Gear Grants

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SC Statewide Alliance for Tissue Biofabrication and Gear Grants support by Roger Markwald, PhD

Text of Gear Grants

GEAR GRANTS

GEAR GRANTS & RELATIONSHIP TO CURRENT NSF RII GRANT

GEAR GRANTSDue March 29 2010 Pilot projects targeted to submitting an extramural grant within 12 monthsAmount of awards (50-100K)Peer Reviewed 10 pages max (interdisciplinary)ALIGN with NSF RII Track 1 Award

GEAR GRANTSInstructions (SC EPSCOR OFFICE)

Scott Little (Little@scra.org)Isabel Sanchez (SANCHEZ@scra.org)

Key: alignment with the NSF RII grant The South Carolina Project

NSF-RII Grant

Title South Carolina Project Theme Tissue Based EngineeringGoal To Engineer a Vascular Tree by Bioprinting

What is Bioprinting?Bioprinting/Biofabrication is a computer-aided, robotic layer by layer addition of living building blocks to form 3D functional human tissue constructs

BIOPRINTINGthe ability to arrange bio-materials in a controlled but additive fashion in three-dimensional (3D) space.

BioPrinting Using BioInkBioInk = aggregates of stem cells + hydrogel (morphogens)

ABCDEFGFrom Mironov, Markwald et al 2009 in Expert Opinion in Biological Therapy (in press)BioInkBioInkStem cells + hydrogel

SOUTH CAROLINA PROJECT

Digital AnatomyBlueprintCADBioinkBioprinterBiopaperMATUROGENSTissue ConstructsBiomonitoringI. Pre-processingII. ProcessingIII. Post-processingComponents ofOrgan PrintingTechnologyBioreactors

Enabling Technologies Required for Engineering a Vascular Tree

*Grants for exploratory academic research**Key to engineering vasc tree is bioprinting - building blocks, must be living tissue and without mortar to hold them in place*(bio in a broad sense meaning synthetic biocompatible materials, natural extra-cellular matrices, cells, genes and proteins/peptides)*Also experience in developmental biology to know how to direct building blocks into specific tissue types - fuse seamlessly- began with laser jet printers in which print cartidges were filled with cells. *Our bioink comes in different colors representing diff. stem populations - building blocks are spheroids of 100K cells (breakthough) can be dispensed by robotic printers unlike brick which requires mortar, we were able to show several years ago that living building blocks or spheres will fuse seamlessly within a layer and between layers - come in different colors so to speak in printing an organ you need function tissue that carries out fc like absorption, excretion, contraction (point our gold blocks) but the functional tissue needs oxygen which means blood supply our plan thus is to first engineer the green tissue, the vasculare tissue , then fill in with functional gold blocks - thus the SC project is to print engineer the vascu tissue *vHow it will be pursued,, envision biofabrication or bp as requiring 3 major steps pre proc post called them thrusts note that computer cyberstructure important in first step, must start with blueprint that is computer designed / bioink is stem cells biopaper is hydrogel or biomaterials added to aggregrates that contain cues for what to become, lineage signals, biopaper could also be scaffold needed for larger bore vessels -- most challenging steps will be postprocessing bioreactor will be key for maturation as it will allow for shear stress, flow/hemodynamics to act on printed structure, can accelerate maturation in a bioreactor but to accomplish rapid prototyping, have to find a way to reduce what takes nature weeks or more to just days for this much of the scientific thrust of the project will be to isolate and introduce maturogens what are these?? Molecules nature delivers slowly over time to promote maturation, we have isolated some of them and will deliver in larger amounts, more quickly*ATBC IS THE STRATEGIC HUB FOR IMPLEMENTING THE STRATEGIC THEME ENTER ---- based at MUSCATBC WILL BE A PHYSICAL PLACE WHERE TECHNOLOGIES NEEDED TO ENGINEER VASCULAR TREE CAN BE PERFORMED OR COORDINATED NEEDED TO PERFORM THRUSTS WILL HOUSED IN ATBC /// KEY NEW BIOE BLDG = RECRUIT NEW TALENT //ANOTHER KEY WILL BE SUSTAINABILITY REASONS NSF MADE SUCH A LARGE INVESTMENT IS THAT IT HAS REAL POTENTIAL TO EXPAND WORKFORCE BROADEN PARTICIPATION TO INCLUDE EVERYONE ALL THIS AND MORE BUT ALSO BEC IT IS AN ETHICHAL AND SCIENTIFICALLY SOUND SOLUTION TO SOLVING WHAT HAPPENS WHEN ORGANS FAIL AND THERE IS ONLY LIMITED SUPPLY OF DONOR TISSUES FOR TRANSPLANT NOT ONLY WILL SCIENCE BE COORDINATED BY ATBC BUT ALSO OUTREACH ACTIVITIES INCLUDING EDUCATION, DIVERSITY AND INDUSTRIAL

THUS ATBC IS THE NODAL PLAYER IN STRATEGIC PLAN

NEXT SLIDE SHOWS THE FLOOR PLAN FOR ATBC