By Stephanie Wilson

The Creation of Transgenic Mice

How do you create a Transgenic Mouse?

•Several methods but one most often used:

Transgenic Mouse Process Animation

First Method of Transgenic Mice Creation

• (basic steps) Standard Transgenic Approach:1. Microinject fertilized mouse eggs with

transgene

2. Injected eggs are implanted into a foster mother

Methods continued…

Methods continued…

3. Offspring are then born “Pups”; possible founders (have the transgene)

Method Continued…

4. Potential founders (pups with the transgene) are found using a PCR analysis and or southern blotting

(Black offspring = founders)

5. Founders can then be further analyzed for affects of that transgene

Methods Continue…Transgenic Mice Technology Part 1

Transgenic Mice Technology Part 2

Why create transgenic mice?

Transgenic Mice aids study of Pancreatic Cancer

•Transgenic Mice have:• Aided the study of Alchemies Disease• Aided in study of pancreatic cancer

• Acted as disease models • Tested toxicants • Produced specific proteins helpful in

biotechnology

Pros and Cons • Pros

– Cheap– Short generation time– Defined genes

• Cons – Ethical Concerns

• Are the animals treated with respect?– Environmental Issues

• What if a genetically modified animal gets loose?– Religious Views

• Do we have the right to act as God?

Animation And Picture Credits• Experiments." Experiments. Web. 23 May 2012.

<http://student.biology.arizona.edu/honors2005/group07/Experiments.html>.• "Transgenic Mice." Transgenic Mice. Web. 23 May 2012.

<http://www.accessexcellence.org/RC/VL/GG/transgenic.php>. • Creating Transgenic Mice: On-Line Self-Study." Human Development. Professor Danton O'Day.

Web. <http://www.utm.utoronto.ca/~w3bio380/pdf/Creating%20Transgenic%20Mice.pdf>.• "Transgenic Mouse Animation." Transgenic Mouse Animation. Web. 23 May 2012.

<http://spine.rutgers.edu/cellbio/assets/flash/transgen.htm>.• Transgenic Animals. 13 June 2011. Web.

<http://users.rcn.com/jkimball.ma.ultranet/BiologyPages/T/TransgenicAnimals.html>.• "Fig002jme: Two Methods to Produce Transgenic Mice." Fig002jme: Two Methods to Produce

Transgenic Mice. Web. 23 May 2012. <http://journals.cambridge.org/fulltext_content/ERM/ERM3_12/S1462399401002952sup004.htm>.

• Proneural. "The Science of Transgenic Technology Part 2 of 2." YouTube. YouTube, 25 Apr. 2007. Web. 23 May 2012. <http://www.youtube.com/watch?v=EFNtTfhhQ70>.

• Proneural. "The Science of Transgenic Technology Part 1 of 2." YouTube. YouTube, 25 Apr. 2007. Web. 23 May 2012. <http://www.youtube.com/watch?v=ujZHrR1mro8>.