YSMAN067 Anglais rév.1 (Hema Star II service)

Hema Star II

Technical information

Hema Star II S -A / rév.1.0 YSMAN067

2

Summary

SUMMARY 2

ELECTRICITY SUPPLY 3

1. GENERAL REMARKS 3 2. FIRST POWER SUPPLY 3 3. SECOND POWER SUPPLY 3 4. SCREEN 3

DESCRIPTION OF ELECTRONIC BOARDS 4

1. SYSTEM BOARD 4 2. PREAMPLIFIER BOARD L10-CI-PRA20 4 3. PROCESSING BOARD L1O-PR-FIL3O 4 4. MECHANICAL / FLUIDIC MANAGEMENT BOARD L10-CI-DIL30 4 5. INTERFACE BOARD L1O-CI-INT-AA 4

SEQUENCING OF MAIN FLUIDIC CYCLES 5

1. DILUTION SEQUENCING 5 2. COUNTING CYCLE 6 3. RINSING AND DRAINAGE AT THE END OF COUNTING CYCLE (DILUTER) 6 4. RINSING AND DRAINAGE AT THE END OF CYCLE (FLUIDIC) 6 5. SEQUENCING FIRST RINSING CYCLE 7 6. SEQUENCING OF RINSING CYCLE EXCEPT THE FIRST 8 7. SEQUENCING OF RINSING CYCLES AFTER 5 MINUTES 9

LIST OF ELECTRONIC SCHEMES 10

LIST OF FLUIDIC SCHEMES 10


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Electricity supply 1. General remarks The device works on 10w voltage power from two sources in ventilated casing. The main supply element is Iocated at the back of the device preventing any risk of electrocution. Important: These partitioned power supplies do not have any accessible fuses. Contact your technical support. N.B: the presence of the different voltages displayed by leds on the interface board (appendix E3). 2. First power supply PC type power supply giving all boards the following voltages: • +12V • -12V • +5V Power supply linked to the floppy disk driver and the system board, then to all other boards via the expansion slots and cable layers. It works on 50 or 60 Hz and there is a switch between the two EEC jacks for changing to 115 or 230V. 3. Second power supply Works on 30 VI 2 A, feeding the interface board. Accepts from 47 to 440 Hz and 2 voltage ranges, switched over by internal strap or automatic according to the version: • - 95 to 132V • -187 to 265V 4. Screen Works on fixed voltage 220 V, 50 to 60 Hz. On request, it may be supplied with different power supply voltages.


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Description of electronic boards Refer to block diagram : appendix E1. 1. System board Board of 80386 or 80486 type. The program, written in an advanced language, loaded in the core memory after power on. The dialog with the peripheral boards L10-CI-FIL-30 and L10-CI-DIL30 is in mode 1/0 by reciprocal interruption. 2. Preamplifier board L10-CI-PRA20 It polarizes the two counting probes and amplifies the signals. The hemoglobin determination system is on the preamplifier board. 3. Processing board L1O-PR-FIL3O Made up of three structures with independent Z80 microprocessors. Each structure has a specific task : • counting of red corpuscles • platelets • white corpuscles / hemoglobin. The different signals are sorted out on peak width and height. 4. Mechanical / Fluidic management board L10-CI-DIL30 Two Z80 structures deal with the mechanical and fluidic management of the device. They control all the fluidic functions and all the motors following the program of the different cycles. 5. Interface board L1O-CI-INT-AA Power interface board which is linked to the management board by a 60 lead cable layer. Diluter Inputs / Outputs : • needle mechanism • liquid syringe • from electrovalve EV1 to EV3 • start button • red and green leds to start the system up. Fluidics Inputs / Outputs : • volumetric counting sensor and drainage optic sensor • liquid micro-pump • from electrovalve EV4 to EVI 2 • air syringe


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Sequencing of main fluidic cycles 1. Dilution sequencing

PHASE SYRINGE NEEDLE HOLDER EV1 EV2 EV3 BLOOD SAMPLE

Sample – 32 µl Amount – 11 ml

Total ascent

EXT. RINSING OF NEEDLE

3 ml ext. needle Descent 40 mm Init → rinsing û FIRST DILUTION

2 ml ext. needle 2 ml int. needle 1.5 ml ext. needle 1.5 ml int. needle to init

Ascent 15 mm Descent 15 mm Ascent 5 mm Ascent 5 mm

Rinsing → WBC

û

û û

û

û

û û

û DROP EXTRACTOR

Flush elimination

Descent 15 mm Ascent 15 mm

SAMPLE IN DILUTION 1

Sample –43.7 µl Amount –10 ml

Descent 23 mm Ascent 29 mm

EXT. RINSING OF NEEDLE

1.5 ml ext. needle

WBC → rinsing

û

SECOND DILUTION

Descent 9 mm

Rinsing → RBC

REQUEST TRANSFER CHAMBER WBC + LYSIS 6.5 ml ext. needle û û

1.5 ml int. needle û û û RINSING LIQUID IN CHAMBER WC

Amount – 7 ml 7 ml ext. needle

Ascent 5 mm RBC → WBC

û

REQUEST TRANSFER CHAMBER RBC RINSING LIQUID IN CHAMBER RC

Amount – 7 ml 6 ml ext. needle 1 ml int. needle

WBC → RBC

û û

û û

REQUEST COUNTING CYCLE POSITION AWAIT OF COUNTING

To switch Descent 20 mm

RBC → rinsing


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2. Counting cycle

PHASE ACTION FIRST COUNTING CHART Pressure lowering with air syringe

Injection of a bubble into the capillary Bubble passes in front of the start sensor Bubble passes in front of the end sensor Control of counting time Transfer of results WC, RC, PLT to PC

SECOND COUNTING CHART Counting of duration determined by first chart Transfer of results WBC, RBC, PLT to PC

THIRD COUNTING CHART Pressure lowering with air syringe Injection of a bubble into the capillary Bubble passes in front of the start sensor Bubble passes in front of the end sensor Control of counting time Transfer of results WC, RC, PLT to PC

FOURTH COUNTING CHART Counting of duration determined by third chart Hemoglobin measure Transfer of results WBC, RBC, PLT to PC

3. Rinsing and drainage at the end of counting cycle (diluter)

PHASE SYRINGE NEEDLE HOLDER EV1 EV2 EV3 AFTER THE END OF COUNTING

Backlash elimination

Total ascent Descent 75 mm

To slide Slide return Complete return

READY FOR ANOTHER CYCLE

4. Rinsing and drainage at the end of cycle (fluidic)

CHAMBER DIL. WBC CHAMBER DIL. RBC CHAMBER WBC CHAMBER RBC Drainage Transfer

CHAMBER READY Drainage Drainage Transfer

CHAMBER READY Drainage Drainage control Drainage control


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5. Sequencing first rinsing cycle PHASE SYRINGE NEEDLE HOLDER EV1 EV2 EV3

REQUEST CAPILLARY RINSING REQUEST DRAINAGE OF CHAMBERS WBC AND RBC

1st DISTRIBUTION TO RINSING CHAMBER

Amount 9 ml 4 ml ext. needle

Ascent 50 mm Descent 50 mm

Init → rinsing

û

û

REQUEST DRAINAGE OF NEEDLE RINSING CHAMBER 4 ml int. needle û û

REQUEST DRAINAGE OF NEEDLE RINSING CHAMBER 2nd DISTRIBUTION TO RINSING CHAMBER

Amount 9 ml 4 ml ext. needle

û

û

REQUEST DRAINAGE OF NEEDLE RINSING CHAMBER 4 ml int. needle û û û

REQUEST DRAINAGE OF RINSING CHAMBER REQUEST DRAINAGE OF COUNTING CHAMBER WBC

1st DISTRIBUTION TO CHAMBER WBC

Amount 8 ml 5 ml ext. needle 2 ml int. needle


Rinsing → WBC

û û

û REQUEST TRANSFER CHAMBER WBC

REQUEST DRAINAGE OF COUNTING CHAMBER WBC 2nd DISTRIBUTION TO CHAMBER WBC



WBC → RBC

û û

û û

1st DISTRIBUTION TO CHAMBER RBC


û û

û û

REQUEST TRANSFER CHAMBER RBC REQUEST DRAINAGE OF COUNTING CHAMBER RBC

2nd DISTRIBUTION TO CHAMBER RBC


û û

û û

REQUEST TRANSFER CHAMBER RBC REQUEST DRAINAGE OF COUNTING CHAMBER RBC

3rd DISTRIBUTION TO CHAMBER WBC

Amount 9 ml

REQUEST TRANSFER CHAMBER WBC + LYSIS 6 ml ext. needle

2 ml int. needle û

û

û û

û û

REQUEST TRANSFER CHAMBER RBC REQUEST COUNTING CYCLE


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6. Sequencing of rinsing cycle except the first PHASE SYRINGE NEEDLE HOLDER EV1 EV2 EV3


1st DISTRIBUTION TO RINSING CHAMBER

Amount 5 ml 2 ml ext. needle 2 ml ext. needle


Init → rinsing

û û

û û

REQUEST DRAINAGE OF NEEDLE RINSING CHAMBER DISTRIBUTION TO CHAMBER WBC



Rinsing → WBC

û û

û û

DISTRIBUTION TO CHAMBER RBC

Amount 9 ml


WBC → RBC

REQUEST TRANSFER CHAMBER WBC 6 ml ext. needle

2 ml int. needle û

û

û û

û û

REQUEST TRANSFER CHAMBER RBC REQUEST COUNTING CYCLE


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7. Sequencing of rinsing cycles after 5 minutes PHASE SYRINGE NEEDLE HOLDER EV1 EV2 EV3


1st DISTRIBUTION TO CHAMBER WBC

Amount 8 ml 5 ml ext. needle 2 ml ext. needle


Init → WBC

û û

û û

REQUEST TRANSFER CHAMBER WBC 2nd DISTRIBUTION TO CHAMBER WBC

Amount 8 ml

û

REQUEST DRAINAGE OF CHAMBER WBC 5 ml ext. needle

2 ml int. needle û

û

û û

DISTRIBUTION TO CHAMBER RBC

Amount 8 ml Ascent 30 mm Descent 30 mm

WBC → RBC

REQUEST TRANSFER CHAMBER WBC 5 ml ext. needle

2 ml int. needle û

û

û û

û û

REQUEST TRANSFER CHAMBER RBC REQUEST CAPILLARY RINSING REQUEST CAPILLARY RINSING MECHANICAL INITIALIZATIONS


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List of electronic schemes Appendix D1 : Preamplifier board L10-CI-PRA20 Appendix D2 : A/D conversion board L10-ANA20 Appendix D21 : Component location on conversion board Appendix D3 : Processing board L10-CI-FIL30 Appendix D31 : Component location on processing board Appendix D4 : Mechanical / Fluidic management board L10-CI-DIL30 Appendix D41 : Component location on mechanical / fluidic management

board Appendix D5 : Interface board L10-CI-INT-AA Appendix D51 : Component location on interface board Appendix E1 : Machine block diagram Appendix E22 : Trimmer location on preamplifier board Appendix E3 : Trimmer location on interface board Appendix E44 : Trimmer location on A/D conversion board

List of fluidic schemes Appendix F1 : Fluidic cabling Appendix F2 : Fluidic skeleton diagram Appendix F21 : WBC dilution transfer Appendix F22 : RBC dilution transfer Appendix F23 : Volumetric sensor rinsing (detergent circuit) Appendix F24 : Counting Appendix F25 : Drainage of counting chambers Appendix F26 : Diluter sequences Appendix F3 : Dismounting of ruby apertures Appendix F4 : Air and liquid syringe greasing


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Appendix D1

PREAMPLIFIER BOARD L10-CI-PRA20


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Appendix D2

A/D CONVERSION BOARD L10-ANA20


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Appendix D21

COMPONENT LOCATION ON CONVERSION BOARD


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Appendix D3

PROCESSING BOARD L10-CI-FIL30


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Appendix D31

COMPONENT LOCATION ON PROCESSING BOARD


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Appendix D4

MECHANICAL / FLUIDIC MANAGEMENT BOARD L10-CI-DIL30


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Appendix D41

COMPONENT LOCATION ON MECHANICAL / FLUIDIC MANAGEMENT BOARD


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Appendix D5

INTERFACE BOARD L10-CI-INT-AA


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Appendix D51

COMPONENT LOCATION ON INTERFACE BOARD


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Appendix E1

MACHINE BLOCK DIAGRAM


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Appendix E22

TRIMMER LOCATION ON PREAMPLIFIER BOARD


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Appendix E3

TRIMMER LOCATION ON INTERFACE BOARD


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Appendix E44

TRIMMER LOCATION ON A/D CONVERSION BOARD


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Appendix F1

FLUIDIC CABLING


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Appendix F2

FLUIDIC SKELETON DIAGRAM


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Appendix F21

WBC DILUTION TRANSFER

Tubes concernés EV contrôlées x


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Appendix F22

RBC DILUTION TRANSFER



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Appendix F23

VOLUMETRIC SENSOR RINSING (DETERGENT CIRCUIT)



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Appendix F24

COUNTING



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Appendix F25

DRAINAGE OF COUNTING CHAMBERS



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Appendix F26

DILUTER SEQUENCES

Needle external distribution Needle internal distribution

Blood or 2nd

dilution extraction Diluent loading

Appendix F3

DISMOUNTING OF RUBY APERTURES

Rubis S -A / rév.2

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Appendix F4

AIR AND LIQUID SYRINGE GREASING

Documents

YSMAN067 Anglais rév.1 (Hema Star II service)