Fusion cell gene profiling Identify mbo and CG15252 enhancer Molecular mechanism of target gene...

•Fusion cell gene profiling

•Identify mbo and CG15252 enhancer

•Molecular mechanism of target gene regulation by Dys::Tgo

•Dys::Tgo activation of CG13196

•Dys:Tgo activation of CG15252

•Identify dys fusion cell enhancer

dys-gal4::UAS-CD8-GFP btl-gal4::UAS-CD8-GFP

dys-gal4::UAS-CD8-GFP dys-gal4::UAS-CD8-GFP ; dys/dys

•Identify dys target gene

dys genomic structure

FTF Q72L FTF T31N

F2F elution

LP05454

Exon1 2 3 4 5 6 7 8 9 10

1.7kb2.7kb

3.8kb

3.8kb

3.8kb

CG13196 enhancer

Expression patterns of CG13196 enhancer and TCGTG enhancer

Early S15

4xTCGTG GFP Dys

S16

CG13196 GFP Dys

CG13196 4xTCGTG GFP

S15

S16

Dys Dys

Ectopic expression of dys can activate CG13196 and TCGTG enhancer ectopically

CG15252 5’ upstream

Exon1

3.2kb

9.0 kB 5’ upstream

0.8 kb TCGTG cluster

TCGTG TCGTG TCGTG

Spalt is not required for CG15252 activation by dys in DT

Need to do:CG15252 expression in Spalt mutants

CG15252 Dys CG15252 Dys

btl-gal4;UAS-dys btl-gal4;UAS-spalt;UAS-dys

Ectopic dys can activate mbo-lacZ ectopically

mbo-lacZ-Dys

btl-gal4, UAS-dys

mbo 3’

TCGTG

407bp

ACGTG

Conclusion

•There are other transcriptional activators turns on at late stages or changes in chromatin structure.

• Spalt is not required for CG15252 expression

•There is no transcriptional inhibition at early stage

• dys target genes can only be turn on at late stages (late 14 or early 15)