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From Lunch to your Liver. Magnified look of salad’s journey from the bowl to the belly. Salad. Confocal 40X. DAPI. Overlay Confocal and DAPI. DIC and Fluorescence micrograph superimposed. Onion Nucleus Oil Immersion Fluorescence (DAPI) 100X. Onion nucleus Oil immersion DIC 100X. - PowerPoint PPT Presentation
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From Lunch to your LiverFrom Lunch to your Liver
Magnified look of salad’s journey Magnified look of salad’s journey from the bowl to the belly.from the bowl to the belly.
Salad
Confocal 40X DAPI
Overlay Confocal and DAPI
DIC and Fluorescence micrograph superimposed
Onion nucleusOil immersionDIC 100X
Onion NucleusOil ImmersionFluorescence (DAPI)
100X
Area -98518Perimeter -1225Pixel Density -1.620 pixels/micron
Tomato Convolutions
DIC 40X Sharpening Kernal
Smoothing Kernal
Convolution -1 -1 -1 -1 24 -1 -1 -1 -1
Fluorescent Cheek Cells (DAPI)
10X
Cheek Cells
40X Bright Field 40X Hoffman
100X Phase (oil immersion) 60X Confocal
Un-erupted Pig tooth
4X Bright field 10X Bright field
40X Bright Field 40X Phase contrast
Pig Liver
4x Bright field 10X Bright field 40X Bright Field
40X Phase
Adjust BrightnessAndContrast
I used the image calculator and subtracted a picture of the background. Then I adjusted the brightness and contrast.
Muscle tissue10X Bright field
Muscle tissue 10X Dark Field
Adjusted Brightness and contrast
LUT manipulation for 10x Muscle slide
Changed to Grey ScalePixels 127-255127 ….. 255R G B R G B0 0 255 0 255 255
Adipose Tissue
40X Bright field
40X Phase
RGB SplitMathAdjust Brightnessand Contrast
Adjust Brightnessand Contrast
Enhance me
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