Research Fellowship in

Human Reproduction and Male Infertility

Our Research Fellowship and training opportunities represent one of the most active, state-of-the-art and comprehensive programs in human reproduction and infertility in the United States.

Our Fellows gain priceless research experience under the strong mentorship of world-renowned experts in reproductive medicine and biology at our Center in the Cleveland Clinic.

Center for Reproductive Medicine

Scientific Presentation Day, Summer Internship 2014, Lerner Research Institute

For more information, please contact:

Center for Reproductive Medicine and the Andrology CenterCleveland Clinic, X-1110681 Carnegie AvenueCleveland OH 44195, United StatesTel: +1.216.444.9485Toll-Free: 1.800.CCF.CARE (ext. 4-9485)Fax: +1.216.445.6049Email: CRM@CCF.ORGhttp://www.ClevelandClinic.org/ReproductiveResearchCenter

Table of ContentsOur Mission 2Testimonials 2-3Application 3Who We Are 4Books Published 4Research Collaborators 5, 8, 9Research Overview 6 – 14Publications 8 – 14Recent Research Alumni 15 – 16Fulbright Research Scholars 17About Our Center 18 – 19Reproductive Research Alumni 20 – 22About Cleveland 23

Testimonials from Research Alumni:

“I sincerely believe that the Center for Reproductive Medicine at the Cleveland Clinic helped me accomplish my short-term and long-term goals. I would highly recommend this program for anyone who has a desire to learn and become successful in the world of academic medicine.”

Deepinder Goyal, MD | 2006 – 07

“The training that I received at your Program has made a profound impact on my professional career. My achievements in the field of male infertility and andrology have to be credited to the opportunity of being a Fellow at CRM. I truly believe that this is the best research fellowship program in reproductive medicine currently available both in the United States and abroad. The program not only met but exceeded its goals in every single aspect. It was a wonderful

professional and personal experience for life.”

Sandro Esteves, MD, PhD | 1995 – 96

“My memories of the Cleveland Clinic are of being pushed to work harder and to never accept mediocre quality of work. This program teaches one to set high goals, and to become better at everything one does.”

Fabio Pasqualotto, MD, PhD | 1998 – 99

“I was extremely fortunate to work with renowned expertsin the field of Reproductive Medicine from the United States,Germany, and the U.K. as a part of their collaborationwith the Center for Reproductive Medicine. The innovativeresearch that we produced remains a cornerstone in theareas of sperm sorting and sperm genomic integrity. I was able to publish over 30 articles in high-impact Reproductive Medicine

Journals as well as book chapters. In addition, I was provided with the opportunity to attend several international conferences during which I presented over 60 oral and poster presentations.”

Tamer Said, MD, PhD | 2002 – 06

“The program inculcates a great deal of ability to do hard work, punctuality, sincerity, art of communication with professionals, feeling of responsibility and aptitude to work as team-member in fellows. The fellows will learn how to do multiple tasks simultaneously and efficiently.”

Shyam Allamaneni, MD | 2003 – 04

“I was impressed by the systematic environment in the laboratory. Although I was unfamiliar with bench research at first, I was soon able to focus on my project without hesitation … The experiences in the lab gave me a strong belief that if I had an idea, I could make anything possible in that setting.”

Won Jun Choi, MD, PhD | 2004 – 05

“Once I joined as Post Doctoral Research Fellow under the leadership of Dr. Agarwal at CCF, it was the beginning of the new era in my academic career. And when I look back, it was the stay at CCF which molded me or I should say prepared me as a Scientist in Biomedical field. Even after my tenure was over at CCF, I am continuing as an academic collaborator with CRM, which has resulted in many collaborative publications in

journals and as books. The program offers several learning opportunities in the form of class room teachings every week by experts from multidisciplinary units of the CCF, a rich diversity of clinical cases, multitude of opportunities in both clinical as well as basic research, interaction with students from across the globe, an easy access to latest literature, expert research guides and many more. It was really one of my best experiences in life. I do thank each and every member of CRM who have dedicated themselves for the betterment of biomedical research and thereby quality outcome through the ones who are trained at CRM and are now serving the people in many countries.”

Alex C. Varghese, PhD | 2007 – 08

“The lab at CRM was like my second home where every single moment spent is like a golden memory. “Once a member of CRM, always a member of CRM”. Any fellow who joins the CRM develops an eternal bond with the department. The huge amount of work I’ve learnt here is an invaluable asset for all times to come. Thank you CRM for believing in my capabilities and letting me accomplish all my dreams. I’m going back home successful and with a wide smile on my face.”

Arozia Moazzam, MD, PhD | 2010 – 11

Our MissionOur unique and methodical mentorship approach develops our researchers into independent and dynamic scientists of the highest caliber.

• Personal mentorship approach with daily supervision• Practical demonstration and hands-on training in laboratory techniques • Weekly research meetings for project planning and development• On-site and online courses on various research procedures• Training in literature review strategies and tools• Training in writing of scientific articles for publication• Lectures and presentations• Journal Club and group discussions

• Receive personalized mentoring by well-known experts in Reproductive Research• Receive intensive hands-on training to master various basic and advanced research skills• Learn the basics of Good Laboratory Practices• Learn how to conduct a thorough literature review/ critical analysis of published research• Develop superior communication and presentation skills• Participate actively in cutting-edge reproductive research• Develop a strong foundation in human reproduction, assisted reproduction and infertility• Learn the process of developing a novel scientific research idea• Learn the techniques of writing a scientific bench research proposal• Master the art of writing high quality scientific articles • Publish research findings in top-tier scientific journals• Learn the fundamentals of research involving human subjects• Learn the basics of data management and biostatistical analysis• Learn to be an independent, confident and resourceful researcher

Research Fellowship BrochurePublished on: Feb 20, 2015

2 | Cleveland Clinic Center for Reproductive Medicine

Our Research Fellows will

Develop Valuable Research Skills

• Scientific integrity and accountability• Professionalism and leadership• Discipline and time management• Motivation and self-direction• Team work and collaboration• Creativity and innovation• Organization and methodical planning• Effective interpersonal communication• Critical thinking and judgement• Problem solving and trouble shooting

Research &Training Methods

Who can apply?Medical graduates, physicians, and scientists interested in conducting cutting-edge bench research in the field of Reproductive Medicine are welcome to apply. Applicants are selected on a competitive basis from a pool of candidates from around the world. Once approved, the candidate is appointed as a Research Fellow through the Cleveland Clinic’s Graduate Medical Education Program.

“I consider my experience to be successful. I am very happy with the outcome, given the unique circumstances of having a limited amount of time to spare for research due to my heavy clinical responsibilities.”

John McGill, MD | 2012 – 13

Research Fellowship BrochurePublished on: Feb 20, 2015

3 | Cleveland Clinic Center for Reproductive Medicine

When applying online, you will need:

Recent curriculum vitae/resume

3 recent letters of recommendation from persons with first-hand knowledge of your work

Copies of degrees/certificates, transcripts and TOEFL score

Copy of passport and a passport-sized photo

Proof of sponsorship/scholarship

Address, telephone number, and Skype ID for a personal interview with the Director

A limited number of Fellowship positions are available every year. Only the most qualified applicants with a genuine desire to learn and engage in real research will be considered. International candidates (scientists/physician researchers) are welcome to apply. Open call application process (no specific deadline). For more information on applying for the 2015 Research Fellowship, please click here.

“I have benefited tremendously from the time, effort, thought and care that went into my training. The one-on-one mentorship and guidance was personalized to suit my strengths and weaknesses, and this truly made the difference. The great emphasis placed on personal growth made this a unique experience with a life-long impact.”

Damayanthi Durairajanayagam, PhD | 2012 – 13

“This Fellowship has revealed my strengths and weaknesses - situations in which I shine and moments in which I flame out. Besides a valuable lesson on how to work hard and smart, I have learnt which features of mine I have to work on in order to become a better scientist as well as person.”

Eva Tvrda, MSc (PhD student) | 2013 – 14

“I gained a lot of knowledge and skills in the field of male infertility. The year was very exciting and went by very fast. Honestly, I would not have gained this level of knowledge anywhere else in a year’s duration. My deepest gratitude to Dr Agarwal for being a great mentor and a real friend. I truly appreciate and value everything I have learned from Dr Agarwal and his team. It will remain a major contributor behind my success and achievements.”

Saad Alshahrani, MD | 2012 – 13

“I came to CRM looking for interaction with new research and to expand my ideas and knowledge. After a year, I have achieved my goals. I’m very thankful for all that Dr Agarwal, Dr Sharma and Dr Gupta have done for me, the teaching and the sharing of experiences. The program is of great value if you are looking to grow as a person and as a professional.”

Helena Malvezzi, MSc | 2012 – 13

“Throughout my training, I received strong support from my mentors. They provided me with systematic training, very good suggestions for improvement and prompt feedback. The Fellows worked as a team and supported each other in all our work. I will never forget my experience here in CRM.”

Cui Zhihong, MD | 2013 – 14

“I am sure that in no other lab in the world can a Fellow accomplish such a huge amount of work in such a short time. The Andrology Center is our family in the US and I always felt Dr. Agarwal’s support and encouragement starting from the first day.”

Ahmet Ayaz, MSc (PhD student) | 2013 – 14

Testimonials from Research Alumni:

How to apply

Apply NOW!

“My overall experience was good. I gained a lot of experience and friends. I would like to thank Dr. Agarwal and CRM for everything they have done for me, it was a great experience - I learned a lot on a professional as well as a personal level.”

Amani Shaman, MD | 2011 – 12

TestimonialsTestimonials

Testimonials

“I sincerely thank CRM for providing me with the chance to pursue my research project. I am also thankful to Dr. Sharma for his generous support and for taking the time to share his expertise and knowledge on my project. The training in the Andrology laboratory procedures was well organized and very satisfactory. The ART Training was very valuable to develop technical expertise in ART techniques. The highly qualified and experienced trainers made the training more beneficial. The 2014

Summer Internship Course was a great teaching experience to be a part of different training activities.”

Sezgin Gunes, PhD | 2014

Who We Are

Sajal Gupta, MDAssistant Professor, Lerner College of MedicineAssistant Research Coordinator,Center for Reproductive Medicine

Rakesh Sharma, PhDAssociate Professor, Lerner College of MedicineResearch Coordinator,Center for Reproductive Medicine

Edmund Sabanegh, MDChairman, Department of UrologyHead Section of Male InfertilityProfessor, Lerner College of Medicine

Ashok Agarwal, PhD, HCLDDirector, Center for Reproductive MedicineDirector, Andrology CenterProfessor, Lerner College of Medicine

Research Fellowship BrochurePublished on: Feb 20, 2015

4 | Cleveland Clinic Center for Reproductive Medicine

• Serve as a Mentor/Advisor during the Summer Mentorship program in Reproductive Medicine held annually in June and July. Click here to read more about our Mentorship Program.

• Receive systematic and comprehensive hands-on ART Training completely free of charge during the Advanced Reproductive Techniques course held annually in Sept and Oct. Fellows who successfully complete their ART Training will receive a Certificate. Click here to read more about our ART Training Program.

Antioxidants in Male Infertility: A Guide for Clinicians and Researchers

Sijo Parekattil, Ashok Agarwal (Editors)Publication Date: November 2013

Table of Contents

Unexplained InfertilityGlenn L. Schattman, Sandro C. Esteves,

Ashok Agarwal (Editors)Publication Date: 2015

Table of Contents

Non-Invasive Sperm Selection for In Vitro Fertilization: Novel Concepts and Methods

Ashok Agarwal, Edson Borges Jr., Amanda S. Setti (Editors)Publication Date: 2015

Table of Contents

Male Infertility: A Complete Guide to Lifestyle and Environmental Factors

Stefan S. du Plessis, Ashok Agarwal, Edmund S. Sabanegh Jr. (Editors)

Publication Date: 2014Table of Contents

Strategies to Ameliorate Oxidative Stress During Assisted Reproduction

Ashok Agarwal, Damayanthi Durairajanayagam,Gurpriya Virk, G., Stefan Du Plessis (Editors)

Publication Date: 2015Table of Contents

Understanding Male Infertility Global Practices and Indian Perspective

Sonia Malik, Ashok Agarwal (Editors)Publication Date: 2014

Table of Contents

Building and Managing an IVFLaboratory: A Practical Guide

Zsolt Peter Nagy,Alex C. Varghese, Ashok Agarwal (Editors)

Publication Date: October 2013Table of Contents

Clinical Embryology: A Practical GuideZsolt Peter Nagy,

Alex C. Varghese, Ashok Agarwal (Editors)Publication Date: October 2013

Table of Contents

Gamete Assessment, Selection and Micromanipulation in ART

Zsolt Peter Nagy,Alex C. Varghese, Ashok Agarwal (Editors)

Publication Date: October 2013Table of Contents

Male Infertility for the Clinician:A Practical Guide

Sijo J. Parekattil, Ashok Agarwal (Editors)Publication Date: August 2013

Table of Contents

Medical and Surgical Managementof Male Infertility

Botros RMB Rizk, Nabil Aziz, Ashok Agarwal, Edmund Sabanegh (Editors)

Publication Date: September 2013 Table of Contents

Sperm Chromatin for the Researcher: A Practical Guide

Armand Zini, Ashok Agarwal (Editors)Publication Date: September 2013

Table of Contents

Male Infertility: Contemporary Clinical Approaches, Andrology, ART

& AntioxidantsSijo J. Parekattil, Ashok Agarwal (Editors)

Publication Date: June 7, 2012Table of Contents

Practical Manual of In Vitro Fertilization: Advanced Methods and Novel Devices

Zsolt Peter Nagy, Alex C. Varghese,Ashok Agarwal (Editors)

Publication Date: April 24, 2012Table of Contents

Studies on Men’s Health and Fertility (Oxidative Stress in Applied Basic Research and Clinical Practice)

Ashok Agarwal, Robert John Aitken,Juan G. Alvarez (Editors)

Publication Date: March 17, 2012Table of Contents

A Workbook on Human Spermatozoa and Assisted Conception

Sonia Malik, Ashok Agarwal (Editors)Publication Date: February 2012

Table of Contents

Fertility Preservation: Emerging Technologies and Clinical Applications

Emre Seli, Ashok Agarwal (Editors)Publication Date: September 23, 2011

Table of Contents

Sperm Chromatin: Biological and Clinical Applications in Male Infertility

and Assisted ReproductionArmand Zini, Ashok Agarwal (Editors)

Publication Date: August 4, 2011Table of Contents

Andrology Laboratory ManualKamini A. Rao, Ashok Agarwal,

MS Srinivas (Editors)Publication Date: February 10, 2010

Table of Contents

Fertility Preservation in Males: Emerging Technologies and

Clinical ApplicationsEmre Seli, Ashok Agarwal (Editors)Publication Date: October 5, 2012

Table of Contents

Fertility Preservation in Females: Emerging Technologies and

Clinical ApplicationsEmre Seli, Ashok Agarwal (Editors)Publication Date: October 5, 2012

Table of Contents

Studies on Women’s Health(Oxidative Stress in Applied Basic Research and Clinical Practice)

Ashok Agarwal, Nabil Aziz, Botros Rizk (Editors)

Publication Date: August 1, 2012Table of Contents

Quality Management in ART Clinics: A Practical Guide

Fabiola Bento, Sandro Esteves,Ashok Agarwal (Editors)

Publication Date: November 13, 2012Table of Contents

Sperm Chromatin for the Clinician: A Practical Guide

Armand Zini, Ashok Agarwal (Editors)Publication Date: August 2013

Table of Contents

During their Fellowship year at CRM, our Research Fellows receive value added opportunities to participate in and...

Dr. Eric Klein (Chairman, Glickman Urological and Kidney Institute), Dr. Edmund Sabanegh and Dr. Ashok Agarwal

ASHOK AGARWAL, Ph.D. Professor, Department of Urology Director, Center for Reproductive Medicine Director, Andrology Center

RAKESH SHARMA, Ph.D.Associate Professor, Department of Urology

Coordinator of ResearchCenter for Reproductive Medicine

SAJAL GUPTA, M.D.Assistant Professor, Department of Urology

Assistant Research CoordinatorCenter for Reproductive Medicine

Glickman Urological and Kidney Institute

· C

ente

r for

ReproductiveM

edicine ·

Cleveland Clinic

The Cleveland Clinic

Eva Tvrda, MSchas served as a Mentor on the Original Bench Research Project entitled

“Spermatozoa Protein Examination in Testicular Cancer Patients”

conducted for 120 hours during the 2014 Summer Internship Program at the Center for Reproductive Medicine June 9 – July 25, 2014

Let it be known that

BOOKS PUBLISHED

The Center for Reproductive Medicine has collaborative relationships with many highly esteemed scientists and clinicians from over

18 countries around the world. Some of our renowned collaborators include:

Research Collaborators & International Faculty

Research Fellowship BrochurePublished on: Feb 20, 2015

5 | Cleveland Clinic Center for Reproductive Medicine

Diana Vaamonde, PhD Cordoba, Spain

Jaime Gosálvez, PhD Madrid, Spain

Mourad Assidi, PhD Jeddah, Saudi Arabia

Rajender Singh, PhD Lucknow, India

Muhammad Abu-Elmagd, PhD Jeddah, Saudi Arabia

Mohamed Arafa, MD, PhD Doha, Qatar

Sandro Esteves, MD, PhD Campinas, Brazil

Stefan du Plessis, PhD Tygerberg, S. Africa

Damayanthi Durairajanayagam, PhDKuala Lumpur, Malaysia

Haitham Elbardisi, MD Doha, Qatar

Sonja Grunewald, MD Liepzig, Germany

Giancarlo Balercia, MD Ancona, Italy

Nabil Aziz, FRCOG, MDLiverpool, United Kingdom

Rima Dada, MD, PhDNew Delhi, India

Sheryl Homa, PhD London, United Kingdom

Laura Sirot, PhDWooster, Ohio

Peter Nagy, MD, PhD Atlanta, Georgia

Ramadan Abdou Saleh, MDSohag, Egypt

Botros Rizk, MD, PhD Mobile, Alabama

Sijo Parekattil, MDClermont, Florida

Philip Kumanov, MD Sofia, Bulgaria

Suresh C Sikka, PhDNew Orleans, Louisiana

Juan Alvarez, MD, PhDBarcelona, Spain

Dolores Lamb, PhDHouston, Texas

Walter Cardona Maya, PhDMedellín, Colombia

John Aitken, PhD, DScNewcastle, Australia

Armand Zini, MD, FRCS(C) Montreal, Canada

Applicants should keep in mind that:

• All appointments for Research Fellowship are for a minimum of 1 year and there is no financial support available.

• Candidates must have independent funds such as a private or government scholarship to support living expenses in the United States.

• Research appointments do not result in the award of a degree; successful completion of training results in the award of a Certificate of Research Training from CRM.

• Candidates registered with their parent institutions/medical schools for a Master’s/PhD/MD degree can select the Cleveland Clinic for their research studies requirements. Research findings, once completed in our Center, can be submitted towards fulfillment of a degree from the candidate’s own institution.

Uwe Paasch, MD, PhD Liepzig, Germany

Other Important Information

Anthony J Thomas Jr, MD | Retired Urologist, Head, Section of Male Infertility, Cleveland Clinic

Research Overview

Research Fellowship BrochurePublished on: Feb 20, 2015

6 | Cleveland Clinic Center for Reproductive Medicine

cAMP Responsive Element Modulator (CREM) signaling in the testis.

Venn diagram showing distribution of 20 differentially expressed proteins. This was based on the NSC (normalized spectral counts) ratio cut-off >2 across 3 samples: NA (normal sperm count and abnormal morphology), OA (oligozoospermia and abnormal morphology), and ON (oligozoospermia and normal morphology) in comparison to the baseline NN (normal sperm count and normal morphology) sample.

Oxidative Stress and InfertilityThe foremost goal of the Cleveland Clinic’s Center for Reproductive Medicine is to better understand the causes of male infertility, to design studies aimed at improving semen quality, and to comprehend the underlying mechanism of male infertility associated with various clinical etiologies. Over the last 2 decades (1993 – 2014), CRM has established itself as a leading laboratory in human infertility research, and particularly in the field of oxidative stress and infertility. CRM Faculty and Researchers have published over 200 key articles in this field and is dedicated to disseminating its results. Many of these studies continue to be cited today. For a publication and citation report from the Scopus database, please click here.

Proteomics – Our Current Research FocusWhile semen analysis remains a cornerstone of laboratory investigation for male infertility, a routine semen analysis alone does not provide information on the underlying molecular alterations in the seminal ejaculates of infertile men. Oxidative stress can affect sperm function and result in modification of proteins in the spermatozoa.

Proteomics involves careful analysis of proteins expressed by a cell or tissue during a particular given state. The study of protein expression has been the subject of intense research for many diseases during the past decade, with much interest devoted to reproductive implications. At present, more than 6,000 discrete proteins have been identified in semen, which represents about three quarters of the entire sperm proteome.

Proteins are of significant importance in cellular remodeling events and aberrant expression could lead to marked defects in sperm function. In cases of those diagnosed with male infertility, differential proteomics may be utilized to study the alteration in protein expression of either the spermatozoa or seminal plasma of these men.

The goal of our proteomics studies is to identify proteins that may be altered or differentially expressed in a given patient population that can serve as potential biomarkers in the etiology of infertility.

Why Proteomics? Examination of sperm function through proteomics will improve on conventional semen analysis in the workup of male infertility.

Transcriptional regulatory network showing interactions between differentially expressed ROS + proteins and androgen receptor.

Identification of sperm specific proteins via proteomics would provide a further understanding of their function pertaining to fertility in the male.

The developing field of sperm proteomics has the further advance potential to our knowledge of the numerous cellular pathways necessary for sperm function and to help identify those with the greatest biological significance in men suffering from infertility.

Proteomics holds the key to the development of novel diagnostic and prognostic protein biomarkers for the evaluation of infertile men by comparing the differential expression of sperm and seminal plasma proteins in fertile vs. infertile men.

The combined application of proteomics and bioinformatics tools can identify major alterations in the proteins involved in various clinical diagnosis of male infertility.

♦

♦

♦

♦

♦

The program continues to provide cutting edge knowledge, experience and appreciation for research with professionalism, integrity and humility.

Kim Dao Ly, BS, MBA, Final Yr Med Student, USAAlumni, 2010

Research Overview

Research Fellowship BrochurePublished on: Feb 20, 2015

7 | Cleveland Clinic Center for Reproductive Medicine

Biochemical pathway proposed to regulate sperm capacitation and hyperactivation.

Intrinsic and extrinsic pathways of apoptosis of the hyperthermic spermatozoa.

How Proteomics Studies Work• Proteins in spermatozoa or seminal plasma samples are separated into peptides, and their expression quantified and subsequently identified using gel electrophoresis followed by a liquid chromatography – tandem mass spectrometry approach.

• Further identification of proteins separated by LC-MS/MS can be achieved using Mascot and Sequest programs. Furthermore, differentially affected processes, pathways and cellular distribution as well as protein protein interactions can be identified via the use of available functional bioinformatics analysis such as Gene Ontology (GO annotations and proprietary software

packages such as Ingenuity Pathway Analysis (IPA).

• The potential proteins that have been identified as differentially expressed are further validated by Western Blot, ELISA or immunohistochemistry to identify the biomarker status of the proteins in various pathological conditions attributed to oxidative stress or in patients with other etiologies.

• Differentially expressed proteins present in infertile men with a particular diagnosis that are involved in sperm function, sperm motility and other functions related to reproduction may serve as novel biomarkers in the identification of that certain disease.

• These biomarkers may help urologists identify better options for clinical management of infertile men.

Extraction and analysis process of proteins from spermatozoa.

Proteomics and Beyond Our recent proteomics research has demonstrated differential protein expression over controls in a variety of situations that include idiopathic male infertility, varicocele, azoospermia and assisted reproductive technology failure. This early work allows the development of methods to study male infertility, which was previously believed to be idiopathic, and ultimately to stratify interventions based on laboratory results.

A list of our recent proteomic publications is featured on pages 8-9. Through these preliminary studies, we have established a platform to utilize proteomic tools to unravel the underlying

mechanisms of these etiologies of male infertility. Results of these proteomics studies could eventually lead to the identification of appropriate antioxidant therapy to alleviate oxidative stress-related infertility.

While we continue to rely on conventional semen parameters in the evaluation of the subfertile male, proteomic analysis holds great promise as a diagnostic tool in the

reproductive medicine armamentarium. With the identification of novel biomarkers through proteomic studies, clinical tests and treatments for sperm dysfunction may be developed to potentially help infertile couples.

2014 Key Publications from CRM

In addition to proteomics studies, a selection of highly citedarticles and other recent important studies published

by our Center is featured on pages 10-14.

For a comprehensive list of our research publicationsin the ResearchGate database, please click here.

♦

♦

♦

♦

♦

I truly appreciate the opportunity to participate in such an excellent research program and to have so many life changing experiences with such incredible people.

Sejal Doshi, BS, Final Yr Med Student, USAAlumni 2012, 2013

Research Overview

Research Fellowship BrochurePublished on: Feb 20, 2015

8 | Cleveland Clinic Center for Reproductive Medicine

Banu Gopalan, PhDDirector of Bioinformatics Consulting

Services, Yorg Corporation Plano, Texas

Jeff Hammel, MSSenior Biostatistician

Cleveland, Ohio

Amy Moore, BAManager, Medical Editing Services,

Education Institute Cleveland Clinic

Selected Research on Proteomics and Infertility Published by Our Center

du Plessis SS, Kashou AH, Benjamin DJ, Yadav SP, Agarwal A.Reprod Biol Endocrinol. 2011 Mar 22; 9:36. doi: 10.1186/1477-7827-9-36. Review.PMID: 21426553 IF: 2.41

Sharma R, Agarwal A, Mohanty G, Jesudasan R, Gopalan B, Willard B, Yadav SP, Sabanegh E.Reprod Biol Endocrinol. 2013 May 11; 11:38.doi: 10.1186/1477-7827-11-38.PMID: 23663294 IF: 2.41

Thacker S, Yadav SP, Sharma RK, Kashou A, Willard B, Zhang D, Agarwal A.Fertil Steril. 2011 Jun 30; 95(8):2745-8. doi: 10.1016/j.fertnstert.2011.03.112. Review.PMID: 21536282 IF: 4.295

Sharma R, Agarwal A, Mohanty G, Hamada AJ, Gopalan B, Willard B, Yadav S, du Plessis S.Reprod Biol Endocrinol. 2013 May 20; 11:48. doi: 10.1186/1477-7827-11-48.PMID: 23688036 IF: 2.41

Sharma R, Agarwal A, Mohanty G, Du Plessis SS, Gopalan B, Willard B, Yadav SP, Sabanegh E.Reprod Biol Endocrinol. 2013 Sep 3; 11:85. doi: 10.1186/1477-7827-11-85.PMID: 24004880 IF: 2.41

Hamada A, Sharma R, du Plessis SS, Willard B, Yadav SP, Sabanegh E, Agarwal A.Fertil Steril. 2013 Apr; 99(5):1216-1226.e2. doi: 10.1016/j.fertnstert.2012.11.046.PMID: 23312230 IF: 4.295

Dheepa Balasubramanian, PhDMolecular Geneticist

Case Western Reserve UniversityCleveland, Ohio

Agarwal A, Durairajanayagam D, Halabi J, Peng J, Vazquez-Levin M.Reprod Biomed Online. 2014 Jul; 29(1):32-58. doi: 10.1016/j.rbmo.2014.02.013. Review.PMID: 24813754 IF: 2.682

Gupta S, Ghulmiyyah J, Sharma R, Halabi J, Agarwal A.Biomed Res Int. 2014; 2014:916212. doi: 10.1155/2014/916212. Review.PMID: 24900998 IF: 2.706

REVIEW

Proteomics, oxidative stress and maleinfertility

Ashok Agarwal a,*, Damayanthi Durairajanayagam a,b, Jacques Halabi a,Jason Peng a, Monica Vazquez-Levin c

a Center for Reproductive Medicine, Glickman Urological and Kidney Institute, Cleveland, OH, USA; b Faculty of Medicine,MARA University of Technology, Sungai Buloh, Selangor, Malaysia; c Institute of Biology and Experimental Medicine,National Research Council of Argentina, CONICET, Buenos Aires, Argentina* Corresponding author. E-mail address: agarwaa@ccf.org (A Agarwal).

Ashok Agarwal is a Professor at the Lerner College of Medicine, Case Western Reserve University and the Head ofthe Andrology Center and Director of Research at the Center for Reproductive Medicine, Cleveland Clinic, USA.He has researched extensively on oxidative stress and its implications on human fertility. He serves on theeditorial boards of several key journals in human reproduction. Ashok’s current research interests are the studyof molecular markers of oxidative stress, DNA fragmentation and apoptosis using proteomics and bioinformaticstools, as well as fertility preservation in patients with cancer, and the efficacy of certain antioxidants inimproving male fertility.

Abstract Oxidative stress has been established as one of the main causes of male infertility and has been implicated in many dis-eases associated with infertile men. It results from high concentrations of free radicals and suppressed antioxidant potential, whichmay alter protein expression in seminal plasma and/or spermatozoa. In recent years, proteomic analyses have been performed tocharacterize the protein profiles of seminal ejaculate from men with different clinical conditions, such as high oxidative stress. Theaim of the present review is to summarize current findings on proteomic studies performed in men with high oxidative stress com-pared with those with physiological concentrations of free radicals, to better understand the aetiology of oxidative stress-inducedmale infertility. Each of these studies has suggested candidate biomarkers of oxidative stress, among them are DJ-1, PIP, lactotrans-ferrin and peroxiredoxin. Changes in protein concentrations in seminal plasma samples with oxidative stress conditions were relatedto stress responses and to regulatory pathways, while alterations in sperm proteins were mostly associated to metabolic responses(carbohydrate metabolism) and stress responses. Future studies should include assessment of post-translational modifications in the

spermatozoa as well as in seminal plasma proteomes of men diagnosed with idiopathic infertility. RBMOnline

ª 2014, Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.

KEYWORDS: biomarkers, male infertility, proteomics, oxidative stress, seminal plasma, spermatozoa

http://dx.doi.org/10.1016/j.rbmo.2014.02.0131472-6483/ª 2014, Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.

Reproductive BioMedicine Online (2014) 29, 32–58

www.sc iencedi rec t . comwww.rbmonl ine .com

This certificate is presented to:

For her work as “Research Fellow” in Andrology/Urology

the Center for Reproductive Medicine, Cleveland Clinic

March 19, 2013 – March 19, 2014

The 12 month fellowship includes: participation in original bench research, writing of scientific articles

and public presentation of research findings.

In witness whereof, this certificate is awarded this 19 th day of March, 2014.

ASHOK AGARWAL, Ph.D., HCLD

Professor, Department of Urology

Director, Center for Reproductive Medicine &

Director, Andrology Laboratory RAKESH SHARMA, Ph.D.

Associate Professor, Department of Urology

Coordinator of Research

Center for Reproductive MedicineSAJAL GUPTA, M.D.

Assistant Professor, Department of Urology

Assistant Research Coordinator

Center for Reproductive Medicine

Zhihong Cui, MDChongqing, China

The Cleveland Clinic

· C

ente

r for

Reproductive Medicine ·Cleveland Clinic

Glickman Urological and Kidney Institute

1

5

2

6

3

7

4

8

Belinda Willard, PhDDirector, Proteomics CoreLerner Research Institute

Our Research Collaborators

The combination of things I learned at the program truly makes it a unique program. It is multi-faceted and thus a valuable asset to any career path.

Julia Tsinberg, BS, USA Alumni 2014

Research Overview

Research Fellowship BrochurePublished on: Feb 20, 2015

9 | Cleveland Clinic Center for Reproductive Medicine

Barazani Y, Agarwal A, Sabanegh ES Jr.Urology. 2014 Aug; 84(2):255-61. doi: 10.1016/j.urology.2014.04.043. Review.PMID: 25065986 IF: 2.132

DamayanthiDurairajanayagam, PhDKuala Lumpur, Malaysia

Eva Tvrda, PhD Nitra, Slovak Republic

Cui Zhihong, MDChongqing, China

Ahmet Ayaz, PhDIstanbul, Turkey

Selected Research on Proteomics and Infertility Published by Our Center

Saad Alshahrani, MD Al-Kharj, Saudi Arabia

Sezgin Gunes, PhD Samsun, Turkey

Sandro Esteves, MD, PhDCampinas, Brazil

Reecha Sharma, MD, MSPhiladelphia, PA

Tamer M Said, MD, PhDToronto, Canada

Arozia Moazzam, MD, PhD Lahore, Pakistan

Alaa Hamada, MDBoston, MA

Ramadan Abdou Saleh, MDSohag, Egypt

Alex Varghese, PhDKerala, India

Rachel Jesudasan, PhDHyderabad, India

9

ARTICLESin Press 1

Differential proteomic profiling of spermatozoa proteins of infertile

men with unilateral or bilateral varicocele. Agarwal A, Sharma R, Durairajanayagam D, Ayaz A,

Cui Z, Willarb B, Gopalan B, Sabanegh E.Urology (In Press) IF=2.132

Collaborating Research Alumni

3Spermatozoa protein alterations in

infertile men with bilateral varicocele.Agarwal A, Sharma R, Durairajanayagam D, Ayaz A,

Cui Z, Willarb B, Gopalan B, Sabanegh E.Asian Journal of Andrology (In Press)

IF=2.530

2Major protein alterations in

spermatozoa from infertile men with unilateral varicocele.

Agarwal A, Sharma R, Durairajanayagam D,Ayaz A, Cui Z, Willarb B, Gopalan B, Sabanegh E.

Reproductive Biology and Endocrinology (In Press)IF=2.41

4Proteomic analysis of mature

and immature ejaculatedspermatozoa from fertile men.

Cui Z, Sharma R, Agarwal A.Asian Journal of Andrology (Under Review)

IF=2.530

5Impact of precise modulation of

reactive oxygen species levels on spermatozoa proteins in infertile men.

Ayaz A, Agarwal A, Sharma R,Arafa M, Elbardisi H, Cui Z.

Clinical Proteomics (In Press)IF=3.43

To read more about our collaborators, please click here

Meet Our Research Staff

Rakesh Sharma, PhD | Associate Professor and Research Coordinator

Sajal Gupta, MD, MS, TS (ABB) | Assistant Professor and Assistant Research Coordinator

Rakesh Sharma is an Associate Professor at the Lerner College of Medicine of Case Western Reserve University and is the Coordinator of the Andrology Center and the Center for Reproductive Medicine. Dr. Sharma has published over 200 scientific papers in peer-reviewed scientific journals, authored 30 book chapters, and presented over 360 abstracts at both national and international scientific meetings. He is an investigator on 55 research grants. Dr. Sharma is a recipient of the American Foundation for Urologic Disease (AFUD) Fellow Award, Merlyn F. Bumpus Junior Investigator Award, Research Excellence Award, Research Fellow of the Year Award, Mentor Recognition Award, Scientist of the Year Award, Excellence in Male Infertility Research Award and the 2012 Star Award from the American Society for Reproductive Medicine. Dr. Sharma’s current research interests include the role of free radicals in the pathophysiology of male and female infertility, oxidative stress and DNA integrity, alterations in oxidative stress-related proteins, sperm proteomics apoptosis, fertility preservation and endometriosis-associated infertility.

Sajal Gupta is an Assistant Professor of Surgery at the Lerner College of Medicine of Case Western Reserve University and is the Supervisor of the Andrology Center and Assistant Coordinator of Research at the Center for Reproductive Medicine. She obtained a Masters in Clinical Embryology and Andrology from The Jones Institute for Reproductive Medicine in Virginia. Dr. Gupta has published over 60 reviews and research articles in peer-reviewed scientific journals, authored a dozen book chapters and presented about 55 abstracts at both national and international scientific meetings. She is an investigator on 18 research grants. Dr. Gupta is a recipient of the Award for Highest Productivity in Female Infertility Research, Research Fellow of the Year Award, and Award for Excellence in Female Infertility Research. Her current research interests include the role of free radicals in male and female infertility, endometriosis, assisted reproductive techniques and gamete cryobiology.

sharmar@ccf.org+1.216.444.8182

guptas2@ccf.org+1.216.444.8182

This has been one of the most significant experiences of my life not only for the knowledge and professional advancement it helped facilitate but also for the great times I had with the staff and my peers.

Stephen Bowen, BS, USA Alumni 2013

Research Overview

Research Fellowship BrochurePublished on: Feb 20, 2015

10 | Cleveland Clinic Center for Reproductive Medicine

20 Most Cited Articles Published by Our Center

Agarwal A, Saleh RA, Bedaiwy MAFertil Steril. 2003 Apr; 79(4):829-43.PMID: 12749418 Citation count: 801

Saleh RA, Agarwal AJ Androl. 2002 Nov-Dec; 23(6):737-52.PMID: 12399514 Citation count: 374

Sharma RK, Agarwal AUrology. 1996 Dec; 48(6):835-50. Review.PMID: 8973665 Citation count: 656

Agarwal A, Makker K, Sharma RAm J Reprod Immunol. 2008 Jan; 59(1):2-11. Review.PMID: 18154591 Citation count: 375

Agarwal A, Gupta S, Sharma RKReprod Biol Endocrinol 2005 Jul 14; 3(28):1-21. Review.PMID: 16018814 Citation count: 636

Bedaiwy MA, Falcone T, Sharma RK, Goldberg JM, Attaran M, Nelson DR, Agarwal A Hum Reprod.2002 Feb; 17(2):426-31.PMID: 11821289 Citation count: 331

Agarwal A, Said TMHuman Reproduction Update 2003 Jul-Aug; 9(4):331-45.PMID: 12926527 Citation count: 509

Agarwal A, Nallella KP, Allamaneni SSR, Said TMReprod Biomed Online. 2004 Jun; 8(6):616-27. Review. PMID: 15169573 Citation count: 309

1

5

2

6

3

7

4

8

Fellows in Andrology with Highest Research Productivity

Tamer M. Said, MD, PhDToronto, CanadaAlumni 2002-06

tamersaid@repromedltd.comPublications with CRM: 28*

Sandro C. Esteves, MD, PhDCampinas, BrazilAlumni 1995-96

s.esteves@androfert.com.brPublications with CRM: 23*

Fabio F. Pasqualotto, MD, PhDCaxias do Sul, Brazil

Alumni 1998-99fabio@conception-rs.com.brPublications with CRM: 19*

Reda Z. Mahfouz, MD, PhDCleveland, Ohio, USAAlumni 2006-2010mahfour@ccf.org

Publications with CRM: 19*

1 2 3 4

Jorge Hallak, MD, PhDSão Paulo, BrazilAlumni 1996-98

hallakj@androscience.com.brPublications with CRM: 18*

Shyam Sunder Rao Allamaneni, MDCincinnati, Ohio, USA

Alumni 2003-04shyamraoa@yahoo.com

Publications with CRM: 18*

5 6

*These publications include only peer-reviewed, PubMed-indexed research articles that were either published during the Fellow’s stint at Cleveland Clinic or subsequentlyin collaboration with CRM Faculty (search date Jan 30, 2015). For a comprehensive list of publications of these alumni with CRM, click here.

The experience at CRM taught me to think analytically and solve problems quickly, while encouraging me to present new ideas to a consistently receptive and welcoming research team.

Aditi Mulgund, BS, MPH, Final Yr Med Student, USA Alumni 2013-2014

Research Overview

Research Fellowship BrochurePublished on: Feb 20, 2015

11 | Cleveland Clinic Center for Reproductive Medicine

Hendin BN, Kolettis PN, Sharma RK, Thomas AJ Jr, Agarwal AJ Urol. 1999 Jun; 161(6):1831-4.PMID: 10332447 Citation count: 290

Pasqualotto FF, Sharma RK, Nelson DR, Thomas AJ Jr, Agarwal A Fertil Steril. 2000 Mar; 73(3):459-64.PMID: 10688996 Citation count: 278

Naughton CK, Nangia AK, Agarwal AHum Reprod Update. 2001 Sep-Oct; 7(5):473-81.PMID: 11556494 Citation count: 283

Moustafa MH, Sharma RK, Thornton J, Mascha E,Abdel-Hafez MA, Thomas AJ Jr, Agarwal A Hum Reprod. 2004 Jan; 19(1):129-38. PMID: 14688171 Citation count: 268

Sharma RK, Pasqualotto FF, Nelson DR, Thomas AJ, Agarwal AHum Reprod. 1999 Nov; 14(11):2801-7.PMID: 10548626 Citation count: 280

Agarwal A, Said TMBJU Int. 2005 Mar; 95(4):503-7.PMID: 15705068 Citation count: 266

Saleh RA, Agarwal A, Nada EA, El-Tonsy MH, Sharma RK, Meyer A, Nelson DR, Thomas AJ Fertil Steril. 2003 Jun; 79 Suppl 3:1597-605. PMID: 12801566 Citation count: 277

Ollero M, Gil-Guzman E, Lopez MC, Sharma RK, Agarwal A, Larson K, Evenson D, Thomas AJ Jr, Alvarez JG. Hum Reprod. 2001 Sep; 16(9):1912-21. PMID: 11527898 Citation count: 261

20 Most Cited Articles from Our Center

13

9

14

10

15

11

16

12

Fellows in Andrology with Highest Research Productivity

*These publications include only peer-reviewed, PubMed-indexed research articles that were either published during the Fellow’s stint at Cleveland Clinic or subsequentlyin collaboration with CRM Faculty (search date Jan 30, 2015). For a comprehensive list of publications of these alumni with CRM, click here.

Ramadan Abdou Saleh, MDSohag, Egypt

Alumni 1999-2002salehr2010@yahoo.com

Publications with CRM: 17*

7

Alaa Hamada, MDBoston, MA, USAAlumni 2010-12

ala1977hh@gmail.comPublications with CRM: 14*

8 9 10 11 12

Alex C. Varghese, PhDKerala, India

Alumni 2007-08 alex@lifeinvitro.com

Publications with CRM: 12*

Kiran P. Nallella, MDWarangal, IndiaAlumni 2003-05

nkiran_prasad@yahoo.comPublications with CRM: 10*

Nisarg R. Desai, MDAlton, Illinois, USAAlumni 2007-08

nisargdesai1@yahoo.comPublications with CRM: 10*

Marcello Cocuzza, MD, PhDSão Paulo, BrazilAlumni 2006-07

mcocuzza@uol.com.brPublications with CRM: 10*

I have learned so many things from my research experience which I have incorporated into my life and my studies. It has made me into a better person.

Wedad Al-Najjar, Medical Student, Saudi Arabia Alumni 2014

Research Overview

Research Fellowship BrochurePublished on: Feb 20, 2015

12 | Cleveland Clinic Center for Reproductive Medicine

Gil-Guzman E, Ollero M, Lopez MC, Sharma RK, Alvarez JG, Thomas AJ, Agarwal A. Hum Reprod. 2001 Sep; 16(9):1922-30. PMID: 11527899 Citation count: 250

Agarwal A, Saleh RAUrol Clin North Am. 2002 Nov; 29(4):817-27.PMID: 12516754 Citation count: 250

Saleh RA, Agarwal A, Sharma RK, Nelson DR, Thomas AJ Jr Fertil Steril. 2002 Sep; 78(3):491-9.PMID: 12215323 Citation count: 248

X Wang, RK Sharma, SC Sikka, AJ Thomas Jr, T Falcone, A Agarwal Fertil Steril. 2003 Sep; 80(3):531-5.PMID: 12969693 Citation count: 243

20 Most Cited Articles from Our Center

17 18 19 20

Certificate of Training Award Ceremony

One of our medical technologist during testing

Andrology Open House, April 2014

The Accuri BD Flow Cytometer for testing DNA damage

The research program at CRM is very unique and offers diverse research experiences. I grew both professionally and personally under the CRM Staff’s mentorship.

Reecha Sharma, MD, MPH, USA Alumni 2010-2011

Research Overview

Research Fellowship BrochurePublished on: Feb 20, 2015

13 | Cleveland Clinic Center for Reproductive Medicine

Selected Recent Publications from CRM

Agarwal A, Mulgund A, Alshahrani S, Assidi M, Abuzenadah AM, Sharma R, Sabanegh E. Reprod Biol Endocrinol. 2014 Dec 19;12(1):126. IF: 2.132

Reactive oxygen and nitrogen species generation under

heat and hypoxic stress in infertile men with varicocele

Durairajanayagam D, Agarwal A, Ong C, Prashast P.Asian J Androl. 2014 May-Jun;16(3):420-5. doi: 10.4103/1008-682X.126384. PMID: 24675655 IF: 2.530

CREM (cAMP responsive element modulator) signaling in the testis

Cross sectional view of testicular tissue showing various

effects of cell phone radiofrequency-electromagnetic

wave (RF-EMW) on cellular components of the testis

Tahmasbpour E, Balasubramanian D, Agarwal A.J Assist Reprod Genet. 2014 Sep;31(9):1115-37. doi: 10.1007/s10815-014-0280-6. PMID: 25117645 IF: 1.772

Ko EY, Sabanegh ES Jr, Agarwal A. Fertil Steril. 2014 Dec; 102(6):1518-1527. doi: 10.1016/j.fertnstert.2014.10.020. PMID: 25458618 IF: 4.295

Agarwal A, Durairajanayagam D, du Plessis SS. Reprod Biol Endocrinol. 2014 Nov 24; 12:112. doi: 10.1186/1477-7827-12-112. PMID: 25421286 IF: 2.132

Agarwal A, Durairajanayagam D. Asian J Androl. 2014 Nov 18. doi: 10.4103/1008-682X.140963.PMID: 25432495 IF: 2.530

Sharma R, Kattoor AJ, Ghulmiyyah J, Agarwal A.Syst Biol Reprod Med. 2014 Oct 29:1-12.PMID: 25354153 IF: 1.85

Hamada A, Esteves SC, Agarwal A.Nat Rev Urol. 2013 Jan;10(1):26-37. doi: 10.1038/nrurol.2012.198. PMID: 23165400 IF: 4.522

1

5

2

6 7

4

8

3

Basal lamina

Spermatogonia

Leydig cells

Cytoskeletal damage,dislocated nucleus

Damaged plasmamembranes

Irregular germ cells

Contracted, ovoidshape

2011

Sertoli nucleus

Junctional complex

Damagedjunctional complex

Antibodies

Reactive OxygenSpecies (ROS)

Porousplasmamembrane

Junctionalcomplexdisruption

DNAbreaks

Research Overview

Research Fellowship BrochurePublished on: Feb 20, 2015

14 | Cleveland Clinic Center for Reproductive Medicine

Agarwal A, Sharma RK, Sharma R, Assidi M, Abuzenadah AM, Alshahrani S, Durairajanayagam D, Sabanegh E. Reprod Biol Endocrinol. 2014 May 7; 12:33. doi: 10.1186/1477-7827-12-33. PMID: 24885775 IF: 2.132

Agarwal A, Tvrda E, Sharma R. Reprod Biol Endocrinol. 2014 May 27; 12:45. doi: 10.1186/1477-7827-12-45.PMID: 24884815 IF: 2.132

Agarwal A, Mulgund A, Sharma R, Sabanegh E. Syst BiolReprod Med. 2014 Aug; 60(4):206-16. doi:10.3109/19396368.2014.918675. PMID: 24815996 IF: 1.85

Agarwal A, Virk G, Ong C, du Plessis SS. World J Mens Health. 2014 Apr; 32(1):1-17. doi: 10.5534/wjmh.2014.32.1.1. PMID: 24872947

pISSN: 2287-4208 / eISSN: 2287-4690

World J Mens Health 2014 April 32(1): 1-17http://dx.doi.org/10.5534/wjmh.2014.32.1.1 Review Article

Received: Oct 14, 2013; Accepted: Oct 24, 2013

Correspondence to: Ashok Agarwal

Center for Reproductive Medicine & Andrology Center, Glickman Urological and Kidney Institute, Cleveland Clinic, Desk

X11, 10681 Carnegie Avenue, Cleveland, Ohio 44195, USA.

Tel: +1-216-444-9485, Fax: +1-216-445-6049, E-mail: agarwaa@ccf.org

Copyright © 2014 Korean Society for Sexual Medicine and AndrologyThis is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons. org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Effect of Oxidative Stress on Male Reproduction

Ashok Agarwal1, Gurpriya Virk1, Chloe Ong1, Stefan S du Plessis2

1Center for Reproductive Medicine, Cleveland Clinic, Cleveland, OH, USA, 2Medical Physiology, Faculty of Medicine and Health Sciences, Stellenbosch University, Tygerberg, South Africa

Infertility affects approximately 15% of couples trying to conceive, and a male factor contributes to roughly half of these cases.

Oxidative stress (OS) has been identified as one of the many mediators of male infertility by causing sperm dysfunction. OS is a

state related to increased cellular damage triggered by oxygen and oxygen-derived free radicals known as reactive oxygen species

(ROS). During this process, augmented production of ROS overwhelms the body’s antioxidant defenses. While small amounts

of ROS are required for normal sperm functioning, disproportionate levels can negatively impact the quality of spermatozoa and

impair their overall fertilizing capacity. OS has been identified as an area of great attention because ROS and their metabolites

can attack DNA, lipids, and proteins; alter enzymatic systems; produce irreparable alterations; cause cell death; and ultimately,

lead to a decline in the semen parameters associated with male infertility. This review highlights the mechanisms of ROS

production, the physiological and pathophysiological roles of ROS in relation to the male reproductive system, and recent

advances in diagnostic methods; it also explores the benefits of using antioxidants in a clinical setting.

Key Words: Antioxidants; Infertility, male; Oxidative stress; Reactive oxygen species; Spermatozoa

INTRODUCTION

　For most couples, procreating is a natural part of life that involves neither special planning nor intervention. Unfortunately, when trying to conceive, 15% to 25% of the couples struggle and, consequently, seek medical ad-vice on how to improve their chances of fertilization and successful pregnancy [1]. According to the World Health Organization [2] guidelines, in approximately half of these cases, the male factor is the cause of infertility when an “alteration in sperm concentration, motility, and/or mor-phology is present in at least one sample of two sperm

analyses, collected 1 to 4 weeks apart” [3]. This problem is further compounded when no identifiable reason can be found. Currently, oxidative stress (OS) is believed to be an important and plausible cause of idiopathic male infertility.　“OS is a condition that reflects an imbalance between the systemic manifestation of reactive oxygen species (ROS) and a biological system’s ability to readily detoxify (antioxidant defenses) the reactive interm1ediates or to re-pair the resulting damage” [4,5]. In a healthy body, pro-ox-idants and antioxidants remain in balance. Spermatozoa are equipped with antioxidant defense mechanisms and

Selected Recent Publications from CRM

9 10 11 12

For more information on our Summer Mentorship Program, please click here.

NextGen® Home Banking Sperm Kit: Outcomes of Offsite versus Onsite Collection – PreliminaryFinding. Agarwal A, Sharma R, Gupta S,Sharma RK. Urology (In Press)

Our Research Innovation

2012

- 20

1320

13 -

2014

2012

- 20

1320

13 -

2014

Recent Researchers

Research Fellowship BrochurePublished on: Feb 20, 2015

15 | Cleveland Clinic Center for Reproductive Medicine

Helena Malvezzi is an Embryologist at the Sírio Libanês (Syrian-Lebanese) Hospital, São Paulo, Brazil since 2013. She completed her Masters in Biology of Reproduction in 2011 from the University of São Paulo and did a Specialization in Human Reproduction at the Sapientiae Institute, São

Paulo. Helena was a Research Fellow from Jan 2012-Jan 2013 in CRM. In one of her studies at Cleveland Clinic, Helena studied the sperm quality after density gradient centrifugation with three commercially available sperm wash media. Her results showed that the extent of DNA damage in these media was comparable, but sperm processed using two of the media had better motility.

Dr. Saad Alshahrani is an Assistant Professor in Urology at the Salman Bin Abdulaziz University, Saudi Arabia. Saad was a Research Fellow in Andrology at the Center for Reproductive Medicine from Aug 2012 to Jul 2013. He is the Vice Dean for Postgraduate Studies

and Continuous Medical Education in Andrology in Cleveland Clinic. Saad’s main interest is in Andrology. In one of his studies, at Cleveland Clinic, Saad examined the effect of paternal age on semen quality in infertile men. His results showed that advanced paternal age increases the risk of sperm DNA damage.

Ahmet Ayaz is a PhD candidate at the Department of Bioengineering, Yildiz Technical University, Istanbul. Ahmet was a Research Fellow in Andrology at the Center for Reproductive Medicine from May’ 2013 to Aug’ 2014. He came to Cleveland Clinic

on a scholarship from the Scientific and Technological Research Council of Turkey. Ahmet studied the effect of increasing presence of ROS in the seminal ejaculate on the human spermatozoa proteome in male infertility patients (in press, Clinical Proteomics). The goal of his unique study at Cleveland Clinic was to understand the impact of precise modulation of ROS level on sperm protein alterations and biological functions on signaling pathways.

Helena Malvezzi, MScSão Paulo, Brazil

Alumni 2012-2013helenamalvezzi@yahoo.com.br

Saad Alshahrani, MDAl-Kharj, Saudi Arabia

Alumni 2012-2013saadas1@hotmail.com

Ahmet Ayaz, PhDIstanbul, Turkey

Alumni 2013-2014ahmetayaz@gmail.com

2010

- 20

11

2010

- 20

12

Dr. Arozia Moazzam is an Assistant Professor at the Center for Research in Molecular Medicine, University of Lahore, Lahore, Pakistan. She graduated from King Edward Medical College, Pakistan in 1996 and obtained her Ph.D. from the Quaid-i-Azam University,

Islamabad, Pakistan. She was a Research Fellow in the Reproductive Endocrine Unit of Massachusetts General Hospital/Harvard Medical School from 2008 to 2009. She was a Research Fellow in Andrology/Urology at the Center for Reproductive Medicine from February 2010 to January 2011.

Arozia Moazzam, MD, PhDLahore, Pakistan

Alumni 2010-2011drarozia@yahoo.com

Dr. Alaa Hamada is a Clinical Fellow in Robotic and Laparoscopic Uro-oncology at St. Elizabeth Medical Center, Tufts University School of Medicine in the Brighton neighborhood of Boston, MA. He graduated from Al-Nahrain

Teaching Hospital in Baghdad. He was an Observer in Urology at Cleveland Clinic from October 2010 to December 2011. He was appointed as a Research Fellow in Andrology in the Center for Reproductive Medicine in March, 2012.

Alaa Hamada, MDBoston, MA

Alumni 2010-2012ala1977hh@gmail.com

Dr. Cui Zhihong is an Associate Professor in the Institute of Toxicology, Third Military Medical University, Chongqing, China. Zhihong was a Research Fellow in Andrology at the Center for Reproductive Medicine fromMarch 2013

to March 2014. Her research interests are to study the association between environmental exposure to organic pollutants and sperm quality. The goal of one of her studies at Cleveland Clinic was to identify differentially expressed proteins in immature and mature ejaculated spermatozoa in fertile men after density gradient separation (in press, RBMO). Her results provide important information about the proteins critical to spermatozoa maturation, motility and fertilization capacity.

Cui Zhihong, MDChongqing, ChinaAlumni 2013-2014

zhihongcui_4@126.com

I believe that CRM has one of the best and most famous research programs in the world. It is the top place to build a career in reproductive medicine.

Hussein Abdelrazik MD, TS (ABB), MD, Egypt Alumni 2006-2008

Recent Researchers

Research Fellowship BrochurePublished on: Feb 20, 2015

16 | Cleveland Clinic Center for Reproductive Medicine

2014

2014

- 20

15

2014

- 20

15

Dr. Sezgin Gunes received her PhD in Medical Biology and Genetics from the Faculty of Medicine, Ondokuz Mayis University, Turkey where she is currently an Associate Professor of Medical Biology. Sezgin was a Research Fellow in Andrology at the Center for Reproductive Medicine from June to Nov 2014. During her stay in CRM, Sezgin was involved in a bench research study on “Methylation Profile of Catalase (CAT) Gene Promoter in

Oligozoospermic Infertile Males Exhibiting Oxidative Stress”. Her recent research from Cleveland was recently accepted Spermatogenesis, DNA Damage and DNA Repair Mechanisms in Male Infertility. Gunes S, Alsadaanasan M, Agarwal A. Reproductive BioMedicine Online, (accepted).

Dr. Avraham Harlev is a Senior Physician in the Fertility and IVF Unit, Soroka University Medical Center, Israel. He is a Lecturer in the Faculty of Medicine and Health Sciences, Ben-Gurion University of the Negev, Beer Sheva, Israel, from where he graduated as an MD in 2004. Avi is currently a Research Fellow in Andrology at the Center for Reproductive Medicine since Sept 2014 till Sept 2015. Avi is currently conducting advanced

research in male infertility and andrology at CRM. Avi is also researching the role of smoking on male infertility, using an evidence based approach. He has been invited to submit this article to Nature Urology.

Dr. Luna Samanta is a Professor and Head of the Department of Zoology, Ravenshaw University, Cuttack, Orissa, India. Luna recently completed a Commonwealth Academic Staff Fellowship 2012 at the Imperial College of London, United Kingdom. Luna was then awarded a Raman Fellowship for Post Doctorate Research to do advanced research at the Center for Reproductive Medicine, and is currently a Research Fellow in Andrology at our Center from Dec 2014 till Dec 2015. Luna’s

current research interests include research in proteomics and male infertility. She is validating the proteomic results by studying the localization and levels of expression of candidate proteins in spermatozoa and seminal plasma of varicocele patients. These results will be used as biomarkers of pathophysiology.

Sezgin Gunes, PhDSamsun, Turkey

Alumni 2014sgunes@omu.edu.tr

Avraham Harlev, MDBeer Sheva, Israel

Current Fellow 2014-2015harleva@ccf.org

Luna Samanta, PhDOrissa, India

Current Fellow 2014-2015samantl@ccf.org

Interns and Faculty at the 2008 Summer Internship Gala Dinner

and Award Ceremony

Interns and Faculty at the 2012 Summer Internship Gala Dinner and Award Ceremony

Interns and Faculty at the 2010 Summer Internship Gala

Dinner and Award Ceremony

I will always remember Dr Agarwal’s words: “Nothing is impossible in this world. There is always a way.” I repeat his motto in my mind when faced with challenges - this greatly increased my confidence and helped me overcome every hurdle.

Nilopher Surti, MBBS, MD, India Alumni 2008

Fulbright Research Scholars

Research Fellowship BrochurePublished on: Feb 20, 2015

17 | Cleveland Clinic Center for Reproductive Medicine

DamayanthiDurairajanayagam, PhDKuala Lumpur, Malaysia

Alumni 2012-2013damayanthi.d@gmail.com

Stefan du Plessis, PhD, MBATygerberg, South Africa

Current Scholar 2014-2015dupless@ccf.org

Eva Tvrda, PhDNitra, Slovak Republic

Alumni 2013-2014evina.tvrda@gmail.com

Gulfam Ahmad, MCE, MRD, DIU, PhD

Lahore, PakistanCurrent Scholar 2014-2015

ahmadg@ccf.org

Dr. Damayanthi Durairajanayagam is a Senior Lecturer in Physiology at the Faculty of Medicine, MARA University of Technology, Malaysia. Dama received a Fulbright Research Scholar Award to conduct advanced research at the Center for Reproductive Medicine, Cleveland Clinic. Dama was a Research Fellow in Andrology at CRM from Sept 2012 to Sept 2013. Dama’s research at Cleveland Clinic examined the sperm protein profiles of infertile men with either unilateral or bilateral varicocele. Results (in press) of these prospective studies could help identify the differential pathways that lead to infertility based on the uni- or bilateral presence of varicocele in these men. By identifying key proteins involved in the pathogenesis of varicocele, researchers may acquire an insight into the mechanism with which varicocele affects sperm quality, and eventually help make a significant impact in improving semen parameters and pregnancy rates in these men.

Dr. Stefan du Plessis is a Professor and Head of the Division of Medical Physiology, Faculty of Medicine and Health Sciences, at Stellenbosch University, South Africa. Stefan heads the Stellenbosch University Reproductive Research Group in the Department of Biomedical Sciences, responsible for basic research on male gametes and post graduate student training. He has published more than 50 peer reviewed scientific articles. Twenty of these articles were published in collaboration with the Cleveland Clinic. Stefan received a Fulbright Research Scholar Award to conduct advanced research at the Center for Reproductive Medicine, Cleveland Clinic. Stefan is currently a Research Fellow in Andrology at CRM since Sept 2014 till Sep 2015. Stefan’s current research is on sperm protein modifications and proteomic profiles in certain reproductive pathologies.

Dr. Eva Tvrdá received her Masters in Biotechnologies in 2009 and a PhD in 2014 from the Slovak University of Agriculture in Nitra, Slovak Republic. Eva received a Fulbright Research Scholar Award to conduct advanced research at the Center for Reproductive Medicine, Cleveland Clinic. She was a Research Fellow in Andrology at CRM from Oct 2013 to Jul 2014. Eva’s novel research at Cleveland Clinic explored the proteins as a possible critical link between testicular cancer and male infertility. Her results (prepared for publication) may explain the cellular and molecular causes of poor semen quality seen in many men diagnosed with testicular cancer prior to the start of their treatment. She hopes that understanding the mechanism of testicular cancer-associated poor semen quality may guide future research in the critical area of fertility preservation.

Dr. Gulfam Ahmad is an Assistant Professor of Physiology, University of Health Sciences, Lahore, Pakistan. He obtained his Master in Clinical Embryology from University of Paris and PhD in Reproductive Physiology from the University of Toulouse, France. Gulfam received a Fulbright Research Scholar Award to conduct advanced research at the Center for Reproductive Medicine, Cleveland Clinic. He is a Research Fellow in Andrology at CRM since Oct 2014 till Oct 2015. Gulfam’s research interests are in the area of male infertility and andrology with a focus on the proteomics of male reproductive cancers. Using cutting edge research techniques, Gulfam aims to identify protein biomarkers and elucidate their role in the pathophysiology of male fertility.

2012

- 20

13

2012

- 20

13

2014

- 20

15

2014

- 20

15

The fact that all the CRM staff were supportive and always ready to help, made the program atmosphere more about taking as much as you could from this experience.

Hanna Tadros, Med Student Jordan Alumni 2014

Our InstitutionCleveland Clinic is ranked as one of America’s Top 4 Hospitals by U.S.News & World Report. Located in Cleveland, Ohio, Cleveland Clinic is a non-profit, multispecialty academic medical center that integrates clinical and hospital care with research and education. Founded in 1921 by four renowned physicians with a vision of providing outstanding patient care based upon the principles of cooperation, compassion and innovation, it is the second largest group practice in the world with 3,200 physicians and scientists practicing and researching in more than 130 medical specialties and more than 1,450 beds. In addition to the main hospital located near Cleveland’s historic University Circle, the Cleveland Clinic operates nine regional hospitals, a children’s hospital, and 17 family health centers in Ohio, as well as hospitals in Florida, Las Vegas, Canada, and Abu Dhabi, employing more than 43,000 individuals and providing global worldclass medical care. Last year alone, 5.5 million patients from over 130 countries and all 50 states received their care at Cleveland Clinic. The main hospital campus, located near downtown Cleveland, occupies 180 acres and 50 buildings. It includes a hospital, an outpatient clinic, a children’s hospital, cancer institute, eye institute, research institute, a medical school, and supporting labs and facilities.

To learn more, visit www.clevelandclinic.org

We Have What You Need To Research Your Way to the Top!

Take a sneak peek into our research laboratories.

TeamworkA large collection of scientific journals, print and electronic journals/books, and other databases are available through the resourceful Cleveland Clinic Alumni Library. Specialized Medical Librarians are ever willing to help in research and use of electronic databases. Statistical research support is readily available from a team of biostatisticians working in the Department of Quantitative Health Sciences (QHS). Services for the design and creation of medical illustration for publication purposes are provided by the experts at The Center for Medical Art and Photography (CMAP) Arts and Photo. These artists also aid in the preparation of research posters and lecture slides for presentations at national meetings.

IT EquipmentAlong with office supplies, the office space for Fellows is equipped with over 2 dozen computers with laser printers, scanners, copiers, and all the necessary software. All computers in the Center are connected to the Cleveland Clinic’s intranet that provides direct access to most online journals hosted by the Cleveland Clinic Alumni Library related to the field of medicine, urology, reproductive medicine, andrology, ob-gyn, etc.

The LaboratoriesResearch and Clinical Laboratories occupy over 5000 square feet of workspace with state-of-the-art instruments and facilities for advanced research in proteomics of male infertility and molecular markers of oxidative stress and DNA integrity.

Core LaboratoriesOur research laboratories has ready access to over a dozen highly-specialized core laboratories within the Cleveland Clinic Lerner Research Institute. These include: proteomics, metabolomics, genomics, molecular biotechnology, molecular screening, flow cytometry, mass spectrometry, translational research, digital imaging and electron microscopy, etc. For more information on these core laboratories, please visit: http://www.lerner.ccf.org/services.

EquipmentInstruments available in our facility include: computer assisted semen analyzers, luminometer for oxidative stress measurements, Accuri BD C6 flow cytometer, equipment for western blotting, culture room equipped with a sterile hood, several incubators, microscopes, and Narishige micromanipulators fitted on an inverted phase-contrast microscope, spectrophotometers, ELISA plate reader, centrifuges, liquid nitrogen storage tanks, programmable freezers, cold room, etc.

Get To Know Our CenterThe Center for Reproductive Medicine is a research program of the Glickman Urological and Kidney Institute of the Cleveland Clinic. Established in 1993, the Center is dedicated to cutting-edge, disease-oriented translational research and to the education of physicians and scientists. Our Center is rated as a leading center of excellence at the world-class Cleveland Clinic and the Department of Urology reigns in its ranking as the No. 1 Urology program in the nation by U.S.News & World Report.

The Center for Reproductive Medicine enjoys an international reputation for excellence and innovation in human reproduction and male infertility research and is dedicated to disseminating its results. Research papers from the Center’s staff are selected regularly for publication in distinguished medical journals. The Center’s website has been ranked No. 1 for research in reproductive medicine and andrology since 2001 by Google and other top search engines, attracting close to 900, 000 visitors to date.

Over the past 21 years, more than 500 physicians, scientists, biologists and medical/pre-med students from over 50 countries worldwide have received their research and ART training at the Center for Reproductive Medicine.

For an up to date list of our publications, kindly visit us on ResearchGate. A Scopus Citation Report of our Research Publications by the Center for Reproductive Medicine from 1993 to 2014 is available here.

Research Fellowship BrochurePublished on: Feb 20, 2015

18 | Cleveland Clinic Center for Reproductive Medicine

Research Fellowship BrochurePublished on: Feb 20, 2015

19 | Cleveland Clinic Center for Reproductive Medicine

Our researchers have: • Published numerous articles in reputed scientific journals, including over 515 peer reviewed original research articles and reviews, and more than 150 book chapters in specialized medical books. To learn more and view our publications, click here.• Presented over 750 research papers at national and international scientific meetings. To read, click here.• Edited over two dozen highly acclaimed medical textbooks or manuals pertaining to human reproduction, male infertility, ART, fertility preservation, sperm chromatin damage and antioxidants. To read more, click here.• Serve as members of editorial boards of esteemed medical journals, such as:

Publications Per Year

Publications with Collaborating Institutions

Areas of Research

Sources of Selected Publications*

Publications with Collaborators

Publications with Collaborating Countries

Types of Publication

Citations Per Year

JOURNALS IMPACT FACTOR NO. OF PUBLICATIONS NO. OF CITATIONS 1 Human Reproduction Update 8.66 3 611 2 Nature Reviews Urology 4.52 3 62 3 Human Reproduction 4.59 10 1087 4 Fertility and Sterility 4.30 90 4316 5 Biology of Reproduction 4.03 2 181 6 Journal of Urology 3.75 16 713 7 Reproductive Biomedicine Online 2.68 26 799 8 Journal of Andrology 3.37 15 889 9 Urology 2.13 29 1028 10 Reproductive Biology and Endocrinology 2.41 17 526 11 Asian Journal of Andrology 2.53 8 207 12 Journal of Assisted Reproduction 1.77 12 131

13 Reproductive Biomedicine Online 2.68 27 981

An Overview ofReproductive Research

1993 - 2014Source: Scopus; http://www.scopus.com

CO

UN

TRIE

S

NU

MB

ER

OF

CIT

ATIO

NS

INST

ITU

TIO

NS

YEARS NUMBER OF PUBLICATIONS

NUMBER OF PUBLICATIONS NUMBER OF PUBLICATIONS

YEARS

NU

MB

ER

OF

AR

TIC

LES*

CO

LLA

BO

RAT

OR

S

http://www.clevelandclinic.org/reproductiveresearchcenter/staff/agarwala.html Last Updated: Sept, 2014

445171

40333332

2418181717

1111109

445

18

17

16

14

13

11

11

10

6

· Ce

n ter

for R

eproductive Medic ine ·

Cleveland Clinic

CENTER FOR REPRODUCTIVE MEDICINE

*Includes original articles and reviews; book chapters are not included

01996-2012 2012 2013 2014

2,000

4,000

8,000

10,000

12,000

6,000

Note: Chapters published in books are not included.

0 10050 150 250 350200 300 400

Agarwal ASharma RK

Falcone TSaid TMGupta S

Sabanegh EEsteves SC

Aziz NHallak J

Paasch Udu Plessis SS

Alvarez JGLoughlin KR

Dada RSikka SC

Other 3.9%

Biochemistry, Genetics and Molecular Biology

Medicine 70.9%

21.0%

Other 0.7%

Conference Paper 1.6%

Book Chapter 2.3%

Review

Original Article65.0%

27.1%

0 10050 200 300 400

United StatesIndia

BrazilEgypt

United KingdomSouth Africa

GermanyCanada

SpainItaly

150 250 350 450

424

37

37

24

19

18

17

15

8

10

0 50 100 200 300 400

Cleveland Clinic Foundation

Androfert Campinas

All India Institute of Medical Sciences

Medical University of SofiaHarvard Medical School

Universidade de Sao Paulo

Tulane University

Universiteit Stellenbosch

Liverpool Women’s Hospital

Universität Leipzig

150 250 350 450 500

01993 1995 1996 1997 1998 2000 2001 2002 2003 2004 2007 2008 2010 2011

20

30

40

10

50

1994 1999 2005 2006 2009 2012 2013 2014

2

7 8

6

19

12

1714 15

12

20

31

36

24 24

3133

45

27

24 25

13

*For a complete list of publications, visit: http://www.clevelandclinic.org/reproductiveresearchcenter/staff/bibagra14.html

Ashok Agarwal, PhD, HCLDProfessor and DirectorCenter for Reproductive Medicine | Cleveland ClinicUnited States of Americahttp://www.clevelandclinic.org/ReproductiveResearchCenter

Tel: +1.216.444.9485Email: agarwaa@ccf.org

Skype ID: ashokagarwal.clevelandLinkedIn ID: Ashok Agarwal

Wikipedia Profile: Ashok Agarwal

RESEARCHGATE http://www.researchgate.net Ashok Agarwal's RG Profile

RG Score 47.74

Total Articles in Publication List: 752 Sum of the Times Cited: 14,053

Average Citations per Article: 18.69h-index: 68

Total Articles in Publication List: 768Books: 26Chapters: 90

Sum of the Times Cited: 14,046Conference papers: 390

Total Impact Points: 1,181.05

WEB OF SCIENCE http://www.wokinfo.com

Note: Only articles from Web of Knowledge with citation data are included in the calculations.Chapters published in the books are not included.

DISTRIBUTION OF CITATIONS BY YEAR

DISTRIBUTION OF CITATIONS BY YEAR

YEARS

WEEKS WEEKS

1,200

2,000

1,000

800

600

400

200

01995 1996 1997 1998 1999 2000 2001 2002 2003 2004 2005 2006 2007 2008 2009 2010 2011 2012 2013

CIT

AT

ION

S

CITATION METRICS

PERCENTILEThis score is higher than 97.5% of ResearchGate members’

0% 50% 100%

58,390Total publication views

78,310Total full-text downloads

PUBLICATION VIEWS

5,188 Total views

PROFILE VIEWS

GOOGLE SCHOLAR http://scholar.google.com

1,400

1,600

1,800

All Since 2008

Sum of the Times Cited: 26,151 17,450

h-index: 86 70

i10-index: 363 309

3688

2766

1844

922

0

19981997 1999 2000 2001 2002 2003 2004 2005 2006 2007 2008 2009 2010 2011 2012 2013 2014

YEARS

CIT

AT

ION

S

006-23 06-30 07-07 07-14 07-21 07-28 08-04

700

1050

350

008-05 08-06 08-07 08-08 08-09 08-10 08-11

21

7

This graph shows the number of times the articles in the publication list were cited in each of the last 20 years.

14

This graph shows the number of times the articles on the publication list have been cited in each of the last 17 years.

· Ce

nter

for R

eproductive Medicine ·

Cleveland Clinic

2014The h-index is an index that attempts to measure both the productivity and impact of the published work of a scientist

Last Updated: September 2014

Ashok Agarwal, PhD, HCLDProfessor and DirectorCenter for Reproductive Medicine | Cleveland ClinicUnited States of Americahttp://www.clevelandclinic.org/ReproductiveResearchCenterBiography - http://en.wikipedia.org/wiki/Ashok_Agarwal

Tel: +1.216.444.9485Email: agarwaa@ccf.org

Skype ID: ashokagarwal.clevelandLinkedIn ID: Ashok Agarwal

Wikipedia Profile: Ashok Agarwal

Ashok Agarwal on Google Scholar

Ashok Agarwal

Ashok Agarwal

Born Lucknow, India

Residence Cleveland, Ohio, USA

Nationality American

Fields Medical Scientist, Researcher,

Andrologist/Reproductive

Biologist

Institutions Cleveland Clinic

Alma mater Banaras Hindu University,

Varanasi, India (M.Sc., Ph.D.),

Harvard Medical School, Boston,

USA (Rockefeller Foundation

Fellowship in Reproductive

Biology)

Known for Research Work in Human

Reproductive Medicine

Website

Center for Reproductive Medicine ,

Tel:+1(216)444-9485

From Wikipedia, the free encyclopedia

Ashok Agarwal is the Director of the

Andrology Center, and also the

Director of Research at the Center for

Reproductive Medicine at Cleveland

Clinic, Cleveland, USA.[1] He is

Professor at the Cleveland Clinic

Lerner College of Medicine of Case

Western Reserve University, USA.

Ashok is a Senior Staff in the

Cleveland Clinic's Glickman Urological

and Kidney Institute.[1] He is well-

recognized around the world for

prolific and cutting-edge translational

research in human infertility and

assisted reproduction.

Contents [hide]

1 Education

2 Professional Life

3 Publications, Editorship and

Reviewership

4 Research Interests

5 Recent Awards

6 Authorship

6.1 Scientific Publications

6.2 Books

7 References

8 External links

Education [edit]

Ashok obtained his B.Sc. (Honors) in 1975, M.Sc. in 1977 and Ph.D. in 1983 at

Banaras Hindu University, Varanasi (India).[2] He did his post-doctoral research on

a Rockefeller Foundation Fellowship at Harvard Medical School and the Brigham

and Women's Hospital[2] in Boston, Massachusetts (1984-1986).

Article Talk Read Edit View historyMore Search

Edit links

Main page

Contents

Featured content

Current events

Random article

Donate to Wikipedia

Wikimedia Shop

Interaction

Help

About Wikipedia

Community portal

Recent changes

Contact page

Tools

What links here

Related changes

Upload file

Special pages

Permanent link

Page information

Wikidata item

Cite this page

Print/export

Create a book

Download as PDF

Printable version

Languages

Create account Log in GLOBAL RANKING OF AUTHORS PUBLISHING INANDROLOGY OR MALE INFERTILITY

Source: SCOPUS database (www.scopus.com)Keywords: Andrology or Male Infertility

Documents scanned: 73,647Search Field: All Fields

Subject Areas: Life Sciences and Health SciencesDocument type: All

Years published: All Years to Present

Number of Publications Citations h-index

Top 10 Authors

Agarwal, A Nieschlag, E Diedrich, K Devroey, P Aitken, R.J. Tournaye, H Schill, W.B. Van Steirteghem, A. Check, J.H. Schlegel, P.N.

01 2 3 4 5 6 7 8 9 10

50

100

150

200

250

300

350315

264

201 196 182 178 172161 157 151

Original Articles & Reviews

Agar

wal

Nies

chla

g

Died

rich

Devr

oey

Aitk

en

Tour

naye

Schi

ll

Van

Stei

rtegh

em

Chec

k

Schl

egel

0

1000

2000

3000

4000

50004565

3087

2241 22392028

1423 1301

712 532259

1 2 3 4 5 6 7 8 9 100

30

20

10

40

50

60

7064

54

4642

61

26

61

19

58

29

1 2 3 4 5 6 7 8 9 10

(search date: Dec 15, 2013)

Documents scanned: 53,900Document type: Original Articles

Documents scanned: 11,382Document type: Reviews

Num

ber

of P

ublic

atio

ns

Num

ber

of P

ublic

atio

ns

Cita

tions

Cita

tions

h-in

dex

h-in

dex

GLOBAL RANKING OF AUTHORS PUBLISHING INANDROLOGY OR MALE INFERTILITY continued

Source: SCOPUS database (www.scopus.com)Keywords: Andrology or Male Infertility

Search Field: All FieldsSubject Areas: Life Sciences and Health Sciences

Document type: AllYears published: All Years to Present

Top 10 Authors Top 10 Authors

Agarwal, A Nieschlag, E Diedrich, K Devroey, P Aitken, R.J. Tournaye, H Schill, W.B. Van Steirteghem, A. Check, J.H. Schlegel, P.N.

Notes:1. The search results portrayed in this document were generated on Dec 15, 2013 and have been independently verified by a Librarian as well as 3 different researchers.2. These search results are reproducible using Scopus and by employing the exact same key words and search criteria as outlined at the top of this page. However, these results are subject to change following daily updates of the Scopus database.3. The total number of articles (or search outcome) generated during a Scopus search is dependent on the specific search term(s) or key word(s) used as the search criteria. The publication numbers used in this document accurately tallies the number of articles retrieved using the specific search query of “Male Infertility or Andrology”. It does not, however, represent the total number of publication by an individual author using other search terms. For example, when searching the Scopus database on Jan 3, 2014, the total number of publications for Agarwal, A. was 327 (using the search query of “Male Infertility or Andrology”) and 449 (using the search query of “Author Name and Affiliation”). 4. The results generated using Web of Science was quite similar to Scopus, by employing the same search techniques as used in this report.5. The content coverage in the Scopus database for abstracts go back to 1966 and for cited references go back to 1996.6. The h-index attempts to measure both the productivity and impact of the published work of a scientist or scholar. The index is based on the set of the scientist’s most cited papers and the number of citations that they have received in other (scientists’) publications. For details refer to: http://en.wikipedia.org/wiki/H-index

Last updated: Jan 3, 20132

0

500

1000

1500

2000

25002453

1725

1160

216

18201757

1126

3087

438 429

1 2 3 4 5 6 7 8 9 10

01 2 3 4 5 6 7 8 9 10

50

100

150

200

250220

186

162147

127 125 123113 107 101

Original Articles

Agar

wal

Nies

chla

g

Died

rich

Devr

oey

Aitk

en

Tour

naye

Schi

ll

Van

Stei

rtegh

em

Chec

k

Schl

egel

0

20

10

30

40

50

60

4643 42

40

53

48

58

25 24

19

1 2 3 4 5 6 7 8 9 10

0

20

40

60

80

100

83

46 45 45

27 25 2521 20 17

1 2 3 4 5 6 7 8 9 10

Review Articles

0

500

1000

1500

20001942

349264

193 15866 38

172

1069

424

1 2 3 4 5 6 7 8 9 10

0

10

5

15

20

25

35

30

1513

12 12

5

23

18

33

10

5

1 2 3 4 5 6 7 8 9 10

• Advances in Bioscience and Clinical Medicine• American Journal of Molecular and Cellular Biology• Andrology• Asian Journal of Andrology• Fertility and Sterility• Human Andrology• Indian Journal of Experimental Biology• Human Fertility• International Brazilian Journal of UrologyD

id Y

ou K

now

?

• International Journal of Biotechnology and Bio Sciences• Journal of Clinical Embryology• Journal of Reproductive and Stem Cell Biotechnology• National Journal of Andrology• Translational Andrology and Urology• Open Reproductive Science Journal• Reproductive BioMedicine Online• Reproductive Biology & Endocrinology• World Journal of Men’s Health

Dolores Lamb, PhD Sijo Parekattil, MD Ashok Agarwal, PhD andBotros Rizk, MD, PhD

Peter Nagy, MD, PhD, Kim Ly, MBA,Dolores Lamb, PhD, Ashok Agarwal, PhD

Anthony J Thomas, MD andRachel Thomas, BSc

Rakesh Sharma, PhD andDoris Baker, PhD

Rima Dada, MD, PhD

agarwaa@ccf.org+1.216.444.4985

Ashok Agarwal is the Director of Research at the Center for Reproductive Medicine and the Director of the Andrology Center. He holds these positions at Cleveland Clinic, where he is a Professor at the Lerner College of Medicine of Case Western Reserve University and, since 1993, Senior Staff in the Glickman Urological and Kidney Institute. Dr. Agarwal did his post-doctorate training in Reproductive Biology under a fellowship from The Rockefeller Foundation at Harvard Medical School in Boston, Massachusetts. He was an Instructor in Surgery and then an Assistant Professor of Urology at Harvard Medical School from 1988 to 1992. Dr. Agarwal was appointed in 1993 by the Cleveland Clinic Foundation, Ohio, as the Head of the Clinical Andrology Center, which over the years under his leadership, has become a center of excellence for the diagnosis of male infertility and fertility preservation of men with oncological conditions in the United States. He is board certified as a Clinical Laboratory Director in Andrology by the American Board of Bioanalysis and serves as an Inspector for the College of American Pathologists “Reproductive Laboratory Program” for accreditation of Andrology & IVF Laboratories.

Dr. Agarwal’s h-index is 90 (Google Scholar) and 68 (Web of Science and Scopus) respectively, while his citation count is nearly 30,000 on Google Scholar as of Feb’ 2015. His RG score is 50.35 on 1,384 publications (797 articles, 26 books, 92 chapters and 395 conference papers). Dr. Agarwal is ranked as the #1 Author in Andrology/Male Infertility and ART-related research, based on a Global Ranking of Authors in Andrology or Male Infertility report employing exhaustive searches of the Scopus database. Dr. Agarwal received the 2011 and 2012 Innovator

Award for the development of “Remote Sperm Banking Kits” from the Cleveland Clinic Innovations. He was thrice-awarded the Scholarship in Teaching Award in 2011, 2013 and 2014 by the Case Western Reserve University Medical School in recognition of his innovative Summer Internship Course in Reproductive Medicine, which has been held annually since 2008. Dr. Agarwal was recognized by a second Scholarship in Teaching Award in 2013 and 2014 for his distinctive Hands-On Training Program in Advanced Reproductive Techniques, which has been conducted yearly since 2003. Dr. Agarwal’s current research interests are identifying biological markers of oxidative stress, DNA damage and apoptosis using proteomic research tools and bioinformatics analysis as well as preserving fertility in patients with cancer. He is actively involved in laboratory and clinical studies assessing the efficacy of certain antioxidants in improving the fertility of male patients.

Read more about Dr. Agarwal on LinkedIn (link to http://www.linkedin.com/pub/ashok-agarwal/57/819/46), Research Gate (http://www.researchgate.net/profile/Ashok_Agarwal2/), and Wikipedia (https://en.wikipedia.org/wiki/Ashok_Agarwal).

Ashok Agarwal, PhD, HCLD (ABB), EMB (ACE) | Professor and Director

The bar chart above depicts yearly citations from 2007 to 2015 only. Search date: Feb 02, 2015

To assess the most up to date citation indices, search “Ashok Agarwal, PhD, HCLD” on Google Scholar or visit

https://scholar.google.com.my/citations?user=FP_jRpIAAAAJ&hl=en&oi=ao

* ‘All’ refers to yearly citation indices from 1998 to 2015

Meet the Director

Search results from Feb’ 2015

· Ce

nter

for R

eproductive Medicine ·

Cleveland Clinic

Reproductive Research Alumni1993 - 2014

Australia

Austria

Bahrain

Brazil

Bulgaria

Canada

Chile

China

Colombia

Croatia

CzechRepublic

Egypt

Greece

Guyana

Hong Kong

India

Iran

Iraq

Italy

Jamaica

Japan

Jordan

Kazakhstan

Korea

Kosovo

Kuwait

Lebanon

Malaysia

Mexico

Nigeria

Phillipines

Poland

Romania

Russia

Saudi Arabia

SlovakRepublic

South Africa

Spain

Syria

Turkey

UAE

Venezuela

Research Fellowship BrochurePublished on: Feb 20, 2015

20| Cleveland Clinic Center for Reproductive Medicine

21 | Cleveland Clinic Center for Reproductive Medicine Research Fellowship BrochurePublished on: Feb 20, 2015

· Ce

nter

for R

eproductive Medicine ·

Cleveland Clinic

Reproductive Research Alumni1993 - 2014

22 | Cleveland Clinic Center for Reproductive Medicine Research Fellowship BrochurePublished on: Feb 20, 2015

· Ce

nter

for R

eproductive Medicine ·

Cleveland Clinic

Reproductive Research Alumni1993 - 2014

Glickman Urological Institute TowerReflecting pools in front of Miller Pavillion

(Heart Institute)

Research FellowshipUpdated on 12.2.2011

For more information about Cleveland, visit these resources:About Cleveland, Cleveland.com, Cleveland, The New American City, Positively Cleveland, Travel Cleveland, and Discover Ohio.

23 | Cleveland Clinic Center for Reproductive Medicine

Cleveland is the second largest city in the state of Ohio, after Cincinnati. Located on the southern shores of Lake Erie, Cleveland lies at the outflow of the Cuyahoga River into Lake Erie. The main campus of Cleveland Clinic is situated 5 miles east of downtown Cleveland. Case Western Reserve University and University Hospitals are within one mile of Cleveland Clinic. Cleveland State University and John Carroll University are also within a short distance of the Cleveland Clinic main campus. Nearby the Cleveland Clinic is University Circle, which serves as the cultural, medical and education center of Cleveland and Northeast Ohio. Medical research in the University Circle institutions places Cleveland as one of the major medical research environments in the country.

University Circle is also the home to the Cleveland Museum of Art, the Cleveland Institute of Music, the Cleveland Institute of Art, and the Museum of Natural History. The Cleveland Museum of Art houses one of the country’s most highly acclaimed art collections. The world-famous Cleveland Orchestra, performs regularly in the elegant Severance Hall. Playhouse Square is the home to several large theaters that host traveling Broadway plays and various other performances.

The University Circle area is walking distance from Cleveland’s historic Little Italy with its brick streets, a quaint and charming area of intimate Italian

Art Museum Duck Pond in Spring Cleveland skyline in Summer Autumn in the Park Lake Erie in Winter

Cleveland — a great place to call HOME!

Rock and Roll Hall of Fame

State Theater Playhouse Square Beachwood Place Mall

Cuyahoga Valley National Park Edgewater Park

Browns Stadium

restaurants, antique and craft shops, and art galleries. The Coventry Village area is close to the Clinic’s main campus and popular with its shops and restaurants.

Cleveland hosts the Rock and Roll Hall of Fame and Museum, and the Great Lakes Science Center. Outdoor enthusiasts of all ages enjoy the ‘Emerald Necklace’, the Cleveland Metroparks network of parks that encircles the city, which offer a variety of recreational opportunities. When it comes to professional sports, Cleveland is the proud home to the Browns football, Cavaliers basketball, Indians baseball and Lake Erie Monsters hockey teams respectively. As the Great Lakes city on America’s North Coast, Cleveland offers abundant lakeshore for different activities.

Cleveland and its surrounding suburbs are home to nearly 3 million residents. A vibrant and versatile metropolitan area, Cleveland has nearly 80 residential communities. Fine residential areas are located within minutes of the Cleveland Clinic campus. Recognized as one of the best places to live and visit, Cleveland and North-East Ohio is bustling with exciting things to do for people of all ages.

CopyrightCenter for Reproductive Medicine, Cleveland Clinic Foundation, United States of America

Editor: Ashok Agarwal l Consultant: Damayanthi Durairajanayagam l Graphic Design: Bernastine BuchananFirst Edition: Feb 20, 2015