Primary Immunodeficiency Preeda Sangacharoenkit M.D. Division of Allergy and Immunology Department...

Primary Immunodeficiency

Preeda Sangacharoenkit M.D.Division of Allergy and Immunology

Department of PediatricsBangkok Metropolitan Administrative

Medical Colleague

Cooper et al. American Academy of Family Physicians November 2003.

Cooper et al. American Academy of Family Physicians November 2003.

Rosenzweig and Holland. J Allergy Ciln Immunol April 2004.

Evaluation of T-cell function

• Quantitative evaluation

: T-cell subpopulation

• Qualitative evaluation

: Delayed-type hypersensitivity (DTH) skin testing

Fleisher and Oliveira. J Allergy Clin Immunol August 2004.

Evaluation of phagocytic function

• The nitroblue tetrazolium test (NBT)

• Fluorescent cytometry using the dihydrorhodamine-123 (DHR) probe

Segal and Holland. Pediatric Clinics of North America December 2000.

Roos et al. Microbes and Infection 5 (2003)

Panel D shows the results of the nitroblue tetrazolium test in normal neutrophils: phagocytosis results in dark-blue staining of the cytoplasm. Panel E shows the results of the nitroblue tetrazolium test in neutrophils from a patient with chronic granulomatous disease: there is no phagocytosis and thus no dark-blue cytoplasmic staining.

Lekstrom-Himes and Gallin. NEJM December 2000.

Jirapongsananuruk et al. J Allergy Clin Immunol February 2003.