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“Sushi-gate 2008”: High School students apply DNA barcoding
to fish sold in their NYC neighborhood,
discover one-quarter is mislabeled
Research report by students Kate Stoeckle and Louisa Strauss
published in Pacific Fishing September 2008
Can DNA name this fish?
The New York Times August 21, 2008, page 1
Chosun Ilbo (Korean Daily News)circulation 2.2 million
“Sushi-gate” draws wide interest
CBS Early Show August 23, 2008
Barcode of Life Database (BOLD): www.barcodinglife.org
BOLD project “Fish Market Survey”
Analyzing Sushi-gate fish samples
DNA sequencing at University of Guelph
1. Collect specimens 2. Prepare samples 3. Record data 4. Send for analysis
5. Upload specimen, sequence data on BOLD
5. Search databases for identical or closely-related sequences
6. Learn more on FishBase, other
Mislabelings were as more expensive or more desirable fish
“Red Snapper” DNA ID: Nile PerchRed Snapper
to scale
Among 60 items tested:-Nile Perch (Africa), Lavender Jobfish (SE Asia), Slender Pinjalo (SE Asia), and Acadian Redfish (N Atlantic) sold as “Red Snapper”-Mozambique Tilapia sold as “White Tuna”-Smelt Roe sold as “Flying Fish Roe”-Caribbean Spotted Goatfish sold as “Mediterranean Red Mullet”-White Bass (farmed freshwater fish) sold as “Sea Bass”
Mislabeled items were sold in 6 of 10 grocery stores/fish marketsand 2 of 3 restaurants
Range map: FishBase Range map: FishBase
FishBase: D Flescher FishBase: John Casselman
The Trinity SchoolKate StoeckleLouisa Strauss
The Rockefeller UniversityJesse AusubelMark Stoeckle
University of GuelphRobert HannerEugene Wong
Fish Barcode of Life Initiative
Unexplored territory?
DNAHouse: 2009 student project exploring urban environment with DNA barcoding
? ?
Acknowledgments
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