Regional Breeding for Wheat Stripe Rust Resistance in the Eastern United States David Marshall Plant...

Regional Breeding for Wheat Stripe Rust

Resistance in the Eastern United States

David Marshall

Plant Science Research Unit

Raleigh, NC

The U.S. has the most cost-The U.S. has the most cost-effective and plentiful food effective and plentiful food production and supply production and supply system in the world.system in the world.

Wheat Stripe Rust

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19761978

19801981

19831984

19871990

19992000

20012002

20032004

20052006

IdahoOregon

WashingtonCalifornia

KansasColoradoNebraskaLouisianaArkansasEpidemic Year

% Loss

Stripe Rust Epidemic Years and the States with Greater than 1% Yield Loss from 1976 to 2004.

Objectives:

Identify stripe rust resistance/susceptibility in Eastern U.S. wheat cultivars, elite lines, and other germplasm.

Identify the gene or genes involved resistance.

Introgress stripe rust resistance into elite lines having regional/local adaptation.

Distribute populations and fixed lines to cooperators.

Protocol:

Established the Uniform Eastern Wheat Stripe Rust Nursery. In 2005-06, had up to 20 entries from State breeding programs in FL, GA, NC, VA, MD, NY, MI, MO, IL, IN, KY, AR, and LA; private breeding programs – Westbred, AgriPro-Coker, and Genesis; and USDA/ARS.

Seedling screening in Raleigh, NC.

Field screening in Plains and Griffin, GA; Kinston, NC; Baton Rouge and Winnsboro, LA; Fayetteville, AR; San Antonio, TX, Pullman and Mt. Vernon, WA.

IT %

Designation Pedigree Contributor Source Mean Mean

TERRAL LA841

GA8665-D4/GA85238-C3 Harrison Louisiana 0.00 0.00

GA961567-4A35

Jackson/2*881130 Johnson Georgia 0.00 0.00

GA97531-5A37

891138/Kinsco//PIO 2628 Johnson Georgia 0.00 0.00

LA99042E-62-B

Ducula1(H2O)/Mason,F1//PIO26R61 Harrison Louisiana 0.75 0.03

LA96140BUA70-2

VA88-52-69/TX89D6435//C9134,F1/3/Mason Harrison Louisiana 0.75 0.03

GA951231-4E26

881130/C9134 Johnson Georgia 0.25 0.03

NC03-7222 L910568/NCV93-1007//LA854111 Murphy North Carolina

0.25 0.03

MO 050146 MO 94-046/Pioneer 2552 McKendry Missouri 0.25 0.03

IL87-2834-1 Tyler / Howell Kolb Illinois 0.75 0.03

992133A2-1-2

Patterson/F201R//INW0101/4/Patton/3/Clark*4/Ning7840//Foster

Ohm Indiana 0.25 0.03

Identified 102 lines with resistance better than the "resistant" check (Pioneer 26R61). Infection types less than '3' and severities less than 18%.

Identified adult-plant resistance in at least 8 lines:

LA95171CA58-3-2-C CK9134/PIO2684,F1//90239A29-1-X(CL850643/PIO2548//C9877)

97C-0554-01 VA94-54-549/Roane//Kristy

B010973 L880085/XW502

Coker 9511 L881060/L880436

IL87-2834-1 Tyler / Howell

IL02-5030 P88288C1-1-6-1-2-8 / 96-6472

IL03-18438 97-3574 / 95-4162

INW0412 Huapei 57-2/Patterson

Several lines had combined stripe rust, leaf rust and powdery mildew resistance.

FL91226A-X4 Star'S'(IBW179)/C9105//F302/P2550,F5IDEO

FL98031-D15-E4 8952-B-7/CM98322-0M-2Y,F1//P26R61

LA95171CA58-3-2-C CK9134/PIO2684,F1//90239A29-1-X(CL850643/PIO2548//C9877)

LA98094-BUB-58-5 90185B24-4-3/87167D8-2

GA 96229-3A41 881130/*2 881582

GA961567-4A35 Jackson/2*881130

GA981621-5E34 AGS 2485/PIO 26R61

GA98249G1-5E56 881130/ 2*901146

GA011450DH2-5A45 USG 3592/PIO 26R61

ARS03-3849 TX85-264*2/TTCC512 (T.cylindricum) (=WX02ARS149-8)

VA01W-205 PION2684/93-54-185 (WLR/3/MSY*3/BALKAN//SAL)// POCAHONTAS,F11

B990133 L890145/LX 8728D

INW0412 Huapei 57-2/Patterson

99840C4-8-4 961331/INW0102//F201R/Foster

E01-1053 U90-1A//ZX90-2C1/P2580

Regional Breeding scheme for introducing resistant sources:

A. Backcross-Bulk (minor genes)

1. Several resistant sources x Adapted type

2. Backcross to Adapted type

3. Select in BC1 through F3 for low terminal severities

B. Accelerated Population Development for major genes

1. Resistant source x Adapted type (crosses made in February)

2. F1 planted in NZ in May, F2 in NC in Nov, F3 in NZ in June, F4 in in Nov.