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José Bento Pereira Lima
Innovative Vector Control approaches:
Wolbachia
Transgenic mosquitoes
Pesticides, new molecules, Bacilos
First Regional Dengue Symposium
With 1.4 million cases throughout Brazil, dengue hits record
According to the latest epidemiological bulletin of dengue Ministry
of Health, were 1,463,776 probable cases of the disease recorded
from January 4 to 26 September in Brazil.
The previous record of 2013 was 1,452,489 people infected.
shutterstock.com/
vacine specific drugs Mosquito control
Products
7-10 days
eggs larvae and pupae adultos
Genilton J. Vieira / IOC Genilton J. Vieira / IOC
Life cycle
With visiting house to house in two months cyclesand in some areas the pending reaches 40%It is very difficult the vector control
Dengue mosquito - breeding
• Intracellular
bacteria
• Present in 20-
65% of all species
of insects,
including
mosquitoes that
bite humans.
WOLBACHIA
cytoplasmic incompatibility
X
X
X
X
0 20 40 60 80 100
Hatch rate %Hatching rate%
2011: Release of wMel in Yorkeys Knob and Gordonvale
Rio de JaneiroBrasil
GuangdongChina
Tri Nguyen Vietnam
CairnsAustralia
YogyakartaIndonésia
Gerenciamento do Projeto
National projects in progress
URCA TUBIACANGA
VALQUEIRE JURUJUBA
•Goal – evaluate RIDL technology (OX513A) in open field release
•Collaboration between Universidade de São Paulo and Moscamed Brasil
•Oxitec agreement – No bias in the evaluation
A field release validation for transgenic
Aedes aegypti population suppression
Program OverviewProduction -> Release -> Monitoring
Adult traps
Eggs produced
(4-6 million/week)
Rear release
generation
Separate Male
Pupae
Placed in release
device
Eggs -> Pupae
1 million/week
♂
Release males
Adults x 3 week
(0.5 to 1.5 million/week)
Monitoring
Hatch eggsEggs traps
Larvae screened
for presence of
Fluorescent
genetic marker
Mating
Itaberaba1400 houses
550 km²
Mandacaru600 houses
360 km²
~ 15 Km distance
Site Selection
PLOS Neglected Tropical Diseases | DOI:10.1371/journal.pntd.0003864 July 2, 2015
New Project – Jacobina - Bahia
80% 40%
Jacobina Suppression
Diretor da Vigilância Epidemiológica - Jacobina – José Alves Silva Junior“em 2012 Catuaba o IIP = 4,4%, Pedra Branca = 16,7%.......em 2014 Catuaba = 2,2% e Pedra Branca 2,2%, concluindo que nesse momento o importante é a prevenção”.
Aedes aegypti Production (UPAT)
Males for releases 1,5 million/week
● Actellic 300 CS was evaluated in Ivory Coast (Swiss TPH), Benin (LSHTM / CREC) and Tanzania(LSHTM / KCMC) study in cabin areas;
● Studies show a higher residual control than 6 months on surfaces such as cement and clay;
● organophosphate, pirimiphos-methyl, ideal for resistance management;
● New long-term formulation (microencapsulated suspension / CS).
New Products - adulticide
Mosquitoes come in contact withthe capsule on the treatedsurfaces: Capsule in mosquitolegs.
Capsules Actellic 300 CSAre intact after application: Capsules in cement
New Products - adulticide
New Products - adulticide
Bti PRESENTATIONS
mini-tabletes DT(tablete de uso direto)
granulado WG(grânulo dispersível em água)
0 cm 1 2 3 4 5 6
C1 C2 T A10
01 mini-tablete 6 g
50 L 100 L 300 L 500 L 10.000 L
New Products - larvicide
eficacy + than 60 days; choque effect in a few hours
sustentability no history of vector resistance!
safety potable water; no sensitizing/irritating; min. exposition
practicality doses and formules read to use; need no equipments
aplicability concentrated product; tolerates temperature variations
TECHNICAL REQUIREMENTS
16 C1 C2 T A
New Products - larvicide
New Products - larvicide
New Products - larvicide
No odorUsed for drinking waterProduct is under WHOPES
SumiLarv® 2MR is a mosquito larvicidaldevice and its active ingredient, pyriproxyfen is incorporated into polymer resin as anewest formulation “MR”
Spinosad
Dissemination of Pyriproxyfen
DENGUE - Amazônia
Contamination byinsecticide impregnated
Contamination of theaquatic environment
DENGUE - Amazônia
Results
larva muerta pupa muerta eclosion inhibicion
8%0%
92%
8%
18%
61%
21%
89%
1% 3%
96%
4%
% Mortality of larvae and pupae hatching inhibition of mosquitoes Before, During and After the Intervención with pyriproxifen
ANTES DURANTE
DESPUES
DENGUE - Amazônia
• Mosquitoes spread the pyriproxifen to ≈ 0.5 km (500 meters).
• 100% of the houses were infested with pyriproxifen and 94% of sentinel breeding.
• The pyriproxifen causes mortality of larvae and pupae.
• The mortality of larvae and pupae in sentinel breeding is higher as is closer to the pyriproxifen broadcasting stations.
DENGUE - Amazônia
MANACAPURU
DENGUE - Amazônia
DENGUE - Amazônia
Meses Aedes
aegyptiAedes
albopictus Limatus Culex Spp
Nov. 2013 1128 3155 185 37
Dez.2013 1556 5881 87 30
Jan.2014 1533 3154 119 7
Feb. 2014 82 741 130 9
Mar.2014 288 930 89 6
Before Abr.2014 224 695 183 4
Mai.2014 286 965 371 0
Jun.2014 341 677 76 0
Jul.2014 155 772 31 0
Ago.2014 87 587 2 0
Set.2014 109 1018 0 6
Out.2014 530 779 10 2
Dez.2014 543 1062 91 22
Jan.2015 363 1272 149 2
Feb.2015 238 542 131 0
Total 7463 22230 1654 125
Meses Aedes
aegyptiAedes
albopictus Limatus Culex Spp
Mar.2015 130 371 0 0
DURANTE Abr.2015 121 306 0 7
Mai.2015 65 130 0 0
Jun.2015 11 56 0 0
Jul.2015
Total 327 863 0 7
DENGUE - Amazônia
Monitoring and control in Itacoatiara - AM
0
10
20
30
40
50
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25
Positivity and average weekly eggs
Positivity Média+
New RNAi methods for vector control
dsRNA targeting
β-tubulin
Temephos
Increased toxicity to Temephos
Insect Molecular Biology (2013) 22(6), 648-658
dsRNA targeting
P-glycoprotein
Increased larvae mortality
Journal of Insect Science: 2013;13:69
dsRNA-treated larvae Phenotype in larvae
Parasites & Vectors 2015, 8:96
dsRNA targeting both testis genes and female sex
determinationReduced fertility
in males
Inhibition of female development
dsRNA-treated larvae Phenotype in adult mosquito
Rafael Freitas IOC - Fiocruz
Margareth Capurro – USP
Sergio Luz – Fiocruz Amazonas
Emerson Bernardes – Forest
Washington Ferreira – Syngenta
Haroldo Gomes -
Acknowledgment
OBRIGADO
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