View
219
Download
0
Category
Preview:
Citation preview
Disease Gene Candidate Prioritization by Integrative Biology
Table of contents:
Where are we in the course pipeline?
Maturation of the project and the project description
Background
Networks – deducing functional relationships from PPI data networksProtein interaction networksFunctional modules / network clusters
Phenotype associationGrouping disorders based on their phenotype.Biological implications of phenotype clusters.
Method and examplesIntegrating protein interaction data and phenotype associations in an automatedlarge scale disease gene finding platform
Sample PreparationHybridization
Array designProbe design
QuestionExperimental Design
Buy Chip/Array
Statistical AnalysisFit to Model (time series)
Expression IndexCalculation
Advanced Data AnalysisClustering PCA Classification Promoter AnalysisMeta analysis Survival analysis Regulatory Network
ComparableGene Expression Data
Normalization
Image analysis
The DNA Array Analysis Pipeline
Project description
Søren Brunak, Professor, center director, Dr. Phil, PhD
Niels Tommerup Professor, Centre Director, Dr. Med.
Masters thesis sept 2003
Project description
Project:
Søren Brunak, Professor, center director, Dr. Phil, PhD
Niels Tommerup Professor, Centre Director Dr. Med.
Find disease genes
Using Bioinformatics
Project description
Project:
Søren Brunak, Professor, center director, Dr. Phil, PhD
Niels Tommerup Professor, Centre Director Dr. Med.
Disease gene candidate prioritization by integrating protein interaction and phenotype association data.
Project descriptionAbstractThe availability of the first draft of the human genome in 2001 (Venter, Adams et al. 2001) led to an increase in the number of methods for disease gene identification. However, the general number of candidates in most loci linked to a particular phenotype is in the hundreds (McCarthy, Smedley et al. 2003; van Driel, Cuelenaere et al. 2003), and the underlying genes in over 900 of the ~ 2550 loci associated with a phenotype in the “Online Mendelian Inheritance in Man” (OMIM) database, have not yet been identified (Hamosh, Scott et al. 2005). Evidently disease gene identification continues to be a very strenuous challenge, since mutational analysis of hundreds of candidates in a critical interval using methods currently available is extremely resource demanding. Thus, prioritising the candidates based on different criteria followed by an extensive investigation of promising candidates, is a logical step in the disease gene finding process. With the advent of proteomics, we are now able to retrieve information on gene functions in a large-scale manner, thus bridging the gap between genotype and phenotype, a possibility with significant interest for disease gene candidate prioritization.We propose that automated correlation of phenotype association networks, with interolog data (the transfer of protein interactions between orthologous protein pairs in different organisms), is a powerful way of identifying good disease gene candidates in a large list of genes in loci associated with a phenotype. Our method automatically identifies potential functional modules consisting of protein components, where at least one of the components is a disease related protein. When such incriminated modules are identified, the remaining protein components of the module are correlated with loci in the genome associated with a similar phenotype. A hit is reported if other protein components of the incriminated module are the product of genes in loci associated with an identical or overlapping phenotype. Using this large scale approach we show that a gene in a locus is a heavily incriminated candidate, if the protein product of the gene interacts with a protein involved in a similar or identical phenotype, and publish a list of 60 likely candidates in various disorders.
Project descriptionAbstractThe availability of the first draft of the human genome in 2001 (Venter, Adams et al. 2001) led to anincrease in the number of methods for disease gene identification. However, the general number of candidates in most loci linked to a particular phenotype is in the hundreds (McCarthy, Smedley et al. 2003; van Driel, Cuelenaere et al. 2003), and the underlying genes in over 900 of the ~ 2550 loci associated with a phenotype in the “Online Mendelian Inheritance in Man” (OMIM) database, have not yet been identified (Hamosh, Scott et al. 2005). Evidently disease gene identification continues to be a very strenuous challenge, since mutational analysis of hundreds of candidates in a critical interval using methods currently available is extremely resource demanding. Thus, prioritising the candidates based on different criteria followed by an extensive investigation of promising candidates, is a logical step in the disease gene finding process. With the advent of proteomics, we are now able to retrieve information on gene functions in a large-scale manner, thus bridging the gap between genotype and phenotype, a possibility with significant interest for disease gene candidate prioritization.We propose that automated correlation of phenotype association networks, with interolog data (the transfer of protein interactions between orthologous protein pairs in different organisms), is a powerful way of identifying good disease gene candidates in a large list of genes in loci associated with a phenotype. Our method automatically identifies potential functional modules consisting of protein components, where at least one of the components is a disease related protein. When such incriminated modules are identified, the remaining protein components of the module are correlated with loci in the genome associated with a similar phenotype. A hit is reported if other protein components of the incriminated module are the product of genes in loci associated with an identical or overlapping phenotype. Using this large scale approach we show that a gene in a locus is a heavily incriminated candidate, if the protein product of the gene interacts with a protein involved in a similar or identical phenotype, and publish a list of 60 likely candidates in various disorders.
Background
Background
Finding genes responsible for major genetic disorders can lead to diagnostics, potential drug targets, treatments and large amounts ofinformation about molecular cell biology in general.
BackgroundMethods for disease gene finding post genome era (>2001):
Mircodeletions Translocations
http://www.med.cmu.ac.th/dept/pediatrics/06-interest-cases/ic-39/case39.html
http://www.rscbayarea.com/images/reciprocal_translocation.gif
Linkage analysis
Fagerheim et al 1996.
1q21-1q23.1
chr1:141,600,00-155,900,000
BackgroundAutomated methods for disease gene finding int the post genome era (>2001):
?
(Perez-Iratxeta, Bork et al. 2002) (Freudenberg and Propping 2002)(van Driel, Cuelenaere et al. 2005)(Hristovski, Peterlin et al. 2005)
Grouping:
Tissues, Gene Ontology, Gene Expression, MeSH terms …….
Disease Gene Finding.
Summery
Background
Why do we want to find disease genes, how has it been done until now?
Networks – deducing functional relationships from network theory
Protein interactionnetworksFunctional modules / network clusters
Phenotype association
Grouping disorders based on their phenotype.Biological implications of phenotype clusters.
Method and examples
Combining network theory and phenotype associationsin an automated large scale disease gene finding platformproof of concept.Status of pipeline / infrastructure
Networks and functional modulesDeducing functional relationships from protein interaction networks
Networks and functional modulesDeducing functional relationships from network theory
Network theory is boooooooooring
Networks
Text mining of full text corpora e.g PubMed Central
http://www.biosolveit.de/ToPNet/screenshots/fig1.html
Protein interaction networks of physical interactions.
(Barabasi and Oltvai 2004).
Networks
daily
weekly
monthly
(de Licthenberg et al.)
Social Networks, The CBS interactome
Networks
daily
weekly
monthly
(de Licthenberg et al.)
Social Networks, The CBS interactome
Networks
Extracting functional data from protein interactionnetworks
InWeb
Homo Sapiens
The Ach receptor involved in MyasthenicSyndrome.
Dynamicfuncionalmodule:
Eg:
Cell cycleregulationMetabolism
Trans-organism protein interaction network
Orthologs?Orthologous genes are direct descendants of a gene in a commonancestor:
(O'Brien K, Remm et al. 2005)
S.Cerevisiae
D. Melanogaster
H.Sapiens
D. Melanogaster Experim.
C. Elegans Experim.
S. Cerevisiae Experim.
H.Sapiens MOSAIC
Trans-organism protein interaction network
Infrastructure status
BIND
IntAct
DIP
MINT
HPRD
Hand-curated
sets
PPI –pred.
GRID
InWeb
Homo Sapiens
Trans-organism ppi pipeline
>122.000 int.
> 22.000 genes
Scoring
A) Topological
B) No publ.
Extractionperl modules
Direct SQL access
XML or SIF output
Web serverOpis
Command lineInweb.pl
CBS DatawarehouseDownload/reformat db’s
Disease Gene Finding.
Summery
Background
Why do we want to find disease genes, how has it been done until now?
Networks – deducing functional relationships from network theory
Protein interactionnetworksFunctional modules / network clusters
Phenotype association
Grouping disorders based on their phenotype.Biological implications of phenotype clusters.
Method and examples
Combining network theory and phenotype associationsin an automated large scale disease gene finding platformproof of concept.Status of pipeline / infrastructure
Phenotype association
Phenotype association
Absent liver peroxisomesHepatomegalyIntrahepatic biliary dysgenesisProlonged neonatal jaundicePyloric hypertrophyPatent ductus arteriosusVentricular septal defectsBell-shaped thoraxSmall adrenal glandsAbsent renal peroxisomesClitoromegalyCryptorchidismHydronephrosisHypospadiasRenal cortical microcystsFailure to thriveAbnormal electroretinogramAbnormal helicesAnteverted naresBrushfield spotsCataractsCorneal clouding
Epicanthal foldsFlat faciesFlat occiputGlaucomaHigh arched palateHigh foreheadHypertelorismLarge fontanellesMacrocephalyMicrognathiaNystagmusPale optic diskPigmentary retinopathyPosteriorly rotated earsProtruding tongueRedundant skin folds of neckRound faciesSensorineural deafnessTurribrachycephalyUpward slanting Hyporeflexia or areflexiaHypotonia
Zelwegger syndrome
PolymicrogyriaSeizuresSevere mental retardationSubependymal cystsPulmonary hypoplasiaCubitus valgusDelayed bone ageMetatarsus adductusRocker-bottom feetStippled epiphyses (especially patellar and acetabular regions)Talipes equinovarusTransverse palmarcreaseUlnar deviation of handsWide cranial suturesTransverse palmarcreaseHeterotopias/abnormal migrationHypoplastic olfactory lobes
palpebral fissuresAutosomal recessiveAlbuminuriaAminoaciduriaDecreased dihydroxyacetonephosphate acyltransferase (DHAP-AT) activityDecreased plasmologenElevated long chain fatty acidsElevated serum iron and iron binding capacityIncreased phytanic acidPipecolic acidemiaBreech presentationDeath usually in first year of lifeGenetic heterogeneityInfants occasionally mistaken as having Down syndromeAgenesis/hypoplasic corpus collosum
Phenotype association
Word vectors
Phenotype Sim. Score
Adrenoleukodystrophy (202370) 0.781Hyperpipecolatemia (239400) 0.703Cerebrohepatorenal Syndr. (214110) 0.682Refsum Disease (266510) 0.609
Reference : Zelwegger Syndrome (214100)
A relationship between the infantile form of Refsumdisease and Zellweger syndrome was suggested by the observations of Poulos et al. (1984) in 2 patients. In the infantile form of Refsum disease, as in Zellwegersyndrome, peroxisomes are deficient and peroxisomalfunctions are impaired (Schram et al., 1986). Clinically, infantile Refsum disease, ZWS, and adreno-leukodystrophy have several overlapping features. (Stokke et al., 1984).(http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=266510)
214100 202370
Phenotype association
Word vectorsPhenotype association network
Cerebro-Hepato-
renalZelwegger
Refsum
Adrenoleuko-dystrophy
Disease Gene Finding.
Summery
Background
Why do we want to find disease genes, how has it been done until now?
Networks – deducing functional relationships from network theory
Protein interactionnetworksFunctional modules / network clusters
Phenotype association
Grouping disorders based on their phenotype.Biological implications of phenotype clusters.
Method and examples
Combining network theory and phenotype associationsin an automated large scale disease gene finding platformproof of concept.
Method –
Status
Method
InWeb
Homo Sapiens
Word vectors
Phenotype clustering
Method - statusIn Vitro:
Blinded tests on 15 linkage intervals with one novel angiogenesis gene per interval.
12 predictions, 7 correct, 2 novel.
In patient material:
Usher
Septooptic dysplasia syndrome
Dilated cardiomyopathy
Benchmarking:
Benchmarking by unbiased prioritizing genes in ~ 1404 critical intervals where the actual disease gene is known.
How well does it work ?
How well does the method work ?
Is it unbiased ?
Reveals novel global aspect ofhuman diseases
Results - BenchmarkMIM RANK GENE P-value TRUE
278800 1 ENSG00000032514 0.300326793109544 *278800 2 ENSG00000188611 0.0125655342047565278800 2 ENSG00000138297 0.0125655342047565278800 2 ENSG00000165406 0.0125655342047565278800 3 ENSG00000196693 0.0121357313793756278800 3 ENSG00000185532 0.0121357313793756278800 4 ENSG00000197910 0.00680983722337082278800 4 ENSG00000165383 0.00680983722337082278800 4 ENSG00000172538 0.00680983722337082. . . .. . . .. . . .. . . .. . . .278800 4 ENSG00000165511 0.00680983722337082278800 4 ENSG00000182354 0.00680983722337082278800 4 ENSG00000172661 0.00680983722337082278800 4 ENSG00000165507 0.00680983722337082278800 4 ENSG00000178440 0.00680983722337082278800 4 ENSG00000138299 0.00680983722337082278800 4 ENSG00000197704 0.00680983722337082278800 4 ENSG00000012779 0.00680983722337082278800 4 ENSG00000197354 0.00680983722337082278800 4 ENSG00000189090 0.00680983722337082278800 4 ENSG00000107551 0.00680983722337082278800 4 ENSG00000126542 0.00680983722337082278800 4 ENSG00000198364 0.00680983722337082278800 4 ENSG00000185849 0.00680983722337082278800 4 ENSG00000150165 0.00680983722337082278800 4 ENSG00000128815 0.00680983722337082278800 4 ENSG00000178645 0.00680983722337082278800 4 ENSG00000138293 0.00680983722337082278800 4 ENSG00000176833 0.00680983722337082278800 4 ENSG00000179251 0.00680983722337082278800 4 ENSG00000169826 0.00680983722337082278800 4 ENSG00000172678 0.00680983722337082278800 4 ENSG00000197752 0.00680983722337082278800 5 ENSG00000107643 0.00412573091718715278800 6 ENSG00000165733 0.000263885640603109
278800 7 ENSG00000169813 6,63E+07
DE SANCTIS-CACCHIONE SYNDROME
Gene map locus 10q11 >12MB area, 103 ranked genes
CLINICAL FEATURES
De Sanctis and Cacchione (1932) reported a condition, which theycalled 'xerodermic idiocy,' in whichpatients had xerodermapigmentosum, mental deficiency, progressive neurologicdeterioration, dwarfism, and gonadal hypoplasia.http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=278800
Results – Benchmarking
DE SANCTIS-CACCHIONE SYNDROME
Ranked 1 P-value 0.300326793109544
DNA excision repair
protein ERCC-6
Eukaryotic translation initiation factor 4E (eIF4E)
DNA excision repair protein ERCC-2
Eukaryotic initiation factor 4A-I (eIF4A-I)
*126340 DNA REPAIR DEFECT EM9 OF CHINESE HAMSTER OVARY CELLS, COMPLEMENTATION OF; EM9
#133540 COCKAYNE SYNDROME CKN2
#278730 XERODERMA PIGMENTOSUM, COMPLEMENTATION GROUP D
#278800 DE SANCTIS-CACCHIONE SYNDROME
#601675 TRICHOTHIODYSTROPHY
Results – Benchmarking
DE SANCTIS-CACCHIONE SYNDROME
Ranked 2 P-value 0.0125655342047565
Disease Gene Finding.
Summery
Background
Why do we want to find disease genes, how has it been done until now?
Networks – deducing functional relationships from network theory
Protein interactionnetworksFunctional modules / network clusters
Phenotype association
Grouping disorders based on their phenotype.Biological implications of phenotype clusters.
Method and examples
Combining network theory and phenotype associationsin an automated large scale disease gene finding platformproof of concept.
Project descriptionAbstractThe availability of the first draft of the human genome in 2001 (Venter, Adams et al. 2001) led to anincrease in the number of methods for disease gene identification. However, the general number of candidates in most loci linked to a particular phenotype is in the hundreds (McCarthy, Smedley et al. 2003; van Driel, Cuelenaere et al. 2003), and the underlying genes in over 900 of the ~ 2550 loci associated with a phenotype in the “Online Mendelian Inheritance in Man” (OMIM) database, have not yet been identified (Hamosh, Scott et al. 2005). Evidently disease gene identification continues to be a very strenuous challenge, since mutational analysis of hundreds of candidates in a critical interval using methods currently available is extremely resource demanding. Thus, prioritising the candidates based on different criteria followed by an extensive investigation of promising candidates, is a logical step in the disease gene finding process. With the advent of proteomics, we are now able to retrieve information on gene functions in a large-scale manner, thus bridging the gap between genotype and phenotype, a possibility with significant interest for disease gene candidate prioritization.We propose that automated correlation of phenotype association networks, with interolog data (the transfer of protein interactions between orthologous protein pairs in different organisms), is a powerful way of identifying good disease gene candidates in a large list of genes in loci associated with a phenotype. Our method automatically identifies potential functional modules consisting of protein components, where at least one of the components is a disease related protein. When such incriminated modules are identified, the remaining protein components of the module are correlated with loci in the genome associated with a similar phenotype. A hit is reported if other protein components of the incriminated module are the product of genes in loci associated with an identical or overlapping phenotype. Using this large scale approach we show that a gene in a locus is a heavily incriminated candidate, if the protein product of the gene interacts with a protein involved in a similar or identical phenotype, and publish a list of 60 likely candidates in various disorders.
Project description
Project:
Søren Brunak, Professor, center director, Dr. Phil, PhD, physicist
Niels Tommerup Professor, Centre Director Dr. Med.
Find disease genes
Using Bioinformatics
Acknowledgments
Disease Gene Finding :
Olga RiginaOlof Karlberg
Zenia M. Størling Páll Ísólfur Ólason
Kasper LageAnders Gorm
Anders HinsbyYves MoreauSøren Brunak
Recommended