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Dec 9th, 2013
Nagib M. WARD, Executive VP
BIO PACIFIC RIM SUMMIT
DEINOCOCCUS
A NEW SCIENTIFIC AND TECHNOLOGICAL OPPORTUNITY
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• Deinove holds the largest collection of Deinococci in the world (6,000 strains)
• A proprietary technological platform (16 patents) with validation through a POC (DEINOL)
Num
ber
of public
ations
200
000
100
000
300
000
Microorganisms
NUMBER OF PUBLICATIONS PER
GENUS
Num
ber
of pate
nts
4 000
0
8 000
Microorganisms
NUMBER OF PATENTS PER
GENUS
Escherichia coli
Saccharomyces
cerevisiae
Deinococcus
Deinococcus
geothermalis
The world pioneer in deinotechnologies and their IP.
DEINOVE
D. radiodurans discovered in 1956, by accident.
Pr. Miroslav Radman: DNA repair research.
Industrial tool: Deinove creation in 2006.
DEINOCOCCUS GENUS
DEINOVE
CORPORATION KEY DATES
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2009 2007 2008
Inception, co-founders Pr Miroslav RADMAN Dr Philippe POULETTY
2013 2006 2010
DEINOL
EC1
2011 2012
OSEO funding (€1.57 m)
Jacques BITON CEO
IPO (€12 m) Partnership with TEREOS Partnership with VTT OSEO funding (€1.4 m)
OSEO funding (€6 m)
Deinobank (6 000 strains) Initiation of DEINOL program
Deinobank (25 strains)
Proprietary technological platform Initiation of DEINOBIOTICS program
Initiation of DEINOCHEM program
DEINOL EC2 (POC) THANAPLAST™ program
DEINOBIOTICS SAS
OSEO funding (€1.15 m) Market Cap: €53 m 30 FTE
Emmanuel PETIOT CEO Nagib WARD Exec VP
2 5 31 Staff
DEINOVE
Diverse environments have been targeted…
…unusual selection by irradiation
…unusual carbon sources
Deinococcus library (1800 strains) AND diverse library of original strains (4200)
All strains collected according to the international laws (CBD)
Source of original new biocatalysts
6000 STRAINS LIBRARY, MANY UNSUAL
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DEINOVE RESEARCH CENTER
BIOPÔLE EUROMÉDECINE IN MONTPELLIER, FRANCE
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+40 EMPLOYEES 1000 m2
DEINOVE
SSCF PROCESS
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DEINOVE
Operating at 47-52C allows maximizing enzymes at their biochemical optimum, minimizing doses.
INDUSTRIAL ADVANTAGES
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CBP: enzymes are excreted in solutions to boost digestion, accelerate cofermentation
An polyextremophile with remarkable resistance to harsh conditions or toxic substances.
Metabolizes C3s but also
coassimilates C6 and C5s.
Outstanding molecular biology tool to express
specific sequences:
• Enzymes
• Metabolic Pathways
Vast IP opportunities for a largely unknown and
underutilized bacterial strain.
DEINOVE
Higher temperature (~50C):
• Lower infection rates/risks
• Lower enzyme dose by leveraging biochemical optimums Inhibitors/Products resistance:
• Allows utilizing cellulosic sugars
• Maximizes product yields Molecular Transformation:
• Express proprietary sequences.
• Introduce new metabolic pathways CBP/Enzyme release:
• Significantly affect conversion/hydrolysis costs
• Promote optimal process type: SSF IP Opportunities:
• Vastly unfiled IP field.
PROCESS BENEFITS IN INDUSTRY
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Deinococcus ficus Deinococcus aquaticus Deinococcus daejeonensis
Roseomonas aestuarii
Deinococcus marmoris
Rhodococcus corynebacterioides
Kocuria turfanensis
PIGMENTED BACTERIA BIODIVERSITY : PINK, ORANGE AND RED PIGMENTS
3: Feedstock
1: Main Product
2: Co-Product
4: Process
Benefits
DEINOVE
BUSINESS MODEL
DEINOVE
COMMERCIAL STRUCTURE
Deinol
Green Solvents Biofuels
Deinochem
Renewable Chemicals
Nutraceuticals Deinomediation
ThanaPlastTM
Deinobiotics
DEINOVE
ETHANOL
MARKETS AND PRODUCTS
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ADVANCED
BIOFUELS
RENEWABLE CHEMICALS &
INTERMEDIATES
ADVANCED CHEMICALS
SUGARS AND PREBIOTICS
HIGH-VALUE
INTERMEDIATES
BIODIGESTION
THE DEINO WAY TO RENEWABLE CHEMICALS
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DEINOCHEM
Our biochemical platforms leverage Deinoccocus’ advantageous properties in industrial settings:
• The extreme robustness of the bacterial strains
• Their feedstock flexibility and usage efficiency
• The genetic modification efficiency and optimization of
DEINOVE’s strains
These properties are key success factors for DEINOVE’s targeted industrial applications, by: allowing a customization of the platforms for each commercial partner
being independent of either the starting feedstock or pretreatment technology employed
OUR GREEN BIOTRANSFORMING PLATFORM
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HO
O
OHHO
HO
HO
DEINOCHEM
BIOMASS VALUE GENERATION
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C3, C5 & C6 Sugars Conversion
HO
O
OHHO
HO
AcCoA
AcetoAcCoA
hydroxybutyrylCoA
crotonylCoA
butyrylCoA
MalonylCoA
H3C OH
Butanol
H3C CH3
CH3
OH
H3C CH3
HO
H3C
CH2
H2C CH3
CH2
Geraniol Linalol Isoprene
H3COH
O
NH2
OH
O
O
H3C
OH
O
O
H3C CH3
O
HO
O
CH2
H3C OH
H3C CH3
OH
H3C
H3C
CH2
StyrEne
Isobutanol
Butanol
Isobutene
H2C
O
OH
Diaminobutane
Acrylate
NH2H2N
Solvents
Paints
Plastics
Dispersants
Cosmetic
Speciality Chemicals
FERMENTATION PATH
AMINO ACIDS DERIVED PATHWAYS
ISOPRENOÏDS
Renewable
biochemicals
DEINOREDUCTION Nutraceuticals
Solvents
DEINOVE
BIOTRANSFORMATION
Specific molecular biology tools are
necessary to biotransform Deinove’s
strains.
Exact location genetic insertions, or
deletions (KO).
Numerous “genetic cassettes” can be
expressed at high levels.
0
45
90
180
135 225
270
315
Deinococcus main chromosome
Available sites
new sites in progress
Multi-targetting cassette
Specific targetting cassette
DEINOVE
Green chemicals do not
command price premiums in
the market:
Conversion rates and both products
and feedstock prices are key!
ADEME announced its support
of Deinove’s Terpenoids
production ambition:
$8.5MUSD in 2013.
DEINOL CONVERSION RATES
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DEINOCHEM
Terpenoids:
Linalool, Geraniol and B-Pinene
Pathway Intermediates: Isopentene/isopentane Nutraceuticals:
Prebiotics: Xylitol and Fucosylated Sugars
Carotenoids
Green Chemicals:
Succinic Acid
PDO
Adipic Acid
HMD
MARKET OPPORTUNITIES
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DEINOCHEM
DEINOCHEM program • Resources dedicated to the program
(2012 launch/2013 acceleration)
• Promising early work from our laboratories
• Advanced discussions with many world
leading industrials partners
The largest family of natural
substances in the world:
• More than 22,000 isoprene compounds
• Many industrial applications: creams,
fragrance, detergents, anti-cancer agents…
Looking for new production
standards:
• Extraction from plants and chemical
synthesis suffer from low yields and high
production costs
THE WAY TO ISOPRENOIDS
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DEINOCHEM
HIGHER RESISTANCE TO ISOPRENOÏDS
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IC50 (G/L) ISOPRENE GERANIOL LINALOOL b-PINENE
E. coli 7 0,6 4 91,7
S. cerevisiae 8 0,8 4 22,5
D. radiodurans
R1 21 No Inhibition 25 22,5
D. geothermalis
MX6-1E 12 7,8 5 ND
D. geothermalis
MX6-1C 14 No Inhibition 7 ND
D. geothermalis
MC2-2A 14 No Inhibition 7 ND
D. geothermalis
M36-7D 22 0,6 3 91,7
METABOLIZATION TEST (MTT)
DEINOCHEM
DEINOVE is now developing a brand new application platform named DEINOREDUCTION™
focused on the industrial bioreduction of various materials to produce high value renewable
compounds.
The first applications will be focused on the following opportunities:
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Xylitol PDO, Isopropanol
and Butanol
Fucose and fucosylated
prebiotics
DEINOCHEM
C6 derivatives, ideally from a cellulosic carbon source: Adipic Acid: >6BUSD Market
C6 derivative, diamine for polyurethanes, nylon manufacturing.
HMD: >4BUSD Market
C6 derivative, dicarboxylic acid for polyesters, nylon manufacturing.
Terpenoid pathway intermediates as solvents or synthetics:
Isopentane: ~$2.5/Kg
Isopentene: ~$3/Kg
PRODUCTS OF INTEREST
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DEINOCHEM
HIGHER RESISTANCE TO SOLVENTS
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IC50 (G/L) ETHANOL DECANOL SERINOL HEXANOL BUTANOL BUTANOL ACRYLIC HEXANOIC
Gentle shaking Strong shaking ACID ACID
E. coli 43 6 46 4.5 24 89 0.3 0,2
S. cerevisiae 115 ND 93 ND 24 86 0.2 0,3
D. radiodurans
R1 294 7 404 17 96 107 1.2 3.9
D. geothermalis
MX6-1E 126 3
No
inhibition
No
inhibition 240 92 1 3.4
D. geothermalis
MX6-1C 118 4
No
inhibition 22.7 272 ND 0.9 3.6
D. geothermalis
MC2-2A 115 4
No
inhibition 12.3 184 ND 1 ND
D. geothermalis
M36-7D 125 2 ND 11.4 200 ND 1.9 2.6
METABOLIZATION TEST (MTT)
MERCI!!! THANK YOU!!!
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CONTACTS
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Emmanuel PETIOT CEO Tel : + 33 1 42 03 27 37 emmanuel.petiot@deinove.com
Jean-Paul LEONETTI VP R&D Tel : + 33 1 42 03 27 37 jean-paul.leonetti@deinove.com
Nagib M. WARD Vice President, Business Development
nagib.ward@deinove.com
CapTrust Tower, 4208 Six Forks Road, Suite 1000
Raleigh, NC 27609 USA
(O): +1 919-645-5784 (M): +1 609-772-2223 (F): +1 919-825-4801
DISCUSSION SUPPORT
OUR PROPRIETARY TECHNOLOGICAL PLATFORM
THE BEST OF GENETIC ENGINEERING AND BACTERIOLOGY
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ARCHIVING
Library of cryopreserved
strains
Physiology
High-throughput growth assays
Metabolomic
Functional tests Gene expression
measurement
Microbiology
Genomic
PCR Sequencing & Annotation
DEINOBANK (6,000 clones)
Genetic & Metabolic
Engineering
Gene insertion Gene deletion
Sequence optimization
LAB PILOT
INDUSTRIAL PILOT
PRODUCTION
COLLECTION SELECTION OPTIMISATION SCALE UP
LEAD DEINOCOCCUS CANDIDATE
S1 S2 S3 POC
Fermentation engineering
Optimization of culture &
fermentation processes
Industrial engineering
Lab Assays Test &
Integration
Samples collection in
extreme environment
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