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Page 1: High Efficiency Electroporation

FOR TECHNICAL SUPPORT | Tel +1.608.441.2852 | www.TheTransfectionExperts.com

Ingenio® Electroporation Solution & Kits

Save on Cost Without Compromising Your Results Product Cost/Electroporation* Savings

Amaxa® Nucleofector® Kit V (VCA-1003) -

Ingenio® Electroporation Kit (MIR 50112) 42% *Based on list prices from company websites and protocol recommendations (25 RXN Kit).

Figure 1. Cells were electroporated in parallel with an EGFP reporter vector and assayed at 24 hours post-electroporation by flow cytometry. Two electroporators were used with different electroporation solutions: the Ingenio® Electroporation Kit was used in the Gene Pulser Xcell™ Eukaryotic System (Bio-Rad) or the Amaxa Nucleofector II Device (Lonza); the Amaxa Nucleofector Kit V was used in the Amaxa Nucleofector II Device, all according to manufacturers’ recommendations.

Ingenio Provides Comparable Efficiency on Amaxa’s Nucleofector Device

Prove it to Yourself −Try a FREE Sample of Ingenio Solution Today!

High Efficiency Electroporationx Save money and reduce research costs while maximizing results

x Compatible with conventional electroporation devices including Lonza-Amaxa®, Bio-Rad® or Harvard BTX®

x Ideal for plasmid or siRNA electroporation

x High efficiency electroporation of hard to transfect cell lines and primary cells

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% E

GFP

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ells

HL-60 K-562 Jurkat E6-1 SK-N-MC

Ingenio® Solution using Gene Pulser

Ingenio® Solution using Amaxa® Nucleofector®

Amaxa® Nucleofector® Solution V on Amaxa® Nucleofector®

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Page 2: High Efficiency Electroporation

FOR TECHNICAL SUPPORT | Tel +1.608.441.2852 | www.TheTransfectionExperts.com

www.TheTransfectionExperts.com

Ingenio® Electroporation Kits (solution, 0.2 cm cuvettes, cell droppers)

PRODUCT NO. SIZE

MIR 50112 25 RXNa

MIR 50115 50 RXNa

MIR 50118 100 RXNa

Ingenio® Electroporation Kits (solution, 0.4 cm cuvettes, cell droppers)

PRODUCT NO. SIZE

MIR 50113 25 RXNa

MIR 50116 50 RXNa

MIR 50119 100 RXNa

*Primary cell types

Figure 2. Ingenio® Electroporation Kits were used to transfect representative cell types using Amaxa® Nucleofector®. Cells were assayed at 24 hours post-electroporation by flow cytometry and reported as percentage of live cell population. Visit www.TheTransfectionExperts.com for ideal pulse conditions.

©2011 All rights reserved. Mirus Bio LLC. All trademarks are the property of their respective owners.FLING-09/2011

Efficient Plasmid DNA Delivery into Any Cell Type Including Primary Cells

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K562

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SK-N

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*MEF

Ingenio® Electroporation SolutionPRODUCT NO. SIZE

MIR 50111 25 RXNa

MIR 50114 50 RXNa

MIR 50117 100 RXNa

Cuvettes and cell droppers also sold separately.

a Number of electroporations per kit.

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