View
229
Download
0
Category
Preview:
Citation preview
GreenTechnologies GmbH
plantdustrial – linking plant and industrial biotechnology
Dr. Peter WeltersPhytowelt GreenTechnologies GmbH
ABIC2008, Cork, 26.08.08
GreenTechnologies GmbH
Monococcum Mint protoplasts Seedless Tomatoes
Biological Conventional GM-Agriculture Agriculture Agriculture
Optimization of Plants
GreenTechnologies GmbH
Life on Earth has evolved from a mélange ofchemicals via microbial organisms and
algae towards plants!
Why should chemistry be different?
Evolution
GreenTechnologies GmbH
A B
Renewable Resources
GreenTechnologies GmbHSustainable Chemistry
2005: $ bio 77 2010: $ bio 125
McKinsey 2006
0
5
10
15
20
25
30
35
40
45
$ bio
BioFue
ls
Plant E
xtrac
ts
Pharm
aIngre
dients
BulkChe
mica
ls&Poly
mers
Food
/Feed
Ingre
dients
Oleoch
emica
ls
Enzym
es
Others
GreenTechnologies GmbH
GMOs
Mol. Farm.
BioEnergy
Fermentation withMicrobial Cells
Fermentation improved byPlant Enzymes
Fermentation replacedby Plant Production
SustainableChemistry
BioChemicals
Technology Hype
GreenTechnologies GmbHphytomining
Screening with non endogenous, non biogenous substrates!
GreenTechnologies GmbH
Isoprene
Hemiterpenes (1) – isopentenol, isoamyl alcoholMonoterpenes (2) – essential oils, mentholSesquiterpenes (3) – essential oils, artemisininDiterpenes (4 ) – phytol, vitamin A, crocetinSesterterpenes (5) – waxesTriterpenes (6 ) – celastrol, squalene, steroidsTetraterpenes (7 ) – carotenes, xantophyllsPolyterpenes (X ) – rubber, gutta
Enzymes
AA, purines /pyrimidines
Enzymes
Pyrrole/pyrrolidine A. – nicotine, cocainePyridine/piperidine A. – coniineTropane Alkaloids – atropineQuinoline Alkaloids – cinchonaIndole Alkaloids – rauwolfiaPurine Alkaloids – caffeineSteroidal Alkaloids – kurchiIsoquinoline A. – opium
Tannins – epigallocatechinLignin – p-hydroxyphenyl L, syringyl LFlavonoids – quercetin, kaempferol
(Poly)phenolics
Enzymes
Unique Plant Chemicals
GreenTechnologies GmbHProduction
Diosgenin(Steroidsaponin)
Sitosterin (Triterpen)
Stigmasterin(Triterpen)
CortisonProgesteron
Fusarium,Curvularia
phytochemistry & phytominingplant metabolites & enzymes forexiting products
GreenTechnologies GmbHExpression & analysis
GreenTechnologies GmbHSynergy
GreenTechnologies GmbHTerpene Biosynthesis
Bouvier et al., 2005
GreenTechnologies GmbH
OPP
O O O
O OH OAc
O OH
Geranyldiphosphate
(-)-Limonene (-)-trans-Iso-piperitenol
(-)-Isopiperit-enone
(+)-cis-iso-pulegone
(+)-Pulegone (-)-Menthone (-)-Menthol
(+)-Mentofuran(+)-Neo-
menthol(-)-Menthyl-
acetat
OH
A
B
Storage
GreenTechnologies GmbH
Lim
onen
Pule
gon
L-M
enth
on
L-M
enth
ol
Men
thyla
ceta
t
Car
von
Neo
men
thol
Men
thof
uran
0,0
0,1
0,2
0,3
0,4
An
teil
Minze 18.23
1. Screening 2. Fusion 4. Evaluation3. Selection Application
phytodiversity
GreenTechnologies GmbH
Regeneration ofHybrid Plants
Cell division, Callus andShoot Regeneration
Parameter Donor Material andProtoplast Isolation
Parameter Cell Fusion
Analysis
Somatic Hybridization
GreenTechnologies GmbHImproving Biodiversity
0
500000
1000000
1500000
2000000
2500000
3000000
3500000
Lim
onen x1 x2
Pul
egon
Lim
onen
Men
thon
Men
thof
uran
Men
thyl
acet
atN
eom
enth
olM
enth
olP
uleg
onC
arvo
n
Lim
onen
Men
thon
Men
thof
uran
Neo
men
thol
Men
thol
Pul
egon
Car
von
Lim
onen
Men
thon
Men
thof
uran
Neo
men
thol
Men
thol
Pul
egon
Car
von
Lim
onen
Men
thon
Men
thyl
acet
atN
eom
enth
olM
enth
olP
uleg
onC
arvo
n
Lim
onen
Men
thon
Men
thof
uran
Men
thyl
acet
atN
eom
enth
olM
enth
olP
uleg
onC
arvo
n
Men
thon
Neo
men
thol
Men
thol
Pul
egon
3 Fusion 2.1 Fusion 2.2 Fusion 2.4 Fusion 2.6 Fusion 2.7 32
Are
a[c
ou
nts
]
GreenTechnologies GmbHMenthone
M. piperita
Biochemical complexity
plants build up
bacteria break down
Opportunities
GreenTechnologies GmbH
IndustryAltana AGBayer MaterialScience AGBayer Technology Services GmbHCognis Deutschland GmbH & Co. KGEvonik Degussa GmbHHenkel KGaALANXESS AG
AcademiaForschungszentrum Jülich GmbHFraunhofer IMEFraunhofer UMSICHTFraunhofer WKIISAS – Institute for Analytical SciencesUniversität BielefeldUniversität DortmundHeinrich Heine-Universität DüsseldorfUniversität Münster
Investors & FoundersProf. Dr. Detlev RiesnerSirius VentureDr. Martin Wilhelm, focus ehfNRW.BANK
InfrastructureBioanalytik Münster e.V.Bioindustry e.V.Bio-OWL e.V.PROvendis GmbHVCI NRW
CLIB2021: Industry, SME, AcademiaInvestors & Infrastructure
SMEArtes Biotechnology GmbHAqura GmbHBayGenetics GmbHbitop AGBioreact GmbHBIOTECmarin GmbHButalco GmbHCilian AGCognis Oleochemicalsc-LEcta GmbHDASGIP AGDirevo Biotech AGDohms Ingredients GmbHevocatal GmbHHöFer bioreact GmbHInsilico Biotechnology AGKaye Scholerm2p-labs GmbHMöller Group GmbH & Co KGNanotecMARIN GmbHNova Institut GmbHPATEV GmbH & Co. KGPharmedArtis GmbHPhytowelt GreenTechnologies GmbHProtagen AGRhein Biotech GmbHTaros Chemicals GmbH & Co. KGWEE-Solve GmbH
GreenTechnologies GmbH
AcademiaForschungszentrum Jülich GmbHFraunhofer IMEFraunhofer UMSICHTFraunhofer WKIISAS – Institute for Analytical SciencesUniversität BielefeldUniversität DortmundHeinrich Heine-Universität DüsseldorfUniversität Münster
Investors & FoundersProf. Dr. Detlev RiesnerSirius VentureDr. Martin Wilhelm, focus ehfNRW.BANK
InfrastructureBioanalytik Münster e.V.Bioindustry e.V.Bio-OWL e.V.PROvendis GmbHVCI NRW
SMEArtes Biotechnology GmbHAqura GmbHBayGenetics GmbHbitop AGBioreact GmbHBIOTECmarin GmbHButalco GmbHCilian AGCognis Oleochemicalsc-LEcta GmbHDASGIP AGDirevo Biotech AGDohms Ingredients GmbHevocatal GmbHHöFer bioreact GmbHInsilico Biotechnology AGKaye Scholerm2p-labs GmbHMöller Group GmbH & Co KGNanotecMARIN GmbHNova Institut GmbHPATEV GmbH & Co. KGPharmedArtis GmbHPhytowelt GreenTechnologies GmbHProtagen AGRhein Biotech GmbHTaros Chemicals GmbH & Co. KGWEE-Solve GmbH
GreenTechnologies GmbH
CLIB2021: Industry, SME, AcademiaInvestors & Infrastructure
IndustryAltana AGBayer MaterialScience AGBayer Technology Services GmbHCognis Deutschland GmbH & Co. KGEvonik Degussa GmbHHenkel KGaALANXESS AG
Sugar
Starch
Glucosesyrup
Sugar caneSugar beet
CornSeed
Value
R&D
Biomass
Amylase
Feedstock
Microbialintermediates
Pulpingenzymes
Plant, animal,microbial biomass
CLIB2021: Partnering with Ag-Biotechall over the Value-Chain
Enantiomericallypure drug
Polymers
PolylaminesSuccinic acid
Amines
Amino Acid
AdhesivesAdhesivePeptides
Enzyme
Ethanol
Superabsorber
Plastics
Consumer product
Intermediate
ButadieneAcetaldehyde
Acrolein
Chemical productsPyridines
Feedstock-Supplier
Agro-Industry
Chemical Industry
Consumer Industries
Shortens Time to Market
GreenTechnologies GmbH
Thank You for Your Attention!
Further Information:
www.phytowelt.comwww.clib2021.com
p.welters@phytowelt.com
Recommended