Ivf instruments overview

INFERTILITY OVERVIEW

Infertility means difficulty in conceiving (becoming pregnant) despite having regular sex without using contraception. There is no definite cut off point to say when a couple is “infertile’ many couples take several months to conceive. About 17 in 20 couples conceive within a year of trying. About 19 in 20 couples conceive within two years.

Looking at this another way, about 1 in 7 couples do not conceive within a year of trying. However, more than half of these couples will conceive over the next year without any treatment.

IUI (INTRA UTERINE INSEMINATION)

• An intrauterine insemination (IUI) is a procedure in which the sperm is • “washed” and prepared then deposited into the upper portion of the uterus to

help achieve a pregnancy. It is often in excellent option for couples prior to considering more complicated and very expensive procedures, such as in vitro fertilization(IVF).

• The wash and IUI can be very helpful to couples seeking to have a child when the man has low sperm counts, low motility, hyper viscosity (very thick semen), motile sperm that are clumped together (antibodies), sperm that “ backs up” into the bladder (retrograde ejaculation) or other abnormal sperm test such a poor sperm shape (morphology). Many couples use IUI to accelerate the chances of pregnancy, such as after vasectomy reversal or if the woman is older.

• It is also useful when the woman’s mucus is hostile or compatible with man’s sperm, or if she has problems with her cervical positioning or opening, as well as unexplained causes of infertility.


Methods of Sperm Preparation.1. The fundamental objective of the various preparation procedure employed is

to separate the spermatozoa from the seminal plasma as thoroughly as possible with minimum mechanical force. These are adequate for normal semen sample. However, as the quality of reducing the level of physical damage inflicted upon the spermatozoa and at the separating the contaminating material. The modifications fall into the following basic categories.

The basic aim of semen washing / processing is as follows:1. Removal of seminal plasma.2. Removal of non motile sperms, morphologically defective and immature

sperms.3. Removal of debris and pus cells.4. Concentration of motile sperms.5. Capacitating of motile sperms.

EQUIPMENTS REQUIRED FOR AN ANDROLOGY LAB 1. LAMINAR AIR FLOW LAVA ANDROLOGY WORKSTATION LAVA workstation offers possibility to control temperature & cleanliness. Fully made of MS with powder coat finish Pre-filter, Minipleat(separator less) HEPA filters Imported blowers with noise level of less than 55dBA. Inbuilt Incubator for semen storage Image processing system along with software for IUI


EQUIPMENTS REQUIRED FOR AN ANDROLOGY LAB. 2. TRINOCULAR MICROSCOPE LABOMED LX 300

Adapted for all types of lab environments, the new Lx 300 reflects a fresh design and delivers a rugged exterior with quality optical and mechanical ingredients for a lifetime of carefree use. This next generation microscope asserts its sophistication not only by design, but also through flawless functionality. In light of the latest in manufacturing technology, the highly modular Lx 300 can offer you a high degree of performance for various applications. Our LP series objectives enable you to view an assortment of specimens with the highest degree of clarity. Lx 300’s Plan Achromatic optical system has been With a compact design and robust mechanical stage with fluid X/Y operation, maintenance free is a standard. Lx 300 offers you flexibility in illumination. You can stay with the standard 20W high efficiency halogen bulb, or you can opt for a Bright LED, providing illumination in a near daylight spectrum, with Battery Back.


3. SPERM COUNTING CHAMBER The Makler Counting Chamber is a simple to use device for Sperm Count and motility evaluation from undiluted semen. Depth of only 10 microns. Constructed with two pieces of optically flat glass, cover glass upper, 2nd layer with a 1sq.mm finde grid in the centre subdivided into 100 squares of 0.1x0.1 mm each. Percentage and quality of motility and sperm counts are easily obtained.


4. CENTRIFUGE R-4C Compact model is designed for routine work in

medical and industrial laboratories. It has digital speed indicator and 0-60 minute digital countdown timer.

R-4C DX has additional features of dynamic brake for quick deceleration & imbalance detector with cutoff in case of uneven loads.

Salient Features: Stepless speed regulator Safety Lid interlock to prevent cover opening during centrifugation Dynamic brake for quick deceleration (R-4C DX) Imbalance detector with cutoff (R-4C DX)


5. HEATING BLOCK

Fully made out of SS 304 – dully polished. OMRON German controller for better accuracy. 3 no’s of anodised aluminium blocks interchangeable with workstation blocks. Each block can hold 6 no’s of 14ml falcon tubes.


6. IUI MEDIAS – GYNAMED1. GM 501 SPERM ACTIVE – SWIM UP 2. GM 501 GRADIENT - DOUBLE DENSITY GRADIENT

1. SPERM PROCESSING MEDIA GM501 SPERM ACTIVE GM501 Sperm Active is a ready-to-use medium designed for all sperm-preparation, sperm-washing and swim-up techniques like density-gradient centrifugation as well as for testicular tissue.

2. SPERM PROCESSING MEDIA GM501 GRADIENT GM501 Gradient is a saline-silica based density gradient medium used in ART for separation and purification of highly motile human Spermatozoa. A dual density Gradient system (45% and 90%) starting from GM501 Gradient 100% If preferred, a multi-layer may be used as well (e.g. 45%-70 %-90 %).


7. SAFE 3.1 - Software Assisted Fertility carE 3.1

Andrology software for Semen Analysis, Processing (pre wash / post wash) & report.


IVF (IN VITRO FERTILISATION)

In vitro fertilisation (IVF) is a process by which an egg is fertilised by sperm outside the body: in vitro. IVF is a major treatment for infertility when other methods of assisted reproductive technology have failed. The process involves monitoring a woman's ovulatory process, removing ovum or ova (egg or eggs) from the woman's ovaries and letting sperm fertilise them in a fluid medium in a laboratory. When a woman's natural cycle is monitored to collect a naturally selected ovum (egg) for fertilisation, it is known as natural cycle IVF. The fertilised egg (zygote) is then transferred to the patient's uterus with the intention of establishing a successful pregnancy. The first successful birth of a "test tube baby", Louise Brown, occurred in 1978. Louise Brown was born as a result of natural cycle IVF. Robert G. Edwards, the physiologist who developed the treatment, was awarded the Nobel Prize in Physiology or Medicine in 2010.

ICSI (INTRA CYTOPLASMIC SPERM INJECTION)

• ICSI is generally performed in addition an in vitro fertilization procedure to extract often several oocytes from a woman.

• The procedure is done under a microscope using multiple micromanipulation devices (micromanipulator, microinjectors and micropipettes). A holding pipette stabilizes the mature oocyte with gentle suction applied by a microinjector. From the opposite side a thin, hollow glass micropipette is used to collect a single sperm, having immobilised it by cutting its tail with the point of the micropipette. The oocyte is pierced through the oolemma and into the inner part of the oocyte (cytoplasm). The sperm is then released into the oocyte.

IVF/ICSI - PROCEDURE

• CONTROLLED OVARIAN HYPERSTIMULATION FOR MULTIPLE FOLLICLE DEVELOPMENT.

• MONITORING OF THE TREATMENT CYCLE.• OOCYTE RETRIVEAL.• FERTILIZATION INVITRO.• EMBRYONIC DEVELOPMENT IN VITRO A. LASER ASSISTED HATCHING B. CRYO PRESERVATION• EMBRYO TRANSFER• LUTEAL SUPPORT• FOLLOW UP

STAR IVF WORK STATION Fully made of MS with powder coat finish. Pre-filter, Minipleat (separator less) HEPA filters. Imported blowers with noise level of less than 55dBA. State-of-art Heating System for Table and Stage Heating Block Integrated Customize Heating Areas using the standard available heat patterns. Integrated Stage Warmer with stereo zoom mounting according to your microscope model.



OOCYTE COLLECTION - ASPIRATION PUMP

Digital display of set & actual vacuum. Digital Low volume Vacuum pump. Uniform flow pattern. Max Vacuum attained 400mm of Hg. With overflow bottling & battery backup. Wired & wireless foot pedal.


STEREO ZOOM MICROSCOPE OLYMPUS SZX 10

The SZX10 is designed for routine research. It offers a high numerical aperture (NA) of 0.2, producing a resolution of 600 line pairs per millimeter. With this resolution you can work more efficient, more precise and gain much more information from your samples. The SZX10 provides a zoom ratio of 10:1. With the common objective design it is a fully modular system offering a range of options.


CO2 INCUBATOR – MEMMERT - IN 108 (BOX TYPE) Certified Medical device. Sterilization Temp.:160° C for 4 hrs. IR CO2 Sensor. No need to remove any sensors during Sterilization. 2 Pt100 sensors Class A in 4-wire-circuit,mutually monitoring

and taking over the performance at the same temperature value. Electro polished interior seamlessly welded by laser. Integrated humidity limit control (88-97%) with digital display of relative humidity – resolution of display : 0.5%, setting accuracy:1%. Adaptive, fuzzy-supported, multifunctional, digital microprocessor

PID-controller. Digital display (LED) of all set parameters, such as temperature, weekdays, time, CO2, humidity

and set-up values - language to be chosen in setup. Independently working, digitally adjustable electronic over temperature controller TWW

protection class 3.1. intrinsic adjustment of altitude above sea level.


TRI GAS INCUBATOR – K-SYSTEMS - G185 (BENTCH TOP) 6 separate sensors to control and regulate temperature . CO2 & O2 gas environment can be adjusted. CO2 Range : 2-10% : O2 Range : 2-20%. Grade A air quality. Circulating through UV light chamber; HEPA/VOC filter. Fast recovery : CO2 < 2min : O2 < 4min. 10 chambers each accompanying 4 dishes. Individual gas flow for each chamber. Easy cleaning – warming blocks can be autoclaved. Minimised risk of cross contamination, 10 micro environment chambers.


CARL ZEISS WITH S MICROMANIPULATOR Carl Zeiss Microscopy GmbH – Germany inverted microscope Axio vert.A1. 13mm focus lift with adjustable focus stop. Binocular phototube, 45°/23 (50:50) Green Filter, d=32x2mm. Neutral density filter 0.06. Optical equipment phase contrast and plasDIC. Objective LD A-plan 5x/0.12 ph 1 M27, 10x/0.25 ph 1 M27 & 20x/0.35 ph 1 M27. LD Condenser 0.4 for slider, for H, Ph, plasDIC, VAREL for objectives 5x – 63x 53mm working distance. Camera Adapter 60N-C 2/3” 0.5X. S-micromanipulator: MO-20 3 Dimensional with joy-stick (Micromanipulator) Universal Joint, X/Y/Z 20mm each, 2mm one revolution handle, joy-stick on X/Y plane 2mm/max. IA-1 Pneumatic injector. Injection holder set, 15mm.


ICSI MANIPULATOR - EPPENDORF


OKO LAB – T GLASS Transparent and flat surface at controlled temperature. Built in temperature sensor with 3.2” touch screen PID controller. External temperature sensor for self calibration routine to obtain media temperature on petri plate. Temperature range : from room to 40°C Temperature accuracy on specimen : + 0.3°C Glass thickness : 0.5 mm

System features:PLATE : #1 round heated plate with built in temperature

sensor.CONTROLLER : Multichannel temperature controller. with 3.2” touch screen and Self Calibration Routine. #1 External temperature sensor.RS232 for Data Logging (Optional)Data Logger (Optional)

SAFE - ET - Software Assisted Fertility carE 3.0

Embryo Transfer software.



VOC METER Real time monitor ambiance air quality. Semiconductor mix gases sensor with 5 year life. Gas detection : cigarette smoke, VOC’s such as formaldehyde and Toluene, ethanol, ammonia, hydrogen sulfide, sulfur dioxide and Other harmful gases. Monitor temperature and relative humidity. Three-color (Green/Yellow/Red) LCD backlit indicating air quality At optimal/moderate/poor. Provide 1x on/off output to control a ventilator. High quality technics, elegant appearance & CE-approval.


DYLOS AIR QUALITY MONITOR – DC1100 A true Laser Particle Counter with 2 size ranges 1. small (bacteria, mold, etc.) 2. large (pollen, etc.) DC1100 features technology and engineering that allows monitoring of indoor air quality with an LCD screen that provides small and large particle counts with a dynamic bar graph showing actual count reading. Multiple modes including minute, hour, day and monitor to evaluate your air quality. Store up to 30 days of air quality history for review.


DYLOS AIR QUALITY MONITOR Specially designed for the continuous monitoring of CO2 incubators. Improved accuracy on CO2 readings. Quick verification of CO2 incubator levels. Time saving with dual temperature probes option. Large data storage and user friendly software and download. Easy to read large well lit display. CO2 measuring range 0-20%. Optional : Temperature probe for incubator Oxygen module Humidity module


DIGITAL THERMOMETER

0.1 ° C resolution over the full range. Max/min, hold & auto power off facility. Range of -20 to + 50°C. Resolution of 0.1 °C of 0.1 °F. Thermometers never need re-calibration.


CRYO PRESERVATION UNIT Embryo cryopreservation is useful for leftover embryos after a cycle of in vitro fertilisation, as patients who fail to conceive may become pregnant using such embryos without having to go through a full IVF cycle. Or, if pregnancy occurred, they could return later for another pregnancy. Spare oocytes or embryos resulting from fertility treatments may be used for oocyte donation or embryo donation to another woman or couple, and embryos may be created, frozen and stored specifically for transfer and donation by using donor eggs and sperm.

REPRODUCTIVE TECHNOLOGIES INDIA PVT LTD.,10 BALAMUTHU STREET, ELLIS ROAD,MOUNT ROAD,CHENNAI – 600 002.

www.reproductivetechnologies.inE.MAIL : [email protected] : 044 - 43567378

Health & Medicine

Ivf instruments overview