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GRiSP Integrating physiology, Crop Modeling and Genetics to Tackle Thermal Stresses in Rice: The RIDEV Approach Michael Dingkuhn (IRRI/CIRAD) Cecile Julia (Ph.D. 2012, Montpellier) Richard Pasco (IRRI) Jean-Christophe Soulie (CIRAD) funded by GEZ, AfricaRice, CCAFS and CIRAD

The RIDEV Approach · Panicle T for heat stress at anthesis Time of day of anthesis (TOA) Towards a new RIDEV Funding: GEZ (Risocas project) and ARC (modeling ... Microclimate recording

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Page 1: The RIDEV Approach · Panicle T for heat stress at anthesis Time of day of anthesis (TOA) Towards a new RIDEV Funding: GEZ (Risocas project) and ARC (modeling ... Microclimate recording

GRiSPIntegrating physiology, Crop

Modeling and Geneticsto Tackle Thermal

Stresses inRice:

The RIDEV Approach

Michael Dingkuhn (IRRI/CIRAD)Cecile Julia (Ph.D. 2012, Montpellier)

Richard Pasco (IRRI)Jean-Christophe Soulie (CIRAD)

funded by GEZ, AfricaRice, CCAFS and CIRAD

Page 2: The RIDEV Approach · Panicle T for heat stress at anthesis Time of day of anthesis (TOA) Towards a new RIDEV Funding: GEZ (Risocas project) and ARC (modeling ... Microclimate recording

GRiSP Topics

� Thermal factors affecting rice production

� History: Sahel irrigated rice and the 1st RIDEV tool

� New aspects: Transpiration cooling and TOA

� A multi-environment study on rice microclimate

� Modeling micro-climate based phenology and sterility with RIDEV V.2

� Applications of RIDEV V.2

� Mapping of thermal risks

� Heuristic phenotyping of phenology and thermal sterility

� Outlook

Page 3: The RIDEV Approach · Panicle T for heat stress at anthesis Time of day of anthesis (TOA) Towards a new RIDEV Funding: GEZ (Risocas project) and ARC (modeling ... Microclimate recording

GRiSP Rationale

� Thermal adaptation is fundamental for agro-ecological fit and fitness

� CC is changing thermal environments

� Rice as species has diverse thermal adaptation, but broadly adapted genotypes are rare

� Accuracy of crop models is still poor re. thermal effects

� We need…

� Better predictive tools to map CC impact

� Better understanding of adaptive traits

Page 4: The RIDEV Approach · Panicle T for heat stress at anthesis Time of day of anthesis (TOA) Towards a new RIDEV Funding: GEZ (Risocas project) and ARC (modeling ... Microclimate recording

GRiSP

Micro-climateWeather

Some thermal factors affecting rice production

Pheno-logy

Yield potential

canopy architecture

Ta Tmer

YieldT

pan

TOA

Resources

=> A, R

PP

RH

Rs

Genetic constitution:- Pollen production- Physiol. tolerance

Studied hereNot studied here

Spikeletsterility

Page 5: The RIDEV Approach · Panicle T for heat stress at anthesis Time of day of anthesis (TOA) Towards a new RIDEV Funding: GEZ (Risocas project) and ARC (modeling ... Microclimate recording

GRiSP

India NARES

Research Landscape

IRRI

AfricaRice

CIRAD

NIAES

Hohen-heim

ORYTAGECrop model assisted

phenotyping

RISOCASCrop-climate intercations

GRiSPPhenotypingNetwork

IM2PACT projectRadiation balance modeling, CO2 interaction

1990s RIDEV project : Physiology & decision aid tools

Jagadish’s networks

Page 6: The RIDEV Approach · Panicle T for heat stress at anthesis Time of day of anthesis (TOA) Towards a new RIDEV Funding: GEZ (Risocas project) and ARC (modeling ... Microclimate recording

GRiSP

History

Sahel irrigated rice and the1st RIDEV tool

Page 7: The RIDEV Approach · Panicle T for heat stress at anthesis Time of day of anthesis (TOA) Towards a new RIDEV Funding: GEZ (Risocas project) and ARC (modeling ... Microclimate recording

GRiSPHistory 1 (WARDA 1990s): Thermal constraints to irrigated rice in Senegal

Sowing date vs. crop duration and sterility

⇒ Thermal and photoperiod effects on phenology⇒ Chilling causes spikelet sterility

Days to flowering

% sterility % sterility

Tw(min) at bootingSowing dateSowing date

Page 8: The RIDEV Approach · Panicle T for heat stress at anthesis Time of day of anthesis (TOA) Towards a new RIDEV Funding: GEZ (Risocas project) and ARC (modeling ... Microclimate recording

GRiSP

8

BVP

PSP

REP

Filling

germ

PI

F

mat

Ta pollination

TwMicro-spore-genesis

PPphenology

T-sensitive phases

Taphenology

Twphenology

=> Water temperature governs phenologyand chilling-induced sterility in flooded rice

Tw(max)

History 2: basis of 1995 RIDEV

Page 9: The RIDEV Approach · Panicle T for heat stress at anthesis Time of day of anthesis (TOA) Towards a new RIDEV Funding: GEZ (Risocas project) and ARC (modeling ... Microclimate recording

GRiSP

Sterility probability 1950-1982

History 3: Risk analysis with RIDEV / crop calendar s

Saint-Louis

Rosso

Matam

Tillaberi

Sterility(heat)

Sterility(cold)

Sowing date

% Sterility

1 year

Page 10: The RIDEV Approach · Panicle T for heat stress at anthesis Time of day of anthesis (TOA) Towards a new RIDEV Funding: GEZ (Risocas project) and ARC (modeling ... Microclimate recording

GRiSP

Zoning of latest possible sowing date for Sahel wet se ason irrigated rice crop

History 4: Mapping sterility risks with RIDEV

Page 11: The RIDEV Approach · Panicle T for heat stress at anthesis Time of day of anthesis (TOA) Towards a new RIDEV Funding: GEZ (Risocas project) and ARC (modeling ... Microclimate recording

GRiSP New study on rice phenology and sterility responses to T

(Thesis of Cecile Julia)

� Emphasis on microclimate� Meristem T for phenology

� Floodwater T for chilling stress at microspore stage

� Panicle T for heat stress at anthesis

� Time of day of anthesis (TOA)

� Towards a new RIDEV

� Funding: GEZ (Risocas project) and ARC (modeling research at IRRI)

Page 12: The RIDEV Approach · Panicle T for heat stress at anthesis Time of day of anthesis (TOA) Towards a new RIDEV Funding: GEZ (Risocas project) and ARC (modeling ... Microclimate recording

GRiSP

BVP PSP Reproductive Maturation

PI

Panicle T at anthesis=> Heat sterility

Water tmin=> Cold sterility

Canopy T=> Poor panicle exertion=> Sterility

Day length

TOA

Development ratefn(thermal time)

Apex is below water line

Apex

emerges Apex at top of canopy

Page 13: The RIDEV Approach · Panicle T for heat stress at anthesis Time of day of anthesis (TOA) Towards a new RIDEV Funding: GEZ (Risocas project) and ARC (modeling ... Microclimate recording

GRiSP

Date01/08 11/08 21/08 31/08 10/09 20/09 30/09

Tem

pera

ture

(°C

)

8

12

16

20

24

28

32

36

40

44

Date

01/03 11/03 21/03 31/03 10/04 20/04 30/04

Tem

pera

ture

(°C

)

8

12

16

20

24

28

32

36

40

44

Tair Max Tair Min Twater max Twater min

Date

15/01 25/01 04/02 14/02 24/02 06/03 16/03 26/03

Tem

pera

ture

(°C

)

8

12

16

20

24

28

32

36

40

44

Date10/05 20/05 30/05 09/06 19/06

Tem

pera

ture

(°C

)

8

12

16

20

24

28

32

36

40

44

France - Temperate Summer 2009

VP

D (

KP

a)

012345

Senegal - Cold and dry season 2010

VP

D (

KP

a)

012345

Senegal - Hot and dry season 2010

VP

D (

KP

a)

012345

Philippines - Hot and dry season 2009

VP

D (

KP

a)

012345

Thesis study:(Cecile Julia)

4 genotypesIR64IR72Sahel108Chomrong(N22 failed)

4 environments

DS PhilippinesHDS SenegalCDS SenegalTemp. summer France

Traits observedPhenologyTOAPanicle Tr cooling

Page 14: The RIDEV Approach · Panicle T for heat stress at anthesis Time of day of anthesis (TOA) Towards a new RIDEV Funding: GEZ (Risocas project) and ARC (modeling ... Microclimate recording

GRiSP

Mean Ta(min) during last 7d before anthesis ( oC)

Time of day of anthesisshows adaptive plasticity

Warm nights advance TOA => Escape midday heat

Humid days advance TOA => escape heat caused by absence of transpiration cooling

Results

Page 15: The RIDEV Approach · Panicle T for heat stress at anthesis Time of day of anthesis (TOA) Towards a new RIDEV Funding: GEZ (Risocas project) and ARC (modeling ... Microclimate recording

GRiSP

20

22

24

26

28

30

32

34

36

38

40

0 20 40 60 80 100 120 140 160 180 200

Hou

rly a

vera

ge T

C)

Position from the ground (cm)

(a)

inside canopy

above

canopypanicle layer

28/05/2010Water

7:00

8:00

9:00

10:00

11:00

12:00

13:00

Time

25

26

27

28

29

30

31

32

33

34

0 20 40 60 80 100 120 140 160 180 200

Hou

rly a

vera

ge T

C)

Position from the ground (cm)

(b)

inside

canopy

above

canopy

panicle layer

16/04/2009Water

7:00

8:00

9:00

10:00

11:00

12:00

13:00

Time

Microclimate in IR64 canopy: Examples of sunny days

Senegal, hot-dry season Philippines, dry-season

Page 16: The RIDEV Approach · Panicle T for heat stress at anthesis Time of day of anthesis (TOA) Towards a new RIDEV Funding: GEZ (Risocas project) and ARC (modeling ... Microclimate recording

GRiSPPan1

Pan2

Flagleaf1

Flagleaf2

Pan3

Pan4

Flagleaf3

Flagleaf4

Leaf5

Ca. 4900 IR observations on in-situ panicle TMicroclimate recordingAgronomic observations incl. %sterility

Panicle temperature

Page 17: The RIDEV Approach · Panicle T for heat stress at anthesis Time of day of anthesis (TOA) Towards a new RIDEV Funding: GEZ (Risocas project) and ARC (modeling ... Microclimate recording

GRiSP

y = 1.66x - 1.31R² = 0.70

-4

-2

0

2

4

6

8

10

12

14

0 1 2 3 4 5 6 7

TD

=Ta-

Tp

( °C

)

VPD (kPa)

b

y = 1.45x - 0.99R² = 0.79

-4

-2

0

2

4

6

8

10

12

14

0 1 2 3 4 5 6 7

TD

=Ta-

Tp

( °C

)

VPD (kPa)

cy = 2.07x - 0.59R² = 0.15

-4

-2

0

2

4

6

8

0.5 1.0 1.5 2.0

TD

=Ta-

Tp

( °C

)

VPD (kPa)

a

Panicle-air temperature difference

y = 2.07x -0.59

R² = 0.15

-4

-2

0

2

4

6

8

0.5 1.0 1.5 2.0

TD

=Ta-

Tp

( °C

)

VPD (kPa)

a

Philippines Senegal hot-dry season Senegal cool-dry s eason

Page 18: The RIDEV Approach · Panicle T for heat stress at anthesis Time of day of anthesis (TOA) Towards a new RIDEV Funding: GEZ (Risocas project) and ARC (modeling ... Microclimate recording

GRiSP

TD (predicted) [°C]

-4 -2 0 2 4 6 8 10 12 14

TD

(ob

serv

ed)

[°C

]

-4

-2

0

2

4

6

8

10

12

14

Senegal cool-dryseasonSenegal hot-dryseasonFrance summer

1:1

Multiple regression model predicting TD from…VPD, Rs, Ta, Sun angle, and Panicle height from groundTD1 = -1.578 + 1.093*VPD+0.189*Tair - 3.367E-02*TopP-3.99E-03*Rs + 3.31E-02*Hdeg

Panicle-air temperature difference (=>RIDEV): Regression model adopted for simulation

Page 19: The RIDEV Approach · Panicle T for heat stress at anthesis Time of day of anthesis (TOA) Towards a new RIDEV Funding: GEZ (Risocas project) and ARC (modeling ... Microclimate recording

GRiSPy = 0.56x - 0.70

R² = 0.72

-15

-10

-5

0

5-15 -5 5

Tp-

Ta o

bsev

ed (°

C)

Tp-Ta calculated ( °C)

CHOMRONG

IM2PACTcalc vs…

y = 0.86x - 0.56R² = 0.76

-15

-10

-5

0

5-15 -5 5

Tp-

Ta o

bsev

ed (°

C)

Tp-Ta calculated ( °C)

CHOMRONG

RIDEVcalc vs…

y = 0.74x - 1.14R² = 0.83

-15

-10

-5

0

5-15 -5 5

Tp-

Ta o

bsev

ed (°

C)IR64

IM2PACTcalc vs…

y = 1.05x - 0.06R² = 0.87

-15

-10

-5

0

5-15 -5 5

Tp-

Ta o

bsev

ed (°

C)IR64

RIDEVcalc vs…

y = 1.13x + 0.20R² = 0.81

-15

-10

-5

0

5

-15 -5 5 Tp-

Ta o

bsev

ed (°

C)

Tp-Ta calculated ( °C)

Sahel 108

RIDEVcalc vs…

y = 0.77x - 1.07R² = 0.74

-15

-10

-5

0

5

-15 -5 5 Tp-

Ta o

bsev

ed (°

C)

Tp-Ta calculated ( °C)

Sahel 108

IM2PACTcalc vs…

RIDEV vs.

IM2PACT(Tsukuba, field-

calibrated in Japan)

Page 20: The RIDEV Approach · Panicle T for heat stress at anthesis Time of day of anthesis (TOA) Towards a new RIDEV Funding: GEZ (Risocas project) and ARC (modeling ... Microclimate recording

GRiSP

22

24

26

28

30

32

PHIL_DS SEN_HS SEN_CS FR_HS

Tem

pera

ture

C)

Site

(b)

Air and Panicle Temperature at TOA (calculated)

22

24

26

28

30

32

Chomrong IR64 IR72 S108

Tem

pera

ture

C)

Variety

(a)

Site means

Genotype means

Page 21: The RIDEV Approach · Panicle T for heat stress at anthesis Time of day of anthesis (TOA) Towards a new RIDEV Funding: GEZ (Risocas project) and ARC (modeling ... Microclimate recording

GRiSP

Disaggregation of observed sterility into its components

- Incomplete panicle exertion- Chilling at microspore stage- Heat at anthesis (TOA)

Page 22: The RIDEV Approach · Panicle T for heat stress at anthesis Time of day of anthesis (TOA) Towards a new RIDEV Funding: GEZ (Risocas project) and ARC (modeling ... Microclimate recording

GRiSP

40

60

80

100

120

140

160

PHIL_DS SEN_HS SEN_CS FR_HS

Pan

icle

exs

ertio

n (%

)

Site

Chomrong IR64 S108

IR72 Last grain Neck node

(b)

Panicle exertion at flowering

0

10

20

30

40

50

60

70

80

90

100

PHIL_DS SEN_HS SEN_CS FR_HS

Ste

rility

(%

)

Site

(c)

Chomrong IR64 S108 IR72

Spikelet sterility Incomplete panicle exertion- occurred in cold-night environments- explained some of observed sterility

Observed spikelet sterility

Page 23: The RIDEV Approach · Panicle T for heat stress at anthesis Time of day of anthesis (TOA) Towards a new RIDEV Funding: GEZ (Risocas project) and ARC (modeling ... Microclimate recording

GRiSP

0

10

20

30

40

50

60

70

80

90

100

12 14 16 18 20 22 24 26 28

Ste

rility

(%

)

Tw(min) at microspore stage ( °C)

Phil-ds Sen-hs

Sen-cs Fr-hs

Chomrong

Chilling effect at microspore stage on sterility

Page 24: The RIDEV Approach · Panicle T for heat stress at anthesis Time of day of anthesis (TOA) Towards a new RIDEV Funding: GEZ (Risocas project) and ARC (modeling ... Microclimate recording

GRiSP Heat effect at anthesis on sterility

Page 25: The RIDEV Approach · Panicle T for heat stress at anthesis Time of day of anthesis (TOA) Towards a new RIDEV Funding: GEZ (Risocas project) and ARC (modeling ... Microclimate recording

GRiSPConclusions of experimental study

(Thesis C. Julia)

Reproductive-stage adaptations to thermal stresses:

� Tolerance� Pollen viability under heat/cold (anti-oxidants? OA ?

Membrane & protein properties?)

� Avoidance� Transpiration cooling of panicle� Good panicle exertion (long peduncle)

� Escape� Time of day of anthesis (TOA)� Adaptive plasticity of TOA

� Temporal spread of anthesis (e.g., 2 wk)

Heat stress more likely in warm -humid than hot-dry climates!

Page 26: The RIDEV Approach · Panicle T for heat stress at anthesis Time of day of anthesis (TOA) Towards a new RIDEV Funding: GEZ (Risocas project) and ARC (modeling ... Microclimate recording

GRiSP

Modeling with RIDEV

Page 27: The RIDEV Approach · Panicle T for heat stress at anthesis Time of day of anthesis (TOA) Towards a new RIDEV Funding: GEZ (Risocas project) and ARC (modeling ... Microclimate recording

GRiSP

BVP PSP Reproductive Maturation

PI

Panicle T at anthesis=> Heat sterility

Water tmin=> Cold sterility

Canopy T=> Poor panicle exertion=> Sterility

Day length

TOA

Development ratefn(thermal time)

Apex is below water line

Apex

emerges Apex at top of canopy

Processes considered

in RIDEV

Page 28: The RIDEV Approach · Panicle T for heat stress at anthesis Time of day of anthesis (TOA) Towards a new RIDEV Funding: GEZ (Risocas project) and ARC (modeling ... Microclimate recording

GRiSP RIDEV, new tool for crop -model assisted phenomics

� Simulator of…� Phenology incl. microclimate & PP effects� Sensitive phases to T of reproductive processes� G and E effects on TOA� Sterility caused by…

� Chilling effects on microsporogenesis (water Tmin)� Chilling effects on panicle exertion (air Tmin)� Heat effects on pollination (Tpanicle at anthesis)

� Prediction (forward mode)� CC impact mapping, plant type optimization� Agronomy (optimize genotype and crop calendar; risk studies)

� Heuristic parameterization of genotypes (reverse mo de)� Phenomics (extraction of genotypic parameter values from

experimental data)

Page 29: The RIDEV Approach · Panicle T for heat stress at anthesis Time of day of anthesis (TOA) Towards a new RIDEV Funding: GEZ (Risocas project) and ARC (modeling ... Microclimate recording

GRiSP RIDEV Interface for point simulationsHTP Optimization of genotypic parameters by R-Genoud (genetic algo) against observation file (heuristics):

- Tbase, Topt- Duration BVP & Matu- PP-sensitivity (4 models)- crit. T Cold1, Cold2, Heat

Programming languages: C++, R

Page 30: The RIDEV Approach · Panicle T for heat stress at anthesis Time of day of anthesis (TOA) Towards a new RIDEV Funding: GEZ (Risocas project) and ARC (modeling ... Microclimate recording

GRiSP

Spi

kele

t ste

rility

(to

tal,

as fr

actio

n)

0.0

0.2

0.4

0.6

0.8

1.0Total sterility (sim.)Total sterility (obs., ORYTAGE)Total sterility (obs., thesis Stuerz)

Error bars = SE (ORYTAGE data)

cv. Sahel 108, Ndiaye, Senegal

Cro

p du

ratio

n (d

)

40

60

80

100

120

140

160Flowering (sim.)Maturity (sim.)Flowering (obs., ORYTAGE)Flowering (obs., thesis Stuerz)Maturity(obs., ORYTAGE)Maturity (obs., thesis Stuerz)

Sowing date

Spi

kele

t ste

rility

(co

mpo

nent

s, a

s fr

actio

n)

0.0

0.2

0.4

0.6

0.8

1.0

SterCold1 (sim.)SterCold2 (sim.)SterHeat (sim.)

2008 2009 2010

2008 2009 2010

2008 2009 2010

Model parameters:Tbase = 10.6 oCTopt = 31.7 oCPPexp = 0.281PPsens = 1.032SumBvp = 748 oC.dSumRepr = 400 oC.d (preset)SumMatu = 409 oC.dCritSterCold1 = 10.6 oCCritSterCold2 = 18.7 oCCritSterHeat = 29,7 oCSterBase = 0.08 (=8%)

Example offitting RIDEV

-SAHEL 108- Many sowing dates- Different data sources

BUT: still problems with fitting some trad. cvs.

Observed duration to flowering (d)

60 80 100 120 140

Sim

ulat

ed d

urat

ion

to fl

ower

ing

(d)

60

80

100

120

Senegal

Y = 7.5 + 0.91X, R2 = 0.92Madagascar

Page 31: The RIDEV Approach · Panicle T for heat stress at anthesis Time of day of anthesis (TOA) Towards a new RIDEV Funding: GEZ (Risocas project) and ARC (modeling ... Microclimate recording

GRiSP Issues

� Methodological (RIDEV)

� Phenology of some traditional cvs. is difficult to simulate (complex T x PP interaction?)

� More validation needed before extrapolation

� New scientific questions

� Trade-offs between water-saving and transpiration cooling (extend RIDEV to water limited situations)

� Transpiration cooling under high CO2?

� Genetics of heat avoidance (transpiration cooling) and escape (adaptive plasticity of TOA)

Page 32: The RIDEV Approach · Panicle T for heat stress at anthesis Time of day of anthesis (TOA) Towards a new RIDEV Funding: GEZ (Risocas project) and ARC (modeling ... Microclimate recording

GRiSP Outlook (1)Use of RIDEV for Phenomics /GWAS

� Indica GWAS panel (200 acc., ORYTAGE project)

� Field-phenotyped for phenology and sterility in 12 en vironments:� 6 sowing dates in Senegal� 3 altitudes x 2 years in Madagascar

� Extraction of genotypic response parameters across environments:� Cardinal temperatures Tb and To� Thermal duration of phenological phases� PP-sensitivity� Chilling sensitivity of microsporogenesis� Chilling sensitivity of panicle exertion� Heat sensitivity of anthesis

� Association study using 700K Oryza SNP chip

Page 33: The RIDEV Approach · Panicle T for heat stress at anthesis Time of day of anthesis (TOA) Towards a new RIDEV Funding: GEZ (Risocas project) and ARC (modeling ... Microclimate recording

GRiSPAPO 91-385 DA5 JAMAJIGI PA TOU HUNG TAICHUNG NATIVE 1 WAB 706-3-4-K4-KB-1

ASD 1 93-11 DA9 JC120 PAPPAKU TAMCAU 9 AWAS105-B-IDSA-B-WAS2-1-FKR-1

AZUCENA ADNY 11 DANAU LAUT TAWAR JC91 PATIK TELIMANI WAS169-B-B-4-2-1BASMATI 370 AI CHIAO HONG DE ABRIL JENGAR PEH KUH TELOVOLANA 177 WAS170-B-B-1-1BULU PANDAK ANGIFOTSY 685 DHOLA AMAN KALILA MENA PEH KUH TSAO TU TETEP WAS173-B-B-6-2-2DOM SOFID APURA DOURADO AGULHA KALINGA III PELITA JANGGUT THAPACHINIYA WAS174-B-3-5DULAR ARANG DR92 KARKATI 87 PETA THIERNO BANDE WAS181-B-6-3FANDRAPOTSY 104 B2997C-TB-4-2-2-1 FEDEARROZ 2000 KASALATH PIN TAWNG TOKAMBANY 663 WAS182-B-1-1FR13A B6144-MR-6-0-0 FEDEARROZ 50 KATI POKKALI TOKAMBANY 669 WAS183-B-6-2-3WAS 33 B-B BADKALAMKATI GAJPATI KAW LUYOENG POKKALI TSAKA WAS194-B-3-2-5GIZA 171 BAGUAMON 14 GAMBIAKA KOKOUM KHAO HAWM POPOT TSIPALA 1231 WAS197-B-6-3-11IAC 165 BALA GIE 57 KIANG CHOU CHIU PTB 25 TSIPALA B160 WAS198-B-3-1-3IR64 BENGALY 389 GOCHI BORO KITRANA 1890 PURBIA TSIPALA FOTSY 1883 WAS199-B-1-2-1KAUKKYI ANI BETSILAIZINA GOPAL KITRANA 508 RATHAL 2 TSIPALA MENA 626 WAS200-B-B-1-1-1KHAO DAM BG90-2 GUAN YIN TSAN KOGONI 91-1 RATHUWEE UPL RI-5 WAS202-B-B-1-1-2KHAO DAWK MALI 105 BH2 GWA NGASEIN LAITRA RAY JAZAYKAYZ UPL RI-7 WAS203-B-B-2-4-1M 202 BODOMANO H15-23-DA LAL AMAN RAY NABJA VANDANA WAS206-B-B-2-2-1

MOROBEREKAN BOTOHAVANA 139 HASAN SERAI LATSIBOZAKA-112-1 ROJOFOTSY 693VARY LAVA DE MAROVATO WAS207-B-B-3-1-1

N 22 BOTOHAVANA B13 IM 16 LOHAMBITRO 224 ROJOKELY VARY MADINIKA 3494 WAS208-B-B-5-1-1-3NIPPONBARE BOTRA MAITSO IR1561-228-3-3 MACAN BINUNDOK ROJOMENA 1034 VARY VATO 154 WAS20-B-B-1-2-2TEQUING BOTRIKELY IR19746-28-2-2 MADINIKA 1329 ROJOMENA B48 VARY VATO 462 WAS21-B-B-20-4-3-3

BOTRY 731 IR20 MAKALIOKA 34 RTS 12 VATO MATSOAMALONA WAS30-11-4-6-2-2-1BOUAKE 189 IR2006-P12-12-2-2 MALADY RTS 14 ZALCHA WAS33-B-B-15-1-4-5

Chromrong BR24 IR22 MAMORIAKA 114 RTS 4 ARC15872 WAS49-B-B-9-1-4-2

SAHEL 201 C21 IR2307-247-2-2-3MANGAVAVA FOTSILANSTSIKA 1177 RTS 5 ELONI WAS50-B-B-24-4-2-1

C4 63G IR2344-P1PB-9-3-2B MENAHODITRA 1234 S 624 WASSA WAS55-B-B-2-1-2-5CARREON IR28 MTU 9 SAHEL 108 WAY RAREM WAS57-B-B-3-1-4-6CERE AIR IR36 NAM ROO SAHEL 159 WAS62-B-B-17-1-1-3CHAU IR5 NAM SA GUI 19 SAHELIKA WAS63-22-5-9-10-1CHERIVIRUPPU IR50 NGAJA SALUMPIKITCHIEM CHANH IR52 NIONOKA SAMBALA MALOCHITRAJ IR53236-275-1 NONA BOKRA SAO

CICA 8 IR55411-50 O LUEN CHEUNG SATHI34-36

CT6510-24-1-2 IR55419-04 ORYZICA LLANOS 5 SEBERANG MR77IR57920-AC-25-2-B ORYZICA SABANA 10 SEBOTA 65IR57924-24 SHAI KUHIR60 SHORT GRAINIR62266-42-6-2 SINNA SITHIRA KALIIR72 SINTANE DIOFORIR74371-54-1-1 SOMCAU 70 AIR8 SOMIZY

SONASWARNA Indica panel (200)

ARC cvs.

IRRI cvs

Oryza SNP panel (all genetic groups)

A rich phenomics resource available for phenoloy & sterility:

- Madagascar: 3 altitudes x 2 yrs -Senegal: 6 sowing dates

⇒ RIDEV para optimization⇒ GWAS

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GRiSP Outlook (2)Use of RIDEV for CC impact mapping and

agronomic decision support

� Joint global mapping of thermal risks with IRRI’s GIS group (A Nelson)

� CCAFS collaboration: Future climate scenarios

� RIDEV incorporation into Rice Manager for Africa

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GRiSP

Thank youMerciSalamat po