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Part 4: Instructor Guide
Introduction
Active attentiono Instructor will show video clip of microscopes used in movies (Outbreak and
Contagion). Also, show images and videos of cell specimens under a microscope,
from the internet (See Technology Tools 3 and 4). Have students discuss the
importance of microscopes. (Outbreak minutes 23-28).
Establish Purposeo Introduce the learning goal: Learners will demonstrate their ability to identify
parts of the microscope and their functions, as well as, set up, use, and clean up a
microscope safely and correctly.
o Have students write learning objectives to meet the goal. Discuss with the classas a whole to create objectives. Lead the students to additional, less obvious,objectives, such as those for total magnification. As a class, develop one
comprehensive list.
Stimulate motivation and attentiono Have students complete a writing prompt in their notebooks (Appendix D).
Prompt: List five things you would like to see using a microscope andwhy?
Preview learning activity/activity overviewo Bring out the microscopes and give students note cards with microscope parts and
note cards with part functions (Appendix C). Have students work in pairs tobegin matching cards and identifying these parts on the microscopes. Instructor
should check for accuracy once partners feel their matches are correct.
Body
Recall prior knowledgeo Have students complete a KWL chart (Appendix E). Students should fill in the
what they know and what they want to know portions. Once they have time
to complete their list, have students compile their lists together at the board for
everyone to see.
Present Information and exampleso Instructor should review parts of the microscope using microscopes themselves
and the handout picture of a microscope for students to fill in the parts duringinstruction (Appendix F).
o Instructor should demonstrate proper technique when carrying, using, andcleaning up the microscope. Show YouTube video How to use a microscope.Discuss the following:
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Carry with one hand on arm, one hand under base. Check cord for frays; wrap cord up when finished Keep microscope away from edge of table. Never slide microscope across table. Pick it up and place it where needed. Place low objective into position when finished. Raise objective to highest level before cleaning up.
o Present the principle for finding total magnification. Total Magnification = eyepiece X objective
o Remind students that not all microscopes have the same power for all objectives,the most commonly used may include 4x, 10x, 40x, and 100x. Give students a
few practice problems on the board.
Focus Attentiono Allow for question and answer period for material presented so far.o Give students the handout, Procedure Checklist (Appendix G). Instructor shows
demonstration of how to properly use a microscope from set up, to use, to cleanup. Use the checklist and walk through the procedure; show students what is
expected for each step.
o Allow students to begin hands on investigation of microscopes. Studentsshould review parts and functions with partners. Instructor should circle aroundclassroom to address questions, provide feedback, and direct students to the next
step of their procedure checklist. All students should demonstrate to the
instructor that they can find and focus the slide properly.
Learning Strategieso Procedural Knowledge: Students should use procedure checklist. Have students
view unbreakable samples, not glass slides. Students may look at yarn, hair, or
newspaper. If students have any other appropriate specimens they would like to
look at they may do that as well, such as bug antennae or wings. Allow forinquiry investigation among students.
o Students must complete the following in their notebooks: For each object viewed:list observations, create one drawing of the specimen for each microscope power
used (low, medium, and high), and find total magnification for each objectiveused (Appendix D).
Provide Practiceo Have students use procedure checklist and begin practice focusing glass slides or
plastic slideswhichever is more appropriate with the students. Walk around
classroom to address questions, provide help, and feedback.
Provide Feedbacko While students investigate slides, observe technique and provide feedback.
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Conclusion
Summarize and Reviewo Complete the last portion of the KWL chart, what students learned (Appendix E).o As a class, address the objectives the students created at the beginning of the
lesson. Have students reflect in their notebooks on the following questions: Didwe meet all of the objectives? Why or why not? How were the specific objectivesmet, give examples?
Transfer Learning/ Enhance Transfero Have students complete the following notebook entry: How can this knowledge
be used in the future? During this time, you may show a video from discovery
learning on careers that include the use of microscopes.
Remotivate and close/ Provide remediation and closureo Inform students of how they will be assessed: pencil/paper test and a practical
exam demonstrating how to focus a slide and find total magnification. Givestudents the rubric (Appendix H) they will be graded with, go over this with the
class.
o Allow students time to review with partners and time to practice using themicroscope and meeting the rubric requirements.
o Remind students they will not be allowed to use their checklist on the test. Conduct Assessment Evaluate
o Give students the written assessment (Appendix I). During this time, pullstudents to the side, one by one, to give the practical exam. Grade students, usingthe rubric, as they demonstrate how to set up a microscope, focus a slide with allthree powers, and clean up the microscope.
Feedback and Remediationo Give students feedback on their assessments. This should include written
feedback on the exams and verbal feedback to address questions from both
exams.
o If students require remediation, provide them with extra practice and one on onehelp. Also, provide them with some of the websites below and give appropriate
assignments, which will address the areas they need more help with.
o Allow students to seek individual help using microscopes at a designated time(before school, lunch, after school).
See additional resources for extension and remediation activities in the appendices.
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Part 5: Learner Content
5a. Learning Materials
Student Materials Justification and Timeline
1. KWL Chart- Appendix E
The KWL chart will be used during the introduction and
conclusion to this lesson. Students will complete the K
portion (What they already Know) after watching theoutbreak intro and discussing how microscopes are used
every day. This will allow students to identify what they
know and what they Want to know before the lesson. At
the end of the lesson, students will complete the L portionof the chart (What they have Learned).
2. Flashcards- Appendix C
Students will create flashcards as their first activity. They
will write the microscope part on one side, and place a
picture and the function on the back side. Students willrefer to these during the entire lesson. They will also peer
quiz each other on these cards to study for their final
assessment.
3. Notebooks Prompts-Appendix D
Students will complete a variety of notebooks prompts
throughout the lesson.
4. Blank Microscope Image-Appendix F
Students will fill in the image with the missing parts as theyreceive instruction during the body of the lesson.
5. Rubric for finalassessment- Appendix H
Students will be given the rubric that will be used to gradethem on their practical exam. This will be given at the
conclusion and wrap up of the lesson for students to use as a
study tool. The practical exam will have students set up themicroscope, focus a slide correctly and clean up the
microscope. They will also be asked to find total
magnification.
6. Procedure Checklist-Appendix G
This is an informational sheet for students to refer back to
when they are unsure of what to do next. It will be in a
checklist form and will direct students to the correct steps totake when focusing a slide.
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http://www.digitalsmicroscope.com/compound-light-microscope-labeled-3/http://www.digitalsmicroscope.com/compound-light-microscope-labeled-3/http://www.digitalsmicroscope.com/compound-light-microscope-labeled-3/http://www.digitalsmicroscope.com/compound-light-microscope-labeled-3/http://c/Users/Jen/Desktop/Microscope%20Rubric.xlsxhttp://c/Users/Jen/Desktop/Microscope%20Rubric.xlsxhttp://c/Users/Jen/Desktop/Microscope%20Rubric.xlsxhttp://c/Users/Jen/Desktop/Microscope%20Rubric.xlsxhttp://procedure%20checklist.docx/http://procedure%20checklist.docx/http://procedure%20checklist.docx/http://procedure%20checklist.docx/http://procedure%20checklist.docx/http://procedure%20checklist.docx/http://c/Users/Jen/Desktop/Microscope%20Rubric.xlsxhttp://c/Users/Jen/Desktop/Microscope%20Rubric.xlsxhttp://www.digitalsmicroscope.com/compound-light-microscope-labeled-3/http://www.digitalsmicroscope.com/compound-light-microscope-labeled-3/7/28/2019 503 Instructor Guide and Learner Materials.pdf
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5b. Formative and Summative Assessment Materials
Formative and Summative
Assessment Materials Justification
1. Microscope Practical ExamRubric- Appendix H
This is the rubric that will be used when students perform
their practical exam. They will be asked to set up the
microscope, focus a given slide correctly, and clean up themicroscope. They will also be asked to find total
magnification. This addresses objectives 2,3,4,5,6,7 and
11.
2. Microscope Written Test-Appendix I
This is the summative assessment that will be used at the
end of this lesson. This addresses objectives 1, 4, 5, 8, 9,
and 10.
1. Is the timeline appropriate and achievable?2.
Do you have any other comments or suggestions about these materials?
Appendices
A. Learner AssessmentMicroscope knowledge
1. Name three things you can see using a microscope?2. Describe the proper way to carry a microscope:3. When should you use the coarse (large) adjustment knob?4. When should you use the fine (small) adjustment knob?5. If you are unable to locate the specimen on the slide, what should you do?6. How do you find total magnification (what x what)?7. If the eyepiece is 10x and your objective is 10x, what is the total magnification?8. True or False You should always switch from low directly to high power.9. True or False The fine adjustment knob should only be used on high power.10.True or False Coarse adjustment knob should never be used on high power.
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http://c/Users/Jen/Desktop/Microscope%20Rubric.xlsxhttp://c/Users/Jen/Desktop/Microscope%20Rubric.xlsxhttp://c/Users/Jen/Desktop/Microscope%20Rubric.xlsxhttp://c/Users/Jen/Desktop/Microscope%20Rubric.xlsxhttp://c/Users/Jen/Desktop/Microscope%20Test.docxhttp://c/Users/Jen/Desktop/Microscope%20Test.docxhttp://c/Users/Jen/Desktop/Microscope%20Test.docxhttp://c/Users/Jen/Desktop/Microscope%20Test.docxhttp://c/Users/Jen/Desktop/Microscope%20Test.docxhttp://c/Users/Jen/Desktop/Microscope%20Test.docxhttp://c/Users/Jen/Desktop/Microscope%20Rubric.xlsxhttp://c/Users/Jen/Desktop/Microscope%20Rubric.xlsx7/28/2019 503 Instructor Guide and Learner Materials.pdf
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11.With the microscope image and part labeled, please tell me what each parts function orjob is? (Done verbally during interview).
B. Student Interview Questions
1. What is your name?2. What is your age? And grade level?3. What is your ethnicity?4. Where are you from?5. Do you like science?6. Have you used microscopes in this class? What did you do?7. Do you feel confident in using a microscope?8. Have you used microscopes before this class? When (age or education level)? What did
you look at?
9. Do you have any questions about the microscope that havent been answered or anythingelse you are wondering about (in regards to the microscope)?
10.Do you think you will use microscopes in the future (school or career)?11.How do you learn best?
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C. Microscope Flashcards
Create Flashcards with these parts and functions
Microscope Parts Microscope Functions
Eyepiece (Ocular Lens) Lens that you look through to view the slide
Body Tube Connects the eyepiece to the objective lenses
Revolving Nosepiece The part that holds the objective lenses and rotates them
Low Power Objective Lens used to magnify the slide, lowest setting
Medium Power Objective Lens used to magnify the slide, medium setting
High Power Objective Lens used to magnify the slide, highest setting
Coarse Adjustment Knob Focuses the slide on low power ONLY.
Fine Adjustment Knob Focus the slide on low and high power, show small details.
Base The bottom of the microscope, used for supportArm Supports the tube and connects it to the base
Stage The platform where you place your slide
Diaphragm Rotating disk under stage that changes the amount of light
Light Light source that illuminates the slide
Stage clips Holds the slide on the stage
D. Notebook Prompts
Microscope Notebook Entries
1. List five things you would like to see using a microscope and why?2. Follow the procedure given and complete the following in your notebook. View 4 objects
without using glass slides. For each object viewed: List observations, draw the specimen
on two powers and identify the total magnification for each power used.
3. Follow the procedure given and complete the following in your notebook. View 5prepared slides. For each object viewed: List observations, draw the specimen on twopowers and identify the total magnification for each power used.
4. Did we meet all of the objectives? Why or why not? How were the specific objectivesmet, give examples?
5. How can the knowledge you have gained be used in the future?
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E. KWL chart
K W LWhat do you already KNOW
about microscopes?
What do you WANT to know
about microscopes?
What have you LEARNED about
microscopes?
F. Blank Microscope Image
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G. Procedure Checklist
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Procedure Checklist
Retrieve microscope from storage.o Carry microscope properly: one hand on arm, one hand under base.
Place in center of lab table. Plug in microscope and place cord out of the way. Turn on the light source. Check to make sure the low power objective is clicked into place. Place slide on the stage and secure it into place with the stage clips. With your eye looking through the eyepiece, use the coarse adjustment knob to bring the objective
closer to the stage.
Adjust the light source if necessary. Continue to use the coarse adjustment knob until the slide comes into focus; adjust to the best
possible view.
Use the fine adjustment knob to better define the image.Focusing on medium power
First focus the slide on low power by following the above steps. Without touching the slide or coarse adjustment knob, click the medium power objective into
placeas you do this, tilt your head and watch to make sure the objective will not crush the slideon the stage.
Now, use ONLY THE FINE FOCUS adjustment knob to bring the slide into focusFocusing on high power
First focus the slide on low power and medium power by following the above steps. Without touching the slide or coarse adjustment knob, click the high power objective into placeas
you do this, tilt your head and watch to make sure the objective will not crush the slide on thestage.
Now, use ONLY THE FINE FOCUS adjustment knob to bring the slide into focus
When finished, use the coarse adjustment knob to raise the objectives up to the highest positionand click the low power objective into place.
Unplug and wrap the cord around the base of the microscope. Cover and return to storageproperly.
If you are unable to view the slide try the following:
Refocus using low power again. Adjust the light. Move the slide on the stage to make sure it is centered. Clean the slide. Ask the teacher for help.
H. Rubric
Total Magnification: Multiply
eyepiece power times the
objective power
Examples:
10x X 4x = 40x10x X 10x = 100x
10x X 40x = 400x10x X 100X =
1000x
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Microscope Assessment Rubric
ADVANCED PROFICIENT BASIC BELOW BASIC
4 3 2 1
Microscope Parts
Student correctly
identifies 14 of 14
microscope parts.
Student correctly
identifies 10-13
microscope parts.
Studentcorrectly
identifies 5-9
microscope
parts.
Student correctly
identifies 4 or less
microscope parts.
Microscope Setup
Student correctly
carries microscope
with one hand on arm
and one hand under
base; plugs in cord and
turns on light/power,
places microscope in
center of table, and
stores cord awaysafely.
Student correctly
performs 3 out of 4
steps in the sequence.
Student
correctly
performs 2 out
of 4 steps in the
sequence.
Incorrect carrying
procedure. Hazardous
set up. 1 of 4 listed in
the sequence.
Focusing Slide
Student uses stage
clips to mount slide,
adjusts eyepiece,
focuses using lowest
magnification and
coarse focus before
moving to fine focus
and higher
magnification.
Student correctly
performs 3 out of 4
steps in the sequence.
Student
correctly
performs 2 out
of 4 steps in the
sequence.
Student correctly
performs 1 out of 4
steps in the sequence.
Total
Magnification
Student identifies the
magnification of the
eyepiece and
objectives; total
magnification on low
power, medium
power, and high
power.
Student correctlyperforms 3 out of 4
steps in the sequence.
Student
correctlyperforms 2 out
of 4 steps in the
sequence.
Student correctlyperforms 1 out of 4
steps in the sequence.
Microscope Clean
Up & Storage
Student correctly
stores microscope on
lowest power,
switches off power
before unplugging
when appropriate,
wraps cord, and
carries with 2 hands to
store.
Student correctly
performs 3 out of 4
steps in the sequence.
Student
correctly
performs 2 out
of 4 steps in the
sequence.
Student correctly
performs 1 out of 4
steps in the sequence.
Total________/ 20
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I. Summative Assessment
Microscope Test
Match the microscope part with its function
1. ______ Eyepiece A. Focuses slide on all powers2. ______ Body tube B. Holds the slide in place on stage3. ______ Stage C. Bottom of microscope, provides support4. ______ Stage Clips D. Turns the objectives into place5. ______ Arm E. Illuminates the slide to see it better6. ______ Base F. Lens that magnifies the slide the least7. ______ Low Power Objective G. Projects image between the objectives
and eyepiece
8. ______ Medium Power Objective H. Focuses the slide on low power only9. ______ High Power Objective I. The slide is placed on this part10.______ Coarse Adjustment Knob J. Used to carry the microscope at the side11.______ Fine Adjustment Knob K. Adjusts the amount of light12.______ Light L. Lens magnifying the slide13.______ Diaphragm M. Lens that magnifies the slide the greatest14.______ Revolving Nosepiece N. Lens you look though directlyShort Answer
1. Which objective must be in place when you put away the microscope?2. Describe how to carry the microscope?
3. Which focusing knob do you first use when you begin looking at a slide?4. How much are you magnifying something when you are using 10x oculars and the 40x
objectives?
5. How much are you magnifying something when you are using 10x ocular lens and the 10xobjective?
6.
How much are you magnifying something when you using 10x oculars and the 4xobjectives?
7. Why is it important to use the coarse and fine adjustment knobs carefully? Explain indetail.
**The picture from Appendix F will also be used on this assessment.
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