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Kolida S | Hernandez O | OHara SP| Rastall RA|
Contact: Dr Sofia Kolida | R&D Director | OptiBiotix Health PLC |Tel: ++ 44 7585001450|
BackgroundLactobacillus plantarum LPLDL
Ò is a probiotic with establishedcholesterol reducing activity in normal to mildly hypercholesterolemicadults. Probiotics can reduce cholesterol by various mechanisms:
• Adsorption to cell surface.• Integration to cell membrane.• Bile Salt Hydrolase (BSH) activity- prevents bile salt reabsorption.
• Cholesterol esterase activity-conversion to coprostanol.
Mechanisms of cholesterol reduction by Lactobacillus plantarum LPLDL®
AimsInvestigate the relevance of the above described mechanisms to thecholesterol lowering activity of LPLDL
Ò.
MethodsTo investigate the ability of LPLDL
Ò of passively removing cholesterolthrough adsorption on its cell membrane, pure cultures were carriedout in the presence and absence of cholesterol. Samples wereanalysed using scanning electron microscopy (SEM).Faecal, micro-scale, pH-controlled batch cultures (10ml) containingcholesterol-enriched growth media, were carried out in the presenceof LPLDL
Ò . Culture supernatants were obtained at 0, 10 and 24h for:• Bile Salt Hydrolase (BSH) activity determination.• Cholesterol and coprostanol concentrations were determinedusing GC-FID.
A double blind, placebo controlled, randomized study was carried outin normal to mildly hypercholesterolemic adults to confirmcholesterol reducing efficacy in vivo.
Results
Adsorption of cholesterol on LPLDLÒ cell surface: SEM of LPLDL
Ògrownin the absence (A) and presence of cholesterol (B) following washingin PBS.
A B
BSH activity in pH-controlled faecal batchcultures: significantincrease in bile salthydrolysis at 8 and 24hwith significantly higherpreference for glycolate.
0
50
100
150
200
250
300
350
Control Control LPLDL® Control LPLDL®
0h 8h 24h
Activ
ity u
nits
/ml
Glycholate Glycodeoxycholate Taurocholate Taurodeoxycholate
0
5
10
15
20
25
30
35
Control Control LPLDL® Control LPLDL®
0h 8h 24h
% c
hole
ster
ol re
mov
al
Cholesterol reducing activity in pH-controlled faecal batchcultures: significant increase in cholesterol reduction at 8 and24h in cultures where LPLDL
Òwas present.
Coprosanol formation in pH-controlled faecal batchcultures: Conversion ofcholesterol to coprostanol inthe presence of LPLDL
Ò after 8and 24h fermentation. Onceformed, coprostanol isexcreted in faeces in vivo.
0 h
8 h
24 h
Impact of LPLDL® intake on serum lipid profiles in vivo:
statistically significant reduction in systolic blood pressure, LDLand total cholesterol and an increase in HDL cholesterol innormal to mildly hypercholesterolemic adults
Study HighlightsOur findings suggest that LPLDL
Ò can mediate cholesterolreduction by three mechanisms:I. Adsorption
II. BSH activityIII. Enzymatic conversion to coprostanolThe ability of LPLDL
Ò to reduce cholesterol through severalmechanisms contributes to its enhanced efficacy in improvingserum lipid profiles in human studies.
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