CBE654 Introductory Lecture (Current Tech. and Infos) 6macEND

Bioreactor Engineering

CBE654EH222 6A & 6B

Introductory lecture on Bioreactors(current technology and informations)

Amizon Azizan

Faculty of Chemical Engineering

Lesson Plan

• Lesson plan– CBE654_BIOREACTOR_ENGINEERING_LE

SSON_PLAN_5Mac.doc

• Introduction– Amizon– Students

Bioreactor Engineering

• Introductions– Applications– Examples of bioreactors

• Stirred (bioreactor/fermenter)– Infors, Sartorius etc

» http://www.infors-ht.com/index.php/en/» http://www.sartorius-stedim.com/WW/en/fermentation_and_cell_c

ultivation/6ehw52oyd0c_artikel_liste.htm» Examples of autoclavable bioreactor, in situ sterilizable bioreactor,

single use bioreactor (Sartorius)

• Microbioreactor (Biolector, RAMOS, BCpreFerm etc)

Bioreactor Engineering

• Industrial scale bioreactor• Malaysian scenario• http://www.chemicals-technology.com/projects/sirim-bioplastics-pilot-plant/sirim-bi

oplastics-pilot-plant3.html• The Malaysian designed SIRIM Bioreactor Technology at the pilot plant has

integrated production processes and bioreactor facilities producing 2,000l of different PHA materials. Image courtesy of SIRIM Berhad.

• Polyhydroxyalkanoates or PHAs are linear polyesters produced in nature by bacterial fermentation of sugar or lipids. They are produced by the bacteria to store carbon and energy. More than 150 different monomers can be combined within this family to give materials with extremely different properties.[1] These plastics are biodegradeable and are used in the production of bioplastics.

• Contact person• Adida Zuraida, SIRIM Incubation Centre, Lot 10, Kawasan MIEL, Fasa 2, Jalan

Beremban 15/12, 40000 Shah Alam, Selangor• SIRIM Bioplastics Pilot Plant WORD.docx

• Alpha Biologics• alpha biologics.docx

Alpha Biologics

There is a buffer preparation unit with 50l, 120l, 250l and 1000l preparation vessels.

The bioreactors range from 10 to 500l capacity.

The facility has seven bioreactors.

Bioreactors can be operated in batch, fed-batch or perfusion mode.

Bioreactor Engineering

• Industrial scale bioreactor

• World scenario– Bioethanol plant

• Brochure5_Bioethanol_low_res.pdf• Production of bioethanol in the UE.docx

Bioreactor-fermentation

Bioreactors

• Autoclavable bioreactors

• Flyer-Minifors-(en).pdf

• Flyer-Labfors-5-(en).pdf

Bioreactor

Bioreactor

Other schematic diagram of a reactor

Bioreactor

• In situ sterilizable bioreactors

• Flyer-Techfors-(en).pdf

• Broch_BIOSTATCplus_SBI1505-e_pdf.pdf

Bioreactors

• Single use bioreactors– http://www.sartorius-stedim.com/WW/en/

Single-use-Bioreactors/9rdq4s5bimw_artikel_liste.htm

– Broch_BIOSTATCultiBagRMcomp_SBT1501-e.pdf

– Broch_BIOSTAT_Standard_Systems_Overview_SBI1511-e.pdf

– Single use technology Xcellerex.pdf

FermentationPrinciples_of_Fermentation_Technology.pdf

Bioreactor design

Bioreactor design:

Page 179

Suitable for high viscosity broth

Agitator for gas-phase mixing, air dispersion, oxygen transfer, heat transfer, suspension of solid particles.

Bioreactor : agitator

Bioreactor : agitator