Fish DNA Barcoding Game!
A Jeopardy-Style Quiz Game
Jeopardy is a trademark of Jeopardy Productions, Inc.
Answer
An organelle other than the
nucleus that contains DNA.
Question
What is a mitochondrion?
Answer
All of the chromosomalDNA found in a cell.
Question
What is genomic DNA?
Answer
The process of rupturinga cell to release its
contents.
Question
What is lysis?
Answer
A suspension that trapsimpurities from fish tissue
and prevents contamination
of DNA.
Question
What is matrix?
Answer
The liquid portion above the pellet
formed after centrifugation.
Question
What is the supernatant?
Answer
Of the four PCR reactionsin your table setup today,
this is the sample thatdoesn’t have any DNA in
it.
Question
What is the negative control?
DAILY
How much do you wish to wager?
DOUBLE
Daily DoubleAnswer
An enzyme used in PCRthat is able to function atvery high temperatures.
Daily DoubleQuestion
What is Taq polymerase?
Answer
These target the portionof DNA to be amplified.
Question
What are primers?
Answer
A step in PCR in which thetemperature is lowered to
50–65ºC, allowing the primers to bind to the
DNA.
Question
What is annealing?
Answer
The complementary strandof the ACTGGTTA templatestrand that is being copied
during extension.
Question
What is TGACCAAT?
Answer
The name of the genethat is being amplified
by PCR in the Fish Barcoding Kit.
Question
What is COI?
Answer
This acts as a sieve toseparate DNA based on
its size when an electricalfield is applied.
Question
What is an agarose gel?
Answer
The size of the amplifiedportion of the COI gene.
Question
What is 650 bp?
Answer
It weighs down theDNA sample so that it
staysin the well after loading.
Question
What is loading dye?
Answer
It’s used to determine the size
of the DNA (in base pairs) in an agarose gel.
Question
What is a DNA ladder ormolecular weight ruler?
Answer
Where negatively chargedDNA migrates to during
gel electrophoresis.
Question
What is the positive oranode side of a gel
electrophoresis chamber?
Answer
P1000P200P50P20
Question
Which of these isnot an actual pipet?
Answer
DNA extractionRNA extraction
PCR Gel electrophoresis
Question
What is RNA extraction?(not part of today’s experiment)
Answer
CodTrout
CatfishMussel
Question
What is mussel?(not likely to work with fish barcoding kit)
Answer
DenaturationAnnealingExtension
Sequencing
Question
What is sequencing?(not a step in PCR)
Answer
Thermal cyclerCathodeAnode
Electrical current
Question
What is a thermal cycler?(not used in gel electrophoresis)
Answer
The identification of anorganism at the species
levelusing a several hundred
base pair sequence of DNA.
Question
What is barcoding?
Answer
The order of bases thatmake up a DNA strand.
Question
What is a sequence?
Answer
The database used toidentify a mystery fishat the species level.
Question
What is BOLD?
Answer
A group of organismscapable of interbreeding
andproducing fertile offspring.
Question
What is a species?
Answer
A place where specimensare stored to support
futurescientific investigations.
Question
What is a biorepository?