View
220
Download
4
Category
Preview:
DESCRIPTION
實驗室產品、資訊和科學娛樂
Citation preview
AUTUMN2012
:
Applied Biosystems p10
TRIED & TRUE EXCITING & NEW
TOPO cloning kits p16
UltraPure p12
BIOPROBES 67JOURNAL FOR CELL BIOLOGY APPLICATIONS JUNE 2012
Going viralFluorescent probes to image viruses and their host cell interactions
Ready-to-use reagents shift the focus to results
Plus: ABfinity recombinant antibodies
Oregon Green 488labeled paclitaxel microtubules
( Peter Caie Mol Cancer Ther 9:19131926)
etoposide U2OS CellEvent Caspase-3/7 Green Detection Reagent () Hoechst 33342 () MitoTracker Deep Red FM () Alexa Fluor 546 Phalloidin ()
lifetechnologies.com/imaging
: :
BioProbes BioProbes
lifetechnologies.com/bioprobes
FLUORESCENCE IMAGING
Life TechnologiesTM 2
13 5 PCR
Cloning
14
19
30
28
Life in the lab
> Life in the lab Life Technologies
> lifetechnologies.com/selection
> Facebook Twitter lifetechnologies.com/socialhub
> APP ( Invitrogen Calculatorcell imaging CloningbenchCytometry )apps lifetechnologies.com/appsapps.
Life in the lab
Dr. Shinya Yamanaka
17 Xavier
23 Matt Might
13 InvitrogenTM 10 Applied Biosystems 18 Gibco 26 Molecular Probes 24 Novex 10 TaqMan 8 Ambion 4 IonTorrentTM
31
31
22
27 Molecular Probes
3 Life TechnologiesTM
1,000
18 1,000 Ion PGM Ion Personal Genome Machine (PGM) Q30 DNA RNA lifetechnologies.com/pgm
NEW productBigger screen, smaller sample 10 ng DNA
4 Ion AmpliSeq Comprehensive Cancer Panel 16,000 Ion AmpliSeq Inherited Disease Panel 700 300 3 10,000
lifetechnologies.com/ampliseq
Ion RNA-Seq
RNA-Seq cDNA RNA
Ion 318 small RNA
small RNA 500,000 miRBASE
microarray
Ion RNA-Seq ~200 RefSeq
* Ion 318
lifetechnologies.com/ionrnaseq
* Ion Torrent lifetechnologies.com/ioncommunity Gene expression proling using ion semiconductor sequencingapplication note.
Be PARTRevoluTIoN of the
Ion TorrentTM 4
2000
13 30
1,000
36 Ion Proton 1,000Baylor 36
lifetechnologies.com/baylorproton
Proton
Dr. Richard Gibbs Baylor
Ion Reporter Ion PGM Ion Proton Ion Reporter DNA
lifetechnologies.com/ionreporter
bLazIng The paTh To The $1,000 genoMe
Ion TorrentTM5
Its easy to get what your research lab needs at
lifetechnologies.com
FIND DECIDE BUY DISCOVER
Life TechnologiesTM 6
27 a.m.
( 27 a.m.)
LB agar 65C
elution
() gel
gel
Kimwipes
Paralm M gel loading
( oligos)
FRoM The benCh
7 Life TechnologiesTM
rNAi:
RNAi
1990
Richard Jorgensen
()
( AIDS
)
RNAi
Ambion RNAi
Silencer Select siRNA mirVana
miRNA
Silencer Select siRNA
Silencer Select siRNA
Dr. Gavin Robertson
SILENCER SeLeCT SIRna
Silencer Select siRNA Gavin Robertson
lifetechnologies.com/sirnatestimonial
mirVana mimics and inhibitors Alexander (Sasha) Vlassov mirVana miRNA
lifetechnologies.com/inventorsirna
Rnai RNAi
lifetechnologies.com/rnaiwebinar
Silencer Select siRNA Susan Magdaleno Silencer Select siRNA
lifetechnologies.com/inventorsirna
ncbi.nlm.nih.gov/pubmed/12354959
aMbIon: Rnai
ambion 8
RNA PCR Ambion Cells-to-CT Kits 7 PCR
lifetechnologies.com/cellstoct
Rna aMbIon
RNA
Ambion Ambion
lifetechnologies.com/publishrna
SAvE 35% on Ambion cells-to-ct kits
P910019 lifetechnologies.com/promotions
Cells-to-CT kit lifetechnologies.com/cellstoct
cells-to-ct
cells-to-ct Kits
pcr
ambion9
TaQMan aSSaYS QuanTSTuDIo 12K FLeX
Biodonostia David Otaegi TaqMan MicroRNA Assays QuantStudio 12K Flex
microRNAs
lifetechnologies/taqman
pcr
PCR
think new! thINK bIGGEr than ever savings!
10applied biosystems
pCR pCR
PCR (PCR)
QuantStudio 12K Flex PCR PCR
lifetechnologies.com/dpcrdebate
SYbr SElEct MAStEr MIX
DNA
SYBR GreenER
FrEE SAMplE !
!
UDG
PCR
lifetechnologies.com/sybr
SYbR SeLeCT GreenER
11 applied biosystems
UltraPure UltraPure
lifetechnologies.com/promotions
P873968
Fast PCR Master Mix* 5 * GeneAmp Fast PCR Master Mix ( 4362070250 rxns) AmpliTaq Gold Fast PCR Master Mix ( 4390939250 rxns)
P910070
lifetechnologies.com/promotions
PCR
Veriti PCR 2720 PCR
PCR ()
buY 1 GEt 1 FrEE
PCR
(MDTV (Multiplex Dynamic Temperature Verication) )
305
MDTVlifetechnologies.com/mdtv
SAvE 50%
Mdtv pcr
12applied biosystems
dNTP PCRInvitrogen dNTP lifetechnologies.com/promotions
P910083
Invitrogen dNtp
Specicity Fidelity
PCR
() PCR
DNA
dNTP PCR
dNTPPCR
dNTP
PCR agarose gel PCR
DNA Taq
MgCl2
annealing45C55CPCR
Tm
Tm
Taq PCR primer-dimers
Taq
annealing
2
3
4
5
1
5 PCR
13 Invitrogen
Pico precision. Mega flexibility.
Dr. Jon Chesnut GeneArt Precision TALs
lifetechnologies.com/meettheinventor
GeneArt Precision TALs
GeneArt Precision TALs DNA DNA targeting targets
GeneArt Precision TALs
lifetechnologies.com/tal
Vector NTI Express PC Mac
cloning
lifetechnologies.com/vectornti
FrEE trIAl veCToR nTI EXPRESS SoFTWaRe
!
cloning genome targeting engineering
14Invitrogen
! ! !
GeneArt Oligo Primer
lifetechnologies.com/geneart
GeneArt Seamless Cloning and Assembly Enzyme Mix
DNA PCR PCR DNA (A14606)
GeneArt Seamless PLUS Cloning and Assembly Kit
DNA 30 DNA (A14603)
GeneArt Site-Directed Mutagenesis PLUS System
DNA (A14604)
GAtEWAYGateway Gateway 1,500
lifetechnologies.com/gateway
The secrets in the mix Federico Katzen GeneArt seamless cloning assembly
lifetechnologies.com/meettheinventor
Flexible expression. Any vector.Gateway
lifetechnologies.com/meettheinventor
dNA
15 Invitrogen
SAvE 50% on topo cloning kits
Invitrogen TA TOPO GeneArt
> (ligation)
> TA cloning PCR
> TOPO cloning
> GeneArt Seamless Cloning Assem-bly
lifetechnologies.com/cloningchoices
Jon Chestnut
TOPO cloning lifetechnologies.com/meettheinventor
TOPO PCR cloning TOPO cloning 5 PCR 95%
TOPO cloning kits cloning PCR Gateway
lifetechnologies.com/choosetopo TOPO
TOPO cloning kits (compentent cells) 5 ! P910094
lifetechnologies.com/elevatepcr
eLevate Your ReSeARCH
16Invitrogen
55
Day
0D
ay 1
Day
24
Seed cells so they are 7090% confluent at the time of transfection.
Lipofectamine LTX Reagent
Cells
DNA and PLUS Reagent
Visualize/analyze transfected cells.
Add DNA-reagent complex to cells.
Incubate.
Add diluted DNA to each tube of diluted Lipofectamine LTX Reagent (1:1 ratio).
Dilute DNA in Opti-MEM Medium, then add Plus Reagent.
Dilute 4 amounts of Lipofectamine LTX Reagent in Opti-MEM Medium.
55
Day
0D
ay 1
Day
24
Seed cells so they are 7090% confluent at the time of transfection.
Lipofectamine LTX Reagent
Cells
DNA and PLUS Reagent
Visualize/analyze transfected cells.
Add DNA-reagent complex to cells.
Incubate.
Add diluted DNA to each tube of diluted Lipofectamine LTX Reagent (1:1 ratio).
Dilute DNA in Opti-MEM Medium, then add Plus Reagent.
Dilute 4 amounts of Lipofectamine LTX Reagent in Opti-MEM Medium.
55
Day
0D
ay 1
Day
24
Seed cells so they are 7090% confluent at the time of transfection.
Lipofectamine LTX Reagent
Cells
DNA and PLUS Reagent
Visualize/analyze transfected cells.
Add DNA-reagent complex to cells.
Incubate.
Add diluted DNA to each tube of diluted Lipofectamine LTX Reagent (1:1 ratio).
Dilute DNA in Opti-MEM Medium, then add Plus Reagent.
Dilute 4 amounts of Lipofectamine LTX Reagent in Opti-MEM Medium.
55
Day
0D
ay 1
Day
24
Seed cells so they are 7090% confluent at the time of transfection.
Lipofectamine LTX Reagent
Cells
DNA and PLUS Reagent
Visualize/analyze transfected cells.
Add DNA-reagent complex to cells.
Incubate.
Add diluted DNA to each tube of diluted Lipofectamine LTX Reagent (1:1 ratio).
Dilute DNA in Opti-MEM Medium, then add Plus Reagent.
Dilute 4 amounts of Lipofectamine LTX Reagent in Opti-MEM Medium.
55
Day
0D
ay 1
Day
24
Seed cells so they are 7090% confluent at the time of transfection.
Lipofectamine LTX Reagent
Cells
DNA and PLUS Reagent
Visualize/analyze transfected cells.
Add DNA-reagent complex to cells.
Incubate.
Add diluted DNA to each tube of diluted Lipofectamine LTX Reagent (1:1 ratio).
Dilute DNA in Opti-MEM Medium, then add Plus Reagent.
Dilute 4 amounts of Lipofectamine LTX Reagent in Opti-MEM Medium.
55
Day
0D
ay 1
Day
24
Seed cells so they are 7090% confluent at the time of transfection.
Lipofectamine LTX Reagent
Cells
DNA and PLUS Reagent
Visualize/analyze transfected cells.
Add DNA-reagent complex to cells.
Incubate.
Add diluted DNA to each tube of diluted Lipofectamine LTX Reagent (1:1 ratio).
Dilute DNA in Opti-MEM Medium, then add Plus Reagent.
Dilute 4 amounts of Lipofectamine LTX Reagent in Opti-MEM Medium.
55
Day
0D
ay 1
Day
24
Seed cells so they are 7090% confluent at the time of transfection.
Lipofectamine LTX Reagent
Cells
DNA and PLUS Reagent
Visualize/analyze transfected cells.
Add DNA-reagent complex to cells.
Incubate.
Add diluted DNA to each tube of diluted Lipofectamine LTX Reagent (1:1 ratio).
Dilute DNA in Opti-MEM Medium, then add Plus Reagent.
Dilute 4 amounts of Lipofectamine LTX Reagent in Opti-MEM Medium.
Lipofectamine 20 48,000
Dr. Xavier de Mollerat du Jeu
lifetechnologies.com/tfinsights
lipofectamine
Lipofectamine LTX RNAiMAX
FrEE SAMplE of lipofectamine ltX or rNAiMAX
Lipofectamine LTX PLUS Lipofectamine RNAiMAX siRNA/miRNA Neon
>90% 30
7090% DNA 6080% siRNA/miRNA 20%
lipofectamine
DNA siRNA/miRNA DNA PureLink Plasmid DNA Miniprep Kit 0.51 g/L DNA 1520% siRNA Ambion Silencer Select siRNA mirVana miRNA mimics and inhibi-tors
1 4
25
3
dNA sirNA
dAY 24dAY 1
dAY 0
7090%
4 Lipofectamine LTX Opti-MEM
DNA Opti-MEM PLUS
Lipofectamine LTX DNA (1:1)
DNA-
/
lipofectamine
lifetechnologies.com/lipofectamine Lipofectamine
Lipofectamine
Xavier
17 Invitrogen
Countess Countess Countess
FrEE countess care Kit
Countess 30
lifetechnologies.com/countess Countess
Gibco
!100 Gibco (10 x 50 mL) +
lifetechnologies.com/promotions
P910803
gibco 18
10 8 - iPS (John Gurdon) 2012 Life Technologies
Congratulations!
(PAF) : PAF
(GladstoneInstitute of Cardiovascular Disease)1993
(iPSC)
iPSC
50
iPSC
Dr. Shinya Yamanaka iPS
Q
DR. ShInYa YaManaKaGibco
gibco19
SAvE 20%KnockOut (SR) 2,400
KnockOut SR (1082802810828010) 8
P910111
KnockOut SR lifetechnologies.com/knockout
Essential 8 PSC (A14666SA)
KnockOut SR XenoFree CTS Kit (A1099202)
Cellular Dynamics International Emile Nuwaysir Essential 8
MEF Essential 8
lifetechnologies.com/essential8interview
DynaMag-5 1116mm DynaMag-5 8 () lifetechnologies.com/magnets
(PSC)
lifetechnologies.com/culturepsc
gibco
20 50
gibco 20
iPSCChad Cowan CytoTune-iPS Sendai Reprogramming Kit
YouTube lifetechnologies.com/ipscvideoCytoTune lifetechnologies.com/cytotune
(iPSC) Life Technologies
(lentivirus) (nonintegrating) (Sendai virus episomal vectors)Invitrogen
lifetechnologies.com/reprogramming
CytoTune-iPS Sendai Reprogramming Kit (A1378001)
iPSC high-titer lentiviral iPSC reprogramming particles
lentiviral iPSC reprogramming particles lifetechnologies.com/lenti
Invitrogen episomal vectors iPSC (A14703)
lifetechnologies.com/reprogramming
21 InvitrogenTM
TECHTALKTIPS & TRICKS FROM THE LIFE LAB
T-REx expression system clonepcDNA6/TR clone lacZ
clone tetracycline -gal tetracycline -gal clone clone
T-REx Tet-reduced FBS
FreeStyle CHO-S FreeStyle CHO Expression Medium /
1:1,000 (1:200) Anti-Clumping Agent (0010057AE) Anti-Clumping Agent
Anti-Clumping Agent Anti-Clumping Agent
lifetechnologies.com/vectors
()
ph.dIvA Diva April Brite
lifetechnologies.com/phdiva Diva PCR
lIFe
Life TechnologiesTM 22
Matt Might Might
Might matt.might.net
PhD
PhD
The ILLuSTRaTeD guIDe To a phD
23 Life TechnologiesTM
Bolt Bis-Tris Plus Gels ! Western Blot wedge well
lifetechnologies.com/bolt
novex 24
FrEE StANdArdNativePAGE gelsNativeMark ladder
15 kDa 10 MDa pH (pI)
lifetechnologies.com/nativeP910119
...
lifetechnologies.com/multiplexelisa
FrEE poStEr
SAvE oN Abfinity ANtIbodIES ANd SEcoNdArY ANtIbodIES
pathwayMAPKAkt/mTORJAK/STAT
pathway
For research use only. Not intended for any animal or human therapeutic or diagnostic use. 2012 Life Technologies Corporation. All rights reserved. The trademarks mentioned herein are the property of Life Technologies Corporation or their respective owners. CO02269 0412
INFLAMMATION
ABnity
ABnity recombinant oligoclonal antibodies ABnity
ABnity lifetechnologies.com/abnity
P910879
lifetechnologies.com/bioprobes BioProbes
ELISA
MuLTIpLeX vS. SIngLepLeX
novex25
Alexa Fluor 790 secondary antibody conjugates Alexa Fluor 680Alexa Fluor 790 blot stripping
lifetechnologies.com/AF790dyes
Alexa Fluor 680 Alexa Fluor 790 7
P910751
Molecular Probes Illuminated Cell
SAvE 35% SAvE 30%
Molecular Probes Click-iT EdU Alexa Fluor
CellLight
lifetechnologies.com/mymolecularmoment
1
2
3
Alexa Fluor
100 g IgG 90
lifetechnologies.com/simplelabeling
monoclonal antibody labeling kit6.5
P910126
A TRENd TOwARd ExcEllEncE
Molecular probes 26
Molecular Probes ()Attune Attune Attune
lifetechnologies.com/attune
Molecular Probes
lifetechnologies.com/flowresources
4 CD marker
lifetechnologies.com/primaryantibodies
() 4 P910863
FrEE SlIdES
SAvE 60%
Tali Tali 3 ()
GFP & RFP
Tali 500 slide
P910786
lifetechnologies.com/tali
Molecular Probes
30 Molecular Probes
lifetechnologies.com/probeswebinars
Molecular probes27
()
1
1
protocol 30 30
4
3
3
RFID protocol ()
2
2
2012 LED Bluetooth qPCR XIII
1948 You havent come a long way, baby.
LAB OF THE FuturE
Life TechnologiesTM 28
George Armstrong
1889
The Gross Clinic (1875) The Agnew Clinic (1889) Both by Thomas Eakins.
DNARNA
8
10
( Pipettors)
Carrier
9
Pipette
10
6
7
8 9
5
64 75
Life TechnologiesTM29
Jesse Poland USDA Poland Leibniz Nils Stein Ion PGM (genotyping by sequencing, GBS)
GBS microarray GBS SNP GBS
GBS microarray
uSDA 2012 150
SNP 1,000
Life Technologies lifetechnologies.com/plants
Ion PGM
GBS SNP GBS Ion PGM GBS
protocol - DNA (RAD)
(SNP) SNP
SNP
DNA
Jesse GBS lifetechnologies.com/gbs
oN YouR Mark
Ion TorrentTM 30
2012 12 31
Life Technologies
(037)625-816 (Ambion Applied Biosystems ) ()
(04)2386-3198 (Ambion Applied Biosystems )() ()
(02)2790-2222PCR Ambion Applied Biosystems
()
micropipettes ()
Christina S.
Koushikul I.
200 6 CD Keith K.
parabalara L.
Kirsi D.
Sydney h.
Tatiana v.
Louise h.
Mimi C.
Justin Q.
Invitrogen Facebook
Manish B
Join the fun at facebook.com/lifetechnologies
31 Life TechnologiesTM
(supply center)
lifetechnologies.com
supplycenterrequest@lifetech.comsupply center
21238 | Phone 02-2358 2838 | Toll Free 0800-251 326207-1 | Phone 07-226 1886 | Toll Free 0800-016 157
www.lifetechnologies.com 2012 Life Technologies Corporation. All rights reserved. The trademarks mentioned herein are the property of Life Technologies Corporation or their respective owners. AmpliTaq and TaqMan are registered trademarks of Roche Molecular Systems, Inc. Bluetooth is a registered trademark of Bluetooth SIG, Inc. CytoTune is a registered trademark of DNAVEC, Inc. Facebook is a registered trademark of Facebook, Inc. Kimwipes is a registered trademark of Kimberly-Clark Worldwide, Inc. Mac is a registered trademark of Apple, Inc. Parafilm is a registered trademark of American Can Co. Twitter is a registered trademark of Twitter, Inc. YouTube is a registered trademark of Google, Inc.
Recommended