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Ways of Studying Cells A. Visualization of Cells SCI 420 Advanced Cell Biology Sept 11, 2002 Any nation that does not honor its heroes will not ndure.” -Abraham Lincoln In honor of those who died 9/11/2001 and those inju n searching the rubble in the aftermath)

Ways of Studying Cells A. Visualization of Cells

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Ways of Studying Cells A. Visualization of Cells. BSCI 420 Advanced Cell Biology Sept 11, 2002 “Any nation that does not honor its heroes will not long endure.” -Abraham Lincoln (In honor of those who died 9/11/2001 and those injured in searching the rubble in the aftermath). - PowerPoint PPT Presentation

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Page 1: Ways of Studying Cells  A. Visualization of Cells

Ways of Studying Cells A. Visualization of Cells

BSCI 420 Advanced Cell Biology Sept 11, 2002

“Any nation that does not honor its heroes will not long endure.” -Abraham Lincoln

(In honor of those who died 9/11/2001 and those injured in searching the rubble in the aftermath)

Page 2: Ways of Studying Cells  A. Visualization of Cells

Resolving Power

Page 3: Ways of Studying Cells  A. Visualization of Cells

Bright-fieldlight microscope

Page 4: Ways of Studying Cells  A. Visualization of Cells

Resolution depends on the Numerical Aperture

Page 5: Ways of Studying Cells  A. Visualization of Cells

Types of LM: A) Bright field B) Phase contrast

Page 6: Ways of Studying Cells  A. Visualization of Cells

Types of LM: C) Diff. Interference Contrast

D) Dark field

Page 7: Ways of Studying Cells  A. Visualization of Cells

Bright field microscopy of stained section of kidney tubules

Page 8: Ways of Studying Cells  A. Visualization of Cells

Bright field vs. Phase contrast

Page 9: Ways of Studying Cells  A. Visualization of Cells

Constructive and destructive interference of light

Page 10: Ways of Studying Cells  A. Visualization of Cells

Fluorescence Microscope

Page 11: Ways of Studying Cells  A. Visualization of Cells

Fluorescence image of a cell in mitosis labeled w 3 tags:Green: fluorescein-labeled anti-tubulin (MTS)Red: anti-centromereproteinBlue: DAPI(DNA)

Page 12: Ways of Studying Cells  A. Visualization of Cells

Indirect Immunocytochemistry

Page 13: Ways of Studying Cells  A. Visualization of Cells

Excitation and Emission wave-lengths

Note emission w-l is always > thanexcitation w-l.So they have lost energy(Energy = 1/wave-length

Page 14: Ways of Studying Cells  A. Visualization of Cells

Immunofluorescence of individual MTs

Page 15: Ways of Studying Cells  A. Visualization of Cells
Page 16: Ways of Studying Cells  A. Visualization of Cells

Images of Conventional (A) vs Confocal (B) Fluorescence

(Drosophila embryo stained w fluorescent probe against actin)

Page 17: Ways of Studying Cells  A. Visualization of Cells

GFT tagged talin in trichrome cells of Arabidopsis

Page 18: Ways of Studying Cells  A. Visualization of Cells

Dynamics of GFP-labeled Cajal bodies in the nucleus