ADVIA Centaur Immunoassay System BYZEESHAN YOUSUF

Technology

• Chemiluminescence is a chemical reaction that emits energy in the form of light. When used in combination with immunoassay technology, the light produced by the reaction indicates the amount of analyte in a sample. Direct chemiluminescent reactions directly measure the light energy without the use of added steps or amplifying molecules. The ADVIA Centaur XP assays use acridinium ester (AE) as the chemiluminescent label, because AE does not require the addition of a catalyst or substrate.

• Direct ChemiluminescenceIt is easy to automate direct chemiluminescence using AE

and provides many benefits, such as long reagent shelf life, fast reaction time, and assay sensitivity. The ADVIA Centaur XP assays use the dimethyl form of AE because its stability allows long reagent shelf life.

Assay Reaction Formats

The system uses a variety of formats to detect antigens as well as antibodies.

1

sandwich format

2

competitive format

3

antibody-capture format

ADVIA CENTAUR COMPONENTS

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SYSTEM PROCESSING AREAS

Comprehensive Menu• 30 on board reagents maximize test capacity

Powerful Productivity• 240 tests per hour• High-resolution touch screen for easy interaction• Expanded ancillary reagent and fluid capacity• Universal sample rack design eliminates manual

tasks

• STAT port guarantees priority sampling at any time

Always Ready Replenish Supplies without Pausing 840 tips

USE OF SYSTEM

TECHNICAL SPECIFICATION

SAMPLE HANDLING

Sample Tubes•1 mL, 2 mL, 3 mL, 5 mL, 7 mL, 10 mL, micro container tubes

Automatic Dilutions •Varies by assay, up to 1:1000

Sample Handling •Serum, plasma, urine - assay dependent

Sample Volume •10 uL to 200 uL assay dependent

Sample Integrity Control •Pressure transducer technology with clot management, detection, and flagging.

REAGENT HANDLING

Reagent Cooling •30-position reagent tray cooled 4°C to 8°C

Reagent Integrity Control •Barcode reagent identification, automatic inventory tracking and flagging, calibration validity tracked and flagged, reagent on board stability tracked and flagged, reagent expired/ reagent low flagging

Reagent Mixing •ReadyPacks automatically rocked onboard

Quality Control

• Advanced QC package includes Levy-Jennings plots and Westgard rules; 65,000 control results can be stored• Results within ± 2SD accepted

TYPES OF ASSAYS

Allergy •Total IgE

Anemia•Ferritin•Folate•RBC Folate•Vitamin B12

Bone •Vitamin D

Tumor Markers/Oncology

•AFP•BR•CA 15-3•CA 19-9•CA 125II•CEA•Complexed PSA•Free PSA•PSA•Serum HER-2/neu

ToRCH and Special ID

•CMV IgG•CMV IgM•Rubella IgG•Rubella IgM•Syphilis•Toxo IgG•Toxo IgM

Cardiovascular

•BNP•CKMB II•D-Dimer•Myoglobin•Tnl-Ultra

Diabetes•C-Peptide (serum, urine)•Insulin

Hepatitis and

HIV

•Anti-HBe•Anti-HBs•Anti-HBs-2•eHIV 1/O/2†•HAV IgM•HAV Total•HBc Total•HBc IgM•HBe Ag•HBs Ag•HBs Ag Confirmatory•HBs AgII•HCV†•HIV Combo (CHIV)†

TDM

•Carbamazepine•Digitoxin•Digoxin•Gentamicin•Phenobarbital•Phenytoin•Theophylline•Tobramycin•Valproic Acid•Vancomycin

Liver Fibrosis

•HA (ELF Marker)•TIMP (ELF Marker)•PIIINP (ELF Marker)

Metabolic •Cortisol (serum, urine)•Homocysteine

Reproductive Endocrinolog

y

•AFP•DHEAS•Estradiol-6•Estradiol-6 III•FSH•LH•Progesterone•Prolactin•SHBG•Testosterone•Total hCG

Immunosuppresants

•Cyclosporine•Tacrolimus*

Sepsis •Procalcitonin

Thyroid Function

•Anti-TG Ab•Anti-TPO Ab•Free T3•Free T4•Intact PTH•TSH•Third Generation TSH•T Uptake•Total T3•Total T4•TSH 3-UL