Trilogy Development Kit

User’s Manual

Version 1.2January 23, 2015P/N 998-7280

Turner Designs845 W. Maude Ave.Sunnyvale, CA 94085Phone: (408) 749-0994FAX: (408) 749-0998www.turnerdesigns.com

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Table of Contents

1.0 TRILOGY DEVELOPMENT KIT CHECKLIST.......................................................................... 4

2.0 MODULE ASSEMBLY .............................................................................................................. 5

3.0 THEORY OF FLUORESCENCE............................................................................................... 6

4.0 FILTER AND LED SELECTION GUIDE................................................................................... 7

4.1 FILTER CHARACTERISTICS FILTER PART NUMBER 034-0604 ................................................. 84.2 FILTER CHARACTERISTICS FILTER PART NUMBER 034-9430 ................................................. 94.3 FILTER CHARACTERISTICS FILTER PART NUMBER 034-0551 ............................................... 104.4 FILTER CHARACTERISTICS FILTER PART NUMBER 034-9485 ............................................... 114.5 FILTER CHARACTERISTICS FILTER PART NUMBER 034-3700 UG-1...................................... 124.6 SPECTRAL SCAN BLUE LED.............................................................................................. 134.7 SPECTRAL SCAN UV LED.................................................................................................. 144.8 SPECTRAL SCAN YELLOW LED ......................................................................................... 154.9 SPECTRAL SCAN GREEN LED............................................................................................ 16

5.0 CUVETTE SELECTION .......................................................................................................... 17

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1.0 TRILOGY DEVELOPMENT KIT CHECKLIST

Using this list, check all items to ensure the order is complete. This list is for the Development Kit only. The

optional items should be checked against the packing slip received with the shipment. To assist in

identifying items, use the illustration on the next page.

TRILOGY Development KitModel #: 7200-080

Accessories include:

Main Module Housing UV Led Assembly, Turner Designs Part Number: 004-0796 Green Led Assembly, Turner Designs Part Number: 004-0361 Yellow Led Assembly, Turner Designs Part Number: 004-0717 Blue Led Assembly, Turner Designs Part Number: 004-0746 Filter- UG-1, Turner Designs Part Number: 034-3700 Filter 550nm, shortpass, Turner Designs Part Number: 034-0551 Filter 600nm, Turner Designs Part Number: 034-0604 Filter 485nm, shortpass, Turner Designs Part Number: 034-9485 Filter 410-450nm, Turner Designs Part Number: 034-9430 10 Pcs. Filter Gasket, Turner Designs Part Number: 040-2012 4 Pcs, Retainer Ring, Turner Designs Part Number: 056-0145 6 Pcs, Screws, Turner Designs Part Number: 075-0132 LED Spacer, Turner Designs Part Number: 078-0501 Small Phillips Screwdriver, Turner Designs Part Number: 139-0601 Retaining Ring Pliers, Turner Designs Part Number: 139-0602 Tweezers, Turner Designs Part Number: 139-0603 10 PCS, Filter Gasket, Turner Designs Part Number: 040-0320 Filter Aperture, Turner Designs Part Number: 078-7200 Development Kit Users Manual, Turner Designs Part Number 998-7280

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2.0 MODULE ASSEMBLY

1. Using the filter and LED specification sheets contained in this manual, choose theappropriate combination for your application.

2. Use the Tweezers to handle the filters and the gaskets. If the filter that you have selectedhas a mirrored appearance to a specific side, the mirrored-side should face the LED or thesample chamber.

3. Do not use excessive force when you are installing any of the optic components.

4. Use the filter gaskets(040-2012) as necessary to prevent the filter from moving inside themodule housing

*Safety glasses should be worn when installing the retainer rings.

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3.0 THEORY OF FLUORESCENCE

The diagram above illustrates the operation of a filter fluorometer.

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4.0 FILTER AND LED SELECTION GUIDE

#1 Filter 034-0551 390nm to 550nm#2 Filter 034-3700 260nm to 420nm, >680nm#3 Filter 034-9430 410nm to 450nm#4 Filter 034-9485 365nm to 590nm, >770nm#5 Filter 034-0604 410nm to 600nm

250 300 350 400 450 500 550 600 650 700 750

UV LED350nm to 400nm

GREEN LED475nm to 560nm

YELLOW LED590nm to 630nm

BLUE LED430nm to 500nm

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4.1 FILTER CHARACTERISTICS FILTER PART NUMBER 034-0604

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4.2 FILTER CHARACTERISTICS FILTER PART NUMBER 034-9430

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4.3 FILTER CHARACTERISTICS FILTER PART NUMBER 034-0551

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4.4 FILTER CHARACTERISTICS FILTER PART NUMBER 034-9485

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4.5 FILTER CHARACTERISTICS FILTER PART NUMBER 034-3700 UG-1

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4.6 SPECTRAL SCAN BLUE LED

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4.7 SPECTRAL SCAN UV LED

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4.8 SPECTRAL SCAN YELLOW LED

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4.9 SPECTRAL SCAN GREEN LED

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5.0 CUVETTE SELECTION

♦ Polystyrene cuvettes are acceptable to use for fluorophores with wavelengthsbetween 350nm and 800nm.

♦ Methacrylate cuvettes can be used between 300nm and 800nm.

♦ Glass cuvettes may also be used at wavelengths above 300nm.

♦ Quartz cuvettes should be used for wavelengths below 300nm.