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M.Sc. BIOTECHNOLOGY II SEMESTER
COURSE CODE: BTC 201 COURSE TYPE: CCC
COURSE TITLE: CODE: IMMUNOLOGY
CREDIT: 07
THEORY: 05 PRACTICAL: 02
HOURS: 135
THEORY: 90 PRACTICAL: 45
MARKS: 100
THEORY: 70 CCA: 30 PRACTICAL: 33
OBJECTIVE: The main aim of this course is to cater the students the information
regarding the body’s defence mechanism, components of defence system, genetic control
and the consequences arising due to failure of defence mechanism. The course also includes
immunotechniques for providing modern insights regarding the subject.
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Introduction, history and scope of Immune system. Types of immunity – innate,
acquired, passive and active. Clonal nature of immune response. Antigens – properties
and types. Immunogenecity and antigenecity. Factors affecting immunogenecity.
Antigenic epitopes, adjuvants, haptens, superantigens.
UN
IT-2
20
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Cells and organs of immune system: Hematopoiesis and differentiation, B and T
lymphocytes and their activation, natural killer cells, mononuclear cells, granulocytes,
dendritic cells. Lymphatic system: Primary and secondary lymphoid organs. Cell
mediated cytotoxicity: mechanism of T cell and NK cell mediated lysis and ADCC.
UN
IT-3
20 H
ou
rs Antibody structure and function isotype, allotype and idiotype. Immunoglobulin
classes and sub classes. Antigen and antibody interactions and Immuno-techniques -
ELISA, RIA, Western Blot, Immuno-blot and Immuno-fluorescent techniques. Major
histocompatibility complex, HLA. Generation of antibody diversity and complement
system.
UN
IT-4
20
Hou
rs Antigen processing and presentation, generation of humoral and cell mediated
immune responses, cytokines and their role in immune regulation, T- cell regulation,
MHC- regulation, Immunological tolerance, Hypersensitivity, Autoimmunity,
Immonosenesence.
UN
IT-5
15 H
ou
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Transplantation, Immunity to infectious agents (intracellular parasites, helimenths &
viruses,) Tumor Immunology, AIDS and other immunodeficiences. Vaccines,
Hybridoma Technology and Monoclonal Antibodies.
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1. Grouping of blood and Rh typing.
2. Latex agglutination test for rheumatoid factor and pregnancy.
3. ELISA - demonstration
4. Total RBC count, Total WBC count, Total platelet count
5. Differential WBC count & Absolute eosinophil count
6. Radial Immuno diffusion and Double diffusion.
7. Isolation and purification of IgG from serum or IgY from chicken Egg.
8. Blood smear identification of leucocytes by Giemsa stain
9. Demonstration of Phagocytosis of latex beads
10. Immuno-electrophoresis
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1. Abdul, K., Abbas, Andrew K. L., Jordan, S. P. (1998). Cellular and Molecular
Immunology. Sanders College Pub.
2. Benjamine, E., Cocoi., Sunshine. (2000). Immunology 4 th edition- Wiley- Liss.
Publ. NY.
3. Male D., Champion B. Cooke A. and Owen M. (1991) Advanced Immunology
4. Richard A. Goldsby, Thomas J. Kindt and Barbara A. (2005) Kuby
Immunology Osborne 6 Ed. Edition
5. Roitt, I.M. (1998). Essential Immunology, ELBS, Blackwell Scientific
Publishers, London.
6. Tizard I.R. (1995). Immunology, 4thedition, Saunder College Pub.
M.Sc. BIOTECHNOLOGY II SEMESTER
COURSE CODE: BTC 202 COURSE TYPE: CCC
COURSE TITLE: CODE: GENETICS AND PLANT BREEDING
CREDIT: 07
THEORY: 05 PRACTICAL: 02
HOURS: 135
THEORY: 90 PRACTICAL: 45
MARKS: 100
THEORY: 70 CCA: 30 PRACTICAL: 33
OBJECTIVE: The ever increasing population is posing major problems in front of
developing nations. Fulfillment of food requirement of all individuals is one such
challenging problem. Biotechnology offers various options in which this problem can be
dealt with. In this regards this course aims to impart basic knowledge of genetic principles
and possible ways in which these principles could be utilized in plant breeding for
production of improved varieties.
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15
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Mendel’s laws. Non Mendelian Inheritance/Medelian deviation; Gene interactions,
Incomplete dominance, Co-dominance and epistatic, Extra chromosomal inheritance,
Lethal gene, Multiple alleles. Sex determination, Chromosomal theory of inheritance.
Linkage and crossing over.
UN
IT-2
20
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Genetic mutation. Structural and numerical variations of chromosomes. DNA-based
markers; RFLP, RAPD, AFLP, CAPs, SSR, SCAR and SNPs etc. Gene tagging and
QTL mapping, mapping populations (F2s, back crosses, RILs, NILs and DH). Marker-
assisted selection and molecular breeding for qualitative and quantitative traits. Gene
pyramiding.
UN
IT-3
15 H
ou
rs DNA and RNA as genetic material. Structure of DNA different types of RNAs and
their roles; differences between DNA and RNA. Organization of DNA in
chromosomes. Genetic code, Nature, structure and replication of the genetic material.
DNA Transcription, translation and its regulation.
UN
IT-4
20
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rs Introduction, History and Objectives of plant breeding, Genetic basis of breeding self-
and cross - pollinated crops. Heritability and genetic advance, genotype environment
interaction; General and specific combining ability; Types of gene actions and
implications in plant breeding. Self-incompatibility and male sterility in crop plants.
UN
IT-5
20 H
ou
rs Pure line theory, pure line selection and mass selection methods. Population
improvement. Polyploidy in Plant Breeding. Breeding for quality. Hybrid breeding -
genetical and physiological basis of heterosis and inbreeding, Transgressive breeding.
Mutation breeding; Breeding for abiotic and biotic stresses
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1. Laboratory exercises in probability and chi-square
2. Demonstration of genetic principles using laboratory organisms
3. Chromosome mapping using three point test cross
4. Induction and detection of mutations through genetic tests.
5. Floral biology in self and cross pollinated species
6. Selfing and crossing techniques
7. Selection of breeding population
8. Selection methods in segregating populations and evaluation of breeding
material
9. Analysis of variance (ANOVA)
10. Estimation of heritability and genetic advance
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1. Chopra VL. (2001) Breeding Field Crops. Oxford & IBH
2. Chopra VL. (2004) Plant Breeding. Oxford & IBH.
3. Gupta SK. (2005) Practical Plant Breeding. Agribios.
4. Pohlman JM & Bothakur DN. (1972) Breeding Asian Field Crops. Oxford &
IBH.
5. Roy D. (2003) Plant Breeding, Analysis and Exploitation of Variation. Narosa
Publ.House.
6. Sharma JR. (2001) Principles and Practice of Plant Breeding. Tata McGraw-
Hill.
7. Simmonds NW. (1990) Principles of Crop Improvement. English Language
Book Society.
8. Singh BD. (2006) Plant Breeding. Kalyani.
9. Singh P. (2002). Objective Genetics and Plant Breeding. Kalyani.
10. Singh S & Pawar IS. (2006). Genetic Bases and Methods of Plant Breeding.
CBS.
M.Sc. BIOTECHNOLOGY II SEMESTER
COURSE CODE: BTC 203 COURSE TYPE: CCC
COURSE TITLE: GENETIC ENGINEERING & r-DNA TECHNOLOGY
CREDIT: 07
THEORY: 05 PRACTICAL: 02
HOURS: 135
THEORY: 90 PRACTICAL: 45
MARKS: 100
THEORY: 70 CCA: 30 PRACTICAL: 34
OBJECTIVE: Genetic-engineering has given new dimensions to scientific research. It has
made possible the generation of novel genetic combination within the living system, with its
applications in countless fields such as Agriculture, Medicine, Industries, Environment etc.
in this respect the paper aims at imparting knowledge regarding the procedures, tools,
guidelines and applications of gene manipulation.
UN
IT-1
18
Hou
rs Basic principles and scopes of genetic engineering. Basic tools: Restriction
endonucleases types and uses. DNA modifying enzymes: Nucleases, Polymerases,
Phosphatases and DNA ligases. Gene cloning vectors: Plasmids, Bacteriophages,
Phagemids, Cosmids, Artificial chromosomes.
UN
IT-2
18 H
ou
rs Vector Engineering and introduction of recombinant DNA into prokaryotic and
eukaryotic systems. Homo polymer tailing, cohesive and blunt end ligation, adaptors,
linkers. Recombinant screening and selection – markers, nucleic acid hybridizations:
colony, plaque, dot blot, southern and northern. Construction of genomic and c-DNA
libraries.
UN
IT1
5
18 H
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Strategies of gene delivery, Gene gun, Microenjection. In vitro translation, gene
regulation and expression in prokaryotic and eukaryotic. Production of protein drugs
for clinical trial. Chromosome engineering, Targeted gene replacement, gene editing.
UN
IT-4
18
Hou
rs Polymerase chain reaction introduction types and applications. S1 Mapping.
Restriction mapping and Chromosome walking. Molecular markers viz; RFLP,
RAPD, AFLP and SNPs in genome analysis. Application in forensic, Disease
prognosis, Genetic counseling, Pedigree analysis, Taxonomy and biodiversity.
UN
IT-5
18
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rs Transgenic and gene Knockout technologies, Gene therapy, Vectors and gene
delivery, Gene replacement/augmentation, Processing of Recombinant proteins, Intra
cellular periplasmic and extra cellular expression of protein. Purification and
refolding. Characterization of recombinant proteins. Stabilization of proteins. Phage
display system.
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1. Isolation of genomic DNA from microbial, plant and animal sources.
2. Isolation of plasmid DNA.
3. Estimation of DNA using diphenylamine reagent and by UV
spectrophotometer.
4. Isolation of RNA from yeast.
5. Estimation of RNA using orcinol reagent and by UV spectrophotometry.
6. Digestion of plasmid DNA with restriction endonucleases.
7. Separation of DNA fragments by Agarose gel electrophoresis.
8. Elution of DNA from agarose gels.
9. Ligation of DNA fragments.
10. Amplification of DNA by PCR.
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1. Anthony JF Griffiths, William M Gelbart, Jeffrey H Miller, and Richard C.
Lewontin, (1999) Modern Genetic Analysis (1 st Ed.)W. H. Freeman
Publishers.NY.
2. Benjamin Lewis, (2004) Genes VIII (3rd
Ed.) Oxford University & Cell Press,
NY.
3. Ernst-L Winnacker, (2003) From Genes to Clones: Introduction to Gene
Technology WILEY-VCH Verlag GmbH, Weinheim, Germany Reprinted by
Panima Publishing Corporation, New Delhi.
4. Nicholl D.S.T. (2000) Introduction to Genetic Engineering Cambridge
(3rdEd.) University press. UK.
5. Old R.W., Primrose S.B. (1996) Principles of gene manipulation - An
introduction to genetic engineering (5thEd.), Blackwell Scientific Publications,
6. S. B. Primrose, Richard M. Twyman, (2006) Principles of gene manipulation
and genomics (7 th Ed.) John Wiley & Sons publishers.
7. Sambrook J et al. (2000) Molecular cloning Volumes I, II and III. Cold Spring
Harbor laboratory Press, New York, USA.
M.Sc. BIOTECHNOLOGY II SEMESTER
COURSE CODE: BTC 221 COURSE TYPE: PRJ/FST/EST
COURSE TITLE: SOCIAL OUTREACH AND SKILL DEVELOPMENT
CREDIT: 06
THEORY: 06
HOURS: 90
THEORY: 90
MARKS: 100
THEORY: 70 CCA: 30
Note for Students:
1. During the 2nd
semester, all students will have to go for Educational Study Tour/Field
Study Tour (EST/FST) under the supervision of Professor/ Associate Professor/
Assistant Professor of Dept. of Biotechnology, Sarguja University, Ambikapur,
Chhattisgarh and finally will have to submit report not more than 40 pages on his/her
assignment.
2. Report of Educational Study Tour/Field Study Tour (EST/FST) will be evaluated by
the board/committee constituted by the Head, Dept. of Biotechnology, Sarguja
University, Ambikapur, at the Dept. of Biotechnology Sarguja University,
Ambikapur.
M.Sc. BIOTECHNOLOGY II SEMESTER
COURSE CODE: BTC B01 COURSE TYPE: ECC/CB
COURSE TITLE: ENVIRONMENTAL AND FOREST LAWS
CREDIT: 06
THEORY: 06
HOURS: 90
THEORY: 90
MARKS: 100
THEORY: 70 CCA: 30
OBJECTIVE:
Understands the concept and place of research in concerned subject
Gets acquainted with various resources for research
Becomes familiar with various tools of research
Gets conversant with sampling techniques, methods of research and techniques of
analysis of data
Achieves skills in various research writings
Gets acquainted with computer Fundamentals and Office Software Package.
UN
IT-1
18 H
ou
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Evolution of Forest and Wild Life Laws:
a) Importance of Forest and Wildlife
b) Evolution of Forest and Wild Life Laws
c) Forest Policy during British Regime
d) Forest Policies after Independence.
e) Methods of Forest and Wildlife Conservation.
UN
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Forest Protection and Law
a) Indian Forest Act, 1927,
b) Forest Conservation Act, 1980 & Rules therein
c) Rights of Forest Dwellers and Tribal
d) The Forest Rights Act, 2006
e) National Forest Policy 1988
UN
IT-3
18 H
ou
rs Wildlife Protection and Law
a) Wild Life Protection Act, 1972
b) Wild Life Conservation strategy and Projects
c) The National Zoo Policy
UN
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18
Hou
rs
Chapter – Basic Concepts
a) Meaning and definition of environment.
b) Multidisciplinary nature of environment
c) Concept of ecology and ecosystem
d) Importance of environment
e) Meaning and types of environmental pollution.
f) Factors responsible for environmental degradation.
Chapter– Introduction to Legal System
a) Acts, Rules, Policies, Notification, circulars etc
b) Constitutional provisions on Environment Protection
c) Judicial review, precedents
d) Writ petitions, PIL and Judicial Activism
Chapter – Legislative Framework for Pollution Control Laws
a) Air Pollution and Law.
b) Water Pollution and Law.
c) Noise Pollution and Law.
UN
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18
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Chapter- Legislative Framework for Environment Protection
a) Environment Protection Act & rules there under
b) Hazardous Waste and Law
c) Principles of Strict and absolute Liability.
d) Public Liability Insurance Act
e) Environment Impact Assessment Regulations in India
Chapter – Environmental Constitutionalism
a) Fundamental Rights and Environment
i) Right to Equality ……….Article 14
ii) Right to Information ……Article 19
iii) Right to Life …………..Article 21
iv) Freedom of Trade vis-à-vis Environment Protection
b) The Forty-Second Amendment Act
c) Directive Principles of State Policy & Fundamental Duties
d) Judicial Activism and PIL
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1. Bharucha, Erach. (2005) Text Book of Environmental Studies. Hyderabad :
University Press (India) Private limited,
2. Doabia, T. S. (2005) Environmental and Pollution Laws in India. New Delhi:
Wadhwa and Company,
3. Environmental Pollution by Asthana and Asthana, S,Chand Publication
4. Environmental Science by Dr. S.R.Myneni, Asia law House
5. Joseph, Benny (2006)Environmental Studies, New Delhi: Tata McGraw-Hill
Publishing Company Limited,
6. Khan. I. A, (2002) Text Book of Environmental Laws. Allahabad: Central Law
Agency,
7. Leelakrishnan, P. (2006) Environmental Law Case Book. 2nd
Edition. New
Delhi: LexisNexis Butterworths,
8. Shastri, S. C (2006) Human Rights, Development and Environmental Law, An
Anthology. Jaipur: Bharat law Publications,
9. Gurdip Singh, Environmental Law in India (2005) Macmillan.
10. Shyam Diwan and Armin Rosencranz, Environmental Law and Policy in India
–
11. Cases, Materials and Statutes (2nd
ed., 2001) Oxford University Press.
JOURNALS :-
Journal of Indian Law Institute, ILI New Delhi.
Journal of Environmental Law, NLSIU, Bangalore.
MAGAZINES :-
Economical and Political Weekly
Down to Earth.
M.Sc. BIOTECHNOLOGY II SEMESTER
COURSE CODE: BTC B02 COURSE TYPE: ECC/CB
COURSE TITLE: ENZYMOLOGY & ENZYME TECHNOLOGY
CREDIT: 06
THEORY: 06
HOURS: 90
THEORY: 90
MARKS: 100
THEORY: 70 CCA: 30
OBJECTIVE: Enzymes are the bio-catalysts involved in numerous bio-chemical reactions
within the cell. More importantly enzyme activity is also observed in-vitro under
appropriate conditions and hence enzymes can also be utilized outside the cellular
environment as in industrial fermentation, research purposes etc. in this regards, this
paper aims to impart the students the basic knowledge concerning enzyme functioning,
kinetics, industrial application to help students pursue their career in this field in future.
UN
IT-1
18
Hou
rs Introduction to Enzymes, enzyme nomenclature, enzyme commission numbers, and
classification of enzymes. Isolation and purification of enzymes, preparation of
purification chart, Enzyme activity, Specific activity and turn over number, Marker
enzymes.
UN
IT-2
18 H
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Enzyme Kinetics: Steady state, pre-steady state, equilibrium, kinetics, Michaelis and
Menten Equation and its derivation, Different methods to calculate the Km and Vmax
and their significance.
UN
IT-3
18
Hou
rs Factor affecting enzyme activity and catalysis: pH, substrate and enzyme
concentration, temperature, coenzyme and cofactors, Mechanism of action of enzymes
involving two/more substrates. Role of metal ions in enzyme catalysis. Enzyme
inhibition, different types of inhibitors and activators.
UN
IT-4
18 H
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Structure and function of enzymes: Lysozyme, chymotrypsin, DNA polymerase,
RNase, proteases. Enzyme regulation and control of their activity. Introduction to
allosteric enzymes and isozymes.
UN
IT-5
18 H
ou
rs Enzyme Technology: Immobilization of enzymes, whole cell immobilization and their
application, commercial production of enzymes, RNA-catalysis, Catalytic antibodies -
abzymes, Protein and Enzyme engineering: Design and construction of novel
enzymes.
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1. A textbook of Enzyme Biotechnology By Alan Wiseman.
2. Advances in Enzymology: v. 47 (Hardcover) By Alton Meister. John Wiley and
Sons Inc.
1. Biochemistry: Biomolecules, Mechanisms of Enzyme Action and Metabolism
Vol 1 (Hardcover) By D Voet. John Wiley
3. Enzymes By M Dixon and EC Webb. EC Longmans, London.
4. Enzymes: Biochemistry, Biotechnology and Clinical Chemistry By Trevor
Palmer.
5. Fundamentals of Enzymology: Cell and Molecular Biology of Catalytic
Proteins (Paperback)By Nicholas C. Price and Lewis Stevens. Oxford
University Press.
6. Lehninger Principles of Biochemistry 4th Ed By David L. Nelson and Michael
M. Cox,WH Freeman and Company.
7. Nature of Enzymology By RL Foster
8. Principles of Biochemistry (Hardcover)By Geoffrey Zubay. Publisher:
McGraw Hill College.
M.Sc. BIOTECHNOLOGY II SEMESTER
COURSE CODE: BTC B03 COURSE TYPE: ECC/CB
COURSE TITLE: MOLECULAR BIOTECHNOLOGY
CREDIT: 06
THEORY: 06
HOURS: 90
THEORY: 90
MARKS: 100
THEORY: 70 CCA: 30
OBJECTIVE: The main objective of this paper is to impart the students basic knowledge
of advanced biotechnological methods, their principles and their possible uses in modern
biotechnological research.
UN
IT-1
18 H
ou
rs Proteomics techniques: Techniques used in gene detection and expression studies:
Southern hybridization, Northern hybridization, western hybridization, PCR and RT-
PCR Peptide sequencing and synthesis: principles and strategies for protein
sequencing. Design of primers from ammo acids sequences
UN
IT-2
18 H
ou
rs DNA- protein interaction techniques: Gel mobility shift assay, DNA-protein cross-
linking assay, Dnase I foot printing and Sl nuclease mapping. Protein- protein
interactions: chemical cross-linking. Yeast-2-hybid, Yeast-3-hybid and their various
versions. Principles and applications.
UN
IT-3
18
Hou
rs
Reporter genes: Chloramphenicol acetyl transferase (cat), neomycin phosphoryl
transferase II (nptII), Luciferase, β- galactosidese etc. and their applications in
expression kinetics and promoter probing studies.
UN
IT-4
18
Hou
rs
Protein folding: Protein folding and the roles of Molecular chaperones. Mechanism
and relevance to biotechnology. Assisted protein folding , In-vitro protein folding.
UN
IT-5
18
Hou
rs Drugs design: Drug design and various approaches: by blocking enzyme activity,
Inhibitor for Dihydroxyfolate reducase (DHFR), Renin. HIV reverse transcriptase etc
Drug design by blocking hormone receptors, propanolol for norepinephrine and
epinephrine etc, and drug design by inhibiting nucleic acid synthesis using antisense
RNA technology.
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1. Nicholl, An Introduction to Genetic Engg. - 2 ed, Cambridge
2. Primrose, Principles of Gene Manipulation - 6 ed, Blackwell
3. Winnacker, From Genes to Clones, Panima
4. Primrose, Principle of Gene Manipulation, Blackwell
5. Griffiths, Intro. to Genetic Analysis - 8 ed, Freeman Pub.
6. Maxine singer-berg, Genes – Genomes, Uni. Sci. Book
7. T.A.Brown, Gene Clonning - DNA Analysis, Blackwell
8. John Witkowski, Recombinant DNA, Scientific American
9. Piramal, Molecular Biotechnology, Dominant Pub.
10. Christoph W. Sensen , Essentials Of Genomics and Bioinformatics, Wiley-
VCH.
11. David Bowtell and Joseph Sambrook, DNA Microarrays, CSHL Press
M.Sc. BIOTECHNOLOGY II SEMESTER
COURSE CODE: BTC B04 COURSE TYPE: ECC/CB
COURSE TITLE: HUMAN MOLECULAR GENETICS
CREDIT: 06
THEORY: 06
HOURS: 90
THEORY: 90
MARKS: 100
THEORY: 70 CCA : 30
OBJECTIVE: The main objective of this paper is to cater the students the information
regarding advanced molecular techniques used in analysis of human genetic disorders so
that the students will develop an insight regarding molecular mechanism of genetic
disorders and develop interest in the direction of their amelioration in future.
UN
IT-1
20
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Genetic mapping of Mendelian traits: Identifying recombinants and non-
recombinants in pedigrees, Genetic and physical map distances, Genetic markers.
Two-point mapping- LOD score analysis, Multipoint mapping, Homozygosity
mapping
UN
IT-2
17
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Genetic mapping of complex traits: Difficulties in mapping complex traits, Allele
sharing methods- Affected sib pair analysis, Allelic association, Linkage
disequilibrium mapping, Transmission disequilibrium test.
UN
IT-3
20 H
ou
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Physical mapping methods, Low resolution mapping- Cell hybrids, mini- and
microcells, synteny of genes, Radiation hybrid mapping, Assembly of clone contigs,
Identifying genes in cloned DNA, Integration of cytogenetic, genetic and physical
map
UN
IT-4
15
Hou
rs Molecular pathology, Nomenclature of mutations and their databases, Loss-of-
function and gain-of-function mutations in diseases, Instability of the human genome:
Pathogenicity associated with repeat sequences.
UN
IT-5
18 H
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DNA testing, Direct testing, Screening for unknown mutations, Detection of known
mutations, Indirect tests – gene tracking, DNA profiling: establishing identity and
relationships, Population screening - ethics, organization and advantages.
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1 Davies Human Genetic Disease Analysis IRL (1993)
2. Haines & Pericak (1998) Approaches to Gene Mapping in Complex Human
Diseases, Wiley
3. Nussbaum et al (2001) Genetics in Medicine Saunders
4. Pasternak (2000) An Introduction to Molecular Human Genetics, Fritzgerald
5. Rimoin et al (2002) Principles & Practice of Medical Genetics, Vol I-III
Churchill
6. Strachan & Read (1999) Human Molecular Genetics, Wiley
7. Sudbery (2002) Human Molecular Genetics, Prentice-Hall
M.Sc. BIOTECHNOLOGY II SEMESTER
COURSE CODE: BTC B05 COURSE TYPE: ECC/CB
COURSE TITLE: BIOETHICS & BIOTECHNOLOGY MANAGEMENT
CREDIT: 06
THEORY: 05 PRACTICAL: 00
HOURS: 90
THEORY: 90 PRACTICAL: 00
MARKS: 100
THEORY: 70 CCA: 30
Objectives: This paper provides information on ethical issues involved in biotechnology
experiments. This also addresses on Biosafety aspects in Biotechnology and intellectual
property rights. The main objectives of this course are; 1) To enable the students to know
about the legal issues affecting the biotechnology research and how to establish ownership
of a novel finding. 2) The students will be able to understand the legal aspects involved in
the biotechnology research.
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15
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Bioethics: Ethical issues related to biotechnology and biomedical research and their
impact to living system, Public education of the processes of biotechnology involved
in generating new forms of life for informed decision making.
UN
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15
Ho
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Socioeconomic Impacts of Biotechnology: Beneficial applications and development of
research focus to the need of the poor, identification of directions for yield effect in
agriculture, bioremediation and environmental impacts of release of GMOs, social
issues to biotechnology.
UN
IT-3
15 H
ou
rs
Introduction – Meaning – nature and characteristics of Management, Management V/s
Administration, Roles of Management, Levels of Management. Evolution of
management thought: early, contemporary and modern.
UN
IT-4
15 H
ou
rs Nature, purpose and importance of planning process. Types of plans. Decision
making. Importance of planning – steps in planning & planning premises.
Nature and purpose of organization. Principles of organization, Types of organization.
Meaning and nature of directing.
UN
IT-5
15 H
ou
rs Structure of a Biotechnology Company. Start-up of Biotechnology Company, New
Product Development. Market Research. Sales & Marketing Principles. Intellectual
Property Principles in Biotechnology. Health Care Overview and Role of Government
in Biotechnology.
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1. Fleming, D.A., Hunt, D.L., (2000) Biological safety Principles and practices.
ASM Press.
2. P.C.Tripathi, P.N. Reddy: Principles of Management –– Tata McGraw Hill,
3. Poornima.M.Charantimath (2006) Entrepreneurship Development –– Small
Business Enterprises – Pearson Education (2 & 4).
4. S.S.Khanka: Entrepreneurship Development –– S.Chand & Co.
5. Sassaon A. (1988) Biotechnologies and development. UNESCO Publications.
6. Sateesh, M.K. (2008) Bioethics & Biosaftey, IK Publishers.
7. Thomas, J.A. and Fuch, R.L. (2002) Biotechnology and Safety Assessment.
Academic Press.
8. Vasant Desai: Dynamics of Entrepreneurial Development & Management–
Himalaya Publishing House
M.Sc. BIOTECHNOLOGY II SEMESTER
COURSE CODE: BTC B06 COURSE TYPE: ECC/CB
COURSE TITLE: SCIENCE & TECHNOLOGY FOR RURAL DEVELOPMENT
CREDIT: 06
THEORY: 06
HOURS: 90
THEORY: 90
MARKS: 100
THEORY: 70 CCA: 30
OBJECTIVE: The real India lives in the Indian villages. The main occupation of villagers
is agriculture and animal and related works. Till the date not much change is evidenced in
the village scenario. The problem of waste management, hygiene, balanced food etc and
yet more still prevails in our villages, in this regards this paper aims to give the students
insights of some simple yet efficient techniques to deal with such problems and comfort the
life , raise the life standards and present new options for the economic growth.
UN
IT-1
20
Ho
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Microbial inoculants- Selection & establishment of nitrogen fixing bacteria.Production
of Rhizobium, Azotobacter, Azospirilla, cyanobacteria and other nitrogen fixing
bacterial cultures. Quality control of bio inoculants; Phosphate solubilising bacteria;
mycorrhiza; plant growth promoting rhizobacteria (PGPR); Biocontrol microbial
inoculants.
UN
IT-2
20
Ho
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Role of microorganisms for biomonitoring of various quality-parameters related to
water and wastewater - Indicator organisms, single species laboratory bioassays and
biosensors. Brief introduction to various stages of wastewater treatment: Primary,
secondary and tertiary treatment. Control of pathogens in water and wastewater. Use
of microorganisms for removal of various toxins and metallic ions from wastewater.
UN
IT-3
20 H
ou
rs Vermiculture: definition, scope and importance - common species for culture, culture
methods, wormery breeding techniques - indoor and outdoor cultures - monoculture
and polyculture - relative merits and demerits. Vermin-composting, use of
vermicastings in organic farming / horticulture - earthworms for management of
biomedical solid wastes - feed / bait for capture / culture fisheries - forest regeneration
UN
IT-4
15 H
ou
rs
Single cell protein: microorganisms used; raw material used as substrate; condition for
growth and production; nutritive value and uses of SCP. Baker’s yeast; Production of
probiotic biomass; and mold cultures. Mushroom production: cultivation of different
types of mushroom; edible mushroom; diseases of mushrooms therapeutic value of an
edible mushroom; production of pectin and microbial conversion of woody biomass. U
NIT
-5
20 H
ou
rs
Mass cultivation of cyanobacteria under outdoor and indoor conditions. Cyanobacteria
as a source of fine chemicals, polysaccharides, bioactive molecules, pigments,
antioxidants, lipids and polyunsaturated fatty acids. Cyanobacteria as biofertilizer for
paddy cultivation. Hydrogen production by cyanobacteria: Mechanism, progress and
prospects. Cyanobacterial and algal fuels; Fine chemicals (restriction enzymes etc)
and nutraceuticals from algae
SU
GG
ES
TE
D
RE
AD
ING
S
1. Casida, Industrial microbiology-, L.E. New age international Ltd, Publishers.
New Delhi:
2. Edwards, C.A. and Bohlen, P. J (1996) ecology of earthworms - 3rd Edition,
Chapman and hall.
3. Frazier, Food microbiology. W.C. Tata McGraw Hill.
4. Jsmail, S.A., (1970) Vermicology, The biology of earthworms, Orient
Longman, London.
5. L. M. Prescott, J. P. Harley and D. A. Klein. Microbiology-, McGraw Hill
6. Lee, K.E., (1985) Earthworms - Their ecology and Relationship with Soil and
Land use, Academic Press, Sydney.
7. N. J. Pelczar, S. Chand, R. Krieg. Microbiology- Tata McGraw Hill
8. Robert A Andersen. (2005) Algal Culturing Techniques. Academic Press.
9. Sultan Ahmed Ismail, (2005) The Earthworm Book, Second Revised Edition. Mother
India Press, Goa.
Recommended