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第八章 克隆基因的表达 Expression of Cloned Genes. Gene Organization. Gene Expression and Regulation Levels. The lac Operon. Structure and expression of the mammalian β-globin gene. Transcription and Translation. Transcription in Prokaryotes. Structure of a typical promoter in E.coli. - PowerPoint PPT Presentation
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第八章 克隆基因的表达Expression of Cloned Genes
Gene Organization
Gene Expression and Regulation Levels
The lac Operon
Structure and expression of the mammalian β-globin gene
Transcription and Translation
Transcription in Prokaryotes
Structure of a typical promoter in E.coli
S-D Recognition Sequences in Prokaryotic mRNAs
Termination of RNA Chains
Expression Vector
Secretion Cloning Vector
Fusional ExpressionVector
Factor Xa---Activated form of factor x that participates in both the intrinsic and extrinsic pathways of blood coagulation. It catalyses the conversion of prothrombin to thrombin in conjunction with other cofactors.
Conserved Sequences in Promoters
Distance of Structure Genes and Promoters
Gene expression
level and secondary
structure of mRNA
Alteration of the Distance between Gene and Promoter
Translation Reading Frame
Construction the Reading Frame
Induced Expression of Gene
• Chemical---lac promoter and IPTG
IPTG 即 Isopropyl β-D-1-thiogalactopyranoside ,也称 Isopropyl β-D-thiogalactoside ,中文名为异丙基 -β-D- 硫代半乳糖苷。分子式为 C9H18O5S ,分子量为 238.30 。
-IPTG, cell growth
+IPTG, target gene expression
Induced Expression of Gene
• Physical---λPL and temperature sensitive(λcI875 lysogen,32 ,42℃ ℃)
Induced Expression of GenePromoter: λPLλPR Terminator: rrnBT1T2Supression: 32 Gene expression: ℃ 42 ℃
Transcription
and Translation
in SV40 Virus
SV40 Virus Vector
SV40 Virus
Vector and
COS Cells
Expression Vectors for Mammalian Cells
Gene Expression
in Mammals
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