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0.00E+00
5.00E+08
1.00E+09
1.50E+09
2.00E+09
2.50E+09
3.00E+09
3.50E+09
4.00E+09
4.50E+09
Normal BPH AggressivePCa
A
Size (nm)
Con
cent
ratio
n (E
6 pa
rticl
es/m
l)
100 200 300 400 500 600 700 800 900 1000
149.75BPH Aggressive PCa
Size (nm)
Con
cent
ratio
n (E
6 pa
rticl
es/m
l)
100 200 300 400 500 600 700 800 900 1000
128.27
CD63
TSG101
BPH Aggressive PCa
CB
0
20
40
60
80
100
120
Normal BPH AggressivePCa
Con
cent
ratio
n (E
6 pa
rticl
es/m
l)
Normal382.21
100 200 300 400 500 600 700 800 900 1000
Con
cent
ratio
n (E
6 pa
rticl
es/m
l)
Size (nm)
Fig. S1 Characterization of serum exosomal preparations
P=0.109
Siz
e (n
m)
P=0.376 P=0.472
P=0.216
BP
H1
BP
H2
BP
H3
PC
a1P
Ca2
PC
a3P
Ca4
PC
a5P
Ca6
D
024681012141618
11.2
11.3
11.4
11.5
11.6
11.7
11.8
ErbBreceptorsignalingnetwork
Plasmamembraneestrogenreceptorsignaling
Nectinadhesionpathway
IGF1pathway
LKB1signalingevents
Signalingevents
mediatedby
HepatocyteGrowthFactor
Receptor(c-Met)
TRAILsignalingpathway
IFN-gammapathway
-log1
0(p)
Perc
enta
ge o
f gen
es
Biological pathway
Percentage of genes p-value p=0.05 reference
Fig. S2 Biological pathways potentially influenced by dysregulated miRNAs in aggressive PCa as compared to BPH
A
0.001
0.01
0.1
1
10
100
1000 Exosomal Circulating
Rel
ativ
e ex
-miR
-124
6 ex
pres
sion
Samples
0
5
10
15
20
25
30
35
0 50 100 150 200 250
Circ
ulat
ing
miR
-124
6 ex
pres
sion
Exosomal miR-1246 expression
Correlation coefficient r: -0.157195% CI: -0.4897 to 0.2153P-value: 0.4070 (ns)
Fig. S3 Exosomal miR-1246 expression patterns differ from circulating miR-1246 expresssion in sera of prostate cancer patients
B
0
2
4
6
8
10
12
Cohort 2 Cohort 1 Cohort 3
Rel
ativ
e ex
-miR
-124
6 ex
pres
sion
Fig. S4 ex-miR-1246 expression as a measure of PCa aggressiveness
(Localized mets)
(Distant mets)
(No mets)
Aggressiveness
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