorememberthatovertime,theincreasedfrictioncreatedbythetightsealmaycausethePTFE tiptowearoutandtheplungerwillhavetobereplaced.

MICROLITER Syringes for Liquids

MICROLITERsyringesincorporateahand-fittedstainlesssteelplungerwithafinelyboredsyringebarrel.Thesesyringesareidealforhomogenoussamplesthatarenotpronetoprecipitationorbondingwithglass.

Undertheproperconditions,plungerwearisminimalandthelifeofaMICROLITERsyringeisalmostunlimited.However,whenusingheterogeneoussolutionswithaMICROLITERsyringe,theusermustbeespeciallydiligentaboutcleaningthesyringeaftereachuse.Formoreinformation,seeourGuide to Maintaining and Using Hamilton Syringes.

Insomecases,evendiligentcleaningisnotsufficientandthebarrelwillbecomesoiled.Thedepositsontheglasswillcompromisethetighttolerancesbetweentheglassandtheplungerresultinginafrozenplunger.PlungersforMICROLITERsyringescannotbeinterchangedorreplacedifdamaged.Forheterogeneoussolutions,aGASTIGHTsyringeisthebestoption.

TheHamiltonSyringeTypetoSeriesChart

Forgassamplesandheterogeneousliquids,therearefourGASTIGHTsyringeseriesfromwhichtochoose.Forhomogeneousliquidsamples,therearefiveMICROLITERsyringeseries.GiventhevarietyofsyringeseriesavailablewithMICROLITERandGASTIGHTplungers,aneasywaytonarrowdownthelistofseriesistousedelivery(ordispensing)volumeasadiscriminatingfactor.Forthemostaccuratedispensesalwayschooseasyringethathasanominalvolumeasclosetothedispensevolumeaspossible.

Syringe Type

GASTIGHTGas/Liquid

MICROLITERLiquid only

0.05 µL - 0.25 µL

0.25 µL - 0.5 µL

0.5 µL - 5 µL

5 µL - 10 µL

10 µL - 250 µL

250 µL - 500 µL

1 µL - 100 µL

100 µL - 250 µL

250 µL - 500 µL

500 µL - 50 mL

50 mL - 100 mL

100 mL - 2L

1000 Series and Super Syringe

600, 700, 800, 900, 7000 Series

1700, 1800 Series

1000, 1700, 1800 Series

1000, 1700 Series

1000 Series

Super Syringe

7000 Series

600 Series

700, 800 Series

700 Series

700, 800, 900 Series

Delivery Volume Syringe Series


2. Syringe Series

Foraccuracyandprecision,thesmallestdispensingvolumeforagivensyringeshouldbegreaterthanorequalto10%ofitstotalcapacity.Forexample,thesmallestdispensingvolumerecommendedfora10µLsyringeis1µL.ThefollowingSyringeTypetoSeriesChartshowsthevolumesthateachseriesiscapableofdispensingwithin10-100%ofthesyringes’nominalvolume.

CompletedescriptionsforHamiltonsyringeseriesusedformanualapplicationsaregivenbelow.Thedescriptionsincludeproductfeaturesandtypicalapplicationsalongwiththevolumes,terminations,andaccessoriesavailableforeachseries.Forsomevolumes,severalseriesmayincludesyringeswithequivalentvolumerangesandsimilarterminations.


3. Series Descriptions

Recommended Use: The 600 series is great for animal injections because one hand is free for manipulating the animal.Volumes: 2.5 µL and 5 µLTerminations: Removable Needle (RN)Accessories: Syringe Guide and Reproducibility Adapter

Recommended Use: The 700 series is used for manual and automated GC and HPLC injections. Also, it is used for everyday applications that require accurate measuring and dispensing of liquid.Volumes: 5 µL, 10 µL, 25 µL, 50 µL, 100 µL, 250 µL, and 500 µLTerminations: Cemented Needle (N), Special Cemented Needle (SN), Removable Needle (RN) and Luer Tip (LT)Accessories: PB600 Repeating Dispenser, Reproducibility Adapter, Syringe Guide, and Digital Syringe

MICROLITER Syringes (0.05 µL - 500 µL Delivery Volume)

Thistypeofsyringeisforusewithliquidsandincorporatesastainlesssteelplungerthatisindividuallyfittedtoitsmatchingsyringebarrel.Thesemicro-volumesyringeshaveaveryclosetolerancebetweentheplungerandthebarrelwhichcreatesaliquid-tightsealwithoutpartssuchasO-ringsthateventuallywearout.

600 Series (0.25 µL - 5 µL Delivery Volume)

The600seriesarerobustsyringesconsistingoftwodistinctparts.Thebottomhalfofthebarrelaccuratelymeasurestheliquidsampleandthetophalfsupportstheplunger.Inaddition,thetopsectionoftheplungeristhickertofurtherreducetheriskofbendingtheplunger.Thesesyringesrequirehalfofthestandardstrokelengthmakingthemidealforone-handedoperation.Theplungersandbarrelsarenotinterchangeableorreplaceable.


The700seriesistheoriginalHamiltonsyringe.Itwasdesignedtosolvethegeneralliquidhandlingrequirementsofmanufacturingandresearchlaboratoriesandremainstheindustrystandard.Theplungersandbarrelsarenotinterchangeableorreplaceable.

Barrel Assembly

Plunger AssemblyReference Drawing

(Not to Scale)

RN Nut RN FerruleRN Needle


LT

N

RN Nut RN Needle Plunger Assembly

Barrel Assembly

RN Ferrule

RN



The800serieshasthesameliquidhandlingcapabilitiesasthe700series,butwiththeadditionofanaluminumsyringeholderdesignedtoeliminatethepossibilityofplungerdamage.Thesyringeholderscrewsontotheglassbarrel.Atwo-pieceextendedplungerfurthereliminatestheriskofbreakage.Africtionscrewatthetopofthebarrelcanbeadjustedtogivetheusersomecontroloverdispensingspeedandpreventtheaccidentalremovaloftheplunger.Inaddition,thesyringeholdercanbefittedwithdifferentbarrel/plungerassembliestocoverarangeofvolumes.


The900serieshasthesameliquidhandlingcapabilitiesasthe800seriesbutisamoreeconomicalversion.The900seriesdoesnothaveanyreplaceablepartsmakingtheinitialcostlessthanthe800series.

Recommended Use: This syringe is intended for applications where the plunger on a 700 series syringe might be bent. Excellent for novice users.Volumes: 5 µL, 10 µL, 25 µL, 50 µL, 100 µL, and 250 µLTerminations: Cemented Needle (N), Special Cemented Needle (SN), and Removable Needle (RN)Accessories: Reproducibility Adapter

Recommended Use: This syringe is perfect when a robust syringe is desired and budget is a consideration.Volumes: 5 µL and 10 µLTerminations: Cemented Needle (N), Special Cemented Needle (SN), and Removable Needle (RN)Accessories: Reproducibility Adapter


The7000seriesemploysaplungerwireinsidetheneedletoaccuratelydispenseultra-lowvolumes.Theneedleisboredtoextremelyaccuratetolerancestoaccommodatetheplungerwire.Withtheplungerinsidetheneedle,thestandarddeadvolumeinsidetheneedleiseliminated.

Recommended Use: The 7000 series is used for manual and automated GC and HPLC injections. Also, it is used for everyday applications that require ultra-small measurements of liquid.Volumes: 0.5 µL, 1 µL, 2 µL, and 5 µLTerminations: Knurled Hub (KH)Accessories: Reproducibility Adapter, Syringe Guide, Digital Syringe, and Syringe Cleaner


Plunger Assembly

Friction Ring (PTFE) Friction Screw

RN Nut RN Needle RN Ferrule

Syringe Holder

NBarrel Assembly

RN

Holder Assembly

Plunger Stem


N

RN Nut

RN Needle

Plunger Assembly

Barrel Assembly

RN Ferrule

RN


Barrel Assembly

Wire and Plunger Assembly

KH Needle with FerruleKnurled Hub Nut


RN Nut

RN Needle

RN Ferrule

LT

LTN

TLL

Plunger Assembly

Barrel Assembly

RN

(Typical SyringeTerminations)

6-32 UNCPlunger Tip (PTFE)

O-Ring

Recommended Use: The 1000 series is ideal for manual and automated HPLC injections. This syringe is commonly used in syringe pumps and other liquid handling instrumentation. Volumes: 1 mL, 1.25 mL, 2.5 mL, 5 mL, 10 mL, 25 mL, 50 mL, and 100 mLTerminations: Luer Tip Cemented Needle (LTN), Luer Tip Special Cemented Needle (LTSN), Luer Tip (LT), Removable Needle (RN), SampleLock (SL), and PTFE Luer Lock (TLL)Accessories: PB600 Repeating Dispenser for volumes(1 to 2.5 mL)


Recommended Use: The 1700 series is excellent for manual and automated GC and HPLC injections. This syringe is commonly used in syringe pumps and other liquid handling instrumentation.Volumes: 10 µL, 25 µL, 50 µL, 100 µL, 250 µL, and 500 µLTerminations: Cemented Needle (N), Special Cemented Needle (SN), Luer Tip Cemented Needle (LTN), Luer Tip Special Cemented Needle (LTSN), Luer Tip (LT), Removable Needle (RN), SampleLock (SL), and PTFE Luer Lock (TLL)Accessories: PB600 Repeating Dispenser, Reproducibility Adapter, Syringe Guide, and Digital Syringe

1700 Series (1 µL - 500 µL Delivery Volume)

ThisistheGASTIGHTversionoftheoriginalHamilton700seriessyringe.Itwasdesignedtomeetthelowvolumegasorliquidhandlingneedsofresearchandmanufacturinglaboratories.

GASTIGHT Syringes (1 µL - 100 mL Delivery Volume)

Thesesyringescanbeusedwithbothgasesandliquids.Theprecision-machinedPTFEplungertipcreatesaleak-freeseal.Theplungersarereplaceable.

1000 Series (100 µL - 100 mL Delivery Volume)

The1000seriesisamid-volumesolutionforallliquidandgashandlingneeds.


Plunger AssemblyO-RingRN Nut

RN Needle

RN Ferrule

LT

N

Barrel Assembly

RN

LTN

TLL

(Typical SyringeTerminations)

Plunger Tip (PTFE)


1800 Series (1 µL - 250 µL Delivery Volume)

The1800serieswasdesignedtoeliminatethepossibilityofplungerdamage.Ithasthesameliquidhandlingcapabilitiesasthe1700seriesbuttheextendedplungereliminatesbreakageandallowstheusersomecontroloverthedispensespeed.

Super Syringe (50 mL - 2L Delivery Volume)

SuperSyringesweredesignedprimarilyforairsampling,preparinggasstandards,calibratingreservoirs,andpneumographs.TheSuperSyringeistheonlyHamiltonsyringewithanacrylicbarrel,whichmakesthissyringeslightlylesschemicallyresistant.

Recommended Use: This syringe is intended for applications where the plunger on a 1700 series syringe might be bent.Volumes: 10 µL, 25 µL, 50 µL, 100 µL, and 250 µLTerminations: Cemented Needle (N) and Removable Needle (RN)Accessories: Reproducibility Adapter

Recommended Use: This syringe is intended for sampling or transfer of gases.Volumes: 0.5L, 1L, 1.5L, and 2LTerminations: PTFE Luer Lock (TLL) and Tracheal Adapter (accepts 5/8 inch I.D. flexible tubing)Accessories: None

Super Syringes - Specialty Syringes for Gases


Plunger AssemblyPlunger Tip (PTFE)

H

older Assembly


N

Barrel Assembly

RN

Plunger StemFriction ScrewSyringe Holder Friction Ring (PTFE)


HAMILTON CO., RENO, NEVADA

100 200 300 400 500 ml

Super Syringe Barrel

Plunger Assembly

MODEL S-500 MADE IN USA

N, Cemented Needle For low volume syringes

Theneedlesarecementedintotheglasssyringebarrelatapointcorrespondingtothezerograduationmark.Withthistermination,deadvolumeislimitedtotheinternalvolumeoftheneedle.Notautoclavable.Needlegaugeisdeterminedbythesyringevolumeandarenotuser-selectable.Foravailableneedlegauges,seewww.hamiltoncompany.com

SN, Special Cemented Needle For low volume syringes

ThespecialneedleterminationsarethesameastheCementedNeedleterminationsexcepttheyallowforavarietyofuser-definedgauges,lengths,andpointstylestobeattached.

LTN, Luer Tip Cemented Needle For mid volume syringes

Theneedlesarecementedintotheglasssyringebarrelatapointcorrespondingtothezerograduationmark.Withthistermination,deadvolumeislimitedtotheinternalvolumeoftheneedle.Notautoclavable.Needlegaugeisdeterminedbythesyringevolumeandarenotuser-selectable.Foravailableneedlegauges,seewww.hamiltoncompany.com

LTSN, Luer Tip Special Cemented NeedleFor mid volume syringes

ThespecialneedleterminationsarethesameastheLuerTipCementedNeedleterminationsexcepttheyallowforavarietyofuser-definedgauges,lengths,andpointstylestobeattached.

LT, Luer Tip

Theneedlesareremovableandfitoveragroundglasshubwhichistaperedintheshapeofamaleluer.TheLTterminationwillacceptmosthypodermicneedlesbutwasdesignedspecificallyforusewithHamiltonKel-Fneedles.Thisterminationincreasesthedeadvolumeinthesyringe,whichmaynotbeappropriateforsomeapplications.Autoclavablewhendisassembled.

Terminationsarelocatedattheendofthesyringebarrelandfunctionastheinterfacebetweenthesyringeanditsmatingconnectionsuchastheneedle.Terminationsareofferedinanumberofdifferentneedleandconnection


4. Terminations

configurationstoaccommodateabroadrangeofapplications.Belowisalistingofthemostpopularsyringeterminations.Foracompleteoverview,pleasevisitwww.hamiltoncompany.com

SN

N

LTN

LTSN

LT

RN, Removable Needle

TheneedlesareremovableandareaHamilton-specificdesign.Thedesignallowstheneedlestoseatpreciselyatthezerograduationmarkofthesyringe.Userscanselecttheneedlegauge,length,andpointstyletooptimizethesyringeforcustomapplications.Additionally,thisterminationallowsforaremovableneedlewithoutincreasingthedeadvolumeofthesyringeandisidealwhenthereisariskoftheneedleclogging.Autoclavablewhendisassembled.Repeatedautoclavingwillshortensyringelife.Formoreinformation,seeourGuide to Maintaining and Using Hamilton Syringes.

KH, Knurled Hub

Theknurledhubisusedexclusivelyon7000Seriessyringes.Thehubhandlesupto6000psigmaximuminjectionpressure.Theneedleisremovablebutwithalimitednumberofgaugesavailablebecausetheplungerisfittedinsidetheneedle.Autoclavablewhendisassembled.Repeatedautoclavingwillshortensyringelife.Formoreinformation,seeourGuide to Maintaining and Using Hamilton Syringes.

TLL, PTFE Luer Lock

ThisterminationhasaPTFE,maleLuertaperwithnickel-platedbrasslockinghubforusewithKel-Fneedles,metalhubneedles,anduniversalconnectors.Also,theTLLisusedwithHamiltondiluters/dispensers,OEMapplications,andmanualoperations.Autoclavablewhendisassembled,excepton25mLandgreatersyringes.Repeatedautoclavingwillshortensyringelife.Formoreinformation,seeourGuide to Maintaining and Using Hamilton Syringes.

SL, SampleLock™

ThesamplelockincorporatesanOn/OffsyringevalvewithRNneedle.Thisterminationisusedforheadspace,environmentalsamplecollectionandstorage,pre-pressurizationofgaseoussamplesforGCanalysis,andsamplespiking.Notautoclavable.


Reference Drawing(Not to Scale)RN NeedleRN Nut

SL

TLL

KH

KH Needlewith FerruleKnurled Hub Nut


RN

Standard Needles

Formostsyringes,Hamiltonhasalreadyselectedthemostcommongauge(22or26),pointstyle(2or3)andlength(2inches)fortheaverageapplication.Throughoutourwebsiteandcatalogs,theneedlesaredesignatedby(Gauge/Length(inches)/PointStyle),e.g.,(22s/2/2).ReviewtheNeedlePointStyleCharttodetermineifastandardpointstyleissuitableorifacustomneedleisnecessaryforyourapplication.

NeedlepointstylesrangefrombluntpointsforHPLCinjectionstoconicalpointsforpenetratingvinylandplastics.Withmostsyringes,Hamiltonprovidesastandard2-inchneedleofanappropriategaugeandpointstyle.However,ifyourprojectrequiresanon-standardneedlegaugeorpointstyle,Hamiltonoffersawidevarietyofcustomoptions.


5. Needle Selection

Custom Needles

Forourcustomneedles,theuser-definableparametersareindicatedbyasterisklike(22s/*/*).Reviewthefollowingsectionstofullydefinethecustomneedlethatisrequired.Therearelimitstothegauge,length,andpointstylecombinationsthatareavailablesocontactHamiltonCustomerServiceat1-888-525-2123forassistance.ContactyourlocaldealerforassistanceoutsidetheU.S.

Custom Needle Hubs

Chooseaneedlehubbasedonthesyringevolumeandthecompatibleterminationforthespecificsyringeyouhaveselectedforyourapplication.

SN, Special Cemented Needle: These are cemented needle syringes that allow for a user-defined needles to be attached from the factory.

LTSN, Luer Tip Special Needle: These are Luer Tip Cemented Needles that allow for user-defined needles to be attached from the factory.

N, Metal Hub Luer Lock Needle: These needles are designed for use with the TLL syringe termination and are available in a variety of gauges between 33 and 10.

KF, Kel-F Hub Luer Lock Needle: These needles are designed for use with the LT and TLL syringe terminations and are available in a variety of gauges between 31 and 10.

Small Hub Removable Needle: These needles are designed for use with the RN syringe termination on syringes with a nominal volume less than or equal to 100 µL. The needles are available in a variety of gauges between 33 and 22. Note: Remove the original needle and PTFE ferrule from the RN termination before inserting the replacement needle assembly.

Large Hub Removable Needle: These needles are designed for use with the RN syringe termination on syringes with a nominal volume greater than or equal to 250 µL. The needles are available in a variety of gauges between 26s and 22. Note: Remove the original needle and PTFEferrule from the RN termination before inserting the replacement needle assembly.

KF

RN NeedleRN Nut Small Hub

RN NeedleRN Nut Large Hub

SN

LTSN

N

Point Styles and Applications

Thecorrectpointstylevariesdependingontheintendedapplication.Belowareafewexamplesofcommonapplications.

AnimalInjections:PointStyle4witha45ºangleallowsforthemostaccuratetargetingofaspecificbiologicalstructure.

ManualHPLCInjections:Mostmanualinjectionvalvesaredesignedtobeusedwitha22gaugePointStyle3needle.

ManualGCInjections:HistoricallyaPointStyle2wasrequiredtoachieveefficientseptumpenetrationwithminimizedcoring.WithHamilton’snewlineofGCseptathereisevidencethatindicatesthePointStyleASwillconsistentlyextendseptalifebyasmuchas10-fold.

DrawingSample:Anypointstylewillefficientlydrawasample,butwhenitisimportanttogetthelastdropoutofavialusePointStyle3orAStoreachallthewaytothebottom.


NeedlePointStyleChartPoint Style 2 Sharp,beveled,curved,non-coringneedlepointrecommended

forseptumpenetration.Availablegauges:33-10.

Point Style 3 BluntneedlepointforusewithHPLCinjectionvalvesandforsamplepipetting.Availablegauges:33-10.

Point Style 4 Standard12°beveledneedlepointisrecommendedforlifescienceapplications.Availablegauges:33-10.

Specialpointstylessuchas30°,45°,oranyotherangleareavailableuponrequest.

Point Style 5 Conicalneedlewithsideportforpenetrationofsepta,thin-gaugedvinylsandplasticswithoutcoring.Availablegauges:26-10.

Point Style AS Specialconicalstyleneedlepointusedonautosamplersyringesthenon-coringneedlepointisrecommendedforseptumpenetration.Availablegauges:26-22.

30º

45º

Gauge inch mm* inch mm* inch mm* µL/inch

33 .0080-.0085 0.21 .0035-.0050 0.11 0.002 0.05 0.20

32 .0090-.0095 0.24 .0035-.0050 0.11 0.002 0.05 0.20

31 .0100-.0105 0.26 .0045-.0060 0.13 0.0025 0.06 0.34

30 .0120-.0125 0.31 .0055-.0070 0.16 0.003 0.08 0.45

28 .0140-.0145 0.36 .0065-.0080 0.18 0.0035 0.09 0.63

27 .0160-.0165 0.41 .0075-.0090 0.21 0.004 0.10 0.80

26s .0184-.0189 0.47 .0045-.0055 0.13 0.007 0.18 0.26

26 .0180-.0185 0.46 .0095-.0110 0.26 0.004 0.10 1.25

25 .0200-.0205 0.51 .0095-.0110 0.26 0.005 0.13 1.25

24 .0220-.0225 0.57 .0115-.0130 0.31 0.005 0.13 1.80

23 .0250-.0225 0.64 .0125-.0140 0.34 0.006 0.15 2.17

22s .0280-.0285 0.72 .0055-.0065 0.15 0.011 0.28 0.45

22 .0280-.0285 0.72 .0155-.0170 0.41 0.006 0.15 3.35

21 .0320-.0325 0.82 .0195-.0210 0.51 0.006 0.15 5.19

20 .0355-.0360 0.91 .0230-.0245 0.60 0.006 0.15 6.71

18 .0495-.0505 1.27 .0315-.0345 0.84 0.0085 0.22 14.08

17 .0575-.0580 1.47 .0405-.0435 1.07 0.008 0.20 22.84

16 .0645-.0655 1.65 .0455-.0485 1.19 0.009 0.23 28.25

14 .0820-.0840 2.11 .0610-.0600 1.60 0.010 0.25 51.07

13 .0940-.0960 2.41 .0690-.0730 1.80 0.012 0.31 64.63

12 .1080-.1100 2.77 .0830-.0870 2.16 0.012 0.31 93.07

11 .1190-.1210 3.05 .0920-.0960 2.39 0.013 0.33 113.00

10 .1330-.1350 3.40 .1040-.1080 2.69 0.014 0.36 143.28

NominalO.D. NominalI.D. WallThickness Volume

GaugeIndexTable

*mm are nominal

Custom Needle Gauge and Length

Whenselectinganeedlegaugeitisimportanttokeepinmindthevolumeofthesyringeandthedeadvolumeoftheneedle.Forexample,itwillbeverydifficulttoprimea10µLsyringeifthedeadvolumeintheneedleisgreaterthan10µL.RefertothegaugeindextochooseaneedlegaugewithanappropriateµL/inchbeforeselectinganeedle.Selecttheminimumlengththatallowsyoutocarryoutyourapplicationcomfortably.


Note: The ‘s’ on a 22s needle represents a smaller I.D. (inner diameter) for the needle and a thicker needle wallfor better durability. For example, a 26 gauge needle has an O.D. (outer diameter) of 0.46 mm and an I.D. of 0.26 mm while the 26s gauge needle has an O.D. of 0.47 mm and an I.D. of 0.13 mm. The 26s has half the I.D. of the 26 gauge needle. Also, the difference in the wall thickness nearly doubles with 26s gauge having a thickness of 0.18 mm while the 26 gauge is only 0.10 mm.


Accessories, Replacement Parts, and Services

Hamiltonoffersavarietyofaccessoriestoimprovedurabilityandreproducibility,includingtheonesdescribedbelow,aswellasreplacementpartsforoursyringes.Detailscanbefoundatwww.hamiltoncompany.com

Cleaning Concentrate

Theconcentrateisabiodegradablecleaningagentforremovalofstubbornresidues.HamiltonPartNo.18311(500mL).

Needle Cleaning Kit

Containsaselectionofvariousdiametertungstenwiresaswellasabiodegradablecleaningconcentrateforcleaningpluggedneedles.HamiltonPartNo.76620.Additionalcleaningwiresandconcentratecanbepurchasedseparately.

Syringe Cleaner

Theunitisdesignedtoclean7000SeriesMICROLITER™syringeswithonlyheat(370°C)oraddavacuumsource(0.1mmmercury)toremovesuspectedresiduals.HamiltonPartNo.76610(120VAC)andPartNo.76615(220VAC).

Syringe Guide

Theguideiseasilyinstalledonasyringetopreventtheplungerfrombendingorbeingpulledout.Twomodelsareofferedfordifferentsyringevolumesandseries.

Reproducibility (Chaney) Adapter

TheChaneyAdapteriseasilyinstalledonasyringeforconsistent,reproducibleinjections.Also,theadapterpreventsplungerbendingwhileanadjustablestopprovidesincreasedprecisionandaccuracy.Fourmodelsareavailabletoaccommodatearangeofsyringevolumesandseries.

PB600 Repeating Dispenser

ThePB600(HamiltonPartNo.83700)canbeusedwithliquidsorgasestoconsistentlydispense1/50thofthesyringevolume.ThedispenserfitsMICROLITERandGASTIGHTsyringeswithvolumesupto2.5mL.

Digital Syringe™

ThebaseunitcanbeusedwithHamiltonsyringesinthe700,1700,and7000serieswithnominalvolumesbetween0.5µLand500µL.Aneasy-to-readLCDscreendisplaysthevolumecontainedinthesyringetowithin±0.5%ofthesyringe’snominalvolume.TheDigitalSyringeisorderedbyadding‘DS’asaprefixtotherequiredsyringepartnumber.

DigitalSyringesareautomaticallyN.I.S.T.traceablycalibratedtothebaseunitpriortoshipment.RecalibrationserviceisavailablefortheDigitalSyringe.ContactHamiltonCustomerServiceDepartmenttoobtainanRGANumber(ReturnedGoodsAuthorizationNumber).IncludethesyringepartnumberusedwiththebaseunitontheRGAandreturnthedigitalunitwithoutthesyringe.Thecustomerwillbechargedthecalibrationfeeplusthecostofanewsyringe.

N.I.S.T. Traceable Certification

Thiscalibrationserviceisavailableformostofourprecisionsyringes.ACertificateofCalibrationisshippedwiththeproductandtheprocedureisperformedwithanunbrokenchainofcalibrationswithN.I.S.T.traceableweights.Calibratedsyringesmustbespecifiedatthetimeoforderingbyaddingtheprefix‘CAL’tothebeginningofthesyringe’spartnumber.Forexample,toordera701N,10µLsyringe(HamiltonPartNo.80300)asacalibratedsyringe,requestPartNo.CAL80300.

Frequently Asked Questions

ManyofyourquestionscanbeansweredbyvisitingtheFAQpageofourwebsiteatwww.hamiltoncompany.com

Online

Ourtechnicalstaffwillpromptlyanswerquestionssentbyemailtosales@hamiltoncompany.com

Telephone

Forallothertechnicalissues,call1-888-525-2123forHamiltonTechnicalServiceassistance.ForassistanceoutsidetheU.S.,contactyourlocaldealer.


Additional Technical Information at www.hamiltoncompany.com

Hamilton Precision Syringes Care and Use

Withpropercareandhandling,Hamiltonsyringeswillprovideunsurpassedperformanceyearafteryear.SeeourcompleteGuide to Maintaining and Using Hamilton GASTIGHT®, MICROLITER™, and Specialty Syringes.

Determining the Performance of Hamilton Syringes

Followtheprotocolonthisdocumenttoconfirmtheaccuracyofasyringe.TheHamiltonCompanyQualitySystemisISO9001-2000certified.

Inner and Outer Dimensions

Forapplicationsandprojectswherethephysicaldimensionsofasyringeareimportant,specificationsareprovidedforthemostpopularsyringesinourproductline.

Product Instruction Sheets

Electronicversionsofthedocumentationshippedwithnewproductscontaininginformationonassembly,use,replacementparts,etc.Refertothesesheets,ifyouhavemisplacedanoriginalinstructionsheetorwouldliketoseemoreinformationonaspecificproductpriortopurchase.

Syringe Graduations

Occasionally,usershaveaskedforinformationrelatingtothescaledivisionsonasyringetothedeliveryvolume.Aseriesoftablesdetailthisinformationforallofoursyringes.

Thefollowinginformationisavailableonourwebsiteandaspdfs.

Technical Support

http://www.hamiltoncompany.comSales and Support USA 1-888-525-2123

Hamilton Company4970 Energy WayReno, Nevada 89502Toll-Free: 800-648-5950Telephone: 775-858-3000Fax: 775-856-7259Email: [email protected]

Hamilton Bonaduz AGVia Crusch 8CH-7402 Bonaduz/SwitzerlandTelephone: 00800-660-660-60Fax: +41-(0)81-660-60-70Email: [email protected]

Quality Hamilton Products–MICROLITER™ Syringes GASTIGHT® SyringesChromatography SyringesSyringes for Life ScienceInstrument SyringesSoftGrip™ PipettesMiniature ValvesModular Valve PositionerLaboratory Fittings, Adapters, and Tubing Precision Syringe PumpsMICROLAB® Diluters and Dispensers Electrochemical SensorsDURACAL™ Buffer SolutionsHPLC Columns and Resins

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The following are trademarks of Hamilton CompanyAdvanTip™

Pipette Safe™

SoftGrip™

SofTouch™

SofTop™

SoftAide™

The following company trademarks have been used in this catalogPCR protected by patents issued to F. Hoffman-La Roche Ltd.

Lit. No. L70057 © Hamilton Company 5/06 10k Printed in U.S.A.

Documents

SYRINGE SELECTION - Greyhound Chromatography · 1. Syringe Types Key to selecting the right syringe for your application is to identify your sample type and determine the smallest