Blocking myofibroblast differentiation with an anti-TGFβ...

Blocking myofibroblast differentiation with an anti-TGFβ antibody decreases the induction of higher-order aberrations after photorefractive keratectomy (PRK) in a cat model

Bühren J, Nagy LJ, DiMagistris M, MacRae SM, Huxlin KR

Advanced Physiological Optics Laboratory Department of Ophthalmology University of Rochester Medical Center Rochester, N.Y., USA

Objective

•  Wound healing can compromise the outcome of surface ablation procedures (PRK)

Objective

•  Wound healing effects can compromise the outcome of surface ablation procedures (PRK)

•  TGFβ (Transforming Growth Factor β) plays a pivotal role in corneal wound healing (esp. transformation of keratocytes into myofibroblasts)

Objective

•  Wound healing effects can compromise the outcome of surface ablation procedures (PRK)

•  TGFβ (Transforming Growth Factor β) plays a pivotal role in corneal wound healing (myofibroblast transformation)

•  Hypothesis: “Contractile myofibroblasts contribute to induction of higher order optical aberrations after PRK”

Methods: PRK in cats

•  8 eyes from 5 domestic shorthair cats (felis cattus) underwent PRK for myopia (Planoscan, B & L) -10 D, 6mm OZ

•  follow-up exams: 2, 4, 8 and 12 weeks

treatment eye: anti-TGFβ

(1mg/mL b.i.d.)

control eye: vehicle solution b.i.d.

for 7 days

Methods: aberrometry in awake-behaving cats

•  wavefront sensing (H/S sensor - 300µm lenslet spacing) in fixating cats •  Zernike decomposition (10th order)

–  LOA RMS, coma RMS, SA RMS, residual RMS –  VSOTF

Huxlin, Yoon, Nagy, Porter, Williams. Vis Res 2004;44:2159-69 Nagy, MacRae, Yoon, Cox, Huxlin , J Cat Refr Surg 2007; 33: 1051-1064 Bühren, Yoon, Kenner, MacRae, Huxlin, IOVS 2007; 48: 5806 – 5814

1 mm

Results: wavefront I (LOA)

M [D

]

time [week] 0 4 8 12

-2

0

2

4

6

8 M

J0 [D

]

time [week] 0 4 8 12

-1.0

-0.5

0

0.5

1.0 J0

J45

[D]

time [week] 0 4 8 12

-1.0

-0.5

0

0.5

1.0

J45

treatments controls

LOA: no differences

Results: wavefront II (HOA)

coma

time [week] 0 4 8 12

com

a R

MS

[µm

]

0

0.5

1.0

1.5

time [week] 0 4 8 12

SA

RM

S [µ

m] spherical

0

0.5

1.0

1.5

time [week] 0 4 8 12

resi

dual

HO

A R

MS

[µm

]

0

0.5

1.0

1.5 residual

treatments controls

C ≥2

n

C ±1

n C 0

n

Results: wavefront II (HOA)

coma

time [week] 0 4 8 12

com

a R

MS

[µm

]

0

0.5

1.0

1.5

time [week] 0 4 8 12

SA

RM

S [µ

m] spherical

0

0.5

1.0

1.5

time [week] 0 4 8 12

resi

dual

HO

A R

MS

[µm

] 0

0.5

1.0

1.5

residual

log

BC

VS

OTF

-2.0

-1.6

-1.2

-0.8

-0.4

0 BCVSOTF

time [week] 0 4 8 12

treatments controls

α-TGF treatment group: •  less coma, SA and other HOA

•  better retinal image quality •  less fluctuation

treatments controls

Conclusion

Pharmacological wound healing modulation can affect wavefront aberrations after PRK

Thank you !