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Assessing Vertical Microbial Transport Assessing Vertical Microbial Transport Assessing Vertical Microbial Transport Assessing Vertical Microbial Transport

in Bedrock Well Columnin Bedrock Well Columnin Bedrock Well Columnin Bedrock Well Column

Eric J. Raes, P.E., LSRP

Engineering and Land Planning Engineering and Land Planning

Dora Ogles, Brett Baldwin, Kate Clark & Anita Biernacki

Microbial Insights Inc.

Kerry Sublette, Ph.D., Kate Key

University of Tulsa

We We We We knowknowknowknow

That Hydrologist Like to

Draw Arrows

We We We We knowknowknowknow

That Chemist Like using

Colors

But what do we know about microbial But what do we know about microbial But what do we know about microbial But what do we know about microbial

migration?migration?migration?migration?

Let’s ask a friend.

But what do we know about microbial But what do we know about microbial But what do we know about microbial But what do we know about microbial

migration?migration?migration?migration?

Let’s ask a friend.

Microbial Release and Transport StudyMicrobial Release and Transport Study

0.100.120.140.160.180.20

met

er/d

ay

0.000.020.040.060.080.10

0.5 - 1 mm 0.25 - 0.5 mm 0.05 - 0.25 mm

met

er/d

ay

Sand Size Range

Pilot Study Results - IW-1 and Piezometers

PZ-4

IW-1

PZ-3PZ-2 PZ-1

Radius of Influence?Radius of Influence?

0.6016:30 05:42 18:54 08:06 21:18 10:30

-0 .100.080.260.440.620.80

DO

(mg

/L)

16:30 05:42 18:54 08:06 21:18 10:300.5100.5960.6820.7680.8540.940

Sp

Co

n(m

S/c

m)

16:30 05:42 18:54 08:06 21:18 10:3015.2015.3815.5615.7415.9216.10

Te

mp

(C)

WP-1.DAT

16:30 05:42 18:54 08:06 21:18 10:30-444.0-330.6-217.2-103.8

9.6123.0

OR

P(m

V)

16:30 05:42 18:54 08:06 21:18 10:305.806.046.286.526.767.00

pH

16:30 05:42 18:54 08:06 21:18 10:30

0.600.740.881.021.161.30 D

ep

th(m

)

DateTim e(M/D/Y )

12/07/11 12/14/11 12/20/11 12/27/11 01/02/12 01/09/12

Star Pupils

••

GW Flow

Anaerobic Bioreactors, the same but different

100

1,000

10,000

100,000

8

10

12

14

16

(Cel

ls/m

L)

So

lven

t C

on

cen

trat

ion

s(u

g/L

)

MW-1 Solvent Concentrations vs. Time

0

1

10

0

2

4

6

(Cel

ls/m

L)

So

lven

t C

on

cen

trat

ion

s

Date

Tetrachloroethene Trichloroethene Vinyl Chloride cis, 1,2 DCE Dehalococcoides (DHC) tcaA Bav1

Legend

Vertical Migration AssessmentVertical Migration Assessment

•• Chlorinated SolventsChlorinated Solvents

•• Deep water column with multiple fracturesDeep water column with multiple fractures

•• Non cooperative geoNon cooperative geo--chemical conditionschemical conditions

MultiplMultiple e FracturFractureses

ImplementationImplementation

TreatmentTreatment

Chemical and GeoChemical and Geo--Chemical TrendsChemical Trends

3/18/2015 6/22/2015 9/17/2015

PCE 28.7 18.6 1.7

TCE 1230 676 7.5

cDCE 133 23.2 61.5cDCE 133 23.2 61.5

VC 0 0 1.9

Ethane 0 0 0

iron 316 103 8260826082608260

SO4 51.4 46.2 19.6

Methane 0 0.34 2040204020402040

Microbial Ground Water ResultsMicrobial Ground Water Results

1.00E+03

1.00E+04

Microbial Populatons in GW

1.00E+00

1.00E+01

1.00E+02

3/1/2015 4/1/2015 5/1/2015 6/1/2015 7/1/2015 8/1/2015 9/1/2015

DHC BAV1 DHBt

DHC vs DepthDHC vs Depth

Chemical and GeoChemical and Geo--Chemical TrendsChemical Trends

Chemical and GeoChemical and Geo--Chemical TrendsChemical Trends

ImplementationImplementation

TreatmentTreatment

Donor WarsDonor Wars

Questions?Questions?