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EXPRIMENTAL DESIGN
SOAPNUT OIL-Biofuel from a non-edible plant
Equipments: separating funnel, pots, a cup, syringe, Bunsen
burner, hammer.
Reagents: soapnut fruit, water, heat source, lead acetate, HCl,
hydrogen sulphide and alcohol.
Temperature=1700cPressure=1.6atm
my productionI will first harvest the seeds,. Then after harvesting them, I will collect the seeds. I will
then crack them using the hammer to separate the nuts from the shells. From there, I will
cold press(temperature is controlled so that it does not exceed 120 deg. F) the nuts for
the oil and I will obtain about 1.5 grams of the oil.
After extracting the oil, I will then transfer it into a container
using a cup.
EXTRACTIONI will use the process of water extraction to get my oil by boiling the nuts in
water at a very high temperature and pressure such that the oil will float on
the water and then I can obtain the oil from the water by using a separating
funnel.
Melting point=105-107deg.CStorage temp= room tempMolecular formula :C27H42O3
From there, I can then extract my main molecule which then helps in the cure of the asthma found in the oil, that is saponin. The saponin could be extracted by process of chemical extraction by fusing( convert it into a liquid) it at 95deg.C.
The saponin is prepared by adding excess lead acetate to the oil, then afterwards, some of the lead is removed using hydrogen sulphide. HCl is then added at 40deg.C and white crystals of saponin are obtained. The saponin obtained is not pure and so can be
purified by dissolving in alcohol
I will first lyophilize the saponin, that is convert it into the a
gas. I will then induce the gas into mice using a syringe and
study it inhibitory activity against toxic compounds that cause
respiratory problems like 5-lipoxygenase(5-Lo),
cyclooxygenase(COX), Leukotriene B4(LTB4) and Nitric Oxide
synthase(NOS) through the action of the enzymes found in it
like serum glutamic pyruvic transaminase (SGPT), serum
glutamic oxaloacetic transaminase (SGOT) and alkaline
phosphatase (ALP) elevate in the blood, and hepatic
glutathione and superoxide dismutase (SOD) decrease
Testing the saponin-invivo
Expected Results
After inducing the gas into the mice, I expect that immediately
after an hour, it should produce about 90% inhibitory effect on the
toxic compounds that is, it should start inhibiting them immediately
after 1 hour till when they can no longer act.
Therefore I expect that if it is used in humans, it might reduce the
level of asthma by about 85%.
http://
www.ijptonline.com/wp-content/uploads/2009/10/2201-2214.pdf
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3414376/
http://www.chemicalbook.com/ChemicalProductProperty_EN_CB9393639.htm
http://pubs.acs.org/doi/pdf/10.1021/ie50354a012
References
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