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Calcium Chloride / Gentamicin / Glucose / Magnesium Sulfate /Potassium Chloride / Potassium Phosphate / Sodium Bicarbonate /Sodium Chloride / Sodium Lactate / Sodium Pyruvate
m-HTF : HEPES
HTF medium /m-HTF medium
HTF medium○
○ No animal derivative is contained.
Kitazato HTF medium is suitable to use in multi-gas incubator.For washing sperm, insemination and culture of earlyembryos.
○ m-HTF mediumKitazato m-HTF medium is suitable to use in ambient atmosphere. For handling and manipulation of oocytes/embryos.
HTF medium / m-HTF medium
COMPONENTS
with Human Serum AlbuminHTFS-100
HTFMS-100100mL
Code Details
93441
93443
Order Number Contents
Sterile Filtration / Sterility Test / pH 7.2-7.6 / Osmolality 270-295mOsm/kg / Endotoxin <0.25EU/mL /Mouse Embryo Assay ≥80% / Mouse Oocyte Survival Test ≥80%
Storage: 2-8℃ / Shelf life: HTF= 90 days, m-HTF=180days
QUALITY CONTROL
Cell Culture
Enlargement
CONTACT : Kitazato Corporation International Sales Division1-1-8 Shibadaimon, Minato-ku, Tokyo 105-0012 JAPAN
+81-3-3434-1653
+81-3-3434-1652
Copyright© Kitazato Corporation All Rights Reserved. | March 11, 2019 Ver. 1
www.kitazato.co.jpURL Mail trading@kitazato.co.jp TEL
FAXSpecification may change without pre-noticefor purpose of product improvement.
Kitazato Logo is trademarked.HEAD OFFICE : 81 Nakajima, Fuji, Shizuoka 416-0907 Japan
TEST RESULTSFo
rmat
ion
rate
(%)
KITAZATO COMPANY A KITAZATO COMPANY AHTF m-HTF
100
50
Form
atio
n ra
te (%
)
50
94.8%
0
91.2%91.6% 90.5%
293320293320
287317287317
147155
142155
147155
100
0
NS NS
142155
Stable pH value in medium is a very important factor for oocytes and embryos. Kitazato Corporation Co., Ltd has improved the composition of HTF medium. In the result, the pH value rise was minimized when the medium was exposed in ambient atmosphere. On the other hand, the value quickly returned to pH7.4 when the medium was trans-ferred back into a multi-gas incubator.
●Maintain stable pH value
Blastocyst formation rate was 94.8%, when 2-Cell mouse embryos (ICR) were cultured in KITAZATO HTF medium for 72 hours. Slightly better result was obtained with KITAZATO HTF medium compared with another brand.
●Mouse embryo assay
HTF
Blastocyst formation rate was 91.2%, when 2-Cell mouse embryos were processed in the m-HTF medium for 30 minutes and then cultured with KSOM medium for 72 hours. Slightly better result was obtained with KITAZATO HTF medium compared to another brand.
m-HTF
(MEA)
Comparison of pH rise underthe ambient atmosphere. (30min.)
pH
time
KITAZATO
(min.)time(min.)
8.00
7.90
7.80
7.70
7.60
7.50
7.40
7.305 10 15 20 25 30
Comparison of pH changeover the time in gas incubation
pH
8.00
7.90
7.80
7.70
7.60
7.50
7.40
7.3030 60 90 120 150 180 210 240 720
KITAZATO-HTFCOMPANY_A-HTFCOMPANY_B-HTF
KITAZATO-HTFCOMPANY_A-HTFCOMPANY_B-HTF
KITAZATO
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