Sotheavy Vann Ngoc Phan

Evidence that talin alternative splice variants from Ciona intestinalis have different roles in cell adhesion

Sotheavy VannNgoc Phan

• Modular cytoskeletal proteins-cell adhesions

• Talin 1 and 2 have non-redundant roles• Talin 1-focal cell adhesion and cellular motility

• Talin 2-lables F-actin stress fibers for stable adhesion assemblies

• C. Intestinalis-talin a and talin b• Why study talins?

What are Talins?

Ciona Intestinalis • Phylum Chordata• Life Cycle• Good Model Organism

http://www.google.com/url?source=imglanding&ct=img&q=http://cfb.unh.edu/phycokey/Choices/Anomalous_Items/Chordates/tunicates/tunicate_03_600x450_larvae_riley_nolan.jpg&sa=X&ei=XLiFT9ePDYie8gT15cXCCA&ved=0CA0Q8wc4FQ&usg=AFQjCNGQ0Y-9A4X0sBmrUGdJaCGDTbektg

http://upload.wikimedia.org/wikipedia/commons/thumb/f/fe/Cionaintestinalis.jpg/220px-Cionaintestinalis.jpg

Expressed Sequence Tag•Gene Discovery Tool

•EST Method• Involves the Central Dogma of genetics

•Other Methods• Searching for signal sequences• Comparing genomes

Distribution of Talin-a and Talin-b

Phylogeny

Alternative Splicing

Alternative Splicing

Immunoflourescence

Actin binding of Talin-a and Talin-b I/LWEQ modules