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Supporting Information
Semi-automated set-up for exhaustive micro-electromembrane extractions of basic drugs
from biological fluids
Miloš Dvořáka, Knut Fredrik Seipb,c, Stig Pedersen-Bjergaardb, Pavel Kubáňa*
a Institute of Analytical Chemistry of the Czech Academy of Sciences, v. v. i., Veveří 97, CZ-
60200 Brno, Czech Republic
b School of Pharmacy, University of Oslo, PO Box 1068, Blindern, NO-0316 Oslo, Norway
c Aker Biomarine Antarctic AS, PO Box 496, NO-1327 Lysaker, Norway
Dr. Pavel Kubáň, e-mail: kuban@iach.cz, Tel: +420 532290140, Fax: +420 541212113, Institute
of Analytical Chemistry of the Czech Academy of Sciences, v. v. i., Veveří 97, CZ-60200 Brno,
Czech Republic
Figure S1. Two experimental set-ups used for connecting the microextraction unit with semi-
automated liquid handling system during performance characterization. Set-up I shows detailed
connections of the system without µ-EME electrode and Set-up II with µ-EME electrode.
Set-up I constitutes the simplest possible connection of syringe pump and microextraction unit
whereas set-up II demonstrates connection necessary for semi-automated µ-EMEs, which also
includes the μ-EME electrode. In the Set-up II, two wires (Cu, 150 μm OD and Pt, 250 μm OD)
were examined as the μ-EME electrode, which was stretched through a hole in the silicone
tubing and then threaded between the silicone and the Tygon tubing. Leak-proof connections
between the pump and the extraction units were obtained with the Set-up I and with the Set-up II
using the thinner electrode, whereas leaks were detected for the thicker electrode. This led to
poor precision and accuracy of withdrawn liquid volumes. In addition, no differences in µ-EME
performance were observed for the two electrode materials and for these reasons Cu wire was
used as anode. The Cu wire at the anodic side of the µ-EME system was replaced with new Cu
wire regularly after two weeks of use.
Cu electrode
Silicone tubing
Needle Tygon tubing
Set-up II
PP pipette tip
PP pipette tipTygon tubing
Needle
Set-up I
2
Figure S2. Real-time monitoring of electric currents during µ-EMEs of standard solutions and
biological fluids spiked with different concentrations of nortriptyline, papaverine and
haloperidol. A. Electric currents for 0 – 20 mg/L of the drugs in standard solution. B. Electric
currents for non-spiked urine/plasma and urine/plasma spiked with 10 mg/L of the drugs. µ-EME
conditions: acceptor, 25 mM HCl (1.3 µL); FLM, ENB (2.5 µL); donor, standard solution, urine
and plasma non-spiked/spiked with the three drugs in 10 mM HCl (1.3 µL); extraction voltage,
150 V; extraction time 10 min.
3.53.02.52.01.51.00.50.0
electr
ic cu
rrent
(A)
1086420extraction time (min)
spiked plasma 10 mg/L blank plasma spiked urine 10 mg/L blank urine
3.53.02.52.01.51.00.50.0
electr
ic cu
rrent
(A)
1086420extraction time (min)
20 mg/L 10 mg/L 5 mg/L 1 mg/L 0 mg/L
B
A
3
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