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This provides a comprehensive review material for RNA Biology
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RNA - Transcription, Regulation and sequencingByLakshmikirupa SDNARNAsTranscriptionSplicingRegulation examplesSequencing
Braveheart!DNA the genetic material!
DNA magnified a million times!!
Scannimg tunneling micrograph of DNA 2,000,000X98 % of DNA is junk??Central dogmaMammalian Gene collection project cDNA sequences of coding potential based on 5 end seq
FANTOM cDNA sequences of novelty of end sequences
No of lond ncRNAs >>> protein coding RNAs
Tiling arrayUpto 93 % of genome is transcribed
The Chemistry!
The meeting point of Coding and non coding RNAsTranscription
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RNAsnRNAsnoRNAscaRNArRNAtRNAlinRNAAnti-sense RNA
RNAirasiRNApiRNAsiRNAmiRNA stRNAmRNACodingNon coding
Various functions of lncRNARNA in unexpected placesImprintingDosage compensationCell cycle regulationPluripotencyRetro-transposon silencingMeiotic entryTelomere lengthChromatin state regulation
Post-transcriptional regulationPost-translational protein activity regulationProtein complexes organisationCell-cell signallingRecombination
Long non coding RNAsRegulate gene expression by various mechanismsSpecies specific less conservativeDifferentially across various tissuesZebrafish embryos- loss of function of cyrano and megamind various developmental defects HOTTIP limb formationSubset of lncRNA depletion in mouse global lineage program upregulationpromote or suppress somatic differentiation
5% of the cells weight is RNA whereas DNA is just 1%Half-life of DNA is 521 yearsSingle gram of DNA can store 700 terabytes of data on a single gram of DNA
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