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Healthcare workers' acceptance and performance of point-of-care CD4 testing in Dar es Salaam. Nichole Arnett, Karen Chang, Mary Schmitz, Ruth Lemwayi, Patrich Rwehumbiza, Michael Mwasekaga, Luciana Kohatsu, Omotayo Bolu, Fasta Mosha, Sehin Birhanu, Lydia Lu, John Nkengasong - PowerPoint PPT Presentation
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International Lab BranchCenter for Global Health / Division of Global HIV/AIDS
Nichole Arnett, Karen Chang, Mary Schmitz, Ruth Lemwayi, Patrich Rwehumbiza, Michael Mwasekaga, Luciana Kohatsu, Omotayo Bolu,
Fasta Mosha, Sehin Birhanu, Lydia Lu, John Nkengasong
Larry Westerman, PhD MMScClinical and OI Monitoring Team International Laboratory Branch
Division of Global HIV/AIDSCDC , Atlanta, Georgia
Healthcare workers' acceptance and performance of point-of-care CD4
testing in Dar es Salaam
Specimen Collection For Pima CD4 Testing
Fingerstick-Direct
Fingerstick-Microtube
Pima-Venous
Preference
Fingerstick-Direct
Fingerstick-Microtube
Pima-Venous
EaseOf
Use
Failed CD4
Result
Invalid
TestAccuracy
2nd
3rd
1st
1.8
3.0
1.7
1=very easy5=very difficult
9.9%
4.9%
0.9%
10.1%
8.6%
7.7%
R2= 0.82Bias -20
R2= 0.88Bias 0
R2= 0.89Bias -10
1. Collect supplies: - Lancet/alcohol/gauze2. Identify patient 3. Clean finger with
alcohol and dry
4. Select target site- - 3rd or 4th Finger, preferred - Off center from finger pad - lancet blade should cut vertical to fingerprint ridges
5. Hold finger firmly6. Place a new sterile lancet on
the target site7. Activate lancet while applying
firm downward pressure
7. With your finger, apply pressure on opposite side of lanced finger
8. Wipe away the first drop of blood with a sterile gauze
Fingerstick Technique is important for CD4 testing
Slighthly more variation and errors with fingerstick compared to venous
Collection of fingerstick specimen in an EDTA microtube more like venous blood
Recommended