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XXI Meeting of the
Portuguese Society of
Animal Pathology
Beyond Routine Diagnosis
XXI Meeting of the Portuguese Society of Animal PathologyBeyound Routine Diagnosis, 20 and 21 of May 2016
XXI Meeting of the
Portuguese Society of
Animal Pathology
Veterinary Pathology :
Beyond Routine Diagnosis
20 and 21 May 2016
Meeting of the Portuguese Society of Animal Pathology Beyound Routine Diagnosis, 20 and 21 of May 2016
1
XXI Meeting of the
Portuguese Society of
Animal Pathology
Veterinary Pathology :
Beyond Routine Diagnosis
20 and 21 May 2016
Abstracts in Conference Proceedings
XXI Meeting of the Portuguese Society of Animal PathologyBeyound Routine Diagnosis, 20 and 21 of May 2016
Abstracts in Conference Proceedings
Meeting of the Portuguese Society of Animal Pathology Beyound Routine Diagnosis, 20 and 21 of May 2016
2
Abstracts in Conference Proceedings
President of Honour
Dr. Henrique Veiga Fernandes
Scientific Committee
Maria da Conceição da Cunha e
Maria dos Anjos Clemente Pires, UTAD.
Helena Vala Correia, ESAV-IPV.
Pedro Faísca, FMV-ULHT
Tânia Carvalho, IMM - FM
Hugo Pissarra, FMV - UTAD
Organizing Committee
Pedro Faísca, FMV-ULHT
Tânia Carvalho, IMM
Andreia Pinto, IMM – FML
Hugo Pissarra, FMV – UL
Fernanda Seixas, UTAD
Helena Vala ESAV-IPV
Fernando Esteves ESAV-IPV
Secretary
Associação de estudantes da FMV
XXI Meeting of the Portuguese Society of Animal PathologyBeyound Routine Diagnosis, 20 and 21 of May 2016
Dr. Henrique Veiga Fernandes
Maria da Conceição da Cunha e Vasconcelos Peleteiro, FMV-UL.
Maria dos Anjos Clemente Pires, UTAD.
IPV.
UTAD
IPV
Associação de estudantes da FMV-ULHT
Meeting of the Portuguese Society of Animal Pathology Beyound Routine Diagnosis, 20 and 21 of May 2016
3
Table of Contents
Editorial
Program
Plenary sessions
Oral communications
Poster presentations
XXI Meeting of the Portuguese Society of Animal PathologyBeyound Routine Diagnosis, 20 and 21 of May 2016
Meeting of the Portuguese Society of Animal Pathology Beyound Routine Diagnosis, 20 and 21 of May 2016
4
5
7
11
17
34
Editorial
A Sociedade Portuguesa de Patologia Animal vai realizar este ano o seu XXI encontro
científico, a 20 e 21 de Maio, que será acolhido pelo Instituto de Medicina Molecular na
Faculdade de Medicina da Universidade de Lisboa.
O tema deste encontro está centrado nas funções de um patologista para além do
diagnóstico de rotina. Convidámos oradores portugueses de renome internacional
nos falar da sua área de a
patologia toxicológica, microscopia ele
Organizamos um workshop de microscopia ele
técnicos e investigadores que queiram ter uma primeira abordagem ou para aprofundar
conhecimentos numa técnica que reganhou preponderância científica nos últimos anos
Neste encontro haverá a oportunidade de partilhar e divulgar experiências em patologia
animal, discutir casos, assim como conhecer os mais recentes avanços científicos dado
nesta área em Portugal.
Será também o momento para entregar o Prémio SPPA para a melhor dissertação de
mestrado defendida em 2015
Aproveite este Encontro para rever colegas e amigos e visitar a cidade de Lisboa na
Primavera.
Poderá encontrar toda a info
XXI Meeting of the Portuguese Society of Animal PathologyBeyound Routine Diagnosis, 20 and 21 of May 2016
A Sociedade Portuguesa de Patologia Animal vai realizar este ano o seu XXI encontro
científico, a 20 e 21 de Maio, que será acolhido pelo Instituto de Medicina Molecular na
da Universidade de Lisboa.
O tema deste encontro está centrado nas funções de um patologista para além do
diagnóstico de rotina. Convidámos oradores portugueses de renome internacional
nos falar da sua área de atuação em áreas tão diversas como patologia experimental,
a toxicológica, microscopia eletrónica, imunocitologia, etc.
um workshop de microscopia eletrónica de transmissão para patologistas,
técnicos e investigadores que queiram ter uma primeira abordagem ou para aprofundar
conhecimentos numa técnica que reganhou preponderância científica nos últimos anos
Neste encontro haverá a oportunidade de partilhar e divulgar experiências em patologia
animal, discutir casos, assim como conhecer os mais recentes avanços científicos dado
Será também o momento para entregar o Prémio SPPA para a melhor dissertação de
mestrado defendida em 2015.
Aproveite este Encontro para rever colegas e amigos e visitar a cidade de Lisboa na
Poderá encontrar toda a informação na página da SPPA www.sppatologianimal.com
Meeting of the Portuguese Society of Animal Pathology Beyound Routine Diagnosis, 20 and 21 of May 2016
5
A Sociedade Portuguesa de Patologia Animal vai realizar este ano o seu XXI encontro
científico, a 20 e 21 de Maio, que será acolhido pelo Instituto de Medicina Molecular na
O tema deste encontro está centrado nas funções de um patologista para além do
diagnóstico de rotina. Convidámos oradores portugueses de renome internacional para
ogia experimental,
trónica de transmissão para patologistas,
técnicos e investigadores que queiram ter uma primeira abordagem ou para aprofundar
conhecimentos numa técnica que reganhou preponderância científica nos últimos anos.
Neste encontro haverá a oportunidade de partilhar e divulgar experiências em patologia
animal, discutir casos, assim como conhecer os mais recentes avanços científicos dados
Será também o momento para entregar o Prémio SPPA para a melhor dissertação de
Aproveite este Encontro para rever colegas e amigos e visitar a cidade de Lisboa na
www.sppatologianimal.com
Editorial
The Portuguese Society of Animal Patology will celebrate its XXI Meeting in the
beautiful city of Lisbon at 20 and 21st of May, at the Instituto d
Faculty of Medicine, University of Lisbon
The theme of this meeting is focused on the duties of a pathologist beyond routine
diagnosis. We invited internationally renowned Portuguese speakers to give talks on
their areas of expertise, such as experimental pathology, toxicological pathology,
electron microscopy, immunocytology, etc.
We organized a workshop of transmission electron microscopy for pathologists,
technicians and researchers who want to have a first approach or to
knowledge in a technique that regained scientific predominance in recent years
At this meeting there will be the opportunity to share your experience and knowledge in
animal pathology, discuss cases and learn about the latest scientific advan
in Portugal.
These will be also the opportunity to deliver the SPPA Award for best master's
dissertation in 2015.
Take advantage of this meeting to review colleagues and friends and visit the city of
Lisbon during spring time.
All information is available in
Board of Directors SPPA
Pedro Faísca
Tânia Carvalho
Hugo Pissarra
Fernanda Seixas
XXI Meeting of the Portuguese Society of Animal PathologyBeyound Routine Diagnosis, 20 and 21 of May 2016
The Portuguese Society of Animal Patology will celebrate its XXI Meeting in the
beautiful city of Lisbon at 20 and 21st of May, at the Instituto de Medicina Molecular,
Faculty of Medicine, University of Lisbon.
The theme of this meeting is focused on the duties of a pathologist beyond routine
diagnosis. We invited internationally renowned Portuguese speakers to give talks on
expertise, such as experimental pathology, toxicological pathology,
electron microscopy, immunocytology, etc.
We organized a workshop of transmission electron microscopy for pathologists,
technicians and researchers who want to have a first approach or to
knowledge in a technique that regained scientific predominance in recent years
At this meeting there will be the opportunity to share your experience and knowledge in
animal pathology, discuss cases and learn about the latest scientific advan
These will be also the opportunity to deliver the SPPA Award for best master's
Take advantage of this meeting to review colleagues and friends and visit the city of
ion is available inwww.sppatologianimal.com
Meeting of the Portuguese Society of Animal Pathology Beyound Routine Diagnosis, 20 and 21 of May 2016
6
The Portuguese Society of Animal Patology will celebrate its XXI Meeting in the
e Medicina Molecular,
The theme of this meeting is focused on the duties of a pathologist beyond routine
diagnosis. We invited internationally renowned Portuguese speakers to give talks on
expertise, such as experimental pathology, toxicological pathology,
We organized a workshop of transmission electron microscopy for pathologists,
technicians and researchers who want to have a first approach or to deepen their
knowledge in a technique that regained scientific predominance in recent years.
At this meeting there will be the opportunity to share your experience and knowledge in
animal pathology, discuss cases and learn about the latest scientific advances in this area
These will be also the opportunity to deliver the SPPA Award for best master's
Take advantage of this meeting to review colleagues and friends and visit the city of
XXI Meeting of the Portuguese Society of Animal PathologyBeyound Routine Diagnosis, 20 and 21 of May 2016
Program
Meeting of the Portuguese Society of Animal Pathology Beyound Routine Diagnosis, 20 and 21 of May 2016
7
XXI Encontro da Sociedade Portuguesa de Patologia
Animal. Patologia Veterinária: Para além do diagnóstico
XXI Meeting of the Portuguese Society of Animal
Pathology. Veterinary Pathology: Beyond routine
20 e 21 de Maio de 2016
20th and 21st May 2016. IMM FM/UL
Hora
Hour
9.00h
9.30h
Presidente de Honra
10.00h
1st plenary session- Paula Oliveira
11.00h
11.30h
2nd plenary session- Afonso Costa
12.30h
13.30h
14.00h
3th plenary session
15.00h
4th plenary session-Nazaré Cunha
16.00h
16.30h
5th plenary session-António Cidadão
17.30h Moderator: Pedro Faísca e Fernando Ferreira
Carla Marrinhas- Diagnóstico citológico em efusões de cães e gatos
o laboratório para um melhor diagnóstico
18.10h
XXI Meeting of the Portuguese Society of Animal PathologyBeyound Routine Diagnosis, 20 and 21 of May 2016
Encontro da Sociedade Portuguesa de Patologia
. Patologia Veterinária: Para além do diagnóstico
de rotina
Meeting of the Portuguese Society of Animal
Veterinary Pathology: Beyond routine
diagnosis
6. Instituto de Medicina Molecular – FM/UL
May 2016. IMM FM/UL
20th May
Registration opens
Opening Session – SPPA direction
Presidente de Honra- Henrique Veiga Fernandes
Moderator: Hugo Pissarra
Paula Oliveira- Murine models of human and animal diseases
Moderator: Fernanda Seixas
Poster Section Tour (P1-P5)
Moderator: Ricardo Marcos
Afonso Costa – Toxicologic Pathology – The role of the pathologist in the
pharmaceutical industry
Lunch time
Moderador: Conceição Peleteiro
Poster Section Tour (P6-P10)
Moderator: Helena Vala
3th plenary session- Afonso Costa- Road do become an ECVP diplomate
Moderador: Maria dos Anjos Pires
Nazaré Cunha- Immunocytochemistry in diagnostic cytology
Coffee Break
Moderador: Irina Amorim
Poster Section Tour (P11-P15)
Moderador: Tânia Carvalho
António Cidadão- Electron microscopy: a good solution or just "high
resolution"?
Moderator: Pedro Faísca e Fernando Ferreira
SPPA Award Ceremony
Diagnóstico citológico em efusões de cães e gatos — aproximando a clínica e
o laboratório para um melhor diagnóstico
SPPA General Assembly
Meeting of the Portuguese Society of Animal Pathology Beyound Routine Diagnosis, 20 and 21 of May 2016
8
Encontro da Sociedade Portuguesa de Patologia
. Patologia Veterinária: Para além do diagnóstico
Meeting of the Portuguese Society of Animal
Veterinary Pathology: Beyond routine
FM/UL.
Sala
Room
Anfit. 57
Piso 01
Anfit. 57
Piso 01
Murine models of human and animal diseases
Anfit. 57
Piso 01
The role of the pathologist in the Anfit. 57
Piso 01
Anfit. 57
Piso 01
Road do become an ECVP diplomate
Anfit. 57
Piso 01
Immunocytochemistry in diagnostic cytology
Anfit. 57
Piso 01
Anfit. 57
Piso 01
Electron microscopy: a good solution or just "high-
Anfit. 57
Piso 01
aproximando a clínica e
Anfit. 57
Piso 01
Anfit. 57
Piso 01
21stMay
Hora
Hour
9:00 Moderator: Sandra Branco
Free communications
9:00 Characterization of histopathological
lesions in a biological model for
environmental studies: Eisenia andrei
Bouché. Preliminar results- Helena Vala
9:15-Routine histological technique applied
to a biological model for environmental
studies- Helena Vala
9:30- Topoisomerase IIα expression in
canine diffuse large B-cell lymphoma-
Pedro Feliciano
9:45-Spontaneous mammary tumor in a
7-week-old female rat- Ana Faustino
10:00- Pathological study of two cases of
mortality in Iberian-lynx (Lynx pardinus)
re-introduced in Portugal in the years
2015-16- Pâmela Valente
10:15-Liver and spleen infiltration with
round cells in a cat- Maria da Conceição
Peleteiro
10.30 Coffee break
11.00 Moderator: Rui Gil da Costa
Free communications
11:00- Fine-needle aspirate cytology of a
mass posterior to the eye- Eva Lemos
11:15- Multiple myeloma: atypical
presentation with cutaneous metastases
- Ricardo Marcos
11:30- A case of T-cell-rich B-cell
lymphoma in a dog- Maria dos Anjos
11:45- Case report: Meduloblastoma in a
three year old dog– Pedro Ruivo
12:00- Establishment of antibody panels
and histochemical techniques in routine
tumour diagnosis in Veterinary
Pathology- Maria dos Anjos Pires
12.15
5th plenary session-Tânia Carvalho
12.30
XXI Meeting of the Portuguese Society of Animal PathologyBeyound Routine Diagnosis, 20 and 21 of May 2016
Technical workshop
Sala
Room
Hora
Hour
9:00 Characterization of histopathological
environmental studies: Eisenia andrei
Helena Vala
Routine histological technique applied
to a biological model for environmental
Topoisomerase IIα expression in
Spontaneous mammary tumor in a
Ana Faustino
Pathological study of two cases of
lynx (Lynx pardinus)
introduced in Portugal in the years
Liver and spleen infiltration with
Maria da Conceição
Anfit. 57
Piso 01
9.00h
Electron Microcopy Workshop
Andreia Pinto
Anfit. 57
Piso 01
Coffee break
needle aspirate cytology of a
Eva Lemos
presentation with cutaneous metastases
Maria dos Anjos
Case report: Meduloblastoma in a
Establishment of antibody panels
and histochemical techniques in routine
Anfit. 57
Piso 01
11.00h
Electron Microcopy Workshop
Andreia Pinto
Moderator: Pedro Faísca
Tânia Carvalho- Electron Microscopy- Cells, Organelles, in diagnosis and
research
Closure session
Meeting of the Portuguese Society of Animal Pathology Beyound Routine Diagnosis, 20 and 21 of May 2016
9
echnical workshop
Sala
Room
Electron Microcopy Workshop-
Histology
Lab
P2A-21
Anfit. 57
Piso 01
Electron Microcopy Workshop-
Andreia Pinto
Histology
Lab
P2A-21
Cells, Organelles, in diagnosis and Anfit. 57
Piso 01
Anfit. 57
Piso 01
1-Cyclooxygenase-2 expression during urinary bladder carcinogenesis in ICR mice
2-Ptaquiloside from bracken (Pteridium spp.) inhibits tumour
Gil da Costa
3-Extraskeletal sarcomas: Retrospective study of 5 years (2011
4-OvisOme: an updated database for Ovis aries
5- A case of Myxosarcoma in a Chilean Flamingo (Phoenicopterus chilensis) of a Zoologycal Collection "Quinta dos
Plátanos- Vera Pessoa
6-Stromal characterization of feline endometrial adenocarcinomas (FEA)
7-Morphological an immunohistochemical characterization of the cat ovary
8-Comparative study of immunohistochemical expression of TGFβ1 in feline and canine mammary lesions
Fernanda Seixas
9-Keratin 8 and 18 expressionin normal uterus, cystic endometrial hyperplasia (CEH) and feline endometrial
adenocarcinoma (FEA) - Maria dos Anjos Pires
10-In vitro and in vivo model for the study of P
Figueira
11- Chrysosporium anamorph of Nannizziopsis vriesii (CANV) lesions in bearded dragon (Pogona vitticeps)
Fernanda Seixas
12-The histopathological timeframe of Hyalomma lusitanicum infestation development on bovi
13-Identification of subcutaneous mite Hypodectes propus (Acarina: Hypoderatidae) in the Northern gannet
(Morus bassanus) in Portugal- Fernanda Seixas
14-Dirofilaria immitis in South African fur seal (Arctocephalus pusillus pusillus): necropsy and histopathology
findings observed in zoological context in Portugal
15-Occurrence of blood-sucking mites in ornamental chickens
XXI Meeting of the Portuguese Society of Animal PathologyBeyound Routine Diagnosis, 20 and 21 of May 2016
Poster Section Tour (P1-P5)
2 expression during urinary bladder carcinogenesis in ICR mice- Regina Arantes
Ptaquiloside from bracken (Pteridium spp.) inhibits tumour-infiltrating CD8+ T cells in HPV-transgenic mice
Extraskeletal sarcomas: Retrospective study of 5 years (2011-2016)- Leonor Delgado
OvisOme: an updated database for Ovis aries- Helena Vala
A case of Myxosarcoma in a Chilean Flamingo (Phoenicopterus chilensis) of a Zoologycal Collection "Quinta dos
Poster Section Tour (P6-P10)
Stromal characterization of feline endometrial adenocarcinomas (FEA) - Maria dos Anjos Pires
Morphological an immunohistochemical characterization of the cat ovary -Maria dos Anjos Pires
Comparative study of immunohistochemical expression of TGFβ1 in feline and canine mammary lesions
Keratin 8 and 18 expressionin normal uterus, cystic endometrial hyperplasia (CEH) and feline endometrial
Maria dos Anjos Pires
In vitro and in vivo model for the study of P-cadherin in feline mammary carcinogenesis - Ana Catari
Poster Section Tour (P11-P15)
Chrysosporium anamorph of Nannizziopsis vriesii (CANV) lesions in bearded dragon (Pogona vitticeps)
The histopathological timeframe of Hyalomma lusitanicum infestation development on bovi
Identification of subcutaneous mite Hypodectes propus (Acarina: Hypoderatidae) in the Northern gannet
Fernanda Seixas
Dirofilaria immitis in South African fur seal (Arctocephalus pusillus pusillus): necropsy and histopathology
findings observed in zoological context in Portugal- Inês Marcelino
sucking mites in ornamental chickens- Ana Maria Araújo
Meeting of the Portuguese Society of Animal Pathology Beyound Routine Diagnosis, 20 and 21 of May 2016
10
Regina Arantes
transgenic mice- Rui
A case of Myxosarcoma in a Chilean Flamingo (Phoenicopterus chilensis) of a Zoologycal Collection "Quinta dos
res
Maria dos Anjos Pires
Comparative study of immunohistochemical expression of TGFβ1 in feline and canine mammary lesions –
Keratin 8 and 18 expressionin normal uterus, cystic endometrial hyperplasia (CEH) and feline endometrial
Ana Catarina
Chrysosporium anamorph of Nannizziopsis vriesii (CANV) lesions in bearded dragon (Pogona vitticeps) -
The histopathological timeframe of Hyalomma lusitanicum infestation development on bovines -Sara Zúquete
Identification of subcutaneous mite Hypodectes propus (Acarina: Hypoderatidae) in the Northern gannet
Dirofilaria immitis in South African fur seal (Arctocephalus pusillus pusillus): necropsy and histopathology
Plenary Session
XXI Meeting of the Portuguese Society of Animal PathologyBeyound Routine Diagnosis, 20 and 21 of May 2016
Plenary Session
Meeting of the Portuguese Society of Animal Pathology Beyound Routine Diagnosis, 20 and 21 of May 2016
11
Murine models of human and animal diseases
Paula A. Oliveira
Department of Veterinary Sciences, CITAB
Technology of Agro-Environmental and Biological Sciences, University of Trás
Montes and Alto Douro, Vila Real
In this communication,
rodents (rats and mice) to study cancer, namely urinary bladder, liver, mammary and
skin cancer. These models have been used for either
underlying cancer development or improving therapy. Although the widespread use of
in vitro systems that permit repeating almost all the processes involved in the
development of a disease, only the use of murine models en
the mechanisms of human and animal diseases. Thereby providing the only system
known today to evaluate new drugs or lifestyles for therapeutic intervention. A better
understanding of cancer murine models and their uses as well as
aid future researchers regarding the development and implementation of new targeted
therapies and chemotherapeutic agents for human and animal cancer.
XXI Meeting of the Portuguese Society of Animal PathologyBeyound Routine Diagnosis, 20 and 21 of May 2016
Murine models of human and animal diseases
Department of Veterinary Sciences, CITAB - Centre for the Research and
Environmental and Biological Sciences, University of Trás
and Alto Douro, Vila Real, Portugal
I will address original research performed with laboratory
rodents (rats and mice) to study cancer, namely urinary bladder, liver, mammary and
skin cancer. These models have been used for either understanding the molecular events
underlying cancer development or improving therapy. Although the widespread use of
in vitro systems that permit repeating almost all the processes involved in the
development of a disease, only the use of murine models enable us to reproduce onset
the mechanisms of human and animal diseases. Thereby providing the only system
known today to evaluate new drugs or lifestyles for therapeutic intervention. A better
understanding of cancer murine models and their uses as well as their limitations may
s regarding the development and implementation of new targeted
therapies and chemotherapeutic agents for human and animal cancer.
Meeting of the Portuguese Society of Animal Pathology Beyound Routine Diagnosis, 20 and 21 of May 2016
12
Centre for the Research and
Environmental and Biological Sciences, University of Trás-os-
original research performed with laboratory
rodents (rats and mice) to study cancer, namely urinary bladder, liver, mammary and
understanding the molecular events
underlying cancer development or improving therapy. Although the widespread use of
in vitro systems that permit repeating almost all the processes involved in the
able us to reproduce onset
the mechanisms of human and animal diseases. Thereby providing the only system
known today to evaluate new drugs or lifestyles for therapeutic intervention. A better
their limitations may
s regarding the development and implementation of new targeted
Toxicologic Pathology industry
Afonso Costa laboratório
Non clinical Drug Safety da Boehringer Ingelheim Pharma GmbH & Co.
The discovery and development of new drug compounds is a very long, complex,
highly regulated, expensive and risky process. It is estimated that only 1 out
drug candidates reaches market authorization.
can rise to an estimated US $ 1.2 billion.
candidates include toxicity, tolerance, and lack of efficacy or bioavailab
Earlyprediction of safety liabilities can lead to a better selection of drug candidatesand
prevent failures in latter stages of pre
of liabilities such as hepatotoxicity before trials
development costs per drug, making the development of predictive technologies and
new toxicity testing strategies a major priority of pharmaceutical companies.The
toxicologic pathologist plays a pivotal role in drug safet
assessments. At all stages from discovery and development, the toxicological
pathologist with the combination of clinical and scientific training can contributeto a
better understanding of the pathophysiology and mechanisms of adverse
eventsand in particular to the assessment of their relevance to humans.
References:
1- Kerlin L.R., Li X. Pathology in non
Haschek and Rousseaux´s handbook of toxicologic pathology. Haschek W.M,
Rousseaux C.G., Wa
2- Kramer J.A.,Sagartz J.E., Morris D.L.The application of discovery
toxicology and pathology towards the design of safer pharmaceutical lead
candidates. Nature Reviews Drug Discovery,2007:
XXI Meeting of the Portuguese Society of Animal PathologyBeyound Routine Diagnosis, 20 and 21 of May 2016
Toxicologic Pathology – The role of the pathologist in the pharmaceutical
laboratório
Non clinical Drug Safety da Boehringer Ingelheim Pharma GmbH & Co.
The discovery and development of new drug compounds is a very long, complex,
highly regulated, expensive and risky process. It is estimated that only 1 out
drug candidates reaches market authorization.1 The costs of developing a single drug
can rise to an estimated US $ 1.2 billion.1 Frequent reasons for discontinuation of drug
candidates include toxicity, tolerance, and lack of efficacy or bioavailab
Earlyprediction of safety liabilities can lead to a better selection of drug candidatesand
prevent failures in latter stages of pre-clinical or clinical development.2
of liabilities such as hepatotoxicity before trials could save up to US $ 2 billion in
development costs per drug, making the development of predictive technologies and
new toxicity testing strategies a major priority of pharmaceutical companies.The
toxicologic pathologist plays a pivotal role in drug safety and risk management
assessments. At all stages from discovery and development, the toxicological
pathologist with the combination of clinical and scientific training can contributeto a
better understanding of the pathophysiology and mechanisms of adverse
eventsand in particular to the assessment of their relevance to humans.2
Kerlin L.R., Li X. Pathology in non-clinical drug safety assessment. In:
Haschek and Rousseaux´s handbook of toxicologic pathology. Haschek W.M,
Rousseaux C.G., Wallig M.A. (eds), 2007: 3rd edition: 725-732
Kramer J.A.,Sagartz J.E., Morris D.L.The application of discovery
toxicology and pathology towards the design of safer pharmaceutical lead
candidates. Nature Reviews Drug Discovery,2007: 6, 636-649
Meeting of the Portuguese Society of Animal Pathology Beyound Routine Diagnosis, 20 and 21 of May 2016
13
The role of the pathologist in the pharmaceutical
Non clinical Drug Safety da Boehringer Ingelheim Pharma GmbH & Co. KG
The discovery and development of new drug compounds is a very long, complex,
highly regulated, expensive and risky process. It is estimated that only 1 out of 1000
The costs of developing a single drug
Frequent reasons for discontinuation of drug
candidates include toxicity, tolerance, and lack of efficacy or bioavailability in humans.2
Earlyprediction of safety liabilities can lead to a better selection of drug candidatesand 2Early prediction
could save up to US $ 2 billion in
development costs per drug, making the development of predictive technologies and
new toxicity testing strategies a major priority of pharmaceutical companies.The
y and risk management
assessments. At all stages from discovery and development, the toxicological
pathologist with the combination of clinical and scientific training can contributeto a
better understanding of the pathophysiology and mechanisms of adverse toxic
clinical drug safety assessment. In:
Haschek and Rousseaux´s handbook of toxicologic pathology. Haschek W.M,
Kramer J.A.,Sagartz J.E., Morris D.L.The application of discovery
toxicology and pathology towards the design of safer pharmaceutical lead
Immunocytochemistry in diagnostic cytology
Nazaré Pinto da Cunha
CEDIVET, Porto, Portugal; FMV
Immunocytochemistry (ICC) is a technique for the detection of cellular antigens in
cytologic specimens, using specific antibodiesbound
visualized using a light microscope. The use of ICC is a powerful adjunct to cytological
diagnosis, contributing to an increase in diagnostic accurary.
While in human medicine is already part of the routine work in most cy
laboratories, in veterinary medicine ICC has only recently gained a recognized value. In
2016 was published the first consensus statement
future directions of immunocytochemistry in veterinary medicine.
The major application of ICC in cytology is the classification of neoplasia, but it
can also be used to determine prognosis, identification of etiologic agents and as a
marker of cell growth and differentiation. Major advantages over other methods are
greater antigen preservation, simultaneous assessment of immunostaining and cell
morphology,and minimally invasive sampling.
ICC can be done with different types of specimens, more often fine needle
aspirates but also with various types of exfoliated cells, such as e
respiratory cytology. Samples may be processed in several ways: smears, cytospins, cell
block,monolayered liquid-
processing of the specimens that is determinant for the ICC results.
can be performed in non-stained acetone of formalin
stained slides.3,4
There are several antibodies,with different sensitivities and specificities,
available for ICC on animal tissues.Ideally, a panel (2 or mor
selected and results should always be interpreted together with the routine cytologic
staining. A brief list of frequently used antibodies on veterinary ICC
includes:cytokeratin (CK) AE1/AE3, CK7, CK14, CK18, CK20 and EMA for epitel
neoplasia; CD1, CD11, CD18, CD90, CD204, E
neoplasia; CD3, CD4, CD8, CD20, CD21, CD45, CD79a, PAX
neoplasia; Melan-A for melanocytic neoplasia; S
CD31 andmyoglobolin for mesenchymal neoplasia.
XXI Meeting of the Portuguese Society of Animal PathologyBeyound Routine Diagnosis, 20 and 21 of May 2016
Immunocytochemistry in diagnostic cytology
CEDIVET, Porto, Portugal; FMV - ULHT, Lisboa, Portugal
Immunocytochemistry (ICC) is a technique for the detection of cellular antigens in
cytologic specimens, using specific antibodiesbound to a chromogen, which are then
visualized using a light microscope. The use of ICC is a powerful adjunct to cytological
diagnosis, contributing to an increase in diagnostic accurary.1
While in human medicine is already part of the routine work in most cy
laboratories, in veterinary medicine ICC has only recently gained a recognized value. In
2016 was published the first consensus statement regarding the use, publication and
future directions of immunocytochemistry in veterinary medicine.2
application of ICC in cytology is the classification of neoplasia, but it
can also be used to determine prognosis, identification of etiologic agents and as a
marker of cell growth and differentiation. Major advantages over other methods are
en preservation, simultaneous assessment of immunostaining and cell
morphology,and minimally invasive sampling.2
ICC can be done with different types of specimens, more often fine needle
aspirates but also with various types of exfoliated cells, such as e
respiratory cytology. Samples may be processed in several ways: smears, cytospins, cell
-based (LBC) slides, but it is the proper handling and
processing of the specimens that is determinant for the ICC results.2,3
stained acetone of formalin-fixed smears, but also in already
There are several antibodies,with different sensitivities and specificities,
available for ICC on animal tissues.Ideally, a panel (2 or more) of markers should be
selected and results should always be interpreted together with the routine cytologic
staining. A brief list of frequently used antibodies on veterinary ICC
cytokeratin (CK) AE1/AE3, CK7, CK14, CK18, CK20 and EMA for epitel
neoplasia; CD1, CD11, CD18, CD90, CD204, E-cadherinand MHCII for histiocytic
neoplasia; CD3, CD4, CD8, CD20, CD21, CD45, CD79a, PAX-5 for lymphoid
A for melanocytic neoplasia; S-100, vimentin, desmin, factor VIII,
or mesenchymal neoplasia.2
Meeting of the Portuguese Society of Animal Pathology Beyound Routine Diagnosis, 20 and 21 of May 2016
14
Immunocytochemistry (ICC) is a technique for the detection of cellular antigens in
to a chromogen, which are then
visualized using a light microscope. The use of ICC is a powerful adjunct to cytological
While in human medicine is already part of the routine work in most cytology
laboratories, in veterinary medicine ICC has only recently gained a recognized value. In
regarding the use, publication and
application of ICC in cytology is the classification of neoplasia, but it
can also be used to determine prognosis, identification of etiologic agents and as a
marker of cell growth and differentiation. Major advantages over other methods are
en preservation, simultaneous assessment of immunostaining and cell
ICC can be done with different types of specimens, more often fine needle
aspirates but also with various types of exfoliated cells, such as effusions and
respiratory cytology. Samples may be processed in several ways: smears, cytospins, cell
based (LBC) slides, but it is the proper handling and 2,3 This technique
fixed smears, but also in already
There are several antibodies,with different sensitivities and specificities,
e) of markers should be
selected and results should always be interpreted together with the routine cytologic
staining. A brief list of frequently used antibodies on veterinary ICC
cytokeratin (CK) AE1/AE3, CK7, CK14, CK18, CK20 and EMA for epitelial
cadherinand MHCII for histiocytic
5 for lymphoid
100, vimentin, desmin, factor VIII,
1. Skoog L.,Tani E
routine cytology.Cytopathology.
2. Priest H.L., Hume K
Snyder L.A., Springer N
future directions of immunocytochemistry in veterinary medicine: a consensus
of the Oncology-Pathology Working Group. Vet Comp Oncol. 2016 Mar 22.
[Epub ahead of print]
3. Ramos-Vara J
along: revisiting the technical aspects of immunohistochemistry
and blue technique. Vet Pathol. 2014 Jan;51(1):42
4. Stone B.M., Gan D. Application of the tissue transfer technique in
veterinary cytopathology. Vet ClinPat
XXI Meeting of the Portuguese Society of Animal PathologyBeyound Routine Diagnosis, 20 and 21 of May 2016
,Tani E. Immunocytochemistry: an indispensable technique in
Cytopathology. 2011;22(4):215-29.
, Hume K.R., Killick D., Kozicki A., Rizzo V
, Springer N.L., Wright Z.M., Robat C. The use, publication and
future directions of immunocytochemistry in veterinary medicine: a consensus
Pathology Working Group. Vet Comp Oncol. 2016 Mar 22.
[Epub ahead of print]
Vara J.A., Miller M.A. When tissue antigens and antibodies get
along: revisiting the technical aspects of immunohistochemistry
and blue technique. Vet Pathol. 2014 Jan;51(1):42-87
, Gan D. Application of the tissue transfer technique in
cytopathology. Vet ClinPathol. 2014 Jun;43(2):295-302
Meeting of the Portuguese Society of Animal Pathology Beyound Routine Diagnosis, 20 and 21 of May 2016
15
Immunocytochemistry: an indispensable technique in
, Rizzo V.L., Seelig D.,
, Robat C. The use, publication and
future directions of immunocytochemistry in veterinary medicine: a consensus
Pathology Working Group. Vet Comp Oncol. 2016 Mar 22.
and antibodies get
along: revisiting the technical aspects of immunohistochemistry-the red, brown,
, Gan D. Application of the tissue transfer technique in
302
Oral communications
XXI Meeting of the Portuguese Society of Animal PathologyBeyound Routine Diagnosis, 20 and 21 of May 2016
Oral communications
Meeting of the Portuguese Society of Animal Pathology Beyound Routine Diagnosis, 20 and 21 of May 2016
16
1. Characterization of histopathological lesions in a biological model for
environmental studies: Eisenia andrei Bouché
Vala H.1,2*, Rodriguez-Seijo A
Costa J.5, Pereira R.6
1Agrarian School of Viseu, Polytechnic Institute of Viseu. Viseu, Portugal2Centre for the Research and Technology of Agro
Sciences (CITAB and Centre for Studies in
(CI&DETS), Portugal 3Department of Plant Biology and Soil Science, Universidade de Vigo, As Lagoas,
Marcosende, 36310 Vigo, Spain.4Department of Biology & CESAM, University of Aveiro, Aveiro, Portugal5Department of Chemistry & CESAM, University of Aveiro, Aveiro, Portugal6Department of Biology, Faculty of Sciences of the University of Porto & CIIMAR
Interdisciplinary Centre of Marine and Environmental Research & GreenUP/CITAB
UP, Porto, Portugal
* hvala2@gmail.com
Introduction: The strong expansion of the world production of plastics caused a
severe accumulation of plastic debris in the environment, which makes them one of the
most important contaminants, growing as a global e
production in Europe has been relatively constant in the last 10 years, world plastic
production continues to increase, affecting soil biota and their functions.
Objectives:Thus, in order to evaluate the effects of MP in
earthworms (Eisenia andrei Bouché
with MPs and the authors documented, using microscopic figures, the pathological
lesions found in this biological model.
Material and Methods:
with different concentrations of MP (mg/kgdw) with sizes ranging
mm, were fixed in 10% neutral
histopathological diagnosis.
XXI Meeting of the Portuguese Society of Animal PathologyBeyound Routine Diagnosis, 20 and 21 of May 2016
1. Characterization of histopathological lesions in a biological model for
Eisenia andrei Bouché. Preliminar results.
Seijo A.3, Lourenço J.4, Francisco A.1, Rocha
Agrarian School of Viseu, Polytechnic Institute of Viseu. Viseu, Portugal
Centre for the Research and Technology of Agro-Environmental and Biological
Sciences (CITAB and Centre for Studies in Education, and Health Technologies
Department of Plant Biology and Soil Science, Universidade de Vigo, As Lagoas,
Marcosende, 36310 Vigo, Spain.
ment of Biology & CESAM, University of Aveiro, Aveiro, Portugal
istry & CESAM, University of Aveiro, Aveiro, Portugal
Department of Biology, Faculty of Sciences of the University of Porto & CIIMAR
Interdisciplinary Centre of Marine and Environmental Research & GreenUP/CITAB
The strong expansion of the world production of plastics caused a
severe accumulation of plastic debris in the environment, which makes them one of the
most important contaminants, growing as a global environmental problem. Although the
production in Europe has been relatively constant in the last 10 years, world plastic
production continues to increase, affecting soil biota and their functions.
Thus, in order to evaluate the effects of MP in soil-dwelling organisms,
Eisenia andrei Bouché), were exposed to standard artificial soil mixed
with MPs and the authors documented, using microscopic figures, the pathological
lesions found in this biological model.
Material and Methods:Eight adult earthworms extracted from soils contaminated
with different concentrations of MP (mg/kgdw) with sizes ranging between 250
, were fixed in 10% neutral-buffered formalin and processed for routine
histopathological diagnosis.
Meeting of the Portuguese Society of Animal Pathology Beyound Routine Diagnosis, 20 and 21 of May 2016
17
1. Characterization of histopathological lesions in a biological model for
, Rocha-Santos T.5,
Agrarian School of Viseu, Polytechnic Institute of Viseu. Viseu, Portugal
Environmental and Biological
Education, and Health Technologies
Department of Plant Biology and Soil Science, Universidade de Vigo, As Lagoas,
ment of Biology & CESAM, University of Aveiro, Aveiro, Portugal
istry & CESAM, University of Aveiro, Aveiro, Portugal
Department of Biology, Faculty of Sciences of the University of Porto & CIIMAR -
Interdisciplinary Centre of Marine and Environmental Research & GreenUP/CITAB-
The strong expansion of the world production of plastics caused a
severe accumulation of plastic debris in the environment, which makes them one of the
nvironmental problem. Although the
production in Europe has been relatively constant in the last 10 years, world plastic
production continues to increase, affecting soil biota and their functions.
dwelling organisms,
), were exposed to standard artificial soil mixed
with MPs and the authors documented, using microscopic figures, the pathological
t adult earthworms extracted from soils contaminated
between 250-1000
buffered formalin and processed for routine
Results and discussion
throughout the GI tube of earthworms but several lesions were found in the individuals
extracted from the soils with high MP concentrations, when compared with control
group, namely epithelial in
this stress agent.
Conclusion: Earthworms have probably avoided the consumption of the biggest
MPs. However, evidences point for lesions that were likely caused by the smallest MPs
that were likely egested during the depuration phase.
Acknowledgments: CITAB; FCT (UID/AGR/04033/2013 e POCI
FEDER-006958). CI&DETS, FCT
XXI Meeting of the Portuguese Society of Animal PathologyBeyound Routine Diagnosis, 20 and 21 of May 2016
ssion: Contrary to what would be expected, MP were not found
throughout the GI tube of earthworms but several lesions were found in the individuals
extracted from the soils with high MP concentrations, when compared with control
group, namely epithelial intestinal atrophy and evidences of inflammatory responses to
Earthworms have probably avoided the consumption of the biggest
MPs. However, evidences point for lesions that were likely caused by the smallest MPs
ely egested during the depuration phase.
CITAB; FCT (UID/AGR/04033/2013 e POCI
CI&DETS, FCT
Meeting of the Portuguese Society of Animal Pathology Beyound Routine Diagnosis, 20 and 21 of May 2016
18
Contrary to what would be expected, MP were not found
throughout the GI tube of earthworms but several lesions were found in the individuals
extracted from the soils with high MP concentrations, when compared with control
testinal atrophy and evidences of inflammatory responses to
Earthworms have probably avoided the consumption of the biggest
MPs. However, evidences point for lesions that were likely caused by the smallest MPs
CITAB; FCT (UID/AGR/04033/2013 e POCI-01-0145-
Routine histological technique applied to a biological model for environmental studies
Vala H.1,2*,Rodriguez-Seijo ASantos T.5, Costa J.5, Pereira R
1 Agrarian School of Viseu, Polytechnic Institute of Viseu. 2 CITAB and CI&DETS, Portugal3 Department of Plant Biology and Soil Science, Universidade de Vigo, As Lagoas,
Marcosende, 36310 Vigo, Spain.4 Department of Biology & CESAM, University of Aveiro, Aveiro, Portugal5 Department of Chemistry & CESAM, University of Aveiro, Aveiro, Portugal6 Department of Biology, Faculty of Sciences of the University of Porto & CIIMAR
Interdisciplinary Centre of Marine and Environmental Research & GreenUP/CITAB
UP, Porto, Portugal
* hvala2@gmail.com
Introduction and aim:
standard ecotoxicological tests (e.g. ISO, 2008, 2012; OECD, 2004, 1094) and they are
also model organisms to test soil structure and permeability, as well as for important
soil services as the degradation organic matter.
Although histopathological changes have been demonstrated to be good biomarkers
to assess the exposure of these animals to different physical and chemical stress agents,
studies with clear and high quality images describi
scarce in the literature, so the aim of this work was to better characterize this biological
model.
Material and Methods:
(OECD, 2004) for 28 days, were extracted
gut content, fixed in 10% neutral
histopathological diagnosis.
Results and discussion
difficulties were faced related with microtome sectioning, resulting in artefacts, namely
XXI Meeting of the Portuguese Society of Animal PathologyBeyound Routine Diagnosis, 20 and 21 of May 2016
Routine histological technique applied to a biological model for environmental
Seijo A.3, Lourenço J.4, Garcia C.1, Francisco A, Pereira R.6
Agrarian School of Viseu, Polytechnic Institute of Viseu. Viseu, Portugal
CITAB and CI&DETS, Portugal
Department of Plant Biology and Soil Science, Universidade de Vigo, As Lagoas,
Marcosende, 36310 Vigo, Spain.
ment of Biology & CESAM, University of Aveiro, Aveiro, Portugal
Department of Chemistry & CESAM, University of Aveiro, Aveiro, Portugal
Department of Biology, Faculty of Sciences of the University of Porto & CIIMAR
Interdisciplinary Centre of Marine and Environmental Research & GreenUP/CITAB
: Earthworms are an important test organisms used in several
standard ecotoxicological tests (e.g. ISO, 2008, 2012; OECD, 2004, 1094) and they are
also model organisms to test soil structure and permeability, as well as for important
as the degradation organic matter.
Although histopathological changes have been demonstrated to be good biomarkers
to assess the exposure of these animals to different physical and chemical stress agents,
studies with clear and high quality images describing normal tissue conditions are
scarce in the literature, so the aim of this work was to better characterize this biological
Material and Methods:Eight adult earthworms exposed to an artificial standard soil
(OECD, 2004) for 28 days, were extracted and placed in a plastic box to depurate their
gut content, fixed in 10% neutral-buffered formalin and processed for routine
histopathological diagnosis.
Results and discussion: Satisfactory histological sections were obtained. Some
difficulties were faced related with microtome sectioning, resulting in artefacts, namely
Meeting of the Portuguese Society of Animal Pathology Beyound Routine Diagnosis, 20 and 21 of May 2016
19
Routine histological technique applied to a biological model for environmental
, Francisco A.1, Rocha-
Viseu, Portugal
Department of Plant Biology and Soil Science, Universidade de Vigo, As Lagoas,
ment of Biology & CESAM, University of Aveiro, Aveiro, Portugal
Department of Chemistry & CESAM, University of Aveiro, Aveiro, Portugal
Department of Biology, Faculty of Sciences of the University of Porto & CIIMAR -
Interdisciplinary Centre of Marine and Environmental Research & GreenUP/CITAB-
Earthworms are an important test organisms used in several
standard ecotoxicological tests (e.g. ISO, 2008, 2012; OECD, 2004, 1094) and they are
also model organisms to test soil structure and permeability, as well as for important
Although histopathological changes have been demonstrated to be good biomarkers
to assess the exposure of these animals to different physical and chemical stress agents,
ng normal tissue conditions are
scarce in the literature, so the aim of this work was to better characterize this biological
Eight adult earthworms exposed to an artificial standard soil
and placed in a plastic box to depurate their
buffered formalin and processed for routine
Satisfactory histological sections were obtained. Some
difficulties were faced related with microtome sectioning, resulting in artefacts, namely
lines across sections produced by a nick in the cutting edge of the microtome knife
cutting tear, motivated by the presence of sand and other solid particles that persisted in
the gut of earthworms. Nevertheless, it was possible to obtain representative figures
from different earthworm sections.
Conclusion: Routine histological technique was effective for obtainin
histological sections and the knowledge of the histology of earthworms could be very
useful for future application in environmental studies, using this biological model.
Acknowledgments: CITAB; FCT (UID/AGR/04033/2013 e POCI
FEDER-006958). CI&DETS, FCT
XXI Meeting of the Portuguese Society of Animal PathologyBeyound Routine Diagnosis, 20 and 21 of May 2016
lines across sections produced by a nick in the cutting edge of the microtome knife
the presence of sand and other solid particles that persisted in
the gut of earthworms. Nevertheless, it was possible to obtain representative figures
from different earthworm sections.
Routine histological technique was effective for obtainin
histological sections and the knowledge of the histology of earthworms could be very
useful for future application in environmental studies, using this biological model.
CITAB; FCT (UID/AGR/04033/2013 e POCI
CI&DETS, FCT
Meeting of the Portuguese Society of Animal Pathology Beyound Routine Diagnosis, 20 and 21 of May 2016
20
lines across sections produced by a nick in the cutting edge of the microtome knife
the presence of sand and other solid particles that persisted in
the gut of earthworms. Nevertheless, it was possible to obtain representative figures
Routine histological technique was effective for obtaining satisfactory
histological sections and the knowledge of the histology of earthworms could be very
useful for future application in environmental studies, using this biological model.
CITAB; FCT (UID/AGR/04033/2013 e POCI-01-0145-
Topoisomerase IIα expression in canine diffuse large B
Feliciano P.*, Ferreira F.,
The Centre for Interdisciplinary Research in Animal Health (CIISA), Faculty of
Veterinary Medicine, University of Lisbon.
* pedro_gfeliciano@hotmail.com
Canine lymphomas are one of the most common neoplasms in dogs. These tumors
represent a clonal expansion of lymphoid cells with distinctive morphological and
immunophenotypical features, with the diffuse large B
common subtype.
Topoisomerase IIα is a multifunctional nuclear protein and it plays an essential role
in cellular proliferation. It is also an important therapeutic target in haematopoie
tumors.
Although several reports showed that expression levels of topoisomerase II
be a predictive factor in human non
lymphoma is still unknown. The aims of this study were to evaluate the expression o
topoisomerase IIα in canine B
The immunohistochemical technique was optimised in order to obtain specific
staining. Several tests were performed using two primary antibodies: Ki
Millipore) and #4733 (Cell Signaling Technologies). The selected antibody was Ki
Thirteen cases of canine diffuse large B
counting was performed in the ones with positive staining. Observed staining was
divided according to an intensity scale. Correlation levels between topoisomerase II
expression, Ki-67 proliferation index and survival analysis were performed. Due to the
small number of analysed cases, no statistically significant results were obtained.
This study allowed the assessment of topoisomerase II
methods in canine lymphomas, using immunohistochemical technique. Although the
reduced number of cases has limited this work, further studies must be performed in
order to assess the predictive
XXI Meeting of the Portuguese Society of Animal PathologyBeyound Routine Diagnosis, 20 and 21 of May 2016
expression in canine diffuse large B-cell lymphoma
Feliciano P.*, Ferreira F., Correia J.
The Centre for Interdisciplinary Research in Animal Health (CIISA), Faculty of
Veterinary Medicine, University of Lisbon.
pedro_gfeliciano@hotmail.com
Canine lymphomas are one of the most common neoplasms in dogs. These tumors
represent a clonal expansion of lymphoid cells with distinctive morphological and
pical features, with the diffuse large B-cell lymphoma being the most
is a multifunctional nuclear protein and it plays an essential role
in cellular proliferation. It is also an important therapeutic target in haematopoie
Although several reports showed that expression levels of topoisomerase II
be a predictive factor in human non-Hodgkin lymphoma, its importance in canine
lymphoma is still unknown. The aims of this study were to evaluate the expression o
in canine B-cell lymphomas and to validate its prognostic value.
The immunohistochemical technique was optimised in order to obtain specific
staining. Several tests were performed using two primary antibodies: Ki
) and #4733 (Cell Signaling Technologies). The selected antibody was Ki
Thirteen cases of canine diffuse large B-cell lymphoma were analysed and cell
counting was performed in the ones with positive staining. Observed staining was
an intensity scale. Correlation levels between topoisomerase II
67 proliferation index and survival analysis were performed. Due to the
small number of analysed cases, no statistically significant results were obtained.
he assessment of topoisomerase IIα staining and quantification
methods in canine lymphomas, using immunohistochemical technique. Although the
reduced number of cases has limited this work, further studies must be performed in
order to assess the predictive value of topoisomerase IIα expression levels.
Meeting of the Portuguese Society of Animal Pathology Beyound Routine Diagnosis, 20 and 21 of May 2016
21
cell lymphoma
The Centre for Interdisciplinary Research in Animal Health (CIISA), Faculty of
Canine lymphomas are one of the most common neoplasms in dogs. These tumors
represent a clonal expansion of lymphoid cells with distinctive morphological and
cell lymphoma being the most
is a multifunctional nuclear protein and it plays an essential role
in cellular proliferation. It is also an important therapeutic target in haematopoietic
Although several reports showed that expression levels of topoisomerase IIα could
Hodgkin lymphoma, its importance in canine
lymphoma is still unknown. The aims of this study were to evaluate the expression of
cell lymphomas and to validate its prognostic value.
The immunohistochemical technique was optimised in order to obtain specific
staining. Several tests were performed using two primary antibodies: Ki-S1 (MAB4197,
) and #4733 (Cell Signaling Technologies). The selected antibody was Ki-S1.
cell lymphoma were analysed and cell
counting was performed in the ones with positive staining. Observed staining was
an intensity scale. Correlation levels between topoisomerase IIα
67 proliferation index and survival analysis were performed. Due to the
small number of analysed cases, no statistically significant results were obtained.
staining and quantification
methods in canine lymphomas, using immunohistochemical technique. Although the
reduced number of cases has limited this work, further studies must be performed in
expression levels.
Spontaneous mammary tumor in a 7
Faustino-Rocha A.I1,2,3,4
1Department of Veterinary Sciences, School of Agrarian and
University of Trás-os-Montes and Alto Douro, UTAD, www.utad.pt, 5001
Real, Portugal 2 Center for the Research and Technology of Agro
Sciences (CITAB), UTAD, www.utad.pt, 50013 Animal and Veterinary Research Center (CECAV), School of Agrarian and
Veterinary Sciences, UTAD, www.utad.pt, 50014 Organic Chemistry of Natural Products and Agrifood (QOPNA), Mass
Spectrometry Center, Department of Chemist
www.geral@ua.pt, 3810-193, Aveiro, Portugal
* anafaustino.faustino@sapo.pt
Introduction and aims:
extremely rare during the first year of life. The present work describes a spontaneous
mammary carcinoma in a 7
Case description: Thirty
were used in an assay of chemically
of quarantine, animals were randomly divided into five experimental groups and were
allowed to acclimate to the lab conditions for two weeks. All procedures followed the
National and European legislation, and were approved by National (Ap
008961) and University (CE_12
No alterations were found during the quarantine or acclimatization period. However,
at fifty days of age, one animal from control group exhibited a movable mass in the left
cervical region. The mass grown quickly and the animal health status deteriorated faster.
Eight days later, the animal was humanely sacrificed. A complete necropsy was
performed. The mass cut surface was reddish in appearance with several cysts filled
with a bloody content. The mass was immersed in buffered formalin and routinely
processed for light microscopy. It was stained with hematoxylin and eosin (H&E),
periodic acid-Schiff (PAS) and immunohistochemically evaluated by staining with an
antibody against estrogen recepto
XXI Meeting of the Portuguese Society of Animal PathologyBeyound Routine Diagnosis, 20 and 21 of May 2016
Spontaneous mammary tumor in a 7-week-old female rat 2,3,4*, Gama A.1,3, Oliveira P.A.1,2, Ferreira R.4, Ginja M
Department of Veterinary Sciences, School of Agrarian and Veterinary Sciences,
Montes and Alto Douro, UTAD, www.utad.pt, 5001
Center for the Research and Technology of Agro-Environmental and Biological
Sciences (CITAB), UTAD, www.utad.pt, 5001-911, Vila Real, Portugal
Animal and Veterinary Research Center (CECAV), School of Agrarian and
Veterinary Sciences, UTAD, www.utad.pt, 5001-911, Vila Real, Portugal
Organic Chemistry of Natural Products and Agrifood (QOPNA), Mass
Spectrometry Center, Department of Chemistry, University of Aveiro,
193, Aveiro, Portugal
* anafaustino.faustino@sapo.pt
Introduction and aims: Development of spontaneous mammary tumors in rats is
he first year of life. The present work describes a spontaneous
mammary carcinoma in a 7-week-old female rat.
Thirty-four Sprague-Dawley female rats with four weeks of age
were used in an assay of chemically-induced mammary carcinogenesis. After one week
of quarantine, animals were randomly divided into five experimental groups and were
allowed to acclimate to the lab conditions for two weeks. All procedures followed the
National and European legislation, and were approved by National (Ap
008961) and University (CE_12-2013) Ethics Committees.
No alterations were found during the quarantine or acclimatization period. However,
at fifty days of age, one animal from control group exhibited a movable mass in the left
The mass grown quickly and the animal health status deteriorated faster.
Eight days later, the animal was humanely sacrificed. A complete necropsy was
performed. The mass cut surface was reddish in appearance with several cysts filled
. The mass was immersed in buffered formalin and routinely
processed for light microscopy. It was stained with hematoxylin and eosin (H&E),
Schiff (PAS) and immunohistochemically evaluated by staining with an
antibody against estrogen receptor (ER)-α. The tumor was histologically classified as a
Meeting of the Portuguese Society of Animal Pathology Beyound Routine Diagnosis, 20 and 21 of May 2016
22
, Ginja M.1,2
Veterinary Sciences,
Montes and Alto Douro, UTAD, www.utad.pt, 5001-911, Vila
Environmental and Biological
gal
Animal and Veterinary Research Center (CECAV), School of Agrarian and
911, Vila Real, Portugal
Organic Chemistry of Natural Products and Agrifood (QOPNA), Mass
ry, University of Aveiro,
Development of spontaneous mammary tumors in rats is
he first year of life. The present work describes a spontaneous
Dawley female rats with four weeks of age
s. After one week
of quarantine, animals were randomly divided into five experimental groups and were
allowed to acclimate to the lab conditions for two weeks. All procedures followed the
National and European legislation, and were approved by National (Approval no.
No alterations were found during the quarantine or acclimatization period. However,
at fifty days of age, one animal from control group exhibited a movable mass in the left
The mass grown quickly and the animal health status deteriorated faster.
Eight days later, the animal was humanely sacrificed. A complete necropsy was
performed. The mass cut surface was reddish in appearance with several cysts filled
. The mass was immersed in buffered formalin and routinely
processed for light microscopy. It was stained with hematoxylin and eosin (H&E),
Schiff (PAS) and immunohistochemically evaluated by staining with an
. The tumor was histologically classified as a
high-grade undifferentiated carcinoma originated in the mammary gland. It was
negative for PAS staining and positive for ER
Conclusion: This is the first report describing a spontaneous mammary tu
Sprague-Dawley rat with less than 10 weeks old. No previous reports had described a
spontaneous mammary tumor in such a young rat.
Funding: This work was supported by European Investment Funds by
FEDER/COMPETE/POCI
Program, under Project POCI
Science and Technology (FCT), under the projec
PTDC/DES/114122/2009 and post
XXI Meeting of the Portuguese Society of Animal PathologyBeyound Routine Diagnosis, 20 and 21 of May 2016
grade undifferentiated carcinoma originated in the mammary gland. It was
negative for PAS staining and positive for ER-α.
This is the first report describing a spontaneous mammary tu
Dawley rat with less than 10 weeks old. No previous reports had described a
spontaneous mammary tumor in such a young rat.
This work was supported by European Investment Funds by
FEDER/COMPETE/POCI - Operational Competitiveness and Internationalization
Program, under Project POCI-01-0145-FEDER-006958 and Portuguese Foundation for
Science and Technology (FCT), under the project UID/AGR/04033/2013, the project
PTDC/DES/114122/2009 and post-graduation grant SFRH/BD/102099/2014.
Meeting of the Portuguese Society of Animal Pathology Beyound Routine Diagnosis, 20 and 21 of May 2016
23
grade undifferentiated carcinoma originated in the mammary gland. It was
This is the first report describing a spontaneous mammary tumor in a
Dawley rat with less than 10 weeks old. No previous reports had described a
This work was supported by European Investment Funds by
Operational Competitiveness and Internationalization
006958 and Portuguese Foundation for
t UID/AGR/04033/2013, the project
graduation grant SFRH/BD/102099/2014.
Pathological study of two cases of mortality in Iberian
introduced in Portugal in the years 2015
Valente P.1, Correia J.J.
Serra R.4 e Peleteiro M.C.2
1 Bolseira do CNPq, Brasil, Faculdade de Medicina Veterinária, Universidade de
Lisboa. 2 Centro Interdisciplinar de Investigação em Sanidade Animal (CIISA), Faculdade de
Medicina Veterinária, Universidade de Lisboa.3 Instituto da Conservação da Natureza e das Florestas, Lisboa.4 Centro Nacional de Reprodução de Lince Ibérico, São Bartolomeu de Messines.
* jcorreia@fmv.ulisboa.pt
In Portugal, the Iberian-
as an "endangered" species. Currently, the Iberian
through LIFE+Iberlince reintroduction program (LIFE+10NAT/ES/570), using
born in captivity.
This study presents two cases of Iberian
identified as Case A, (Kayakweru) female, two years, and Case B, (Myrtilis) female one
year.
The Case A, presented moderate weight loss, dehydration,
blood, discreet hydrothorax, congested and edematous lung and congestion of liver,
spleen and kidneys. The stomach contained undigested lung fragments, from which was
held positive drug test for strychnine.
The Case B had very pronoun
the stomach. Histopathology interstitial pneumonia, necrotic enteritis, and lymphoid
hypoplasia in the spleen and mesenteric lymph nodes were seen together with
degeneration and necrosis of liver and
panleucopenia virus.
We conclude that, in Case A, death was due to poisoning by strychnine and in Case
B to infection by feline panleucopenia virus.
Among the animals introduced in Portugal/Spain these were the fi
strychnine poisoning and the first recorded death by feline panleucopenia.
XXI Meeting of the Portuguese Society of Animal PathologyBeyound Routine Diagnosis, 20 and 21 of May 2016
Pathological study of two cases of mortality in Iberian-lynx (Lynx pardinus
introduced in Portugal in the years 2015-16
, Correia J.J.2*, Moreira A.2, Duarte A.2, Carrapiço B.2, Fernandes M.
Bolseira do CNPq, Brasil, Faculdade de Medicina Veterinária, Universidade de
Centro Interdisciplinar de Investigação em Sanidade Animal (CIISA), Faculdade de
edicina Veterinária, Universidade de Lisboa.
Instituto da Conservação da Natureza e das Florestas, Lisboa.
Centro Nacional de Reprodução de Lince Ibérico, São Bartolomeu de Messines.
oa.pt
-lynx was considered virtually extinct and classified globally
as an "endangered" species. Currently, the Iberian-lynx is being recovered in Portugal,
through LIFE+Iberlince reintroduction program (LIFE+10NAT/ES/570), using
This study presents two cases of Iberian-lynxes, who died after reintroduction,
identified as Case A, (Kayakweru) female, two years, and Case B, (Myrtilis) female one
The Case A, presented moderate weight loss, dehydration, incompletely clotted
blood, discreet hydrothorax, congested and edematous lung and congestion of liver,
spleen and kidneys. The stomach contained undigested lung fragments, from which was
held positive drug test for strychnine.
The Case B had very pronounced weight loss, near cachexia, with no signs of food in
the stomach. Histopathology interstitial pneumonia, necrotic enteritis, and lymphoid
hypoplasia in the spleen and mesenteric lymph nodes were seen together with
degeneration and necrosis of liver and kidneys. Virology was positive for feline
We conclude that, in Case A, death was due to poisoning by strychnine and in Case
B to infection by feline panleucopenia virus.
Among the animals introduced in Portugal/Spain these were the first known case of
strychnine poisoning and the first recorded death by feline panleucopenia.
Meeting of the Portuguese Society of Animal Pathology Beyound Routine Diagnosis, 20 and 21 of May 2016
24
Lynx pardinus) re-
, Fernandes M.3,
Bolseira do CNPq, Brasil, Faculdade de Medicina Veterinária, Universidade de
Centro Interdisciplinar de Investigação em Sanidade Animal (CIISA), Faculdade de
Centro Nacional de Reprodução de Lince Ibérico, São Bartolomeu de Messines.
lynx was considered virtually extinct and classified globally
lynx is being recovered in Portugal,
through LIFE+Iberlince reintroduction program (LIFE+10NAT/ES/570), using animals
lynxes, who died after reintroduction,
identified as Case A, (Kayakweru) female, two years, and Case B, (Myrtilis) female one
incompletely clotted
blood, discreet hydrothorax, congested and edematous lung and congestion of liver,
spleen and kidneys. The stomach contained undigested lung fragments, from which was
ced weight loss, near cachexia, with no signs of food in
the stomach. Histopathology interstitial pneumonia, necrotic enteritis, and lymphoid
hypoplasia in the spleen and mesenteric lymph nodes were seen together with
kidneys. Virology was positive for feline
We conclude that, in Case A, death was due to poisoning by strychnine and in Case
rst known case of
strychnine poisoning and the first recorded death by feline panleucopenia.
The anatomic-pathologic diagnosis proves to be a valuable tool in determining the
cause of death of the Iberian lynx, as well as in the process of reassessment of
measures in order to reduce appropriately mortality from other Lynxes, thus increasing
the success of recovery project of the species.
XXI Meeting of the Portuguese Society of Animal PathologyBeyound Routine Diagnosis, 20 and 21 of May 2016
pathologic diagnosis proves to be a valuable tool in determining the
cause of death of the Iberian lynx, as well as in the process of reassessment of
measures in order to reduce appropriately mortality from other Lynxes, thus increasing
the success of recovery project of the species.
Meeting of the Portuguese Society of Animal Pathology Beyound Routine Diagnosis, 20 and 21 of May 2016
25
pathologic diagnosis proves to be a valuable tool in determining the
cause of death of the Iberian lynx, as well as in the process of reassessment of control
measures in order to reduce appropriately mortality from other Lynxes, thus increasing
Liver and spleen infiltration with round cells in a cat
Peleteiro M.C.
CIISA, Interdisciplinary Centre of
Medicina Veterinária, Universidade de Lisboa, Av. da Universidade Técnica, 1300
Lisboa, Portugal (PR, HP, SC, SJ, MCP)
* mcpelet@fmv.ulisboa.pt
The present case refers to a consented necropsy performed in a cat with no known
clinical history.
Identification – Feline, sterilized
breed.
The necropsy revealed:
Discrete emaciation; severely hypertrophic
milimetric whitish foci dispersed throughout the whole organ; the same type of foci
could be seen in the spleen, together with a larger nodule 2
hypertrophy of the mesenteric lymph nodes; severe at
congestion and edema of the lungs.
Histopathology revealed:
Marked infiltration of the liver and spleen with round cells, with acidophilic finely
granular cytoplasm, and small eccentric nucleus. Very few hepatic cells remain in t
liver. In the lumen of blood vessels in both organs, the same round cells can be seen.
The mesenteric lymph node showed edema and lymphoid cell hypoplasia with no
evidence of follicles. No microbial agents were identified.
What is your diagnosis?
XXI Meeting of the Portuguese Society of Animal PathologyBeyound Routine Diagnosis, 20 and 21 of May 2016
Liver and spleen infiltration with round cells in a cat
CIISA, Interdisciplinary Centre of Research in Animal Health, Faculdade de
Medicina Veterinária, Universidade de Lisboa, Av. da Universidade Técnica, 1300
Lisboa, Portugal (PR, HP, SC, SJ, MCP)
* mcpelet@fmv.ulisboa.pt
The present case refers to a consented necropsy performed in a cat with no known
Feline, sterilized female, estimated age - 9 years
Discrete emaciation; severely hypertrophic liver with round borders and a myriad of
milimetric whitish foci dispersed throughout the whole organ; the same type of foci
could be seen in the spleen, together with a larger nodule 2.5 cm in diameter;
hypertrophy of the mesenteric lymph nodes; severe atrophy of the left kidney;
congestion and edema of the lungs.
Histopathology revealed:
Marked infiltration of the liver and spleen with round cells, with acidophilic finely
granular cytoplasm, and small eccentric nucleus. Very few hepatic cells remain in t
liver. In the lumen of blood vessels in both organs, the same round cells can be seen.
The mesenteric lymph node showed edema and lymphoid cell hypoplasia with no
No microbial agents were identified.
Meeting of the Portuguese Society of Animal Pathology Beyound Routine Diagnosis, 20 and 21 of May 2016
26
Research in Animal Health, Faculdade de
Medicina Veterinária, Universidade de Lisboa, Av. da Universidade Técnica, 1300-477
The present case refers to a consented necropsy performed in a cat with no known
9 years, undetermined
liver with round borders and a myriad of
milimetric whitish foci dispersed throughout the whole organ; the same type of foci
5 cm in diameter;
rophy of the left kidney;
Marked infiltration of the liver and spleen with round cells, with acidophilic finely
granular cytoplasm, and small eccentric nucleus. Very few hepatic cells remain in the
liver. In the lumen of blood vessels in both organs, the same round cells can be seen.
The mesenteric lymph node showed edema and lymphoid cell hypoplasia with no
Fine-needle aspirate cytology of a mass posterior to the eye
Lemos E.1*, Pinto da Cunha N.1Centro Diagnóstico Veterinário 2 Faculdade de Medicina Veterinária
*evasalemos@gmail.com
A 15-year-old neutered female dog of undefined breed presented with clinical signs
on the left eye: exoftalmy, third eyelid protusion, ocular pain, dilated episcleral vessels
and purulent conjunctival secretion. The patient also felt pain on opening the mouth.
During the ecography, a mass posterior to the left eye was identified. A complete blood
cell count (CBC) in the ADVIA 210 hematology analiser and a serum chemistry profile
were performed, as well as fine
globe.
The CBC revealed mild normocytic normochromic anemia, apparently not
regenerative after blood smear evaluation. Biochemistry profile indicated mild urea
elevation (81.2 mg/dL; Reference Interval [RI] 15
UI/L; RI 10-90 UI/L) and ALP (694 UI/L; RI <120 UI/L).
The cytology showed sparse to moderate cellularity with a proteinaceous
background. There was a mixed population of cells distributed in
the same cell type, occasionally forming sequential layers containing: cells with high
relation nucleus: cytoplasm and cleaved nucleus with dense chromatin; cells with
abundant cytoplasm containing numerous needle
central or peripheral nucleus; cells with abundant eosinophilic cytoplasm and round
nucleus; and large cells with slightly basophilic cytoplasm with indefined boundaries,
with central nucleus of reticular chromatin and evident nucleolus. All of
populations were relatively monomorphic without obvious signs of cytologic atypia.
There were also frequently lysed cells.
The animal was treated with anti
To date the dog presents no clinical
XXI Meeting of the Portuguese Society of Animal PathologyBeyound Routine Diagnosis, 20 and 21 of May 2016
needle aspirate cytology of a mass posterior to the eye
*, Pinto da Cunha N.1,2
Centro Diagnóstico Veterinário – CEDIVET, Porto, Portugal;
Faculdade de Medicina Veterinária – ULHT, Lisboa, Portugal
evasalemos@gmail.com;
old neutered female dog of undefined breed presented with clinical signs
on the left eye: exoftalmy, third eyelid protusion, ocular pain, dilated episcleral vessels
onjunctival secretion. The patient also felt pain on opening the mouth.
During the ecography, a mass posterior to the left eye was identified. A complete blood
cell count (CBC) in the ADVIA 210 hematology analiser and a serum chemistry profile
ed, as well as fine-needle aspirate cytology of the mass behind the ocular
The CBC revealed mild normocytic normochromic anemia, apparently not
regenerative after blood smear evaluation. Biochemistry profile indicated mild urea
; Reference Interval [RI] 15.0-68.5 mg/dL), as well as ALT (184
90 UI/L) and ALP (694 UI/L; RI <120 UI/L).
The cytology showed sparse to moderate cellularity with a proteinaceous
background. There was a mixed population of cells distributed in groups, each one of
the same cell type, occasionally forming sequential layers containing: cells with high
cytoplasm and cleaved nucleus with dense chromatin; cells with
abundant cytoplasm containing numerous needle-shaped melanin granule
central or peripheral nucleus; cells with abundant eosinophilic cytoplasm and round
nucleus; and large cells with slightly basophilic cytoplasm with indefined boundaries,
with central nucleus of reticular chromatin and evident nucleolus. All of
populations were relatively monomorphic without obvious signs of cytologic atypia.
There were also frequently lysed cells.
The animal was treated with anti-inflammatory and antibiotic and the mass receded.
To date the dog presents no clinical signs of ocular pathology.
Meeting of the Portuguese Society of Animal Pathology Beyound Routine Diagnosis, 20 and 21 of May 2016
27
old neutered female dog of undefined breed presented with clinical signs
on the left eye: exoftalmy, third eyelid protusion, ocular pain, dilated episcleral vessels
onjunctival secretion. The patient also felt pain on opening the mouth.
During the ecography, a mass posterior to the left eye was identified. A complete blood
cell count (CBC) in the ADVIA 210 hematology analiser and a serum chemistry profile
needle aspirate cytology of the mass behind the ocular
The CBC revealed mild normocytic normochromic anemia, apparently not
regenerative after blood smear evaluation. Biochemistry profile indicated mild urea
5 mg/dL), as well as ALT (184
The cytology showed sparse to moderate cellularity with a proteinaceous
groups, each one of
the same cell type, occasionally forming sequential layers containing: cells with high
cytoplasm and cleaved nucleus with dense chromatin; cells with
shaped melanin granules and round
central or peripheral nucleus; cells with abundant eosinophilic cytoplasm and round
nucleus; and large cells with slightly basophilic cytoplasm with indefined boundaries,
with central nucleus of reticular chromatin and evident nucleolus. All of these cell
populations were relatively monomorphic without obvious signs of cytologic atypia.
inflammatory and antibiotic and the mass receded.
Multiple myeloma: atypical presentation with cutaneous metastases
Marcos R.1, Canadas A
Santos M.1
1Cytology Diagnostic Services, Instituto de Ciências Biomédicas de Abel Salazar,
ICBAS-UP, University of Porto 2 Laboratory of Veterinary Pathology, ICBAS3 UPVET, ICBAS-UP
rmarcos@icbas.up.pt
A 9-year-old Golden Retriever
and lameness. On physical examination, inguinal papules and regional
lymphadenopathies were found.
The first diagnostic workup included fine
complete blood count and biochemical analysis. The dog had bicytopenia (moderate
normocytic normochromic anaemia and severe thrombocytopenia) and FNA presented
round cells (some binucleated) with 10
cytoplasmatic eosinophilic border
bodies were observed in the background. Round atypical cells were also detected in
buffy-coat smears. The cytological features
cell neoplasia, suggestive of multip
Biopsy of papules, cytology of bone marrow and popliteal lymph node were
out. Additionally, cell blocks (cytotube technique) of buffy
performed. Bone marrow was characterized by a large proportion of plasmacyt
including flame-cells. These atypical cells were observed in lymph node.
Histopathological examination of papules revealed multifocal infiltrates of round cells
with high pleomorphism and nuclear atypia, negative to CD3, PAX
intense staining for MUM-
coat and bone marrow cell blocks. The
axial skeleton and diffuse changes in liver and spleen. A final diagnosis of multiple
myeloma was made.
XXI Meeting of the Portuguese Society of Animal PathologyBeyound Routine Diagnosis, 20 and 21 of May 2016
Multiple myeloma: atypical presentation with cutaneous metastases
, Canadas A.2, Leite-Martins L.3, Ribeiro J.3, Santos J.3
Cytology Diagnostic Services, Instituto de Ciências Biomédicas de Abel Salazar,
UP, University of Porto
Laboratory of Veterinary Pathology, ICBAS-UP
n Retriever was referred with polydypsia-polyuria, weight loss
physical examination, inguinal papules and regional
lymphadenopathies were found.
The first diagnostic workup included fine-needle aspiration (FNA) of papules,
count and biochemical analysis. The dog had bicytopenia (moderate
normocytic normochromic anaemia and severe thrombocytopenia) and FNA presented
round cells (some binucleated) with 10-20 µm diameter and, occasionally, with
cytoplasmatic eosinophilic border. Granular eosinophilic material and lymphoglandular
bodies were observed in the background. Round atypical cells were also detected in
coat smears. The cytological features were consistent with disseminated round
cell neoplasia, suggestive of multiple myeloma.
Biopsy of papules, cytology of bone marrow and popliteal lymph node were
out. Additionally, cell blocks (cytotube technique) of buffy-coat and bone marrow were
Bone marrow was characterized by a large proportion of plasmacyt
cells. These atypical cells were observed in lymph node.
Histopathological examination of papules revealed multifocal infiltrates of round cells
with high pleomorphism and nuclear atypia, negative to CD3, PAX-5, CD18, and with
-1. Immunolabeling for MUM-1 was also present in buffy
coat and bone marrow cell blocks. The imaging study revealed osteolytic lesions in
axial skeleton and diffuse changes in liver and spleen. A final diagnosis of multiple
Meeting of the Portuguese Society of Animal Pathology Beyound Routine Diagnosis, 20 and 21 of May 2016
28
Multiple myeloma: atypical presentation with cutaneous metastases 3, de Matos A.3,
Cytology Diagnostic Services, Instituto de Ciências Biomédicas de Abel Salazar,
polyuria, weight loss
physical examination, inguinal papules and regional
needle aspiration (FNA) of papules,
count and biochemical analysis. The dog had bicytopenia (moderate
normocytic normochromic anaemia and severe thrombocytopenia) and FNA presented
m diameter and, occasionally, with
. Granular eosinophilic material and lymphoglandular
bodies were observed in the background. Round atypical cells were also detected in
were consistent with disseminated round
Biopsy of papules, cytology of bone marrow and popliteal lymph node were carried
coat and bone marrow were
Bone marrow was characterized by a large proportion of plasmacytoid cells,
cells. These atypical cells were observed in lymph node.
Histopathological examination of papules revealed multifocal infiltrates of round cells
5, CD18, and with
1 was also present in buffy-
imaging study revealed osteolytic lesions in
axial skeleton and diffuse changes in liver and spleen. A final diagnosis of multiple
A case of T-cell-rich B-
Pires M.A.1,2; Gama A.1,2
C.2,3
1CECAV – Departamento de Ciências Veterinárias, Laboratório de Histologia e
Anatomia Patológica, UTAD, 50002 Hospital Veterinário da UTAD. Departamento de Ciências Veterinárias, 5000
Vila Real. 3 CITAB – Centre for the Research and Tec
of Agro-Environmental and Biological Sciences, University of Trás
Alto Douro, Vila Real, Portugal
*apires@utad.pt
A sample was received in the Laboratory of Histology and Anatomical Pathology
from an incisional biopsy of a gingival mass located in the rostral mandible. It was
sampled from a female mongrel
The sample had 1cm of diameter consisted of white and brown tissues.
The histological appearance ha
presented with proliferation of round cells arranged on solid nests that invaded blood
vessels (which have vasculitis and thrombosis) as well as the nerves and the adjacent
tissues that were crushed in th
low mitotic index.
The cells had the morphology of small lymphocytes and immunoblasts. It was noted
as well the presence of many linfoglandular bodies.
The samples were immunostained for lymphocytes
polyclonal BLA.36, Dako) and macrophages (monoclonal MCA 387, Serotec). The
immunostaining was markedly positive for T lymphocytes (CD3), small cells and
regular in their shape and size, present in the periphery of the lesion.
positivity to B lymphocytes as the larger and aberrant cells with atypical nuclei.
Macrophages were present in small agglomerates.
The phenotypic view was rated as T
undergoing chemotherapy.
XXI Meeting of the Portuguese Society of Animal PathologyBeyound Routine Diagnosis, 20 and 21 of May 2016
-cell lymphoma in a dog 1,2; Seixas F.1,2; Pinto M.L.1,2; Santos T.2; Lebre F
Departamento de Ciências Veterinárias, Laboratório de Histologia e
Anatomia Patológica, UTAD, 5000-801 Vila Real.
da UTAD. Departamento de Ciências Veterinárias, 5000
for the Research and Technology
Environmental and Biological Sciences, University of Trás
Alto Douro, Vila Real, Portugal
A sample was received in the Laboratory of Histology and Anatomical Pathology
incisional biopsy of a gingival mass located in the rostral mandible. It was
sampled from a female mongrel dog that presented with hyporexia and oral pain.
had 1cm of diameter consisted of white and brown tissues.
The histological appearance had no identifiable tissue from the anatomic area and
presented with proliferation of round cells arranged on solid nests that invaded blood
vessels (which have vasculitis and thrombosis) as well as the nerves and the adjacent
that were crushed in the surgical margin. The tissues also showed necrosis and a
The cells had the morphology of small lymphocytes and immunoblasts. It was noted
as well the presence of many linfoglandular bodies.
The samples were immunostained for lymphocytes (monoclonal anti
polyclonal BLA.36, Dako) and macrophages (monoclonal MCA 387, Serotec). The
was markedly positive for T lymphocytes (CD3), small cells and
regular in their shape and size, present in the periphery of the lesion.
positivity to B lymphocytes as the larger and aberrant cells with atypical nuclei.
Macrophages were present in small agglomerates.
The phenotypic view was rated as T-cell-rich B-cell lymphoma. The animal is
Meeting of the Portuguese Society of Animal Pathology Beyound Routine Diagnosis, 20 and 21 of May 2016
29
; Lebre F.2; Viegas
Departamento de Ciências Veterinárias, Laboratório de Histologia e
da UTAD. Departamento de Ciências Veterinárias, 5000-801
Environmental and Biological Sciences, University of Trás-os-Montes e
A sample was received in the Laboratory of Histology and Anatomical Pathology
incisional biopsy of a gingival mass located in the rostral mandible. It was
dog that presented with hyporexia and oral pain.
had 1cm of diameter consisted of white and brown tissues.
d no identifiable tissue from the anatomic area and
presented with proliferation of round cells arranged on solid nests that invaded blood
vessels (which have vasculitis and thrombosis) as well as the nerves and the adjacent
e surgical margin. The tissues also showed necrosis and a
The cells had the morphology of small lymphocytes and immunoblasts. It was noted
(monoclonal anti-CD3 and
polyclonal BLA.36, Dako) and macrophages (monoclonal MCA 387, Serotec). The
was markedly positive for T lymphocytes (CD3), small cells and
regular in their shape and size, present in the periphery of the lesion. There was also
positivity to B lymphocytes as the larger and aberrant cells with atypical nuclei.
cell lymphoma. The animal is
CASE REPORT: MEDULOBLASTOMA NUM CÃO COM TRÊS ANOS
Ruivo P.1, Monteiro C.A.A.
1 CIISA, Interdisciplinary Centre of Research in Animal Health, Faculdade de
Medicina Veterinária, Universidade de Lisboa, Av
Lisboa, Portugal (PR, HP, SC, SJ, MCP)2 Hospital Escolar Veterinário, Faculdade de Medicina Veterinária, Universidade de
Lisboa, Av. da Universidade Técnica, 1300
* ruivo_pedro@hotmail.com
A three year-old male Labrador dog was brought to the Veterinary Teaching Hospital
with complaints of dysphagia, vomiting, cough and lameness of the right hind limb. At
physical examination Horner’s Syndrome was diagnosed and hypertrophy of the right
popliteal lymph node was noted. Cytology revealed numerous round cells with scant
cytoplasm and dense nucleus, suggestive of lymphoma. Biopsy of the same lymph node
identified invasion of most of its structure by compact cells with round to spindled
nucleus; immunohistochemistry was negative for CD3 and CD20, excluding the
lymphoma hypothesis. One week later the animal suffered a convulsive episode and the
cranial CT scan revealed a multif
cerebellum´s left hemisphere. The deterioration of the clinical condition led the owners
to opt for euthanasia. At necropsy lymphadenomegaly of the left retropharyngeal and
iliacs was detected. A 2x1cm lardac
hemorrhagic friable mass occupied almost all the cerebellum´s left hemisphere.
Histologically, the encephalon’s masses and the lymph nodes presented the same
tumoral tissue as the right popliteal, correspondin
shaped cells with hyperchromatic nucleus, arranged in solid lobules separated by a scant
stroma, characteristic of an embryonal tumor. The immunohistochemical study was
positive for synaptophysin and negative for GF
These results allowed us to diagnose this neoplasia as a medulloblastoma with
neuroblastic and not glial (astrocytic) differentiation, with meningeal and lymphatic
dissemination
XXI Meeting of the Portuguese Society of Animal PathologyBeyound Routine Diagnosis, 20 and 21 of May 2016
PORT: MEDULOBLASTOMA NUM CÃO COM TRÊS ANOS
C.A.A.2, Pissarra H.1, Carvalho S.1, Jesus S.1,2, Peleteiro
CIISA, Interdisciplinary Centre of Research in Animal Health, Faculdade de
Medicina Veterinária, Universidade de Lisboa, Av. da Universidade Técnica, 1300
Lisboa, Portugal (PR, HP, SC, SJ, MCP)
Hospital Escolar Veterinário, Faculdade de Medicina Veterinária, Universidade de
Lisboa, Av. da Universidade Técnica, 1300-477 Lisboa, Portugal (CAAM, SJ)
* ruivo_pedro@hotmail.com
old male Labrador dog was brought to the Veterinary Teaching Hospital
with complaints of dysphagia, vomiting, cough and lameness of the right hind limb. At
ination Horner’s Syndrome was diagnosed and hypertrophy of the right
popliteal lymph node was noted. Cytology revealed numerous round cells with scant
cytoplasm and dense nucleus, suggestive of lymphoma. Biopsy of the same lymph node
most of its structure by compact cells with round to spindled
nucleus; immunohistochemistry was negative for CD3 and CD20, excluding the
lymphoma hypothesis. One week later the animal suffered a convulsive episode and the
cranial CT scan revealed a multifocal lesion, involving the optic chiasm and the
cerebellum´s left hemisphere. The deterioration of the clinical condition led the owners
to opt for euthanasia. At necropsy lymphadenomegaly of the left retropharyngeal and
iliacs was detected. A 2x1cm lardaceous mass was involving the optic chiasm and a
hemorrhagic friable mass occupied almost all the cerebellum´s left hemisphere.
Histologically, the encephalon’s masses and the lymph nodes presented the same
tumoral tissue as the right popliteal, corresponding to a proliferation of spindle to round
shaped cells with hyperchromatic nucleus, arranged in solid lobules separated by a scant
stroma, characteristic of an embryonal tumor. The immunohistochemical study was
positive for synaptophysin and negative for GFAP, with 100% of positive cells to Ki67.
These results allowed us to diagnose this neoplasia as a medulloblastoma with
neuroblastic and not glial (astrocytic) differentiation, with meningeal and lymphatic
Meeting of the Portuguese Society of Animal Pathology Beyound Routine Diagnosis, 20 and 21 of May 2016
30
PORT: MEDULOBLASTOMA NUM CÃO COM TRÊS ANOS
, Peleteiro M.C.1
CIISA, Interdisciplinary Centre of Research in Animal Health, Faculdade de
. da Universidade Técnica, 1300-477
Hospital Escolar Veterinário, Faculdade de Medicina Veterinária, Universidade de
477 Lisboa, Portugal (CAAM, SJ)
old male Labrador dog was brought to the Veterinary Teaching Hospital
with complaints of dysphagia, vomiting, cough and lameness of the right hind limb. At
ination Horner’s Syndrome was diagnosed and hypertrophy of the right
popliteal lymph node was noted. Cytology revealed numerous round cells with scant
cytoplasm and dense nucleus, suggestive of lymphoma. Biopsy of the same lymph node
most of its structure by compact cells with round to spindled
nucleus; immunohistochemistry was negative for CD3 and CD20, excluding the
lymphoma hypothesis. One week later the animal suffered a convulsive episode and the
ocal lesion, involving the optic chiasm and the
cerebellum´s left hemisphere. The deterioration of the clinical condition led the owners
to opt for euthanasia. At necropsy lymphadenomegaly of the left retropharyngeal and
eous mass was involving the optic chiasm and a
hemorrhagic friable mass occupied almost all the cerebellum´s left hemisphere.
Histologically, the encephalon’s masses and the lymph nodes presented the same
g to a proliferation of spindle to round
shaped cells with hyperchromatic nucleus, arranged in solid lobules separated by a scant
stroma, characteristic of an embryonal tumor. The immunohistochemical study was
AP, with 100% of positive cells to Ki67.
These results allowed us to diagnose this neoplasia as a medulloblastoma with
neuroblastic and not glial (astrocytic) differentiation, with meningeal and lymphatic
Establishment of antibody panels
tumour diagnosis in Veterinary Pathology
Pires M.A.1*; Gama A.
Vala H.3,4; Delgado L.5; Faísca P
Monteiro, M.9; Amorim I.
R.12; Alves A.1
1CECAV – Departamento de Ciências Veterinárias, Laboratório de Histologia e
Anatomia Patológica, UTAD, 50002ICAAM, Departamento
Mitra, Ap. 94, 7002-554 Évora, Portugal3Agrarian School of Viseu, Polytechnic Institute of Viseu. 4CITAB and CI&DETS, Portugal 5Laboratório Veterinário INNO. Portugal6Faculdade de Medicina Veterinária da Universidade Lusófona de Humanidades e
Tecnologia, Lisboa, Portugal7DNATech, Lisboa, Portugal8INIAV, Laboratório de Patologia, Vairão, Portugal9INIAV, UEISPSA, Laboratório de Patologia, Lisboa, Portugal10ICBAS, Institute of Biomedical Sciences Abel Salazar; Instituto de Investigação e
Inovação em Saúde (I3S), Institute of Molecular Pathology and Immunology
(IPATIMUP) of the University of Porto, Portugal11Faculdade de Medicina Veterinária12 CEDIVET - Centro Diagnóstico Veterinário Lda, Portugal
* apires@utad.pt
In Portugal, Veterinary Pathology is developing rapidly, and in recent years we assist
to the emergence of private laboratories and the restruct
polytechnics and public laboratories.The Portuguese Society of Animal Pathology,
through its actions and its associates has been keeping the discussion among its peers in
order to standardizethe criteriaof description, classification
which are the subject of our daily work.
One of the last challenges is associated with the use of routine histochemical
techniques and immunohistochemistry, in an effort to establish standardized panels for
XXI Meeting of the Portuguese Society of Animal PathologyBeyound Routine Diagnosis, 20 and 21 of May 2016
Establishment of antibody panels and histochemical techniques in routine
tumour diagnosis in Veterinary Pathology
.1; Pires I.1; Seixas F.1; Prada J.1; Pinto M.
; Faísca P.6,7; Carvalho., I7; Lima C.8, Ochoa C
.10, Gartner F.10; Peleteiro C.11; Carvalho S.
Departamento de Ciências Veterinárias, Laboratório de Histologia e
Anatomia Patológica, UTAD, 5000-801 Vila Real. Portugal
ICAAM, Departamento de Medicina Veterinária, Universidade de Évora. Pólo da
554 Évora, Portugal
Agrarian School of Viseu, Polytechnic Institute of Viseu. Viseu, Portugal
CITAB and CI&DETS, Portugal
Laboratório Veterinário INNO. Portugal
edicina Veterinária da Universidade Lusófona de Humanidades e
Tecnologia, Lisboa, Portugal
DNATech, Lisboa, Portugal
INIAV, Laboratório de Patologia, Vairão, Portugal
INIAV, UEISPSA, Laboratório de Patologia, Lisboa, Portugal
Biomedical Sciences Abel Salazar; Instituto de Investigação e
Inovação em Saúde (I3S), Institute of Molecular Pathology and Immunology
(IPATIMUP) of the University of Porto, Portugal
Faculdade de Medicina Veterinária–Universidade de Lisboa, Portugal
Centro Diagnóstico Veterinário Lda, Portugal
In Portugal, Veterinary Pathology is developing rapidly, and in recent years we assist
to the emergence of private laboratories and the restructuring of universities,
polytechnics and public laboratories.The Portuguese Society of Animal Pathology,
through its actions and its associates has been keeping the discussion among its peers in
order to standardizethe criteriaof description, classification and evaluation of cases
which are the subject of our daily work.
One of the last challenges is associated with the use of routine histochemical
techniques and immunohistochemistry, in an effort to establish standardized panels for
Meeting of the Portuguese Society of Animal Pathology Beyound Routine Diagnosis, 20 and 21 of May 2016
31
and histochemical techniques in routine
.L.1; Branco S.2;
, Ochoa C.8; Orge L.9,
.11; Gil da Costa
Departamento de Ciências Veterinárias, Laboratório de Histologia e
de Medicina Veterinária, Universidade de Évora. Pólo da
Viseu, Portugal
edicina Veterinária da Universidade Lusófona de Humanidades e
Biomedical Sciences Abel Salazar; Instituto de Investigação e
Inovação em Saúde (I3S), Institute of Molecular Pathology and Immunology
Universidade de Lisboa, Portugal
In Portugal, Veterinary Pathology is developing rapidly, and in recent years we assist
uring of universities,
polytechnics and public laboratories.The Portuguese Society of Animal Pathology,
through its actions and its associates has been keeping the discussion among its peers in
and evaluation of cases
One of the last challenges is associated with the use of routine histochemical
techniques and immunohistochemistry, in an effort to establish standardized panels for
tumour diagnosis, which could eventually reduce each analysis cost.
simple survey was built, in which all collaborators answered questions about the
markers used for carcinoma, sarcoma and round cell tumour diagnosis, as well as
general questions related with t
questions, from public and private laboratories.
immunohistochemical and histochemical methods are used for diagnosis.
spectrum cytokeratins are universally used to confi
sarcoma. The CD3 marker is used by all laboratories to identify T lymphocytes. For the
diagnosis of B-cell lymphoma, the marker used is not consensual. In each laboratory
there are different markers for more specific situatio
techniques for diagnosis. These data will be presented to promote extended discussion,
namely to reach a consensus when different markers are used.
XXI Meeting of the Portuguese Society of Animal PathologyBeyound Routine Diagnosis, 20 and 21 of May 2016
could eventually reduce each analysis cost. For this purpose a
simple survey was built, in which all collaborators answered questions about the
markers used for carcinoma, sarcoma and round cell tumour diagnosis, as well as
general questions related with the subject. We obtained twenty-one answered to the
questions, from public and private laboratories. In general, in most cases
immunohistochemical and histochemical methods are used for diagnosis.
spectrum cytokeratins are universally used to confirm carcinoma, and vimentin for
sarcoma. The CD3 marker is used by all laboratories to identify T lymphocytes. For the
lymphoma, the marker used is not consensual. In each laboratory
there are different markers for more specific situations and only two labs perform PCR
These data will be presented to promote extended discussion,
namely to reach a consensus when different markers are used.
Meeting of the Portuguese Society of Animal Pathology Beyound Routine Diagnosis, 20 and 21 of May 2016
32
For this purpose a
simple survey was built, in which all collaborators answered questions about the
markers used for carcinoma, sarcoma and round cell tumour diagnosis, as well as
one answered to the
In general, in most cases
immunohistochemical and histochemical methods are used for diagnosis. The wide
rm carcinoma, and vimentin for
sarcoma. The CD3 marker is used by all laboratories to identify T lymphocytes. For the
lymphoma, the marker used is not consensual. In each laboratory
ns and only two labs perform PCR
These data will be presented to promote extended discussion,
Poster presentations
XXI Meeting of the Portuguese Society of Animal PathologyBeyound Routine Diagnosis, 20 and 21 of May 2016
Poster presentations
Meeting of the Portuguese Society of Animal Pathology Beyound Routine Diagnosis, 20 and 21 of May 2016
33
1. Cyclooxygenase-2 expression
mice
Arantes-Rodrigues R.1,2,3*,
1Centre for the Research and Technology of Agro
Sciences (CITAB), University of Trás
Portugal 2QOPNA, Mass Spectrometry Center, Department of Chemistry, University of
Aveiro, Portugal 3Laboratory Animal Science Group, Institute for Research and Innovation in Health
(I3S), Porto, Portugal 4Laboratory for Process Engineer
(LEPABE), Faculty of Engineering, University of Porto, Portugal5Molecular Oncology and Viral Pathology Group, Portuguese Institute of Oncology,
Porto, Portugal 6Department of Veterinary Sciences,
*arantesregina@hotmail.com
Cyclooxygenase-2 (COX
urinary bladder cancer detection, but also for prediction of disease progression and
prognosis. The present study investigated the expression of COX
preneoplastic and neoplastic lesions induced by the oral administration of N
hydroxybutyl) nitrosamine (BBN).
The study comprised two groups of ICR animals: group 1 (control)
(which received BBN over the course of 12 weeks). Urothelial lesions were analyzed by
haematoxylin and eosin staining. COX
distribution and intensity.
In negative control mice COX
urothelium and scattered leukocytes in the lamina propria, assuming a diffuse
cytoplasmic pattern. Dysplastic lesions showed multifocal to coalescing COX
positivity, with positive cells in all epithelial layers. The s
showed multifocal positivity in 10
XXI Meeting of the Portuguese Society of Animal PathologyBeyound Routine Diagnosis, 20 and 21 of May 2016
2 expression during urinary bladder carcinogenesis in ICR
1,2,3*, Gil da Costa R.M.4,5, Oliveira P.A.1,6
Centre for the Research and Technology of Agro-Environmental and Biological
Sciences (CITAB), University of Trás-os-Montes and Alto Douro (UTAD)
QOPNA, Mass Spectrometry Center, Department of Chemistry, University of
Laboratory Animal Science Group, Institute for Research and Innovation in Health
Laboratory for Process Engineering, Environment, Biotechnology and Energy
(LEPABE), Faculty of Engineering, University of Porto, Portugal
Molecular Oncology and Viral Pathology Group, Portuguese Institute of Oncology,
Department of Veterinary Sciences, UTAD, Portugal
arantesregina@hotmail.com
2 (COX-2) is considered a promising molecular marker not only for
urinary bladder cancer detection, but also for prediction of disease progression and
present study investigated the expression of COX-2 in mouse urothelial
preneoplastic and neoplastic lesions induced by the oral administration of N
hydroxybutyl) nitrosamine (BBN).
The study comprised two groups of ICR animals: group 1 (control)
(which received BBN over the course of 12 weeks). Urothelial lesions were analyzed by
haematoxylin and eosin staining. COX-2 immunostaining was assessed according to its
In negative control mice COX-2 expression was restricted to umbrella cells in the
urothelium and scattered leukocytes in the lamina propria, assuming a diffuse
cytoplasmic pattern. Dysplastic lesions showed multifocal to coalescing COX
positivity, with positive cells in all epithelial layers. The single invasive carcinoma
showed multifocal positivity in 10-25% of cells.
Meeting of the Portuguese Society of Animal Pathology Beyound Routine Diagnosis, 20 and 21 of May 2016
34
arcinogenesis in ICR
Environmental and Biological
(UTAD), Vila Real,
QOPNA, Mass Spectrometry Center, Department of Chemistry, University of
Laboratory Animal Science Group, Institute for Research and Innovation in Health
ing, Environment, Biotechnology and Energy
Molecular Oncology and Viral Pathology Group, Portuguese Institute of Oncology,
2) is considered a promising molecular marker not only for
urinary bladder cancer detection, but also for prediction of disease progression and
2 in mouse urothelial
preneoplastic and neoplastic lesions induced by the oral administration of N-butyl-N-(4-
The study comprised two groups of ICR animals: group 1 (control) and group 2
(which received BBN over the course of 12 weeks). Urothelial lesions were analyzed by
2 immunostaining was assessed according to its
restricted to umbrella cells in the
urothelium and scattered leukocytes in the lamina propria, assuming a diffuse
cytoplasmic pattern. Dysplastic lesions showed multifocal to coalescing COX-2
ingle invasive carcinoma
Altered COX-2 expression may be indicative of tumour progression in BBN induced
urinary bladder cancer.
XXI Meeting of the Portuguese Society of Animal PathologyBeyound Routine Diagnosis, 20 and 21 of May 2016
2 expression may be indicative of tumour progression in BBN induced
Meeting of the Portuguese Society of Animal Pathology Beyound Routine Diagnosis, 20 and 21 of May 2016
35
2 expression may be indicative of tumour progression in BBN induced
2. Ptaquiloside from bracken (Pteridium spp.) inhibits tumour
CD8+ T cells in HPV-transgenic mice.
Santos C.1, Ferreirinha P.
T.1,2,6, Oliveira P.A.6,7, Medeiros R.
1 ICBAS, Instituto de Ciências Biomédicas de Abel
Rua de Jorge Viterbo Ferreira 228, 40502 Grupo de Oncologia Molecular e Patologia Viral, CI
Oncologia do Porto, Rua Dr. António Bernardino de Almeida, 42003 Serviço de Virologia, Instituto Português de Oncologia do Porto, Rua Dr. António
Bernardino de Almeida, 42004 Liga Portuguesa Contra o Cancro
Circunvalação, nº. 6657, 42005 LEPABE, Faculdade de Engenharia, Universidade do Porto, Rua Dr. Roberto Frias
s/n, 4200-465 Porto, Portugal6 Centro de Investigação e Tecnologia de Ciências Agroambientais e Biológicas
(CITAB), Universidade de Trás
5001-911 Vila Real, Portugal7 Departamento de Ciências Veterinárias, Universidade de Trás
Douro, UTAD, Quinta de Prados, 50018 CEBIMED, Faculdade de Ciências da Saúde, Universidade Fernando Pes
Portugal
*rmcosta@fe.up.pt
Bracken (Pteridium sp.) is a globally
Exposure to bracken toxins like ptaquiloside is hypothesized to increase the risk of
papillomavirus-related upper digestive cancers. Ptaquiloside is thought to be
immunotoxic, facilitating the development of viral lesions. We used a human
papillomavirus type 16-transgenic (K14
ptaquiloside on tumour-infiltrating CD8+ T lymphocytes, which are critical players
against virus and tumour cells.
HPV16+/- mice were orally administered ptaquiloside (0.5mg/mouse/week) during
10 weeks and euthanized at 30 weeks
XXI Meeting of the Portuguese Society of Animal PathologyBeyound Routine Diagnosis, 20 and 21 of May 2016
Ptaquiloside from bracken (Pteridium spp.) inhibits tumour
transgenic mice.
, Ferreirinha P.1, Sousa H.2,3, Ribeiro J.2,3,4, Bastos M.M.S.M.
, Medeiros R.1,2,3,4,8, Vilanova M.1, Gil da Costa R.M.
ICBAS, Instituto de Ciências Biomédicas de Abel Salazar, Universidade do Porto,
Rua de Jorge Viterbo Ferreira 228, 4050-313 Porto, Portugal
Grupo de Oncologia Molecular e Patologia Viral, CI-IPOP, Instituto Português de
Oncologia do Porto, Rua Dr. António Bernardino de Almeida, 4200-072 Porto, Portuga
Serviço de Virologia, Instituto Português de Oncologia do Porto, Rua Dr. António
Bernardino de Almeida, 4200-072 Porto, Portugal
Liga Portuguesa Contra o Cancro — Núcleo Regional do Norte, Estrada Interior da
. 6657, 4200-177 Porto, Portugal
LEPABE, Faculdade de Engenharia, Universidade do Porto, Rua Dr. Roberto Frias
465 Porto, Portugal
Centro de Investigação e Tecnologia de Ciências Agroambientais e Biológicas
(CITAB), Universidade de Trás-os-Montes e Alto Douro, UTAD, Quinta de Prados,
911 Vila Real, Portugal
Departamento de Ciências Veterinárias, Universidade de Trás-os
Douro, UTAD, Quinta de Prados, 5001-801 Vila Real, Portugal
CEBIMED, Faculdade de Ciências da Saúde, Universidade Fernando Pes
Bracken (Pteridium sp.) is a globally-distributed weed, widely present in pastures.
Exposure to bracken toxins like ptaquiloside is hypothesized to increase the risk of
related upper digestive cancers. Ptaquiloside is thought to be
ic, facilitating the development of viral lesions. We used a human
transgenic (K14-HPV16) mouse model to study the effects of
infiltrating CD8+ T lymphocytes, which are critical players
cells.
mice were orally administered ptaquiloside (0.5mg/mouse/week) during
10 weeks and euthanized at 30 weeks-old, together with matched untreated controls.
Meeting of the Portuguese Society of Animal Pathology Beyound Routine Diagnosis, 20 and 21 of May 2016
36
Ptaquiloside from bracken (Pteridium spp.) inhibits tumour-infiltrating
, Bastos M.M.S.M.5, Neto
, Gil da Costa R.M.2,5.
Salazar, Universidade do Porto,
IPOP, Instituto Português de
072 Porto, Portugal
Serviço de Virologia, Instituto Português de Oncologia do Porto, Rua Dr. António
Núcleo Regional do Norte, Estrada Interior da
LEPABE, Faculdade de Engenharia, Universidade do Porto, Rua Dr. Roberto Frias
Centro de Investigação e Tecnologia de Ciências Agroambientais e Biológicas
, Quinta de Prados,
os-Montes e Alto
CEBIMED, Faculdade de Ciências da Saúde, Universidade Fernando Pessoa, Porto,
distributed weed, widely present in pastures.
Exposure to bracken toxins like ptaquiloside is hypothesized to increase the risk of
related upper digestive cancers. Ptaquiloside is thought to be
ic, facilitating the development of viral lesions. We used a human
HPV16) mouse model to study the effects of
infiltrating CD8+ T lymphocytes, which are critical players
mice were orally administered ptaquiloside (0.5mg/mouse/week) during
old, together with matched untreated controls.
Skin samples were enzymatically digested and CD8+ T cells analysed for CD107a and
CD44 surface expression.
Ptaquiloside-exposed HPV16+/
percentage of CD8+CD107a+ and CD8+CD44+ T cells compared with untreated
HPV16+/- animals and a trend towards reduced CD8+ T cell infiltration. Histologica
100% of ptaquiloside-treated mice showed diffuse epidermal dysplasia, compared with
50% untreated mice.
These findings indicate that ptaquiloside exerts an immunosuppressive role by
decreasing CD8+ T cell memory activation and degranulation in HPV
Given the key role of CD8+ T lymphocytes against papillomavirus
effect is likely to contribute for viral persistence and tumour progression.
XXI Meeting of the Portuguese Society of Animal PathologyBeyound Routine Diagnosis, 20 and 21 of May 2016
Skin samples were enzymatically digested and CD8+ T cells analysed for CD107a and
exposed HPV16+/- animals showed a significantly (P < 0.05) reduced
percentage of CD8+CD107a+ and CD8+CD44+ T cells compared with untreated
animals and a trend towards reduced CD8+ T cell infiltration. Histologica
treated mice showed diffuse epidermal dysplasia, compared with
These findings indicate that ptaquiloside exerts an immunosuppressive role by
decreasing CD8+ T cell memory activation and degranulation in HPV-
Given the key role of CD8+ T lymphocytes against papillomavirus-induced lesions, this
effect is likely to contribute for viral persistence and tumour progression.
Meeting of the Portuguese Society of Animal Pathology Beyound Routine Diagnosis, 20 and 21 of May 2016
37
Skin samples were enzymatically digested and CD8+ T cells analysed for CD107a and
animals showed a significantly (P < 0.05) reduced
percentage of CD8+CD107a+ and CD8+CD44+ T cells compared with untreated
animals and a trend towards reduced CD8+ T cell infiltration. Histologically,
treated mice showed diffuse epidermal dysplasia, compared with
These findings indicate that ptaquiloside exerts an immunosuppressive role by
-induced lesions.
induced lesions, this
effect is likely to contribute for viral persistence and tumour progression.
3. Extraskeletal sarcomas: Retrospective study of 5 years (2011Delgado L.* 1, Silva C. 1
1 Laboratório INNO, Serviços Especializados em Veterinária, Braga, Portugal.2 CECAV, Universidade de Trás
* mldelgado.mv@gmail.com
The extraskeletal sarcomas (ESS) are mesenchymal neoplasms characterized by the
production of bone and cartilage, without association to the skeletal system or periosteal
tissue. These are rare neoplasms in dogs, and even more rare
uncommon in humans. The biological behavior is usually aggressive, with high rates of
local recurrence and metastasis.
The histopathology reports of confirmed extraskeletal sarcomas diagnosed between
January 2011 and March 2016 w
computer system (Clinidata XXI of Maxdata®). The main analyzed features were:
species, gender, breed, age, tumor location and diagnosis.
Over a period of 5 years, 7 cases of ESS have been diagnosed, 5 i
feline species.
In dogs, there were 4 female and 1 male with an average age of 11.2 years, with
tumors located in abdominal region (n=2) and subcutaneous (n=5). In cats, we found 2
cases of subcutaneous tumors, both were females, with 10 an
Clinical data of these cases did not report a history of trauma or inoculation or evidence
of bone involvement.
In literature, there are few cases described in both dogs and cats, the affected animals
are usually geriatric with no
In this five years period, we conclude that the ESS are extremely rare neoplasms,
particularly in cats. Our sample is in agreement with the literature in terms of
epidemiological data, however we found more cases at c
visceral locations.
XXI Meeting of the Portuguese Society of Animal PathologyBeyound Routine Diagnosis, 20 and 21 of May 2016
Extraskeletal sarcomas: Retrospective study of 5 years (2011-2016)1, Oliveira J.P. 2, Simões P.B. 1
Laboratório INNO, Serviços Especializados em Veterinária, Braga, Portugal.
CECAV, Universidade de Trás-os-Montes e Alto Douro, Vila Real, Portugal.
mldelgado.mv@gmail.com
The extraskeletal sarcomas (ESS) are mesenchymal neoplasms characterized by the
production of bone and cartilage, without association to the skeletal system or periosteal
tissue. These are rare neoplasms in dogs, and even more rare in felines. They are also
uncommon in humans. The biological behavior is usually aggressive, with high rates of
local recurrence and metastasis.
The histopathology reports of confirmed extraskeletal sarcomas diagnosed between
January 2011 and March 2016 were reviewed. Data were collected from the laboratory
computer system (Clinidata XXI of Maxdata®). The main analyzed features were:
species, gender, breed, age, tumor location and diagnosis.
Over a period of 5 years, 7 cases of ESS have been diagnosed, 5 i
In dogs, there were 4 female and 1 male with an average age of 11.2 years, with
tumors located in abdominal region (n=2) and subcutaneous (n=5). In cats, we found 2
cases of subcutaneous tumors, both were females, with 10 and 11 years, respectively.
Clinical data of these cases did not report a history of trauma or inoculation or evidence
In literature, there are few cases described in both dogs and cats, the affected animals
are usually geriatric with no apparent sex or breed predisposition.
In this five years period, we conclude that the ESS are extremely rare neoplasms,
particularly in cats. Our sample is in agreement with the literature in terms of
epidemiological data, however we found more cases at cutaneous sites rather than
Meeting of the Portuguese Society of Animal Pathology Beyound Routine Diagnosis, 20 and 21 of May 2016
38
2016)
Laboratório INNO, Serviços Especializados em Veterinária, Braga, Portugal.
Montes e Alto Douro, Vila Real, Portugal.
The extraskeletal sarcomas (ESS) are mesenchymal neoplasms characterized by the
production of bone and cartilage, without association to the skeletal system or periosteal
in felines. They are also
uncommon in humans. The biological behavior is usually aggressive, with high rates of
The histopathology reports of confirmed extraskeletal sarcomas diagnosed between
ere reviewed. Data were collected from the laboratory
computer system (Clinidata XXI of Maxdata®). The main analyzed features were:
Over a period of 5 years, 7 cases of ESS have been diagnosed, 5 in dogs and 2 in
In dogs, there were 4 female and 1 male with an average age of 11.2 years, with
tumors located in abdominal region (n=2) and subcutaneous (n=5). In cats, we found 2
d 11 years, respectively.
Clinical data of these cases did not report a history of trauma or inoculation or evidence
In literature, there are few cases described in both dogs and cats, the affected animals
In this five years period, we conclude that the ESS are extremely rare neoplasms,
particularly in cats. Our sample is in agreement with the literature in terms of
utaneous sites rather than
4. OvisOme: an updated database for Ovis aries
Esteves E.1, Fernandes M
1 Universidade Católica Portuguesa, CIIS
em Saúde, Instituto de Ciências da Saúde 2 Centre for the Research and Technology of Agro
Sciences (CITAB and Centre for Studies in Education, and Health Technologies
(CI&DETS), Portugal 3 Centro de Investigação e de Tecnologias Agro
Centro de Estudos em Educação, Tecnologias e Saúde (CI&DETS).
Portugal.
* hvala2@gmail.com;
Introduction: The Omics sciences are part of the research and diagnostic routines in
human health. However, their application in veterinary sciences is still sparse, albeit the
increasing number of proteomics studies published, especially regarding farm animals.
The amount of information accumulated by these high throughput techniques, makes
the existence of specialized databases fundamental. These databases are essential to
store, annotate and make available to the scientific community, all the information
gathered by the different omics studies, so that researchers can use it to understand the
physio pathological mechanisms underlying sheep diseases, as well as to develop new
and improved diagnostic, prognostic and therapeutic strategies.
Objective: The aim of this wo
demonstrate how it can be used to understand the molecular mechanisms underlying
sheep disease.
Methodologies: OvisOme compiles all proteins identified by proteomics studies of
Ovis aries. The proteins are anno
techniques used and all the data the authors refer regarding the donor sheep’s health.
Results: The database currently has 1451 proteins, associated to 8 diseases and 10
breeds. When compared to othe
more information than other databases not specific for sheep, such as UniProt.
XXI Meeting of the Portuguese Society of Animal PathologyBeyound Routine Diagnosis, 20 and 21 of May 2016
OvisOme: an updated database for Ovis aries
, Fernandes M.1, Rosa N.1, Correia M.J.1, Vala H.2*, Barros M
Universidade Católica Portuguesa, CIIS – Centro de Investigação Interdisciplinar
em Saúde, Instituto de Ciências da Saúde – Viseu, Viseu, Portugal;
Centre for the Research and Technology of Agro-Environmental and Biological
Sciences (CITAB and Centre for Studies in Education, and Health Technologies
vestigação e de Tecnologias Agro-Ambientais e Biológicas (CITAB) e
Centro de Estudos em Educação, Tecnologias e Saúde (CI&DETS).
The Omics sciences are part of the research and diagnostic routines in
human health. However, their application in veterinary sciences is still sparse, albeit the
increasing number of proteomics studies published, especially regarding farm animals.
ount of information accumulated by these high throughput techniques, makes
the existence of specialized databases fundamental. These databases are essential to
store, annotate and make available to the scientific community, all the information
the different omics studies, so that researchers can use it to understand the
physio pathological mechanisms underlying sheep diseases, as well as to develop new
and improved diagnostic, prognostic and therapeutic strategies.
The aim of this work is to present the OvisOme database and to
demonstrate how it can be used to understand the molecular mechanisms underlying
OvisOme compiles all proteins identified by proteomics studies of
Ovis aries. The proteins are annotated as to the sample characterization, the proteomics
techniques used and all the data the authors refer regarding the donor sheep’s health.
The database currently has 1451 proteins, associated to 8 diseases and 10
breeds. When compared to other proteomics databases, the OvisOme stores and displays
more information than other databases not specific for sheep, such as UniProt.
Meeting of the Portuguese Society of Animal Pathology Beyound Routine Diagnosis, 20 and 21 of May 2016
39
, Barros M.1
Centro de Investigação Interdisciplinar
Environmental and Biological
Sciences (CITAB and Centre for Studies in Education, and Health Technologies
Ambientais e Biológicas (CITAB) e
Centro de Estudos em Educação, Tecnologias e Saúde (CI&DETS). ESAV-IPV.
The Omics sciences are part of the research and diagnostic routines in
human health. However, their application in veterinary sciences is still sparse, albeit the
increasing number of proteomics studies published, especially regarding farm animals.
ount of information accumulated by these high throughput techniques, makes
the existence of specialized databases fundamental. These databases are essential to
store, annotate and make available to the scientific community, all the information
the different omics studies, so that researchers can use it to understand the
physio pathological mechanisms underlying sheep diseases, as well as to develop new
rk is to present the OvisOme database and to
demonstrate how it can be used to understand the molecular mechanisms underlying
OvisOme compiles all proteins identified by proteomics studies of
tated as to the sample characterization, the proteomics
techniques used and all the data the authors refer regarding the donor sheep’s health.
The database currently has 1451 proteins, associated to 8 diseases and 10
r proteomics databases, the OvisOme stores and displays
more information than other databases not specific for sheep, such as UniProt.
Conclusion: OvisOme is a valuable tool for the study of the molecular mechanisms
underlying sheep health and disease.
XXI Meeting of the Portuguese Society of Animal PathologyBeyound Routine Diagnosis, 20 and 21 of May 2016
OvisOme is a valuable tool for the study of the molecular mechanisms
underlying sheep health and disease.
Meeting of the Portuguese Society of Animal Pathology Beyound Routine Diagnosis, 20 and 21 of May 2016
40
OvisOme is a valuable tool for the study of the molecular mechanisms
5. A case of Myxosarcoma in a Chilean Flamingo (
Zoologycal Collection "Quinta dos Plátanos
Pessoa, V.1,2, Melo, P.3, Ramada, M.
e Correia, J.J.1
1CIISA, Faculdade de Medicina Veterinária, Universidade de Lisboa.2Quinta dos plátanos, Abrantes.3VetNatura, Lisboa.
* verapuri@gmail.com
This was a case study reported in a Chilean Flamingo (
zoo collection, "Quinta dos Plátanos", in Abrantes
There isn´t still a great knowledge of cancer disease in avian medicine specially
regarding diagnostic and treatment, and for so reporting cases like this, can be of
extreme importance. Myxosarcomas are even more uncommon and can occur in
different regions of the body, rich in conjunctive tissue.
It was a 3 year old female flamingo, with microchip number 967000009551840,
which presented a mass located over the first finger jo
cm of diameter, exophytic, ulcerated, highly vascular and with a soft consistency. The
first approach was to surgically remove the mass, sending it conserved in 10% formalin
solution, to the Pathology Laboratory of the FM
histopathology technique. The diagnostic was myxosarcoma. After the first surgery the
tumor developed again, so the decision was to amputate the left wing at the level of the
humeral-radio ulnar joint.
Malignant tumors are not very common in flamingos (Phoenicopteridae), though
retrospective studies indicate that they have similar prevalence when compared with
other birds. This specific type of tumor, myxosarcoma, has a low metastasis rate, though
it may recidivate after surgery.
This may be the first case of myxosarcoma in a Chilean flamingo’s wing (
Chilensi), and for so it is an original and so far a successful case.
XXI Meeting of the Portuguese Society of Animal PathologyBeyound Routine Diagnosis, 20 and 21 of May 2016
A case of Myxosarcoma in a Chilean Flamingo (Phoenicopterus chilensis
ollection "Quinta dos Plátanos
, Ramada, M.3, Rodrigues, J.P.2, Escusa, S.1, Carvalho, L.M.
Medicina Veterinária, Universidade de Lisboa.
Quinta dos plátanos, Abrantes.
This was a case study reported in a Chilean Flamingo (P. Chilensis), from a private
collection, "Quinta dos Plátanos", in Abrantes – Portugal, during October of 2015.
There isn´t still a great knowledge of cancer disease in avian medicine specially
regarding diagnostic and treatment, and for so reporting cases like this, can be of
importance. Myxosarcomas are even more uncommon and can occur in
different regions of the body, rich in conjunctive tissue.
It was a 3 year old female flamingo, with microchip number 967000009551840,
which presented a mass located over the first finger joint of the left wing, with about 6
cm of diameter, exophytic, ulcerated, highly vascular and with a soft consistency. The
first approach was to surgically remove the mass, sending it conserved in 10% formalin
solution, to the Pathology Laboratory of the FMV-ULisbon, for analysis by the routine
histopathology technique. The diagnostic was myxosarcoma. After the first surgery the
tumor developed again, so the decision was to amputate the left wing at the level of the
s are not very common in flamingos (Phoenicopteridae), though
retrospective studies indicate that they have similar prevalence when compared with
other birds. This specific type of tumor, myxosarcoma, has a low metastasis rate, though
er surgery.
This may be the first case of myxosarcoma in a Chilean flamingo’s wing (
), and for so it is an original and so far a successful case.
Meeting of the Portuguese Society of Animal Pathology Beyound Routine Diagnosis, 20 and 21 of May 2016
41
Phoenicopterus chilensis) of a
, Carvalho, L.M.1
Medicina Veterinária, Universidade de Lisboa.
), from a private
Portugal, during October of 2015.
There isn´t still a great knowledge of cancer disease in avian medicine specially
regarding diagnostic and treatment, and for so reporting cases like this, can be of
importance. Myxosarcomas are even more uncommon and can occur in
It was a 3 year old female flamingo, with microchip number 967000009551840,
int of the left wing, with about 6
cm of diameter, exophytic, ulcerated, highly vascular and with a soft consistency. The
first approach was to surgically remove the mass, sending it conserved in 10% formalin
ULisbon, for analysis by the routine
histopathology technique. The diagnostic was myxosarcoma. After the first surgery the
tumor developed again, so the decision was to amputate the left wing at the level of the
s are not very common in flamingos (Phoenicopteridae), though
retrospective studies indicate that they have similar prevalence when compared with
other birds. This specific type of tumor, myxosarcoma, has a low metastasis rate, though
This may be the first case of myxosarcoma in a Chilean flamingo’s wing (P.
6. Stromal characterization of feline endometrial adenocarcinomas (FEA)
Radar A.1,2, Carvalho A.
Faísca P.2, Payan-Carreira R
1 CECAV-Universidade de Trás
Portugal 2 Faculdade de Medicina Veterinária da Universidade Lusófona de Humanidades e
Tecnologia, Lisboa, Portugal3 Escola Superior de Tecnologia da Saúde do Porto, Portugal
* apires@utad.pt
Feline adenocarcinoma of the
describes, and can even be present in young animals. The descriptions of these lesions
have increased in recent years aiming the improvement knowledge behavior as well the
prognosis prediction. The tumour s
behaviour and aggression.
In the current work it was used tree markers: vimentin and desmin which are
intermediate filaments of mesenchymal cells and alpha
microfilaments, associated
It was used samples of normal uterus and FEA belonging to the archived of
Laboratory of Histology and Anatomical Pathology of UTAD. Normal samples were
separated in two stages of oestrus cycle
Immunolabeling was evaluated based on its intensity.
Vimentin positivity was shown on epithelial cells in normal uterus, as well on
endometrial stroma. The FEA stroma intensity was greater than in the normal
endometrium.
Desmin and α-actin have almost
stroma and strong muscle positivity. Blood vessels were strongly and consistently
marked by α-actin and desmin shows intensely staining of myometrium than stroma and
blood vessels. Some tumours cells are inc
The invasion of muscle layers its one important feature in FEA. We note that desmin
is the best marker for identification of this invasion that will be usefully in more
complex cases of FEA diagnosis.
XXI Meeting of the Portuguese Society of Animal PathologyBeyound Routine Diagnosis, 20 and 21 of May 2016
Stromal characterization of feline endometrial adenocarcinomas (FEA)
, Carvalho A.1, Macedo J.1, Abreu M.1, Oliveira M. 1,4
Carreira R.1; Pires M.A.1,2*
Universidade de Trás-os-Montes e Alto Douro, 5000-
Faculdade de Medicina Veterinária da Universidade Lusófona de Humanidades e
Tecnologia, Lisboa, Portugal
Escola Superior de Tecnologia da Saúde do Porto, Portugal
Feline adenocarcinoma of the endometrium (FEA) are more common than literature
describes, and can even be present in young animals. The descriptions of these lesions
have increased in recent years aiming the improvement knowledge behavior as well the
prognosis prediction. The tumour stroma is an important component in cancer
In the current work it was used tree markers: vimentin and desmin which are
intermediate filaments of mesenchymal cells and alpha-actin that is a constituent of
microfilaments, associated with the smooth muscle.
It was used samples of normal uterus and FEA belonging to the archived of
Laboratory of Histology and Anatomical Pathology of UTAD. Normal samples were
separated in two stages of oestrus cycle (estrogenic phase and progestogen phase
Immunolabeling was evaluated based on its intensity.
Vimentin positivity was shown on epithelial cells in normal uterus, as well on
endometrial stroma. The FEA stroma intensity was greater than in the normal
actin have almost overlapping staining, with weak labelling of the
stroma and strong muscle positivity. Blood vessels were strongly and consistently
actin and desmin shows intensely staining of myometrium than stroma and
blood vessels. Some tumours cells are inconsistently positive for these two markers.
The invasion of muscle layers its one important feature in FEA. We note that desmin
is the best marker for identification of this invasion that will be usefully in more
complex cases of FEA diagnosis.
Meeting of the Portuguese Society of Animal Pathology Beyound Routine Diagnosis, 20 and 21 of May 2016
42
Stromal characterization of feline endometrial adenocarcinomas (FEA) 1,4, Saraiva A.L.1,
-801 Vila Real,
Faculdade de Medicina Veterinária da Universidade Lusófona de Humanidades e
endometrium (FEA) are more common than literature
describes, and can even be present in young animals. The descriptions of these lesions
have increased in recent years aiming the improvement knowledge behavior as well the
important component in cancer
In the current work it was used tree markers: vimentin and desmin which are
actin that is a constituent of
It was used samples of normal uterus and FEA belonging to the archived of
Laboratory of Histology and Anatomical Pathology of UTAD. Normal samples were
(estrogenic phase and progestogen phase).
Vimentin positivity was shown on epithelial cells in normal uterus, as well on
endometrial stroma. The FEA stroma intensity was greater than in the normal
overlapping staining, with weak labelling of the
stroma and strong muscle positivity. Blood vessels were strongly and consistently
actin and desmin shows intensely staining of myometrium than stroma and
onsistently positive for these two markers.
The invasion of muscle layers its one important feature in FEA. We note that desmin
is the best marker for identification of this invasion that will be usefully in more
7. Morphological an immunohistochemical characterization of the cat ovary
Carvalho A.1, Abreu M.
M.A.1*
1 CECAV-Universidade de Trás
2 Faculdade de Medicina
Tecnologia, Lisboa, Portugal
* apires@utad.pt
In the ovary, the alternation in dominance between follicles and corpora lutea
translates into a cyclical dynamics
morphology and immunohistochemical staining of the intermediate filaments was the
aim of this study, which used 53 representative sections of ovaries, grouped according
to them being either in the follicular or lut
determined based on the morphological aspect of the ovary and uterus; the estrogenic
phase being represented by the presence in the ovary of follicular growth and
nonspecific glands in the endometrium and the
lutea in the ovary and endometrium glands at its maximum expression.
Using immunohistochemical technique we studied the expression of three markers
using monoclonal antibodies: vimentin, a marker for mesenchymal cells, a
cytokeratins 7 (CK7) and 20 (CK20), markers for epithelial cells. Quantification of the
staining was done according to the intensity and percentage of positive cells.
Blood vessels, theca cells, stroma of ovaries and corpora lutea showed higher
intensity staining for vimentin, in contrast to the granulosa cells. The CK7 showed a
strong positive staining in the surface epithelium, while the other structures manifested
less intense staining. The CK20 showed a very weak positive staining in the surface
epithelium and higher intensity label
was during the estrogenic phase that
XXI Meeting of the Portuguese Society of Animal PathologyBeyound Routine Diagnosis, 20 and 21 of May 2016
ological an immunohistochemical characterization of the cat ovary
, Abreu M.1, Fortuna Cunha M.1, RadarA.1,2, Payan-Carreira R
Universidade de Trás-os-Montes e Alto Douro, 5000-801 Vila Real.
Faculdade de Medicina Veterinária da Universidade Lusófona de Humanidades e
Lisboa, Portugal
In the ovary, the alternation in dominance between follicles and corpora lutea
translates into a cyclical dynamics of sex hormones. The understanding of the
morphology and immunohistochemical staining of the intermediate filaments was the
aim of this study, which used 53 representative sections of ovaries, grouped according
to them being either in the follicular or luteal phase of the estrous cycle. This phase was
determined based on the morphological aspect of the ovary and uterus; the estrogenic
phase being represented by the presence in the ovary of follicular growth and
nonspecific glands in the endometrium and the progestagenic phase having corpora
lutea in the ovary and endometrium glands at its maximum expression.
Using immunohistochemical technique we studied the expression of three markers
using monoclonal antibodies: vimentin, a marker for mesenchymal cells, a
cytokeratins 7 (CK7) and 20 (CK20), markers for epithelial cells. Quantification of the
staining was done according to the intensity and percentage of positive cells.
Blood vessels, theca cells, stroma of ovaries and corpora lutea showed higher
y staining for vimentin, in contrast to the granulosa cells. The CK7 showed a
strong positive staining in the surface epithelium, while the other structures manifested
less intense staining. The CK20 showed a very weak positive staining in the surface
lium and higher intensity label in gin other structures. We found that, in general, it
uring the estrogenic phase that greater staining intensity in most structures was
Meeting of the Portuguese Society of Animal Pathology Beyound Routine Diagnosis, 20 and 21 of May 2016
43
ological an immunohistochemical characterization of the cat ovary
Carreira R1; Pires
801 Vila Real.
na de Humanidades e
In the ovary, the alternation in dominance between follicles and corpora lutea
of sex hormones. The understanding of the
morphology and immunohistochemical staining of the intermediate filaments was the
aim of this study, which used 53 representative sections of ovaries, grouped according
eal phase of the estrous cycle. This phase was
determined based on the morphological aspect of the ovary and uterus; the estrogenic
phase being represented by the presence in the ovary of follicular growth and
progestagenic phase having corpora
Using immunohistochemical technique we studied the expression of three markers
using monoclonal antibodies: vimentin, a marker for mesenchymal cells, and
cytokeratins 7 (CK7) and 20 (CK20), markers for epithelial cells. Quantification of the
staining was done according to the intensity and percentage of positive cells.
Blood vessels, theca cells, stroma of ovaries and corpora lutea showed higher
y staining for vimentin, in contrast to the granulosa cells. The CK7 showed a
strong positive staining in the surface epithelium, while the other structures manifested
less intense staining. The CK20 showed a very weak positive staining in the surface
in gin other structures. We found that, in general, it
greater staining intensity in most structures was
observed, with the corpus luteum being the structure which demonstrated more
reactivity across all markers used.
XXI Meeting of the Portuguese Society of Animal PathologyBeyound Routine Diagnosis, 20 and 21 of May 2016
observed, with the corpus luteum being the structure which demonstrated more
reactivity across all markers used.
Meeting of the Portuguese Society of Animal Pathology Beyound Routine Diagnosis, 20 and 21 of May 2016
44
observed, with the corpus luteum being the structure which demonstrated more
8. Comparative study of immunohistochemical expression of TGF
and canine mammary lesions
Pina J.1, Gama A.1,2, Seixas F.
1Laboratório de Histologia e Anatomia Patológica, Departamento de Ciências
Veterinárias, Universidade de Trás
Portugal 2Animal and Veterinary Research Centre (CECAV), UTAD, Vila Real, Portugal
* fseixas@utad.pt
Breast cancer is the most common type
in developing countries. In cats, breast tumors account for 12% of all tumors and 17%
of the tumors in females. In female dogs, spontaneous mammary tumors represents 25
to 50% of all neoplasia in this species. TGF
differentiation, motility, adhesion and cell death in almost all tissues and some studies
suggest that it functions both as a tumor suppressor and as tumor promoter. The aim of
this work is to contribute to the b
and dogs, more precisely regarding the expression of TGF
these tumors.
Immunohistochemical expression of TGF
stroma of 99 samples of feline mammary tissue and in 116 samples of canine mammary
tissue. In epithelial cells of feline mammary tissues, most of the histological groups
showed high expression of TGF
samples from the different hi
Regarding canine tissues, the expression of TGF
the samples, regardless of the histological group. In the stroma, statistically significant
associations were achieved (p <0
of TGFβ1 available in the medium that promotes tumor progression, but most likely is
the way it interacts with its receptors or other existing factors in the microenvironment.
XXI Meeting of the Portuguese Society of Animal PathologyBeyound Routine Diagnosis, 20 and 21 of May 2016
Comparative study of immunohistochemical expression of TGF
and canine mammary lesions
, Seixas F.1,2*
Laboratório de Histologia e Anatomia Patológica, Departamento de Ciências
rinárias, Universidade de Trás-os-Montes e Alto Douro, 5001
Animal and Veterinary Research Centre (CECAV), UTAD, Vila Real, Portugal
Breast cancer is the most common type of cancer in women, both in developed and
in developing countries. In cats, breast tumors account for 12% of all tumors and 17%
of the tumors in females. In female dogs, spontaneous mammary tumors represents 25
to 50% of all neoplasia in this species. TGFβ1 regulates processes such as division,
differentiation, motility, adhesion and cell death in almost all tissues and some studies
suggest that it functions both as a tumor suppressor and as tumor promoter. The aim of
this work is to contribute to the biopathological characterization of breast tumors in cats
and dogs, more precisely regarding the expression of TGFβ1 during the development of
Immunohistochemical expression of TGFβ1 was evaluated in the epithelium and
feline mammary tissue and in 116 samples of canine mammary
tissue. In epithelial cells of feline mammary tissues, most of the histological groups
showed high expression of TGFβ1. On the contrary, in the stroma, the majority of
samples from the different histological groups showed low expression of TGF
Regarding canine tissues, the expression of TGFβ1 in epithelial cells was low in most of
the samples, regardless of the histological group. In the stroma, statistically significant
d (p <0.0001). We verified that, probably, it is not the amount
1 available in the medium that promotes tumor progression, but most likely is
the way it interacts with its receptors or other existing factors in the microenvironment.
Meeting of the Portuguese Society of Animal Pathology Beyound Routine Diagnosis, 20 and 21 of May 2016
45
Comparative study of immunohistochemical expression of TGFβ1 in feline
Laboratório de Histologia e Anatomia Patológica, Departamento de Ciências
Montes e Alto Douro, 5001-801 Vila Real
Animal and Veterinary Research Centre (CECAV), UTAD, Vila Real, Portugal
of cancer in women, both in developed and
in developing countries. In cats, breast tumors account for 12% of all tumors and 17%
of the tumors in females. In female dogs, spontaneous mammary tumors represents 25
1 regulates processes such as division,
differentiation, motility, adhesion and cell death in almost all tissues and some studies
suggest that it functions both as a tumor suppressor and as tumor promoter. The aim of
thological characterization of breast tumors in cats
1 during the development of
1 was evaluated in the epithelium and
feline mammary tissue and in 116 samples of canine mammary
tissue. In epithelial cells of feline mammary tissues, most of the histological groups
1. On the contrary, in the stroma, the majority of
stological groups showed low expression of TGFβ1.
1 in epithelial cells was low in most of
the samples, regardless of the histological group. In the stroma, statistically significant
0001). We verified that, probably, it is not the amount
1 available in the medium that promotes tumor progression, but most likely is
the way it interacts with its receptors or other existing factors in the microenvironment.
9. Keratin 8 and 18 expression in normal uterus, cystic endometrial hyperplasia
(CEH) and feline endometrial adenocarcinoma (FEA)
Macedo J.1, RadarA.1,3,
R.1; PiresM.A.1,2*
1 CECAV-Universidade de2 Laboratório de Histologia e Anatomia Patológica, 3 Faculdade de Medicina Veterinária da Universidade Lusófona de Humanidades e
Tecnologia, 4Escola Superior de Tecnologia da Saúde do Porto
*apires@utad.pt
Keratin-18 (CK-18) and CK
diagnostic. This CK forming one of the pairs of intermediate filaments which constitute
the cytoskeleton of epithelial cells. CK
various cytoplasmic structures, and has been associated with various cellular processes
such as apoptosis, mitosis and signalling, and several metabolic pathways. The changes
of expression are associated with metabolic
associated with aggressiveness of certain cancers.
The CEH of the cat may be associated with an early stage of uterine cancer, in
particular with FEA, which are most common to the literature refers.
The aim of this study was to evaluate by immunohistochemistry the expression of
CK-8 and CK-18 in the cat oestrous cycle and in HQE and FEA.
It was used archival material from the Laboratory of Histology and Anatomical
Pathology of UTAD, eight uterus in estrogenic phase and 9
with CEH and 26 with FEA.
There was a non-significant decrease in CK
normal uterus. The expression of these CK in FEA also showed loss of positivity when
compared with the endometrium in estro
This study allowed us to conclude that the loss of expression of these CK 8/18 in
FEA and CEH of the cat may be associated with cell dedifferentiation and most severe
lesions. Nevertheless, follow
XXI Meeting of the Portuguese Society of Animal PathologyBeyound Routine Diagnosis, 20 and 21 of May 2016
nd 18 expression in normal uterus, cystic endometrial hyperplasia
(CEH) and feline endometrial adenocarcinoma (FEA)
, Carvalho A.1, Oliveira M.1,4, Saraiva A.L.1, Payan Carreira
Universidade de Trás-os-Montes e Alto Douro, 5000-801 Vila Real.
Laboratório de Histologia e Anatomia Patológica,
Faculdade de Medicina Veterinária da Universidade Lusófona de Humanidades e
Tecnologia, 4Escola Superior de Tecnologia da Saúde do Porto
18) and CK-8 are recognized epithelial markers for histopathology
diagnostic. This CK forming one of the pairs of intermediate filaments which constitute
the cytoskeleton of epithelial cells. CK-18 is associated with the flexible support of
various cytoplasmic structures, and has been associated with various cellular processes
such as apoptosis, mitosis and signalling, and several metabolic pathways. The changes
of expression are associated with metabolic and structural changes, and will be
associated with aggressiveness of certain cancers.
The CEH of the cat may be associated with an early stage of uterine cancer, in
particular with FEA, which are most common to the literature refers.
y was to evaluate by immunohistochemistry the expression of
18 in the cat oestrous cycle and in HQE and FEA.
It was used archival material from the Laboratory of Histology and Anatomical
Pathology of UTAD, eight uterus in estrogenic phase and 9 in progestagenic phase, 10
with CEH and 26 with FEA.
significant decrease in CK-18/8 expression in CEH related to a
normal uterus. The expression of these CK in FEA also showed loss of positivity when
compared with the endometrium in estrogenic and progestagenic stage.
This study allowed us to conclude that the loss of expression of these CK 8/18 in
FEA and CEH of the cat may be associated with cell dedifferentiation and most severe
lesions. Nevertheless, follow-up studies are needed to improve these findings.
Meeting of the Portuguese Society of Animal Pathology Beyound Routine Diagnosis, 20 and 21 of May 2016
46
nd 18 expression in normal uterus, cystic endometrial hyperplasia
, Payan Carreira
801 Vila Real.
Faculdade de Medicina Veterinária da Universidade Lusófona de Humanidades e
8 are recognized epithelial markers for histopathology
diagnostic. This CK forming one of the pairs of intermediate filaments which constitute
sociated with the flexible support of
various cytoplasmic structures, and has been associated with various cellular processes
such as apoptosis, mitosis and signalling, and several metabolic pathways. The changes
and structural changes, and will be
The CEH of the cat may be associated with an early stage of uterine cancer, in
y was to evaluate by immunohistochemistry the expression of
It was used archival material from the Laboratory of Histology and Anatomical
in progestagenic phase, 10
18/8 expression in CEH related to a
normal uterus. The expression of these CK in FEA also showed loss of positivity when
This study allowed us to conclude that the loss of expression of these CK 8/18 in
FEA and CEH of the cat may be associated with cell dedifferentiation and most severe
these findings.
10. In vitro and in vivo model for the study of Pcarcinogenesis
Figueira A.C.1,2,3, Gomes C.Gärtner F.2,3
1 Escola Universitária Vasco da Gama (EUVG), Coimbra, Portugal2 Instituto de Ciências Biomédicas Abel Salazar, Universidade do Porto (ICBAS
UP), Porto, Portugal 3 Instituto de Patologia e Imunologia Molecular da Universidade do Porto
(IPATIMUP), Porto, Portugal4 Centro de Estudos de Ciência Animal (CECA), Instituto de Ciências e Tecnologias
Agrárias e Agro Alimentares (ICETA), Universidade do Porto (UP), Porto, Po
* acfigueira@gmail.com
Mammary cancer in cats have high recurrence and metastatic potential. Abnormal
expression or function in the major molecules of the cadherin
have been related to breast cancer development and associated to cell migration,
invasion and metastatic dissemination. In feline mammary tumours, cadherins´ role is
still poorly known. Therefore we seek for suitable in vitro and in vivo model systems to
study the leading role of P
molecules from the cadherin
feline metastatic mammary carcinoma cell line (FMCm), by Western blot analysis,
immunofluorescence, imunop
FMCm cell line tumourigenic and metastatic capacity were assessed by orthotopically
inoculation of a cell suspension in the mammary fat pad of athymic nude mice
(N:NIH(S)II-nu/nu). Mice xenografts, as
immunohistochemically for cadherins expression. The FMCm cell line expressed E
P-cadherin. The FMCm cell line revealed to be high tumourigenic and showed a great
metastatic capacity as the orthotopic inoculat
primary and metastatic lesions in all mice. Those lesions expressed P
FMCm cell line can be proposed as a useful model for in vitro and in vivo studies of P
cadherin in feline mammary carcinoma pro
XXI Meeting of the Portuguese Society of Animal PathologyBeyound Routine Diagnosis, 20 and 21 of May 2016
In vitro and in vivo model for the study of P-cadherin in feline mammary
, Gomes C.3, Mendes N.3, de Matos A.J.F.2,4, Dias
Escola Universitária Vasco da Gama (EUVG), Coimbra, Portugal
Instituto de Ciências Biomédicas Abel Salazar, Universidade do Porto (ICBAS
Instituto de Patologia e Imunologia Molecular da Universidade do Porto
(IPATIMUP), Porto, Portugal
Centro de Estudos de Ciência Animal (CECA), Instituto de Ciências e Tecnologias
Agrárias e Agro Alimentares (ICETA), Universidade do Porto (UP), Porto, Po
acfigueira@gmail.com
Mammary cancer in cats have high recurrence and metastatic potential. Abnormal
expression or function in the major molecules of the cadherin-catenin adhesion complex
related to breast cancer development and associated to cell migration,
invasion and metastatic dissemination. In feline mammary tumours, cadherins´ role is
still poorly known. Therefore we seek for suitable in vitro and in vivo model systems to
eading role of P-cadherin in feline mammary carcinogenesis. Major
molecules from the cadherin-catenin complex (E- and P-cadherin) were evaluated in a
feline metastatic mammary carcinoma cell line (FMCm), by Western blot analysis,
immunofluorescence, imunoprecipitation and in situ proximity ligation assay. The
FMCm cell line tumourigenic and metastatic capacity were assessed by orthotopically
inoculation of a cell suspension in the mammary fat pad of athymic nude mice
nu/nu). Mice xenografts, as well as metastatic lesions, were evaluated
immunohistochemically for cadherins expression. The FMCm cell line expressed E
cadherin. The FMCm cell line revealed to be high tumourigenic and showed a great
metastatic capacity as the orthotopic inoculation in nude mice lead to the formation of
primary and metastatic lesions in all mice. Those lesions expressed P-
FMCm cell line can be proposed as a useful model for in vitro and in vivo studies of P
cadherin in feline mammary carcinoma progression.
Meeting of the Portuguese Society of Animal Pathology Beyound Routine Diagnosis, 20 and 21 of May 2016
47
cadherin in feline mammary
, Dias-Pereira P.2,
Instituto de Ciências Biomédicas Abel Salazar, Universidade do Porto (ICBAS-
Instituto de Patologia e Imunologia Molecular da Universidade do Porto
Centro de Estudos de Ciência Animal (CECA), Instituto de Ciências e Tecnologias
Agrárias e Agro Alimentares (ICETA), Universidade do Porto (UP), Porto, Portugal
Mammary cancer in cats have high recurrence and metastatic potential. Abnormal
catenin adhesion complex
related to breast cancer development and associated to cell migration,
invasion and metastatic dissemination. In feline mammary tumours, cadherins´ role is
still poorly known. Therefore we seek for suitable in vitro and in vivo model systems to
cadherin in feline mammary carcinogenesis. Major
cadherin) were evaluated in a
feline metastatic mammary carcinoma cell line (FMCm), by Western blot analysis,
recipitation and in situ proximity ligation assay. The
FMCm cell line tumourigenic and metastatic capacity were assessed by orthotopically
inoculation of a cell suspension in the mammary fat pad of athymic nude mice
well as metastatic lesions, were evaluated
immunohistochemically for cadherins expression. The FMCm cell line expressed E- and
cadherin. The FMCm cell line revealed to be high tumourigenic and showed a great
ion in nude mice lead to the formation of
and E-cadherin.
FMCm cell line can be proposed as a useful model for in vitro and in vivo studies of P-
11. Chrysosporium anamorph of
dragon (Pogona vitticeps)
Seixas F.1, Alvura N.2, Batista C.L.
1 CECAV – Departamento de Ciências Veterinárias, Laboratório de His
Anatomia Patológica, UTAD, 50002 Zoo da Maia, Rua da Estação, 4470
* fseixas@utad.pt
Bearded dragons (Pogona vitticeps
dermatological condition known in the pet trade as ‘‘yellow fungus disease” or “flesh
eater fungus”. The Yellow fungus disease is caused by the keratinophilic microfungus
Chrysosporium anamorph of
superficial and deep mycoses, and as fungi destroys the skin, the normal colouration of
the skin of the infected reptiles’ changes and may become yellowish, brownish, or even
black in later stages. Infections with
treated. Over the last years, this fungus has been shown to be an emerging pathogen in
captive reptiles, especially in bearded dragons and iguanas, but has also been identified
in wild reptiles. Human infection was
Herein, authors describe histopathological lesions observed in Bearded Dragon
infected with Chrysosporium
XXI Meeting of the Portuguese Society of Animal PathologyBeyound Routine Diagnosis, 20 and 21 of May 2016
anamorph of Nannizziopsis vriesii (CANV) lesions in bearded
, Batista C.L.2, Gama A1, Lourenço L1, Pires M.A.
Departamento de Ciências Veterinárias, Laboratório de His
Anatomia Patológica, UTAD, 5000-801 Vila Real
Zoo da Maia, Rua da Estação, 4470-178 Maia
Pogona vitticeps) kept in captivity suffer a sometimes fatal
dermatological condition known in the pet trade as ‘‘yellow fungus disease” or “flesh
eater fungus”. The Yellow fungus disease is caused by the keratinophilic microfungus
anamorph of Nannizziopsis vriessi (CANV). These fungi cause
superficial and deep mycoses, and as fungi destroys the skin, the normal colouration of
the skin of the infected reptiles’ changes and may become yellowish, brownish, or even
black in later stages. Infections with N. vreisii are contagious and often fatal if not
treated. Over the last years, this fungus has been shown to be an emerging pathogen in
captive reptiles, especially in bearded dragons and iguanas, but has also been identified
in wild reptiles. Human infection was also confirmed.
Herein, authors describe histopathological lesions observed in Bearded Dragon
Chrysosporium anamorph of Nannizziopsis vriesii.
Meeting of the Portuguese Society of Animal Pathology Beyound Routine Diagnosis, 20 and 21 of May 2016
48
(CANV) lesions in bearded
, Pires M.A.1
Departamento de Ciências Veterinárias, Laboratório de Histologia e
) kept in captivity suffer a sometimes fatal
dermatological condition known in the pet trade as ‘‘yellow fungus disease” or “flesh
eater fungus”. The Yellow fungus disease is caused by the keratinophilic microfungus
(CANV). These fungi cause
superficial and deep mycoses, and as fungi destroys the skin, the normal colouration of
the skin of the infected reptiles’ changes and may become yellowish, brownish, or even
are contagious and often fatal if not
treated. Over the last years, this fungus has been shown to be an emerging pathogen in
captive reptiles, especially in bearded dragons and iguanas, but has also been identified
Herein, authors describe histopathological lesions observed in Bearded Dragon
12. The histopathological timeframe of
development on bovines
Zúquete S.T.1*, Carvalho S.
Correia J.1, Cardoso L.A.1
1 Centro de Investigação Interdisciplinar em Sanidade Animal (CIISA), Faculdade de
Medicina Veterinária, Universidade de Lisboa2 Centro de Estudos de Vetores e Doenças Infeciosas Dr. Francisco Cambournac,
Instituto Nacional de Saúde Dr. Ricardo Jorge I.P., Águas de Moura, Portugal.
*sarazuquete@fmv.ulisboa.pt
Ticks are competent vectors of both
their meals by attaching to a host for several days. Even though some animals are
capable of acquiring some resistance to ticks after several infestations, tick attachment
to naïve cattle often result in skin
enough to induce efficient responses capable of leading to ticks skins detachment.
In order to characterize the recruitment of local inflammatory responses, two
Holstein-Frisea females were infested with l
adult ticks. Ticks remained secured inside tissue sleeves, which were glued to the
bovine dorsum. Sampling occurred at different time points throughout the complete
period of infestation, from the attachment of unfed t
engorged (fertilized) females. Skin biopsies (8 mm) were fixed in tamponed
formaldehyde (3.7%), embedded on paraffin blocks and later cut. Histological sections
had 3 μm and stained by hematoxylin & eosin coloration. The mic
of the biopsies allowed skin lesions identification. Both superficial and deep dermatitis,
as well as interstitial and follicular, with diverse diffuse and perivascular patterns were
found. An infiltration with predominance of inflamma
eosinophil cells) was registered. Some lymphoid cells were identified as well as rare
mast cells.
In conclusion, significant differences were found in samples obtained from control
and the infested animal, as well as among
engorgement allowing us to document different pathological changes due to this long
rostrum tick.
XXI Meeting of the Portuguese Society of Animal PathologyBeyound Routine Diagnosis, 20 and 21 of May 2016
12. The histopathological timeframe of Hyalomma lusitanicum
*, Carvalho S.1, Santos-Silva M.2, Santos A.S.2, Pereira da Fonseca I.
Centro de Investigação Interdisciplinar em Sanidade Animal (CIISA), Faculdade de
Medicina Veterinária, Universidade de Lisboa
Estudos de Vetores e Doenças Infeciosas Dr. Francisco Cambournac,
Instituto Nacional de Saúde Dr. Ricardo Jorge I.P., Águas de Moura, Portugal.
sarazuquete@fmv.ulisboa.pt
Ticks are competent vectors of both animal and human pathogens. Hard ticks secure
their meals by attaching to a host for several days. Even though some animals are
capable of acquiring some resistance to ticks after several infestations, tick attachment
to naïve cattle often result in skin lesions because host inflammatory reaction is not
enough to induce efficient responses capable of leading to ticks skins detachment.
In order to characterize the recruitment of local inflammatory responses, two
females were infested with laboratory reared Hyalomma lusitanicum
adult ticks. Ticks remained secured inside tissue sleeves, which were glued to the
bovine dorsum. Sampling occurred at different time points throughout the complete
period of infestation, from the attachment of unfed ticks to the detachment of fully
engorged (fertilized) females. Skin biopsies (8 mm) were fixed in tamponed
7%), embedded on paraffin blocks and later cut. Histological sections
m and stained by hematoxylin & eosin coloration. The microscopic observation
of the biopsies allowed skin lesions identification. Both superficial and deep dermatitis,
as well as interstitial and follicular, with diverse diffuse and perivascular patterns were
found. An infiltration with predominance of inflammatory cells (neutrophils and
eosinophil cells) was registered. Some lymphoid cells were identified as well as rare
In conclusion, significant differences were found in samples obtained from control
and the infested animal, as well as among those obtained in different phases of tick
engorgement allowing us to document different pathological changes due to this long
Meeting of the Portuguese Society of Animal Pathology Beyound Routine Diagnosis, 20 and 21 of May 2016
49
Hyalomma lusitanicum infestation
, Pereira da Fonseca I.1,
Centro de Investigação Interdisciplinar em Sanidade Animal (CIISA), Faculdade de
Estudos de Vetores e Doenças Infeciosas Dr. Francisco Cambournac,
Instituto Nacional de Saúde Dr. Ricardo Jorge I.P., Águas de Moura, Portugal.
animal and human pathogens. Hard ticks secure
their meals by attaching to a host for several days. Even though some animals are
capable of acquiring some resistance to ticks after several infestations, tick attachment
lesions because host inflammatory reaction is not
enough to induce efficient responses capable of leading to ticks skins detachment.
In order to characterize the recruitment of local inflammatory responses, two
Hyalomma lusitanicum
adult ticks. Ticks remained secured inside tissue sleeves, which were glued to the
bovine dorsum. Sampling occurred at different time points throughout the complete
icks to the detachment of fully
engorged (fertilized) females. Skin biopsies (8 mm) were fixed in tamponed
7%), embedded on paraffin blocks and later cut. Histological sections
roscopic observation
of the biopsies allowed skin lesions identification. Both superficial and deep dermatitis,
as well as interstitial and follicular, with diverse diffuse and perivascular patterns were
tory cells (neutrophils and
eosinophil cells) was registered. Some lymphoid cells were identified as well as rare
In conclusion, significant differences were found in samples obtained from control
those obtained in different phases of tick
engorgement allowing us to document different pathological changes due to this long
13. Identification of subcutaneous mite
Hypoderatidae) in the Northern gannet (
Gama A.1,2, Seixas F.1,2*
Mosteiró D.3, Coutinho T.1
1 Department of Veterinary Sciences, School of Agrarian and Veterinary Sciences,
University of Trás-os-Montes e Alto Douro (UTAD), Vila Real, Portugal2 Animal and Veterinary Research Centre (CECAV), UTAD, Vila Real, Portugal3 Centro de Recuperação de Animais Selvagens (CRAS), Hospital Veterinário da
UTAD, Vila Real, Portugal
*fseixas@utad.pt
An adult female Northern gannet (
northern Portugal, after swallowing two fishing hooks, but died shortly after emergency
surgery. At necropsy, a large number of perfec
were found in the subcutaneous tissue of the abdominal region, apparently without
associated inflammatory reaction. Those structures were identified as heteromorphic
deutonymphs (hypopi) of the subcutaneous mite
Hypoderidae), based on their size and additional morphological characteristics. The
mites were 1.38 mm in length and 0.57 mm in width on average, and presented distinct
typical coxal apodemes in the anterior part. To the best of o
first report of the subcutaneous mite
XXI Meeting of the Portuguese Society of Animal PathologyBeyound Routine Diagnosis, 20 and 21 of May 2016
Identification of subcutaneous mite Hypodectes propus
Hypoderatidae) in the Northern gannet (Morus bassanus) in Portugal1,2*, Pires M.A.1,2, Sargo R.3, Loureiro F.3, Sousa L
, Cardoso L.1, Lopes A.P.1,2
Department of Veterinary Sciences, School of Agrarian and Veterinary Sciences,
Montes e Alto Douro (UTAD), Vila Real, Portugal
Animal and Veterinary Research Centre (CECAV), UTAD, Vila Real, Portugal
Centro de Recuperação de Animais Selvagens (CRAS), Hospital Veterinário da
UTAD, Vila Real, Portugal
An adult female Northern gannet (Morus bassanus) was rescued in Esposende,
northern Portugal, after swallowing two fishing hooks, but died shortly after emergency
surgery. At necropsy, a large number of perfectly individualized cyst
were found in the subcutaneous tissue of the abdominal region, apparently without
associated inflammatory reaction. Those structures were identified as heteromorphic
deutonymphs (hypopi) of the subcutaneous mite Hypodectes propus
Hypoderidae), based on their size and additional morphological characteristics. The
mites were 1.38 mm in length and 0.57 mm in width on average, and presented distinct
typical coxal apodemes in the anterior part. To the best of our knowledge, this is the
first report of the subcutaneous mite H. propus in the Northern gannet in Portugal.
Meeting of the Portuguese Society of Animal Pathology Beyound Routine Diagnosis, 20 and 21 of May 2016
50
Hypodectes propus (Acarina:
) in Portugal
, Sousa L.3, Tomás J.3,
Department of Veterinary Sciences, School of Agrarian and Veterinary Sciences,
Montes e Alto Douro (UTAD), Vila Real, Portugal
Animal and Veterinary Research Centre (CECAV), UTAD, Vila Real, Portugal
Centro de Recuperação de Animais Selvagens (CRAS), Hospital Veterinário da
) was rescued in Esposende,
northern Portugal, after swallowing two fishing hooks, but died shortly after emergency
tly individualized cyst-like structures
were found in the subcutaneous tissue of the abdominal region, apparently without
associated inflammatory reaction. Those structures were identified as heteromorphic
dectes propus (Astigmata:
Hypoderidae), based on their size and additional morphological characteristics. The
mites were 1.38 mm in length and 0.57 mm in width on average, and presented distinct
ur knowledge, this is the
in the Northern gannet in Portugal.
14. Dirofilaria immitis in South African fur seal (Arctocephalus pusillus
pusillus): necropsy and histopathology findings observed in zoological context in
Portugal- Marcelino I.1,2*, FlanaganC.
Alho A.M.2
1Mundo Aquático S.A. –
Albufeira, Portugal. 2CIISA, Faculdade de Medicina Veterinária, ULisboa, Avenida da Universidade
Técnica, 1300-477 Lisboa, Portugal.
* ines.marcelino@campus.ul.pt
Dirofilariosis is an emerging zoonotic vector
companion and wild animals. Despite the importance of cardiopulmonary parasites,
studies conducted regarding
only in two species: California sea lion (
(Phoca vitulina).
A survey of cardiopulmonary parasites was performed in the collection of 19
pinnipeds belonging to Zoomarine
Algarve. In parallel, D. immitis
pulmonary artery in three necropsies of South African fur seals (
pusillus). Generalized congestion in the lu
Additionally, lesions of exudative pneumonia in red hepatization phase as well as,
interstitial pneumonia, emphysema, hemorrhagic lung lesions and also catarrhal
bronchitis were observed. The animals had lesions associated
parasite but not to a great extent. This was not the cause of death, but a number of
factors that were due to old age and immune status of the animals.
To the authors’ knowledge, this is the first worldwide report of
pusillus and the first report of this nematode and associated lesions in a pinniped
population from Portugal. Considering that
zoonotic parasite and extremely important in domestic and wild animal health, this work
will increase awareness of the disease in pinnipeds, so that veterinarians adopt control
measures against the vectors and perform preventive therapy in animals kept in
zoological contexts.
XXI Meeting of the Portuguese Society of Animal PathologyBeyound Routine Diagnosis, 20 and 21 of May 2016
Dirofilaria immitis in South African fur seal (Arctocephalus pusillus
pusillus): necropsy and histopathology findings observed in zoological context in
, FlanaganC.1, Silva N.1, Madeira de Carvalho L.
– Zoomarine – Est. Nacional 125, Cortelhas, Guia, 8201
CIISA, Faculdade de Medicina Veterinária, ULisboa, Avenida da Universidade
477 Lisboa, Portugal.
ines.marcelino@campus.ul.pt
Dirofilariosis is an emerging zoonotic vector-borne disease, with a major impact on
companion and wild animals. Despite the importance of cardiopulmonary parasites,
studies conducted regarding Dirofilaria immitis in pinnipeds are few, being reported
only in two species: California sea lion (Zalophus californianus) and common seal
A survey of cardiopulmonary parasites was performed in the collection of 19
pinnipeds belonging to Zoomarine – Mundo Aquático S.A., which is located in the
D. immitis adult nematodes were found in the right ventricle and
pulmonary artery in three necropsies of South African fur seals (Arctocephalus pusillus
). Generalized congestion in the lungs, liver and kidneys was noted.
Additionally, lesions of exudative pneumonia in red hepatization phase as well as,
interstitial pneumonia, emphysema, hemorrhagic lung lesions and also catarrhal
bronchitis were observed. The animals had lesions associated with the presence of the
parasite but not to a great extent. This was not the cause of death, but a number of
factors that were due to old age and immune status of the animals.
To the authors’ knowledge, this is the first worldwide report of D. immitis
and the first report of this nematode and associated lesions in a pinniped
population from Portugal. Considering that D. immitis is a worldwide emerging
zoonotic parasite and extremely important in domestic and wild animal health, this work
will increase awareness of the disease in pinnipeds, so that veterinarians adopt control
measures against the vectors and perform preventive therapy in animals kept in
Meeting of the Portuguese Society of Animal Pathology Beyound Routine Diagnosis, 20 and 21 of May 2016
51
Dirofilaria immitis in South African fur seal (Arctocephalus pusillus
pusillus): necropsy and histopathology findings observed in zoological context in
, Madeira de Carvalho L.2, Correia J.2,
Est. Nacional 125, Cortelhas, Guia, 8201-864
CIISA, Faculdade de Medicina Veterinária, ULisboa, Avenida da Universidade
borne disease, with a major impact on
companion and wild animals. Despite the importance of cardiopulmonary parasites,
in pinnipeds are few, being reported
) and common seal
A survey of cardiopulmonary parasites was performed in the collection of 19
quático S.A., which is located in the
adult nematodes were found in the right ventricle and
Arctocephalus pusillus
ngs, liver and kidneys was noted.
Additionally, lesions of exudative pneumonia in red hepatization phase as well as,
interstitial pneumonia, emphysema, hemorrhagic lung lesions and also catarrhal
with the presence of the
parasite but not to a great extent. This was not the cause of death, but a number of
D. immitis in A. p.
and the first report of this nematode and associated lesions in a pinniped
is a worldwide emerging
zoonotic parasite and extremely important in domestic and wild animal health, this work
will increase awareness of the disease in pinnipeds, so that veterinarians adopt control
measures against the vectors and perform preventive therapy in animals kept in
Occurrence of blood-sucking mites in ornamental chickens
Duque de Araújo A.M., Salvador B.M.C.
Faculdade de Medicina Veterinária, Universidade Lusófona, Campo Grande , 477
Lisboa, Portugal.
* araujoam2@gmail.com
Domestic and ornamental birds can be parasitized by hematophagous Dermanyssoid
mites belonging to Dermanyssidae and Macronyssidae families. They are responsible
for decrease of productivity, can be vectors of diseases and interfere with bird’s welfare,
and other hosts including humans can be parasitized, being responsible for skin
problems and transmission of zoonoses. Morphologically,
closely resembles Ornithonyssus sylviarum
economic losses in the poultry industry but they differ in their life cycles. Nymphs and
adults of D. gallinae remain on the hosts to feed, spending most of his life cycles in the
cracks and crevices of the bird’s facilities.
On March of 2016 was observed a high infestation by mi
from a farm located in center of Portugal. The birds had weight loss, intense itching,
and erythematous dermatitis, depluming, behavioral changes and decrease of egg
production. Infestation by Dermanyssoid mites was observed in 1
Old English breeds and in the nests of Japan Silky. Mites were collected directly from
the body of birds and in the facilities. The diagnosis was made by optical microscopy.
Specimens collected have been identified as “Dermanyssoid m
difficulty in identifying D. gallinae
observed presence of eggs, larvae, nymphs and adults and in the nests was observed
mostly adults.
Treatment of birds was carried out individually w
fipronil (Frontline®), disinfestation of facilities and nests through a flamethrower ("fire
broom") and placement of diatomaceous earth in bathing places of birds.
After two weeks of daily observation of birds and facilities
The improvement of general condition and eggs production was regularized.
XXI Meeting of the Portuguese Society of Animal PathologyBeyound Routine Diagnosis, 20 and 21 of May 2016
sucking mites in ornamental chickens
Araújo A.M., Salvador B.M.C.
Faculdade de Medicina Veterinária, Universidade Lusófona, Campo Grande , 477
araujoam2@gmail.com
Domestic and ornamental birds can be parasitized by hematophagous Dermanyssoid
mites belonging to Dermanyssidae and Macronyssidae families. They are responsible
for decrease of productivity, can be vectors of diseases and interfere with bird’s welfare,
other hosts including humans can be parasitized, being responsible for skin
problems and transmission of zoonoses. Morphologically, Dermanyssus gallinae
Ornithonyssus sylviarum, both are responsible for numerous
ultry industry but they differ in their life cycles. Nymphs and
remain on the hosts to feed, spending most of his life cycles in the
cracks and crevices of the bird’s facilities.
On March of 2016 was observed a high infestation by mites in ornamental chickens
from a farm located in center of Portugal. The birds had weight loss, intense itching,
and erythematous dermatitis, depluming, behavioral changes and decrease of egg
production. Infestation by Dermanyssoid mites was observed in 10 birds of Paduana and
Old English breeds and in the nests of Japan Silky. Mites were collected directly from
the body of birds and in the facilities. The diagnosis was made by optical microscopy.
Specimens collected have been identified as “Dermanyssoid mites" because there was
D. gallinae and O. sylviarum. On the body of the birds was
observed presence of eggs, larvae, nymphs and adults and in the nests was observed
Treatment of birds was carried out individually with the topical application of
fipronil (Frontline®), disinfestation of facilities and nests through a flamethrower ("fire
broom") and placement of diatomaceous earth in bathing places of birds.
After two weeks of daily observation of birds and facilities no mites was observed.
The improvement of general condition and eggs production was regularized.
Meeting of the Portuguese Society of Animal Pathology Beyound Routine Diagnosis, 20 and 21 of May 2016
52
Faculdade de Medicina Veterinária, Universidade Lusófona, Campo Grande , 477
Domestic and ornamental birds can be parasitized by hematophagous Dermanyssoid
mites belonging to Dermanyssidae and Macronyssidae families. They are responsible
for decrease of productivity, can be vectors of diseases and interfere with bird’s welfare,
other hosts including humans can be parasitized, being responsible for skin
Dermanyssus gallinae
, both are responsible for numerous
ultry industry but they differ in their life cycles. Nymphs and
remain on the hosts to feed, spending most of his life cycles in the
tes in ornamental chickens
from a farm located in center of Portugal. The birds had weight loss, intense itching,
and erythematous dermatitis, depluming, behavioral changes and decrease of egg
0 birds of Paduana and
Old English breeds and in the nests of Japan Silky. Mites were collected directly from
the body of birds and in the facilities. The diagnosis was made by optical microscopy.
ites" because there was
. On the body of the birds was
observed presence of eggs, larvae, nymphs and adults and in the nests was observed
ith the topical application of
fipronil (Frontline®), disinfestation of facilities and nests through a flamethrower ("fire
broom") and placement of diatomaceous earth in bathing places of birds.
no mites was observed.
The improvement of general condition and eggs production was regularized.
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