ANSWERS FOR QUIZ

ANSWERS FOR QUIZ

1.7602. 2453. 16 spots 4.142/760 x 100 i.e.18.68%5. 71-26 = 45.6. 20 spots7. 20/760 x 100 i.e.2.63%8. 47-12 = 35.9.(i) Spot ID: 2082 pI : ~4.53 Mol. weight : 24.9 kDal

(ii) Spot ID: 1999 pI : ~4.90 Mol. weight : 75.2 kDal

Set-1. Answers to Bacterial gel

Supportive material

Project Name Bacterial sample 2D-Gels for Analysis.Project Creation Date ………………Author …………………Description Bacterial sample Control vs Drug treated

GroupsControl(1 image)780spots transferred from fused image96 spots cancelled

Drug Treated(1 image)780 spots transferred from fused image96 spots cancelled

Vector Matching :245

1. Project Summary:

4.00 4.25 4.50 4.75 5.00 5.25 5.50 5.75 6.00 6.25 6.50 6.75 7.00

80

75

70

65

60

55

50

45

40

35

30

25

20

15

10

5

pI index

Mol

ecul

ar w

eigh

t (kD

al)

3.Figure showing Molecular weight to pI

85

pI : ~4.53 Mol. weight : 24.9 kDal

pI : ~4.90 Mol. weight : 75.2 kDal

No.

of s

pots

sho

win

g up

regu

latio

n

Up regulation

No. of folds(and above) No.of spots

2 142

4 71

6 36

8 26

10 16

12 12

15 10

4.1.Differential expression : Up regulation

Up regulationx Folds or above

No.

of s

pots

sho

win

g up

regu

latio

n

Down regulation

No. of folds(and above) No.of spots

2 174

4 47

6 20

8 12

10 8

12 4

15 1

Down regulationx Folds or above

4.2.Differential expression : Down regulation

1. 349 spots2. 1093. 16 spots4. 69/349 x 100 i.e.19.77%5. 33-16 = 17 6. 28 spots7. 48/349 x 100 i.e.13.75 %8. 83-28 = 559.(i) Spot ID: 2082 pI : 5.28 Mol. weight : 44.8 kDal

(ii) Spot ID: 1999 pI : ~5.26 Mol. weight : 65.2 kDal

Set -1. Answer of Serum sample

Supportive material

Project Name Serum sample 2D-Gels for Analysis.Project Creation Date …………………………………Author ……………….Description Healthy vs diseased patient

GroupsControl(1 image)Control - Healthy person349spots transferred from fused image97 spots cancelled

Diseased (1 image)P.falciparum diseased serum sample349 spots transferred from fused image97 spots cancelled

Vector Matching : 109

Project Summary:

4.00 4.25 4.50 4.75 5.00 5.25 5.50 5.75 6.00 6.25 6.50 6.75 7.00

80

75

70

65

60

55

50

45

40

35

30

25

20

15

10

5

pI index

Mol

ecul

ar w

eigh

t (kD

al)

85

pI : 5.28 Mol. weight : 44.8 kDal

pI : 5.26 Mol. weight : 65.2 kDal

3.Figure showing Molecular weight to pI

No.

of s

pots

sho

win

g up

regu

latio

n

Up regulation No. of folds(and above) No.of spots

2 693 474 395 338 21

10 1615 1520 1025 850 375 1

Up regulationx Folds or above

Differential expression : Up regulation

Down RegulationNo. of folds(and above) No. of spots

2 83

3 48

4 37

5 28

7 13

10 11

15 5

30 2

No.

of s

pots

sho

win

g up

regu

latio

n

Down regulationx Folds or above

4.2.Differential expression : Down regulation