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MICROFLUIDICS
Division of Technology Transfer
Licensing and Research Collaboration
Sample Loading And Injection
SubatmosphericPressure Chamber
Electro-OsmoticPump
Electro-PneumaticDistributor
Small Volume Transport
MicrofluidicDevice
Micro-scale Handling System
Microfluidic Technologies Available for Licensing
Microscale Fluid Handling System A solution for conducting microscale
reactions (digestion, separation etc.) and for efficiently transporting microliter to picoliter samples from a chip to an analytical device and/or a collection device.
Advantages Efficient Sample Transport
Reduces manipulations (e.g.. flushing) Reduces/eliminates problems of sample carryover
Savings Less Sample, Reagent(s), Time
Microscale Fluid Handling System The System
Multiple sample introduction methods Pressure, electrokinetic Injection
Single or multiple channel(s) & channel designs Parallel, circular/ cylindrical, trapezoidal
Multiple sample transfer methods Droplet, spray, or stream
Multiple analytical or collection devices ESI, MALDI, NMR, Fraction Collector, Chip, Multi-well Plate
Sample either liquid or gas License non-exclusively - Currently 5 licensees
Sample Loading and Injection Device A solution for a universal interface device for
transferring samples in series or parallel from sample container (e.g. multi-well plates) into channels of a multi-channel microfluidic device and/or into an analytical device that can be integrated into or separated from a microchip.
Advantages Uses standardized sample plates Variable sample volumes Reusable or disposable device
Sample Loading and Injection Device The System
Sample introduction Pressure, Electrokinetic, Vacuum, etc.
Sample trapped/digested inside loading channels High hydrodynamic resistance
Sample Elution Micro, analytical, and/or collection Device
Sample separation (optional) Electrical potential, etc
License exclusively or non-exclusively
Microchip Integrated Open-Channel Electro-osmotic Pumping System A solution to control fluid dynamics in microfluidic
device by using pump(s) to generate electro-osmotic flow or pressurized flow in the device and/or to perform sample transfer, gradient generation or fraction collection/deposition.
Advantages Easily integrated into existing microchips Its fabrication ensures high manufacturing and
operating reproducibility Simple design
Microchip Integrated Open-Channel Electro-osmotic Pumping System
The System Single or multiplexed pumps The voltage drop for operation of the electro-
osmotic micro pump may vary from a few tens to thousands of volts depending on the length of the pumping channels and desired flow rate and pressure.
License Exclusively or Non-exclusively
Electro-pneumatic Distributor for Multiplexed Myu-Tas Devices The purpose of the distributor is to supply
simultaneous electric current and pressurized gas to control individual channels of a microchip system in an assembly to use with electrospray mass spectrometry.
Advantages Maximizes sample throughput for analyzing samples
Decreases time between sample analysis Eliminates need for flushing of sample Eliminates need for washing sample probe Fast switching times Eliminates/ reduces cross contamination Decreases the number of runs Well plate samples can be used for further studies
Electro-pneumatic Distributor for Multiplexed Myu-Tas Devices
The System The distributor contains a gas channel and an electric
conductor, which supplies an electric current and pressurized gas to the system.
The electrical current forces the sample to flow in a uniform direction, which controls sample flow dynamics.
The pressure created in the system controls fluid dynamics in electric field free regions.
Each sample container/well is connected by an independent microchannel distributor to separate electrospray tip.
License exclusively or non-exclusively
Small Volume Transport A solution for moving small volumes of sample
through the capillary channels or tubing of a microfluidic device, especially long distances.
Advantages Minimal loss or dilution of sample. Minimal cross contamination between samples
Minimal loss of sample to channel walls Washes inserted between samples
Faster sample changes Multiple sample plugs injected at closely spaced intervals
Samples can be transported long distances with high speed to devices, such as an NMR.
Small Volume Transport The System
A sample/wash plug is formed between immiscible liquid plugs and immiscible liquid lining the transport channel walls.
System Characteristics Immiscible carrier – e.g. fluorocarbon Distances – yards Channel walls - fluorine rich surface
Teflon (PTFE, ETFE, FEP, NGFP)
License exclusively or non-exclusively
Subatmospheric,Variable Pressure Delivery Chamber A solution for more efficient sample
transfer from electrophoresis capillary or microchip to a mass spectrometer through an electrospray chamber by controlling pressure to allow fine control of sample flow rate from electrospray needle.
Advantages Minimal sample loss Lower evaporation of droplets Efficient desolvation Minimal power supply source needed
Subatmospheric,Variable Pressure Delivery Chamber
The System Ports for introducing gas into and withdrawing
gas from the chamber. Capillary tube(s) or microchip with a groove or
channel extending into chamber to deliver samples.
Sample moves from the sample delivery device to an electrospray tip in the chamber.
Subatmospheric pressure directs the sample flow from the electrospray tip into an analytical/collective device
License exclusively or non-exclusively
Opportunities & Facilities for Microfluidics Research
Director – Barry KargerBioanalytical instrumentation
capabilitiesState of the art facilities and
advanced methodologies for proteomics research and biomarker identification
http://www.barnett.neu.edu/
Opportunities & Facilities for Microfluidics Research
I. Mass Spectrometry:The Institute operates 16 interfaced massspectrometers; these include: • 1 Thermo Electron LTQ-FT Hybrid Linear Ion
Trap-Fourier Transform MS • 1 Lab-built, High Throughput LC MALDI-TOF MS (2 kHz Laser).• 1 Applied Biosystems AB 4700 MALDI TOF-TOF MS • 1 Micromass QTOF1 QP TOF MS• 2 Agilent 5973 GC-MS• 1 Applied Biosystems Mariner ESI orthogonal extraction TOF-MS• 1 Applied Biosystems Voyager DESTR TOF-MS• 2 Thermo Electron LTQ Linear Ion Trap • 1 Thermo Electron LCQ Deca XP 3D ion trap MS• 3 Thermo Electron LCQ Classic 3D ion trap MS• 1 Thermo Electron TSQ 700 triple QP MS• 1 Thermo Electron TSQ 7000 triple QP MS• 1 SCIEX API III PLUS triple QP MS
• 1 9-node computer cluster (18 CPU's)
reported as of 4/05.
II. Separation Instrumentation:This includes free-standing devices, listed in the following; other units are integrated both into mass spectrometers and NMR equipment.
• Beckman Proteome Lab 2D LC System• Eksigent nanoLC System• 1 Advion Nanomate 100• 1 Bio-Rad 2D gel electrophoresis system • 2 Agilent 1100 HPLC systems• 2 Agilent 1100 Cap HPLC systems• 1 Agilent G 1602 CE system• 1 Amersham Biosciences MDLC system• 1 Amersham Biosciences AKTA FPLC
• 5 Beckman CE systems• 1 Bischoff HPLC system• 3 Dionex Ultimate nanoLC systems• 2 Thermo Electron Surveyor LC sys
• 4 Agilent 1090 HPLC's• 3 Agilent 1100 LC systems• 1 Shimadzu HPLC system• 2 Thermo Electron Surveyor LC sys
III. NMR and LC-NMR:The James and Faith Waters 500 MHz NMR Facility
500 MHz NMR System (Varian Unity-Inova)• Conventional 5 mm NMR• LC NMR• High-Throughput Flow NMR• Microcoil NMR
Opportunities & Facilities for Microfluidics Research
Center for Subsurface Sensing and Imaging SystemsAcademic Partners are NU-lead, BU, RPI,
and UPRM. Strategic affiliates include MGH, Lawrence
Livermore and Idaho Nat’l Labs, Woods Hole, and Sloan-Kettering Cancer Ctr.
Industrial partners include Raytheon, ADI, Textron, Lockheed Martin, Cardiomag Imaging, Mercury, Transtech, GSSI, and Siemens.
Director - Michael Silevitch
Opportunities & Facilities for Microfluidics Research
Advanced optical instrumentation for microscopic characterization
Software algorithms for microfluidic analysis systems http://www.censsis.neu.edu/
$20 million from The Gordon Foundation
Opportunities & Facilities for Microfluidics Research
LEAP – Laboratory for electrochemical advanced power Fuel Cell Concept Laboratories Director – Sanjeev Mukerjee Advanced electrocatalyst capabilities
for proton exchange membrane systems
Micro fuel cell concept characterization and evaluation
http://www.chem.neu.edu/web/faculty/mukerjee.html
Center for advanced nanomaterials for energy conversion and storage
CANECSCANECS
Opportunities & Facilities for Microfluidics Research
Center for High-rate Nanomanufacturing Academic Partners
Northeastern University – Lead, U. Mass-Lowell, U New Hampshire, Michigan State U., Museum of Science
Director – Ahmed Busnaina
http://www.nano.neu.edu/index.html
Opportunities & Facilities for Microfluidics Research
Center for High-rate Nanomanufacturing Kostas Facility for Micro- & Nano-Fabrication of
Microfluidic Devices A core facility for the NSF Center for High Rate
Nanomanufacturing Five thousand feet of Class 10, 1000 and 10000
cleanroom facilities Capabilities for lithography, nanolithography, thin
film deposition, wet chemical processes, etching, milling and characterization
Area for undergraduate and graduate student teams working on projects with corporate partners
Anthony N. Pirri, Ph.DDirector
a.pirri@neu.edu
Susan Riley Keyes, Ph.D., J.D.Technology Transfer Executive
s.keyes@neu.edu
TBDIntellectual Property &
Contract Specialisttbd@neu.edu
Tanya Rose (part-time)Staff Assistant
t.rose@neu.edu
Felicia A JadczakTechnology Transfer Assistant
f.Jadczak@neu.edu
Volunteers, Coop MBA Students, Consultants(617) 373-8810
DIVISION of TECHNOLOGY TRANSFERSTAFF
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