Chemistry. Session Session Objectives 1.Enzymes 2.Cofactors 3.Sucrase Mechanism 4.Carboxypeptidase...

Chemistry

Session

Session Objectives

1. Enzymes

2. Cofactors

3. Sucrase Mechanism

4. Carboxypeptidase

5. Metabolism

6. DNA and RNA

7. Lipids

8. Hormones and Vitamins

Enzymes

An enzyme is a protein that acts as a catalyst for a biological reaction.

Most enzymes are specific for substrates while enzymes involved in digestion such as papain attack many substrates

Cofactors

In addition to the protein part, many enzymes also have a nonprotein part called a cofactor

The protein part in such an enzyme is called an apoenzyme, and the combination of apoenzyme plus cofactor is called a holoenzyme. Only holoenzymes have biological activity; neither cofactor nor apoenzyme can catalyze reactions by themselves

A cofactor can be either an inorganic ion or an organic molecule, called a coenzyme

Many coenzymes are derived from vitamins, organic molecules that are dietary requirements for metabolism and/or growth

Types of Enzymes by Function

Enzymes are usually grouped according to the kind of reaction they catalyze, not by their structures

How Do Enzymes Work? Citrate Synthase

Citrate synthase catalyzes a mixed Claisen condensation of acetyl CoA and oxaloacetate to give citrate

Normally Claisen condensation require a strong base in an alcohol solvent but citrate synthetase operates in neutral solution

Sucrase Mechanism

→

Sucrase Mechanism

→→

Active Site of Carboxypeptidase

Carboxypeptidase's

The Structure of Citrate Synthase

Determined by X-ray crystallography

Enzyme is very large compared to substrates, creating a complete environment for the reaction

Aspects of Metabolism

Metabolism.

The life process.

–Catabolism.

• Substances are broken down.

–Anabolism.

• Substances are built up.

Metabolism

• Lipid metabolism.

–Uptake of fats through walls of intestine.

–Glycerol converted to glyceraldehyde-3-phosphate.

–Fatty acids are oxidized by –oxidation.

• Protein metabolism.

–Stomach:

• HCl and pepsin hydrolize 10% of peptide bonds.

–Intestine:

• Trypsin and chymotrypsin cleave peptide fragments further.

Components of DNA and RNADNA: Deoxyribonucleic acid.

Chromosomes: Double stranded DNA rod-like structures.

Genes: Specific locations on chromasomes that code for specific traits.

RNA: Ribonucleic acid

Contains ribose instead of deoxyribose.

Generally single stranded.

DNA Double Helix

RNA Single Strand

Focus On Protein Synthesis and the Genetic Code

The genetic code

How triplets of the four nucleotides unambiguously specify 20 amino acids, making it possible to translate information from a nucleotide chain to a sequence of amino acids.

Transcription

How RNA polymerase, guided by base

pairing, synthesizes a single-stranded

mRNA copy of a gene’s DNA template

Protein Synthesis(Translation)

Translation

How base pairing between mRNA and tRNAs directs the assembly of a polypeptide on the ribosome

DNA Replication

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Lipids

Some Common Fatty Acids

Saponification

Fats and Oils

Both are triglycerides.

–Differ in the nature of the acid components attached.

– Both are colorless, odorless and tasteless

– Flavors and aromas come from organic impurites.

Fats.

–Predominantly saturated fatty acids.

–Normally solid at room temperature

Oils.

–Predominantly unsaturated fatty acids.

–Liquids at room temperature.

Butter

“Calorie-Free” Fats

Phospholipids

Phospholipids

Hormones

Hormones are molecules that transfer information from one group of cells to a distant tissue or organ.

They are produced by various endocrine glands.

They are classified on the basis of their structure or site of activity in the cell.

Adrenal cortical horm ones(corticodes)

Estrogen Progestrone

Fem ale sex hom ones Male sex horm ones(Androgens)

Sex horm ones

Stero ids

Peptide horm ones(insulin , g lucagon)

Am ino acid derivatives(thyroidal hormones)

Miscellaneous(Prostaglandins, cyokin ins)

Non-Stero ids

Hormones

Vitamins

They are essential dietary factors required by an organism in minute quantities.

They are essential for life and their absence causes deficiency diseases.

Vitamins catalyze biological reactions in very low concentration

Vitamins are designated A,B,C,D,etc. in order of their discovery. Subgroup vitamins are designated by number subscript e.g. B1,B2,B6,B12

Classification:A.Fat solubleB.Water soluble

Thank you