Page 1

The Right Solution:

The Agilent 1200 Infinity

Series for LC Analysis

Laura Phillips

Application Scientist

Agilent Technologies

June 27th, 2013

Page 2

The Agilent 1200 Infinity Series Where Do We Come From?

June 27, 2013

1100 Bin/Quat

HPLC

Bin (HPM)

Quat (LPM)

400 bar

5 mL/min

1290 Infinity (2009)

UHPLC

Bin (HPM)

Quat (LPM - 2012)

1200 bar

5 mL/min

1260 Infinity Bin/Quat

UHPLC

Bin (HPM) – 5mL/min

Quat (LPM) – 10mL/min

600 bar

Price ~ 1200 Bin/Quat

1220 Infinity

UHPLC

Bin (LPM)

600 bar, 5 mL/min

Price < 1200 Quat

1200 Infinity Series

1200 Bin/Quat

HPLC

Bin (HPM)

Quat (LPM)

400 bar

5 mL/min

1200 RRLC

UHPLC

Bin (HPM)

600 bar

5 mL/min

Price: 1200 Bin+20%

1200 Series (2006 – 2010)

1100 Series (1995 – 2006)

Page 3

The Agilent 1200 Infinity Series Raising the Standard in HPLC – UHPLC for EVERY Lab

June 27, 2013

1200 Infinity Series

1220 Infinity

Isocratic, manual

Gradient, manual

Gradient, automated

Gradient-DAD

1260 Infinity

Low-flow

Cap.LC

Nano-LC

Chip-LC

Analytical flow

Binary

Quaternary

Isocratic

Binary-SFC

Quaternary Bio-Inert

Prep-flow

1290 Infinity

Binary

Quaternary

new

new

Page 4

The Agilent 1200 Infinity Series Raising the Standard in HPLC – UHPLC for EVERY Lab

June 27, 2013

Method Developement

Solution

Multi-method Solution

High-Dynamic Range DAD

2D-LC Solution

Online-SPE Valve solutions Bioinert

solutions SFC solutions

GPC solutions

Page 5

1220 Infinity LC A solution to all your needs

• Dual gradient pump with integrated

degasser, 600 bar, up to 10 mL/ min, for

flexible and reliable analysis

• Vial sampler with injector programming for

higher automation

• 80°C oven for one 25 cm column for better

results

• Variable wavelength or diode array

detector with 80 Hz sampling rate for high

sensitivity

June 27, 2013

• Easy: Small footprint, low height. One

cable.

• Affordable with ChemStation/ EZChrom

VL software

• Expandable: Any other module can be

added

Page 6

600 bar for HPLC and UHPLC

June 27, 2013

Same as with the 1260 Infinity LCs, existing conventional HPLC methods

from 1100/1200 Series LC can be run on the 1220 Infinity LC and giving the

same results as well as UHPLC methods on 3.0mm and 4.6mmID columns

can be run. Both with excellent performance!

Saving half of the time by transferring a HPLC

method to UHPLC, without loss of resolution. Excellent retention and area RSDs even under

UHPLC conditions.

Page 7

Expandability

June 27, 2013

External tray for walk-up:

Together with Agilent‘s EasyAccess software the 1220 Infinity LC with external

tray is a perfect solution for walk-up environments!

Additional detectors:

Almost all 1260/1290 Infinity detectors and SQ-MS can be added!

Page 8

Innovation: 1220 Infinity Mobile LC Solution Affordable, Flexible, Compatible – Everywhere!

June 27, 2013

Instrument Control Framework

• Boost Performance

Complete UHPLC system for

higher resolution and shorter

run times

• Save Time

Bring your laboratory to the

samples

• Reduce Costs

Most affordable system

giving you access to UHPLC

technology

Page 9

1260 Infinity LC

1260/1290 Valve Solutions

1260 Infinity Bio-Inert LC – Buffer Advisor

1260 Infinity SFC

1260 Infinity GPC

1260/1290 ELSD

June 27, 2013

Page 10

1200 Infinity Series valve solutions

Page 11

NEW Quick-Change Valve heads

for universal Valve Drive

1290 Infinity TCC 1290 Infinity Flexible Cube 1290 Infinity Valve Drive

Types 600 bar, 1200bar

Std, Bio-inert, µ-flow, prep

2pos/4port duo

2pos/ 6port

2pos/10port

4pos/10port

6pos/14port

8pos/ 9port

12pos/13port

6pos/ 7port

2pos/ 8port

2pos/12port

Custom Valves

Applications • Multi-method/Method Dev

• Column switching (2,4,6,8)

• Solvent selection (13 – 26)

• Matrix-stripping

• Sample Enrichment

• Online-SPE

• Detector selection

• Heart Cutting 2DLC

• Comprehensive 2DLC

• Alternating Column Regen.

• Process Control/ Sample

Sampling/ Fractionation

• Column Connection

The largest and most flexible valve portfolio Workflow- and Application-Automation Flexibility

July 30, 2013 11

Page 12

Automated Column Regeneration 2-position / 10-port valve head

• 1x 1290 Infinity

Thermostatted column

compartment (or 1290 Infinity

Valve Drive)

• 1x 2-pos/10-port valve head

• While the analysis is running

on column 1, column 2 is

regenerated using the flow

from a second pump

• After the analysis on column

1, the flow switches to

column 2 for analysis while

column 1 is regenerated.

Page 13

Sample Enrichment 2-position / 6-port valve head

• 1x 1290 Infinity

Thermostatted column

compartment (or 1290 Infinity

Valve Drive)

• 1x 2-pos/6-port valve head

• The analyte is retained and

concentrated on an

enrichment column while the

sample matrix flows to

waste.

• For analysis, the

concentrated sample is

flushed out of the

enrichment column to the

analytical column.

Page 14

Sample Cleanup 2-position / 6-port valve head

• 1x 1290 Infinity

Thermostatted column

compartment (or 1290 Infinity

Valve Drive)

• 1x 2-pos/6-port valve head

• Matrix components are

retained on a pre-column

while the analytes pass

through to the analytical

column for separation.

• After sample cleanup or

matrix stripping, the

precolumn is flushed in

reversed flow direction by

the second pump.

Page 15

Column Selection for Method Development 6-position / 14-port valve head

• 1x 1290 Infinity

Thermostatted column

compartment

• 1x 6-pos/14-port valve

head

• Up to 6 columns (4 columns

recommended because of

available space)

Page 16

Solvent Selection for Method Development 12-position selection valve head

Typically used with 1290 Infinity Valve Drive.

For Method Development systems:

1290 Infinity Valve Drive and new 12-position

selection valve head is fully supported

as pump-valve cluster and by Agilent

ChemStation Method Scouting Wizard

Page 17

1260 Infinity Bio-Inert LC

June 27, 2013

Page 18

Innovation: 1260 Infinity Bio-inert LC Truly Bio-inert UHPLC System

June 27, 2013

1260 Infinity Bio-inert LC

• Boost Performance

Highly versatile (U)HPLC

system for small- and large-

molecule analysis

• Save Time

Fast switching between bio-

and non-bio applications,

including cleaning

• Reduce Costs

Only one system to maintain

for bio- and non-bio analysis

Stainless steel system

1260 Infinity Bio-inert LC

Page 19

New Capillary Design Ensures Bio-inertness

June 27, 2013

Capillaries

Metal clad PEEK capillary

Newest fitting design: Hybrid technique

New capillary technolgy enables

600 bar AND is completely metal free !!

Page 20

June 27, 2013

Buffer Advisor Software Facilitates Workflows in Ion Exchange Chromatography

Buffer Advisor helps to:

1. Define stock solutions for salt and pH

gradients

2. Check if the buffer system is suitable

for your separation task

3. Create a corrected time table for a

salt- or pH gradient.

Manual preparation:

1. Tedious

2. Error-prone

3. Irreproducible (operator)

Part of Bio-inert pump,

G5611A

Page 21

Buffer Advisor User Interface

Area 1: Select Buffer and

Gradient Type

June 27, 2013

Area 2: Enter Gradient

Time Table

Area 4: Review

Composition of Stock

Solutions and Adjust

Area 3: Gradient Display

Section: Process

Gradient and Review

Optimized Gradient with

Compensation Steps

Page 22

1260 Infinity SFC

June 27, 2013

Page 23

Innovation: Hybrid SFC/UHPLC System Double your Discovery

June 27, 2013

Hybrid SFC/UHPLC System

• Boost Performance

SFC with LC-like sensitivity,

unique 600 bar x 5 mL/min

power range

• Save Time

Efficient instrument utilization

and orthogonality through

unique switching capability

between LC and SFC

• Reduce Costs

Lower solvent expenses, less

waste, and only one system

to maintain. Upgrade from LC

to SFC

Page 24

Agilent 1260 Infinity Analytical SFC/MS System 1260 Infinity SFC and the G6100 SQ Mass Spectrometer

June 27, 2013

Analysis of leachables in

food packaging material by

SFC/MS

Page 25

1260 Infinity GPC

June 27, 2013

Page 26

High Performance GPC/SEC for Routine Work

June 27, 2013

High Performance GPC/SEC Solution

• Boost Performance

High performance pump & oven

for reproducible molecular

weights

• Save Time

RID warm up less than 1 hour,

complete system based on

industry-leading Agilent LC

systems for maximum uptime

• Reduce Costs

RID with built-in solvent recycle,

minimum training effort.

“I have always been impressed by the

quality, robustness, reliability and ease-

of-use of their products, which have

served me well.” - John P. O’Donnell,

Research Scientist, FUJIFILM Imaging Colorants

Ltd., Manchester, UK

Page 27

1260 Infinity GPC/SEC Multi Detector Suite Summary

June 27, 2013

Key Benefits

Comprehensive Allows complex multi detector GPC

experiments to be performed routinely.

Versatile The MDS can be controlled by software

or the keypad for maximum ease of

use, and can be used with any existing

modular or integrated HPLC system.

Convenient Data from an existing detector can be

collected and processed through the

MDS.

High Quality

Results

The detector cells are heated for

optimum baseline stability and

minimum instrument downtime.

Adaptable Can be expanded at any time to

include additional detectors.

Safe Incorporates multiple solvent leak

detectors to prevent spillage of

potentially hazardous solvents.

Page 28

Agilent 1260 Infinity ELSD

& 1290 Infinity ELSD

Evaporative Light Scattering

Detection (ELSD)

Page 29

Introduction: ELSD General Benefits

June 27, 2013

• Universal detection

• More uniform response

• Gradient Compatible

• Complimentary to LC-MS

• Eliminates derivatization steps in analytical methods

Page 30

Agilent ELSD Portfolio

June 27, 2013

G4260B G4261B

1260 Infinity ELSD

1290 Infinity ELSD

(Cooling deleted)

1290 Infinity ELSD

(Standard, cooled)

Light Source Blue LED (480nm) Blue Laser (405nm)

Temperature

range

Nebulizer: 25-90°C

Evaporator: ambient – 120°C

Nebulizer: 25-90°C

Evaporator: ambient – 120°C

Nebulizer: 25-90°C

Evaporator: 10°C – 80°C

Features

• Hydrocortisone limit of detection

30ng

• RS232 Communication only

• Hydrocortisone limit of detection 4ng

• 5-9 x improvement in sensitivity over current model

• Unique sub-ambient operation (greater response for volatile

compounds)

• RS232 and LAN Communication

• Voltage selection (110/240V) no longer required

• Variable gas flow for uniformity of response

• Fast data collection (up to 80Hz)

• Controlled via ChemStation / EZChrom

• Front Panel control of all parameters (Temperature, gas flow, data rate etc)

Page 31

Increased sensitivity, reduced baseline noise

June 27, 2013

The increased laser intensity

coupled with a high gain

photomultiplier and digital signal

processing increases signal and

reduces noise.

G4261B

S/N 158

G4260B

S/N = 5

Injection: 10µL (Hydrocortisone 50ng-on column)

Column: Zorbax Eclipse XDB-C18 (5µm, 4.6x150mm)

Mobile Phase: Water/ACN (80/20)

Flow Rate: 1.0 m/min

ELSD: Neb 40°C, Evap 60°C, Gas 1.05SLM

Signal/noise calculated using OpenLab Chemstation v1.04

Page 32

Triglycerides in Natural Oils

June 27, 2013

Sample: 2mg/ml Starflower Oil

Column: C18 5µm, 250x4.6mm

Eluent A: ACN

Eluent B: DCM

Gradient: 30-50% B in 40 mins; 50-90% in 2

mins, hold for 3mins

Flow Rate: 1.0ml/min

Inj Vol: 20µl

Detector: neb=40°C, evap=70°C, gas=1.4 SLM

Page 33

Environmental Application:

Characterizing Biodiesel

June 27, 2013

Column: BDS Hypersil Cyano 3µm, 250x4.6 mm

Eluent A: 0.4% Acetic acid in Hexane

Eluent B: 0.4% Acetic acid in Methyl tert-Butyl Ether (MTBE)

Gradient: 100% A, hold 5 mins; 0-80% B in 10 mins,

hold 2 mins; 80-0%B in 3 mins

Flow Rate: 1.0 ml/min

Inj Vol: 20µl

Detector: neb=40°C, evap=80°C, gas=0.8SLM

Page 34

1290 Infinity LC

1290 Infinity Quaternary Pump

1290 2D LC

1290 Infinity with ISET

June 27, 2013

Page 35

1290 Infinity Quaternary LC

June 27, 2013

Page 36

Innovation: 1290 Infinity Quaternary Pump Quaternary Pump with Binary Pump-like Performance

June 27, 2013

1290 Infinity Quaternary Pump

• Boost Performance

Highest, binary-like accuracy

and precision for composition

and flow, with exceptionally low

delay volume

• Save Time

Accelerates method transfer

from R+D to QA and from

HPLC to UHPLC

• Reduce Costs

One pump for any applications:

Most universal – at HPLC-like

costs of ownership

Page 37

Power Range

1200bar up to 2mL/min

800bar at 5mL/min

For any kind of analysis

Flow Accuracy and Precision

< 0.07 % RSD or 0.01 min SD

± 1.0 % or 10 µL

High RT precision in

isocratic runs

Composition Accuracy and Precision

< 0.15 % RSD or 0.02 min SD

± 0.4 % (1-99 % Composition B)

High RT precision in

gradient runs

Agilent 1290 Infinity Quaternary Pump Specifications & Benefits

Composition Range

1-99 %

Wide analytical range

Delay Volume

< 350 µL

For fast quaternary gradients

Page 38

1290 Infinity 2D-LC Solution

June 27, 2013

Page 39

Innovation: 1290 Infinity 2D-LC Solution Square your Separation

June 27, 2013

1290 Infinity 2D-LC Solution

• Boost Performance

New levels of separation power,

detection sensitivity and

precision in 2D-LC

• Save Time

A single ultra high peak

capacity run instead of many

conventional runs – with 1min

method setup!

• Reduce Costs

Special features to reduce

solvent and valve cost, upgrade

paths for existing LC-systems

Page 40

Data Analysis

June 27, 2013

For heart-cutting 2D-LC by Agilent‘s OpenLAB CDS ChemStation ed.

For comprehensive 2D-LC Agilent recommends:

GC Image LCxLC Edition Software from GC Image LLC.

Key features offered:

• Direct Agilent datafile import

• Peak identification, integration, annotation

• Comparative Analysis and Visualization

• Compound-libraries

• MS-data handling

Page 41

Hardware Module-flexibility

June 27, 2013

1. Dimension

2. Dimension

1290 Infinity

Binary Pump

1290 Infinity

Binary Pump

1290 Infinity

Autosampler or 1260

HiP Autosampler

Optional

1260/1290

Infinity

Detector

One or two

1290 Infinity

TCC

Optional

1260/1290

Infinity

Detector

1260/1290

Infinity

detector

1260 Infinity Binary

or Quaternary Pump

1260 Infinity

Capillary Pump 1260 Infinity

Autosampler

For 1st

dimension

chromatogram

and peak-

triggering

To monitor

waste-line

Almost any Agilent pump or autosampler in the 1st dimension!

Almost any detectors are supported!

A 1290 Infinity Binary Pump for the 2nd dimension is required.

Page 42

Applications examples - components of beer samples

June 27, 2013

Separation by RP (C18)

Sepa

ration b

y R

P (

Phe

nyl)

Analysis of two different commercially available beer samples with different

separation modes

1st dimension - Reverse Phase (C18) 2nd dimension - Reverse Phase (Phenyl)

Beer A Beer B

Page 43

1290 Infinity Binary LC w. ISET

Page 44

Innovation: ISET Intelligent System Emulation Technology

June 27, 2013

Intelligent System Emulation Techn.

• Boost Performance

The same retention times

and peak resolution as the

target system

• Save Time

No method redevelopment or

system modification required

• Reduce Costs

Minimized investment and

service costs, with one

system for all methods

Page 45

June 27, 2013

What is Method Transfer? Different Aspects

From particle size to particle size (HPLC to UHPLC)

often associated with a change of instruments due to constraints in max. pressure, extra-

column band broadening etc.. Some fine-tuning of method might be required due to

frictional heating effects

available tools : Agilent Method translator, Third Party Method development SW (ACD

Labs, ChromSword etc.)

From one eluent type or phase chemistry to an other

Method development

available tools: Method development SW (Chromsword, ACD Labs, etc.)

From column dimension to column dimension (e.g. 4.6 to 2.1 mm i.d., 50 mm to 100 mm)

recalculate flow rates, recalculate gradient times, adjust connection capillaries and flow

cells, due to delay volume results may vary, method might need revalidation

available tools: Agilent Method translator

From Instrument to Instrument

with method/instrument change: Isocratic Hold / Pre-injection, Vol. Modification

available tools w/o method or instrument change : none until ISET

Page 46

Intelligent System Emulation Technology Agilent Technical and Application Notes

Public

ation n

um

ber

5990

-9545E

N

Public

ation n

um

ber

5990

-9546E

N

Public

ation n

um

ber

5990-9

703E

N

Public

ation n

um

ber

5990-9

703E

N

Public

ation n

um

ber

5990-9

703E

N

Page 47

Instrument Control Framework

June 27, 2013

Page 48

Innovation: Instrument Control Framework Integrate your Agilent LC with other CDS

June 27, 2013

Instrument Control Framework

• Boost Performance

Full functionality of all Agilent

LC Systems in non-Agilent

data systems

• Save Time

Fast plug-and-play updates

to latest Agilent LC features,

modules, and systems

• Reduce Costs

Access new Agilent features

in non-Agilent software,

without extra coding