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Genetic Genealogy, History and Prehistory, and DNA Ancestry Tracing. “DNA and Your Health” Seminar Presented by Donald N. Yates, Ph.D. A funny thing happened to me on the way to the synagogue. Rabbi Yitzak Levy. Rabbi Michael Lerner. Family Tree DNA, Houston University of Arizona. - PowerPoint PPT Presentation
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Genetic Genealogy, History and Prehistory, and DNA Ancestry Tracing
“DNA and Your Health” Seminar
Presented by
Donald N. Yates, Ph.D.
A funny thing happened to me on the way to the synagogue
Rabbi Yitzak LevyRabbi Yitzak Levy Rabbi Michael LernerRabbi Michael Lerner
Skorecki K, Selig S, Blazer S, Bradman R, Bradman N, Waburton PJ, Ismajlowicz M, Hammer MF (Jan. 1997).“Y chromosomes of Jewish priests.” Nature 2;385(6611):32.
Brief History of DNA Testing
• Gregor Mendel (19th cent.)• George Darwin and cousin
marriage• James Watson & Francis
Crick (double helix, 1953)• Biotechnology (1970s-80s)• Cavalli-Sforza • Human Genome Project• Cohen gene (1997)
Review 1
• George Darwin
• Double Helix DNA
• Where it all started
• Cohen Modal Haplotype
• Oxford Ancestors
• Family Tree DNA
Why Do a DNA Test?
• Determine if two people are related • Determine if two people descend from the same
ancestor • Find out if you are related to others with the same
surname • Prove or disprove your family tree research • Go beyond brick wall in genealogy• Provide clues about your ethnic origin • To find out about genetic risk factors in health
Short Tandem Repeats (STRs)
the repeat region is variable between samples while the flanking regions where PCR primers bind are constant
7 repeats
8 repeats
AATG
Homozygote = both alleles are the same length
Heterozygote = alleles differ and can be resolved from one another
ABI Prism 310 Genetic Analyzer
capillary
Syringe with polymer solution
Autosampler tray
Outlet buffer
Injection electrode
Inlet buffer
M8 + F8 M9 + F9 MA + FA MB + FB MC + FC MD + FD ME + FE MF + FF I I I I I I I I M4 …. + …. F4 M5 …. + …. F5 M6 …. + …. F6 M7 …. + …. F7 I I I I M2 …. …. …. + …. …. …. F2 M3 …. …. …. + …. …. …. F3 GrandFather I I GrandMother I I M1 …. …. …. …. …. …. …. + …. …. …. …. …. …. …. F1 Father I Mother I I I I Brother M0 F0 Sister
Inheritance ChartInheritance Chart
Branches and Twigs
Haplogroups are the descents or mega-families that characterized early human
migrations. They are normally associated with geographical regions. Examples: R1b
(Western Atlantic European), I (northern Europe), J (Jewish, Middle Eastern).
Haplotype One person's set of values for the markers that have been tested. Two individuals that
match on all markers but one, have two distinct haplotypes. (One-step mutation)
Atlantic Modal Haplotype
DYS388 12DYS390 24DYS391 11DYS392 13DYS393 13
DYS394 14 (also known as DYS19)
If you have one mutation in either direction, then you are AMH 1.15+. The AMH 1.15
haplotype is also referred to as the Atlantic Modal Cluster or AMC. Generally 1.15+ puts
you in haplogroup 1 (H1), but not always.
Y Chromosome
• Males: XY• Females: XX• Father to Son• Follows Surname• Y STRs: Short Tandem Repeats• Mutation rate fast• YHRD, Ysearch• Non-paternity events• 12 . . . 24 . . . 37 . . . 63 . . . 98• Surname Projects
Review 3
• D loop
• HVR I and HVR 2
• Cambridge Concordance
• Vincent Macaulay
• Phylogeny
• Haplogroup, haplotype
Uses of Autosomal Tests
• Paternity– Gene Tree & Terry Carmichael
• Relationship testing (siblings adopted)• Police work – CODIS profiles, OmniPop• DNA Fingerprint Test• DNAPrint – biogeographical markers• Inherited disease
Examples of Autosomal Tests
• DNA Fingerprint Test• Eurasian1.0• EURO DNA 2.0• SNP testing• Genetic screening• Genome-wide genetic research
Review 4
• Autosomal• Recombination• Allele• Ancestry Informative Marker• DNAPrint• DNA Fingerprint Test• Base pairs• STRs
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