Single Nanoparticle Biosensorics - Leibniz Institut

SSingle Nanoparticle Biosensorics

Thomas Schneider, Norbert Jahr, Andrea Csaki, Wolfgang Fritzsche

fritzsche@ipht-jena.de

Institute of Photonic Technology (IPHT), Nano Biophotonics Department, Jena, Germany

13.10.2010� 2�

SSingle (Metal) Nanoparticle Biosensorics�

Single (Semiconductor) Nanowire Biosensorics�

Christian Leiterer, Gerald Brönstrup, Silke Christiansen�

Single Nanoparticle Antenna

Janina Wirth, Frank Garwe, Alla Nykytenko

Single (Sem

13.10.2010 3� Interaction of Ag/Au Nanoparticles with Light

LSPR - localized surface plasmon resonance�

ensemble� single nanoparticle�

Mie 1908 Ann Phys Kreibig/Vollmer 1995 Optical Properties of Metal Clusters Vol. 25. Springer, Heidelberg

13.10.2010� 4�

Mirkin, C. A., et al. (1996). Nature 382: 607 Alivisatos, A. P., et al. (1996). Nature 382: 609�

13.10.2010�Sensoric potential of single nanoparticles� 5�

inte

nsity�

wavelength �

13.10.2010� 6�

Nanoparticles...�

Yguerabide/Yguerabide 1998 Anal Biochem�

Single particle spectroscopy�

13.10.2010� 8�

Single Nanoparticle Plasmonics

Richard Zsigmondy 1865-1929

Henry Siedentopf 1872-1940

1907�

13.10.2010� 9�

500 nm�

3

height: 105 nm + BSA� + anti-BSA�

Schneider et al. (in preparation)�

nanoparticles�

Single-particle measurements: Proteins�

13.10.2010�Sensoric potential of single nanoparticles� 10�

3 562 nm → 569 nm → 581nm

500 nm�

height: 105 nm + BSA� + anti-BSA�nanoparticles�

Schneider et al. (in preparation)�

Single-particle measurements: Proteins�

13.10.2010� 11�

Single (Metal) Nanoparticle Biosensorics�

SSingle (Semiconductor) Nanowire Biosensorics�

Christian Leiterer, Gerald Brönstrup, Silke Christiansen��

Single Nanoparticle Antenna

Janina Wirth, Frank Garwe, Alla Nykytenko

Single (Me

13.10.2010 12� Preparation of Si-Nanowire (SiNW)

13.10.2010 13� Si-NW

S. Christiansen et al.

13.10.2010 14� Si-NW in dark field (scattering) contrast

C. Leiterer

13.10.2010 16� Si-NW for electrical bioanalytics

C. Leiterer

13.10.2010 17� Microarray chip substrate & Microfluidics

T. Schüler et al. Biosensors and Bioelectronics 24 (2009) 2077–2084 /Biosensors and Bioelectronics 25 (2009) 15–21/

13.10.2010� 18�

Single (Metal) Nanoparticle Biosensorics�

Single (Semiconductor) Nanowire Biosensorics�

Christian Leiterer, Gerald Brönstrup, Silke Christiansen�

SSingle Nanoparticle Antenna

Janina Wirth, Frank Garwe, Alla Nykytenko

Single (Me

Single (Sem

13.10.2010� 19�

Wellenlänge [nm]�

Absorption�

300 400 500 600 Wellenlänge [nm]

Optical Properties of Particles

Silver (40 nm) Gold (15 nm)

Gold/Silver (core/shell)

Lase

r 53

2 nm

Au 5 nm Au 15 nm Au 60 nm Ag 60 nm

27

26

25

24

23

21

22

[°C]

Thermography

(droplets with nanparticle solution after laser irradiation)

13.10.2010� 20� Optically-induced nanolocal manipulation: Metaphase Chromosome

Medical Laser Application 20 (2005) 201, Nano Letters 7 (2007) 247-253

Human Metaphase-Chromosomes

2 µm

1 µm 1 µm

Frank Garwe, Karsten König (JenLab GmbH)

13.10.2010� 23�

before laser� after laser�

13.10.2010�Nanoparticle-labelled DNA-Origami� 24�

•  “Smiley”-like DNA-origami and sequence-specific labelled origami (12 nm Au nanoparticles) AFM-image on mica �

A. Kuzyk, A.-P. Eskelinen, P. Törmä�

13.10.2010� 25�

Acknowledgements

IPHT:

F. Garwe R. Möller G. Brönstrup

J. Popp S. Christiansen V. Deckert

R. Kretschmer M. Urban M. Sossna H. Porwol S. Schröter R. Pöhlmann

A. Csaki O. Stranik T. Schneider N. Jahr J. Wirth A. Nykkytenko F. Jahn

External collaborations:

M. Köhler (TU Ilmenau) P. Törmä (Helsinki) M. Heller (UCSD) J. Krenn (Graz) C. Biskup (FSU Jena)

former members:

A. Steinbrück A. Wolff T. Schüler G. Festag

A. Breitenstein (ScanBec/BioSolutions GmbH) J. Hainfeld (Nanoprobes Inc.) T. Asmus (Heraeus Sensor Technology GmbH)