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User Manual – Part 1
System Hardware
Small animal scanner
inSight64, inVision128 and inVision256-TF
iThera Medical GmbH
Zielstattstrasse 13
81379 München
Germany
Small Animal Imaging System
MSOT inSight64, inVision128 and inVision256-TF
v3.0, December 11, 2014
15.3.1-M-01
MSOT inVision Series
User Manual – Part 1 – System Hardware
page 2 / 45
Table of Contents
1 Warning Symbols .............................................................................................. 5
1.1 Legend of symbols: ................................................................................................. 5
1.1.1 Warning signs .......................................................................................... 5
1.1.2 Signs advising of necessary activities ..................................................... 6
1.1.3 Safety Labels ........................................................................................... 6
1.1.3.1 Location of labels .............................................................................. 6
1.1.3.2 Label types ........................................................................................ 8
2 About this manual ............................................................................................. 9
2.1 User manual: ........................................................................................................... 9
2.2 Declaration of Conformity: ....................................................................................... 9
2.3 Operation and expert staff: ...................................................................................... 9
2.4 Product liability: ....................................................................................................... 9
2.5 General guidelines: ............................................................................................... 10
2.6 Harmonized regulations and standards ................................................................. 11
2.7 Marking of the listed harmonized standards and rules ........................................... 11
2.8 Revision ................................................................................................................ 11
3 Product description ........................................................................................ 12
3.1 Intended use: ........................................................................................................ 12
3.2 Reasonably foreseeable misuse: ........................................................................... 12
4 Safety instructions .......................................................................................... 14
4.1 General: ................................................................................................................ 14
4.2 Grounding ............................................................................................................. 15
4.3 Fuse requirements and current protection ............................................................. 15
4.4 Overvoltage protection .......................................................................................... 15
4.5 Laser Safety .......................................................................................................... 15
4.6 Leakage Current.................................................................................................... 17
4.7 Liquids ................................................................................................................... 17
Small Animal Imaging System
MSOT inSight64, inVision128 and inVision256-TF
v3.0, December 11, 2014
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MSOT inVision Series
User Manual – Part 1 – System Hardware
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4.8 Desinfection / Cleaning.......................................................................................... 17
4.9 Supply Voltage ...................................................................................................... 17
4.10 Ventilation requirements ................................................................................. 18
4.11 Requirements for special services, for example, air, cooling liquid .................. 18
4.12 Air filter mat ..................................................................................................... 18
4.13 Mechanical Hazard ......................................................................................... 19
4.14 Interlock .......................................................................................................... 19
4.15 Anesthesia Supply .......................................................................................... 19
4.16 Heating ........................................................................................................... 20
5 Technical data ................................................................................................. 21
5.1 Dimensions ........................................................................................................... 21
5.2 Power Supply ........................................................................................................ 21
5.3 Laser ..................................................................................................................... 21
5.4 Anesthesia Supply ................................................................................................. 21
5.5 Environmental Conditions ...................................................................................... 22
6 Transport and Application .............................................................................. 23
6.1 Transport ............................................................................................................... 23
6.2 Assembly............................................................................................................... 25
6.2.1 Assembly of parts .................................................................................. 25
6.2.2 Final assembly ...................................................................................... 25
6.3 Safety checking ..................................................................................................... 25
6.4 Commissioning ...................................................................................................... 26
6.4.1 Power Supply ........................................................................................ 26
6.4.2 USB und Ethernet Connections ............................................................. 26
6.4.3 Oxygen and anesthesia ......................................................................... 27
7 Equipment operation ...................................................................................... 29
7.1 Principle of Operation ............................................................................................ 29
7.2 Starting the Device ................................................................................................ 29
7.3 Placing the Animal ................................................................................................. 30
Small Animal Imaging System
MSOT inSight64, inVision128 and inVision256-TF
v3.0, December 11, 2014
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User Manual – Part 1 – System Hardware
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7.4 Performing Measurements .................................................................................... 33
7.5 Shutting down ....................................................................................................... 34
8 Service and Maintenance ............................................................................... 35
8.1 Contacts for Technical Support ............................................................................. 35
8.2 Change of Water ................................................................................................... 35
8.3 Changing the filters ............................................................................................... 37
8.4 Cleaning and Disinfection ...................................................................................... 38
8.4.1 Recommended disinfectants/detergents ............................................... 38
8.4.2 Cleaning and disinfection of the exterior of the device .......................... 39
8.4.3 Cleaning and disinfection of imaging chamber and internal tubing ........ 39
8.5 Notes on Replacing Components .......................................................................... 39
8.6 Preventative Cleaning and Maintenance Routines ................................................ 40
8.7 Spare Parts and consumables ............................................................................... 40
9 Waste of Electrical and Electronic Equipment (WEEE) ............................... 42
10 Trouble Shooting ....................................................................................... 43
11 Warranty ..................................................................................................... 44
12 EG Declaration of Conformity .................................................................. 45
Small Animal Imaging System
MSOT inSight64, inVision128 and inVision256-TF
v3.0, December 11, 2014
15.3.1-M-01
MSOT inVision Series
User Manual – Part 1 – System Hardware
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1 Warning Symbols
1.1 Legend of symbols:
1.1.1 Warning signs
Warning!
Warning! Risk of electric shock.
Caution, hot surfaces!
Warning! Hazardous laser radiation.
Caution, mechanical hazard.
Small Animal Imaging System
MSOT inSight64, inVision128 and inVision256-TF
v3.0, December 11, 2014
15.3.1-M-01
MSOT inVision Series
User Manual – Part 1 – System Hardware
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1.1.2 Signs advising of necessary activities
Read all safety and operating instructions
carefully before operating this equipment.
Unplug the device from main power supply
before opening.
1.1.3 Safety Labels
1.1.3.1 Location of labels
A
B
D
E C
Small Animal Imaging System
MSOT inSight64, inVision128 and inVision256-TF
v3.0, December 11, 2014
15.3.1-M-01
MSOT inVision Series
User Manual – Part 1 – System Hardware
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E
F
G
Small Animal Imaging System
MSOT inSight64, inVision128 and inVision256-TF
v3.0, December 11, 2014
15.3.1-M-01
MSOT inVision Series
User Manual – Part 1 – System Hardware
page 8 / 45
1.1.3.2 Label types
Label A
Label B
Label C
Label D
Label E
Label F
Label G
Small Animal Imaging System
MSOT inSight64, inVision128 and inVision256-TF
v3.0, December 11, 2014
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MSOT inVision Series
User Manual – Part 1 – System Hardware
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2 About this manual
2.1 User manual:
The user manual is intended for trained operation staff as well as qualified service and maintenance staff.
Before commissioning and first use the user manual has to be read in its entirety!
2.2 Declaration of Conformity:
Changes of animal scanner components result in expiration of the Declaration of
Conformity.
Therefore, changes are only allowed according to an agreement with the manufacturer.
The EG Declaration of Conformity is attached as the last page of this user manual.
2.3 Operation and expert staff:
The operation staff has to be introduced to the modes of operation of the animal scanner
before use. Of particular note is the identification of possible risks. The safety instructions
must therefore be noted.
The animal scanner is only allowed to be serviced by iThera Medical GmbH or its
distributors’ trained staff.
Maintenance is only allowed by qualified, permitted staff.
Personnel not previously qualified by iThera Medical
GmbH is not allowed to open any sealed enclosure.
2.4 Product liability:
iThera Medical is only responsible for personal injury or material damage which occurred
during intended use as described by iThera Medical and if all safety installations are fully
functional and the user manuals of the manufactures of the individual components as well
as all safety instructions have been observed.
Small Animal Imaging System
MSOT inSight64, inVision128 and inVision256-TF
v3.0, December 11, 2014
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User Manual – Part 1 – System Hardware
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iThera’s liability shall be limited to acts of willful misconduct or gross negligence by its
staff and limited to claims for the injury of life, body or health of a person. Any liability
pursuant to the Act on Product Liability (‘Produkthaftungsgesetz’) shall remain unaffected.
Notwithstanding the aforementioned, iThera shall not be liable for any incurred indirect or
consequential damages, including but not limited to lost profits, lost business or lost
revenue.
Unless, and then only to the extent that, the applicable law requires differently, the
aggregated liability from or in relation to this Agreement shall be limited to the contract
value.
The above limitation of liability shall also apply to the iThera’s employees, representatives
and vicarious agents. The customer has to immediately notify iThera in writing of any
incident of liability or expected liability under this Agreement to enable the concerted
mitigation of damages.
2.5 General guidelines:
The safety instructions in the user manual and the user manuals of the manufacturers of
the individual components give information to the user which ensures a safe und
functional working of the animal scanner.
If such details are neglected this could lead to errors,
damages or disturbances of the intended operating
procedure. Failure to comply with the safety instructions
could potentially lead to injury of employees in the
workplace.
Small Animal Imaging System
MSOT inSight64, inVision128 and inVision256-TF
v3.0, December 11, 2014
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User Manual – Part 1 – System Hardware
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2.6 Harmonized regulations and standards
Document Edition Title
2006/95/EC 2006-12-12 (Low voltage directive) Directive of the councilor for
approximation of laws of Member States which regards
electrical production equipment for use within certain
voltage limits
2004/108/EC 2004-12-15 (Electromagnetic compatibility) Directive of the councilor
for approximation of laws of Member States about the
electromagnetic compatibility
2.7 Marking of the listed harmonized standards and rules
EN 61010-1 2010 Safety requirements for electrical equipment for
measurement, control and laboratory use – Part : General
requirements
IEC 61326-1 2006 Electrical equipment for measurement, control and
laboratory use - EMC requirements - Part 1: General
requirements
IEC 60825-1 2007 Safety of laser products –
Part 1: Equipment classification and requirements
2.8 Revision
Revision 3, December 11, 2014
Editors: Josef Konradl, iThera Medical GmbH, Harry Reichert, SGS Germany GmbH
Small Animal Imaging System
MSOT inSight64, inVision128 and inVision256-TF
v3.0, December 11, 2014
15.3.1-M-01
MSOT inVision Series
User Manual – Part 1 – System Hardware
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3 Product description
3.1 Intended use:
The inSight / inVision series of preclinical small animal scanners are intended for
anatomical, functional and molecular imaging of mice and small rats.
Biological processes and the effect of pharmacological substances can be observed in
vivo and in real time, in high resolution, on a cellular and molecular level.
The product is allowed only to be used in a laboratory environment with restricted access
only. No public use is intended.
The use of the product is only allowed according to the description in this user manual.
All other use is not permitted.
3.2 Reasonably foreseeable misuse:
The device is not intended for clinical use
Do not put flammable materials into the imaging chamber
Place the imaging specimen only in accordance with the
instructions in Chapter 7.3 - Placing the Animal
To achieve quantitative results, the device has to be
calibrated with a phantom before performing
measurements
Use only deionized water for measurements
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MSOT inSight64, inVision128 and inVision256-TF
v3.0, December 11, 2014
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User Manual – Part 1 – System Hardware
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Use only with the delivered plug: Mennekes, 16A, 200-250
V, IP 44, TYP 148A
Caution – Use of controls or adjustments or performance of
procedures other than those specified in this user manual
may result in hazardous radiation exposure
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MSOT inSight64, inVision128 and inVision256-TF
v3.0, December 11, 2014
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User Manual – Part 1 – System Hardware
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4 Safety instructions
4.1 General:
The corresponding occupational safety regulation, the accident prevention regulation and
the user manuals of the manufacturers of individual components are valid for the
commissioning of the animal scanner.
The animal scanner is built according to the general accredited technical rules. It
conforms to the standards of LVD directive 2006/95/EG.
Risks for persons and material can come from the components of the animal scanner if
the safety instructions are ignored, if the animal scanner is unconventionally operated or
if the handling of the individual components is faulty.
Examples for unconventional handling, which can impair the safety of the device:
Disregard of warning signs and indication lights
Unauthorized maintenance
Read all safety and operating instructions carefully before
operating this equipment.
If the device is not operated in a way described in this
manual, safety may be impaired.
If it is assumed that a safe operation is no longer
possible, immediately inform the manufacturer,
especially in the case of visible damage or malfunction.
Opening any sealed enclosure of the animal scanner is
strictly forbidden by non-authorized personnel. Do not try
to repair the device by yourself. This could result in
equipment damage, malfunction, electric shock, fire or
serious injury.
Small Animal Imaging System
MSOT inSight64, inVision128 and inVision256-TF
v3.0, December 11, 2014
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User Manual – Part 1 – System Hardware
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The unit is equipped with safety interlocks to prevent the
emission of hazardous laser radiation. Manipulations can
lead to serious eye damage. Refrain from any attempt to
override or to shortcut the safety interlock.
4.2 Grounding
The unit must have adequate PE-connection to avoid
electric shock in case of a single fault.
4.3 Fuse requirements and current protection
A building fuse with rating 230V/16A is required.
There are no user replacable fuses inside the product.
4.4 Overvoltage protection
For overvoltage protection a blocktrap is used of type BT-1S-230AC/A, Phoenix Contact.
4.5 Laser Safety
The small animal, when put in place, receives laser radiation from two circular arranged
rows of five fiber optic arrays each. Both circles are placed axially apart. The fiber optic
arrays emit the laser radiation towards the centres of the circles onto the small animal.
The laser radiation is emitted in very short bursts of laser energy, 8 ns duration, 10 mJ
radiant energy per fiber optic array at a rate of ten bursts per second. The wavelength is
set by the user within the range from 680 nm (red, visible) to 980 nm (infrared, invisible).
From this data the radiant power can be calculated. It is 1.25 Megawatt. This very high
level of radiant power is needed to generate the ultrasound signal in the target. However,
it is also very dangerous to the eye for two reasons. One is the eye’s ability to produce
an image of the small fiberoptic laser source. Hence the eye focuses the light, which
penetrates the pupil, onto its retina. The other is the very short laser pulse with the
duration of nanoseconds to which the eye is very vulnerable.
Small Animal Imaging System
MSOT inSight64, inVision128 and inVision256-TF
v3.0, December 11, 2014
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User Manual – Part 1 – System Hardware
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Assuming the worst case, i.e. the access panel being kept open (interlocks defeated), a
fiberoptic array being viewed and the laser emitting, then the viewer’s eyes will almost
inevitably be injured. The accepted maximum permissible exposure (“MPE”), which is still
considered safe, is exceeded at least by two orders of magnitude (>100 x MPE).
Laser equipment is usually classified, from class 1 (safe) to class 4 (very dangerous),
according to the level of radiation which is accessible to humans. The accessible radiation
of this equipment, when the access panel is open and the laser is emitting, is that of laser
class 4. This would need a set of safety requirements being met by the user, including
the use of eye protecting laser safety glasses or -goggles. Since the access panel of this
equipment is safety-interlocked, there is no hazardous laser radiation accessible to
humans. The laser equipment is therefore classified and labelled as a laser class 1
although the laser source is class 4. Similar laser systems are called “embedded class 4
lasers”.
This means that the laser radiation safety of this equipment primarily depends on the
integrity of the safety interlocks and their wiring. The design of the safety interlocks is
redundant, i.e. the two interlocks cause the laser to stop emission via two separate
circuits.
The user should be aware of the fact that any attempt to defeat the interlocks would
put him/her and other nearby persons into a very hazardous situation.
The laser radiation is generated in a unit which comprises a frequency doubled flashlamp
pumped Nd:YAG laser (1064/532 nm). Its radiation is coupled into an OPO (optical
parametrical oscillator) which produces the desired wavelengths. The output is guided via
glass fiber bundles to the target. The level of radiation within this unit is dangerous. This
unit is separately housed, thus preventing leakage of hazardous laser radiation to the
space within the equipment housing, except at the apertures where the fiberoptic cables
are attached. Both, the laser unit and the fiberoptic cables are safely contained within the
protective housing of the equipment.
The user should be aware of the fact that any attempt to open the protective
housing would present a serious hazardous situation to him or her.
Maintenance procedures, as described in this user manual neither require defeating the
safety interlocks of the access panel, nor require the user to remove part of the protective
housing. Service procedures which would include adjustments within the laser unit
including the fiberoptics are prohibited to be performed on the user level, unless training
and certification is provided by the manufacturer.
Small Animal Imaging System
MSOT inSight64, inVision128 and inVision256-TF
v3.0, December 11, 2014
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User Manual – Part 1 – System Hardware
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4.6 Leakage Current
Warning! No extensive high leakage current expected if
device is suitably connected to supply mains.
4.7 Liquids
Please refer to chapter 8.
4.8 Desinfection / Cleaning
For details see chapter 8.4.
4.9 Supply Voltage
Please refer to chapter 6.4.1.
Small Animal Imaging System
MSOT inSight64, inVision128 and inVision256-TF
v3.0, December 11, 2014
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User Manual – Part 1 – System Hardware
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4.10 Ventilation requirements
Keep at least 50 cm space between wall and side vents. On the back and the top of the device no space for ventilation is required.
For stable operation the room temperature has to be in the range of 15°C to 25°C.
4.11 Requirements for special services, for example, air, cooling liquid
Once a year, the internal laser cooling system has to be maintained by an iThera service
technician.
4.12 Air filter mat
The air filter mat has to be checked yearly and if necessary replaced by an iThera service
technician.
min. 50 cm min. 50 cm
Small Animal Imaging System
MSOT inSight64, inVision128 and inVision256-TF
v3.0, December 11, 2014
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User Manual – Part 1 – System Hardware
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4.13 Mechanical Hazard
The positioning stages are calibrated while entering the acquisition mode. Do not put a
finger or other parts into the gap between the positioning stage and the imaging chamber.
4.14 Interlock
The access panel is equipped with safety interlocks to prevent the emission of hazardous
laser radiation. It is not allowed to defeat or override the safety interlocks.
For additional information see 4.5 (Laser Safety).
4.15 Anesthesia Supply
The device is equipped with internal tubing and connectors for anesthesia supply and
exhaust.
If oxygen is used parallel to anesthesia, the accumulation of excess or exhaled oxygen
together with the presence of any combustible material poses a severe risk of fire.
Therefore, it is important that the user takes care of appropriate precautions.
Only use anesthesia devices which are intended for small
animals and which are in accordance with the
specifications given in chapter 5.4.
Small Animal Imaging System
MSOT inSight64, inVision128 and inVision256-TF
v3.0, December 11, 2014
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User Manual – Part 1 – System Hardware
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For commissioning of the anesthesia system please refer to chapter 6.4.3.
4.16 Heating
A constant temperature of the water inside the imaging chamber is maintained through
heating mats on the outside of the chamber and temperature sensors (image).
Make sure that these sensors are immersed in water during operation.
Caution, metallic chamber surfaces can get hot!
Always use a suitable exhaust and absorption filters to
avoid any accumulation of anesthesia gas or oxygen
inside the device.
Make sure the room is sufficiently ventilated.
Small Animal Imaging System
MSOT inSight64, inVision128 and inVision256-TF
v3.0, December 11, 2014
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MSOT inVision Series
User Manual – Part 1 – System Hardware
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5 Technical data
5.1 Dimensions
Height (including wheels):................................................................. 1650 mm (65.0 in)
Height of Workbench (approx.): .......................................................... 915 mm (36.0 in)
Width (including hinges): .................................................................... 895 mm (35.2 in)
Depth: ............................................................................................... 1041 mm (41.0 in)
Weight (approx.): ................................................................................................ 500 kg
5.2 Power Supply
Input: .................................................................................. 230V, 50/60 Hz, 16 A max.
Fuses ..................................................................................... no user replacable fuses.
Appliance Class ................................................................................................. Class 1
Protection Rating ................................................................................................... IP20
Battery ........................................... 2 AA and 2 AAA batteries for mouse and keyboard
5.3 Laser
Laser Class .................................................... Class 1, embedded Class 4 laser device
Wavelength range: ............................................... 680nm – 980 nm, 532 nm, 1064 nm
Pulse frequency: .................................................................................................. 10 Hz
Pulse length: ........................................................................................................... 8 ns
Pump energy (@ 1064 nm): .................................................................... 800 mJ, max.
OPO energy (@ 740 nm) ......................................................................... 120 mJ, max.
5.4 Anesthesia Supply
Agents ........................................Oxygen / Air / N2O mixture with up to 5% Isoflourane
Operating Flow Range: .............................................................. up to 1 litre per minute
Small Animal Imaging System
MSOT inSight64, inVision128 and inVision256-TF
v3.0, December 11, 2014
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User Manual – Part 1 – System Hardware
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Operating Pressure Range: .......................................................................... up to 5kPa
5.5 Environmental Conditions
Usage: ............................................................................................ For indoor use only
Altitude:..................................................................................................... up to 2000 m
Storage temperature: ................................................................................... 5°C – 40°C
Operation temperature: ............................................................................. 15°C – 25°C
Relative humidity: .......................................................................................... max. 80%
Voltage fluctuations: ........................................................ up to 10% of nominal voltage
Small Animal Imaging System
MSOT inSight64, inVision128 and inVision256-TF
v3.0, December 11, 2014
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User Manual – Part 1 – System Hardware
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6 Transport and Application
6.1 Transport
Considering the dimensions and the weight the device can only be lifted and transported
with suitable load pick up instruments (e.g. with forklift truck or pallet jack).
For safe operation the forklift should be moved underneath the device from the back into
the space between the wheels.
Small Animal Imaging System
MSOT inSight64, inVision128 and inVision256-TF
v3.0, December 11, 2014
15.3.1-M-01
MSOT inVision Series
User Manual – Part 1 – System Hardware
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Make sure that the forks are long enough to support the
whole device.
Do not access with the forklift from left or right side
underneath the device, as this can result in severe
damage.
Unit components have to be secured from atmospheric
influence during transport.
Mobile and loose parts as well as external wires have to
be secured before transport.
For movement inside a building the device is equipped with wheels and handles.
All wheels have integrated brakes, which have to be loosened before transportation and
fixed afterwards.
Small Animal Imaging System
MSOT inSight64, inVision128 and inVision256-TF
v3.0, December 11, 2014
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User Manual – Part 1 – System Hardware
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The wheels are designed for plane floor inside buildings
Do not attempt to use stairs or steps while rolling the
scanner
6.2 Assembly
6.2.1 Assembly of parts
The animal scanner must be installed according to an installation plan on a level plane
with enough bearing strength of the floor.
Individual parts must be cleaned before assembly.
Where applicable, transport locks must be removed from animal scanner.
All loose or individual transported components must be reinstalled.
6.2.2 Final assembly
Assembly and first commissioning is only allowed to be done by trained expert staff of the
manufacturer or its distributors.
6.3 Safety checking
Check the animal scanner components through a visual check for mechanical
damages!
Check the cables and wiring for insulation damages!
Check the safety installations (e.g. interlock) for secure function!
Check the whole working area for cleanliness and accessibility!
If you detect a defect during the checking do not commission the animal
scanner! Let the expert staff repair the animal scanner!
Make sure that environmental conditions are met as described in chapter 5.4.
Small Animal Imaging System
MSOT inSight64, inVision128 and inVision256-TF
v3.0, December 11, 2014
15.3.1-M-01
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User Manual – Part 1 – System Hardware
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6.4 Commissioning
6.4.1 Power Supply
The main power supply connection is a plug of type IEC 60309. It is located on the right
side of the device. The main power connection plug is considered as a disconnection
device.
The animal scanner is designed for a mains voltage of 230V and a maximum current of
16A.
Access to the power plug must not be obstructed by
furniture or other objects in order to disconnect the device
from the mains in case of a hazard!
Only use the delivered power cord of type IEC 60309. Do
not use adapters or extensions. Suitable socket outlet must
be used.
6.4.2 USB und Ethernet Connections
Via the network RJ45 jack the device can be connected to a LAN network.
The USB ports on the front side allow optional connection to external backup media or a
variety of peripheral devices.
Small Animal Imaging System
MSOT inSight64, inVision128 and inVision256-TF
v3.0, December 11, 2014
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User Manual – Part 1 – System Hardware
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6.4.3 Oxygen and anesthesia
The device can be connected to an anesthesia device and gas exhaust unit via the
connections on the right side of the device.
A suitable connector is delivered with the device. Replacement parts can be ordered from
iThera Medical. The tubing should have an inner diameter of 3mm.
The length of the USB cable must not exceed 3 m.
Both, LAN and USB cable must be kept within the
building.
Small Animal Imaging System
MSOT inSight64, inVision128 and inVision256-TF
v3.0, December 11, 2014
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User Manual – Part 1 – System Hardware
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The recommended aneasthesia unit can be obtained separately from iThera Medical.
Small Animal Imaging System
MSOT inSight64, inVision128 and inVision256-TF
v3.0, December 11, 2014
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User Manual – Part 1 – System Hardware
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7 Equipment operation
7.1 Principle of Operation
The inSight and inVision series are multispectral optoacoustic tomography scanners
intended for preclinical imaging of mice and small rats. Based on the photoacoustic effect,
intrinsic absorbers such as haemoglobin or melanin or exogenously administered
molecular probes can be visualized in vivo and in real time, allowing the spatial and
temporal quantification of optoacoustic signals.
7.2 Starting the Device
Before starting, fill the internal water tank until the marking with deionized water according
to the instructions in chapter 8.2.
After the system is started, the application “ViewMSOT” will start automatically.
By pressing “LoadHW” (please see Software Manual) the hardware system will be
initialized, water will be pumped from the water tank to the imaging chamber and the
water will be heated to a constant temperature.
To switch the system on, turn the key switch on the front
panel in clockwise direction.
A green indicator shows the actual status of the system.
If the system does not start, please switch the system off
for 10s and try again.
Small Animal Imaging System
MSOT inSight64, inVision128 and inVision256-TF
v3.0, December 11, 2014
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User Manual – Part 1 – System Hardware
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7.3 Placing the Animal
1
Place the frame inside the
mounting station, and ensure that
the frame is clamped into the
base.
2
Pull the coupling membrane
carefully to the front of the
mounting station.
3
Place the animal holder into the
mounting station.
To prevent water from leaking into the animal holder during
acquisition, ensure the frame and the animal holder are dry
before putting them into place.
Drying can be carried out using absorbent paper.
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MSOT inSight64, inVision128 and inVision256-TF
v3.0, December 11, 2014
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User Manual – Part 1 – System Hardware
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4
Cut the foil by pushing down on
the lid.
5
Fix the foil with magnetic stripes.
6
Place sample or mouse inside
holder
If placing a live animal make sure
to connect anesthesia first (7)
Close the frame by raising it to the
holder. Ensure that the frame
clicks into place.
7
The anesthesia can be
disconnected by pressing the
metal parts on the sides of the
connector.
Small Animal Imaging System
MSOT inSight64, inVision128 and inVision256-TF
v3.0, December 11, 2014
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User Manual – Part 1 – System Hardware
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Once the holder has been placed into the imaging
chamber, the frame must not be opened and closed again
as this will compromise the water seal.
Once the frame has been opened, the user must
thoroughly dry the frame and holder and repeat mouse
placement starting from step 1.
Small Animal Imaging System
MSOT inSight64, inVision128 and inVision256-TF
v3.0, December 11, 2014
15.3.1-M-01
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User Manual – Part 1 – System Hardware
page 33 / 45
7.4 Performing Measurements
Ensuring first that the laser is not firing, open
the flap of the small imaging device and
place holder inside the device.
Connect anesthesia connector of the device
to the mouse holder and close the flap.
The MSOT measurement is controlled by software. For details please refer to the
software manual.
Small Animal Imaging System
MSOT inSight64, inVision128 and inVision256-TF
v3.0, December 11, 2014
15.3.1-M-01
MSOT inVision Series
User Manual – Part 1 – System Hardware
page 34 / 45
7.5 Shutting down
After the measurement has been performned, turn off the laser and remove the mouse
holder from the system.
Press the button “Unload HW” and wait until the status popup disappears.
By closing the software tool “ViewMSOT” the water is pumped out of the imaging
chamber.
After the software has closed, please shut down the computer.
Only switch off the system after the screen turns black by turning the key switch
counterclockwise.
Make sure that the anesthesia supply valve is closed.
Even if the system is switched off, the heating of the laser
will stay on to maintain a constant temperature of the
internal crystals.
Do not unplug the system!
Small Animal Imaging System
MSOT inSight64, inVision128 and inVision256-TF
v3.0, December 11, 2014
15.3.1-M-01
MSOT inVision Series
User Manual – Part 1 – System Hardware
page 35 / 45
8 Service and Maintenance
To ensure safe operation the equipment must be regularly serviced by the manufacturer
once a year.
A description of the service tasks is documented in the inSight/inVision series service
manual.
Opening the side panels of the animal scanner is strictly
forbidden for non-authorized personnel. Do not try to
repair the device by yourself. This could result in
equipment damage, malfunction, electric shock, fire or
serious injury.
Except the parts explicitely described in chapter 8 the
device contains no parts that can be serviced by the user.
Should a malfunction occur, immediately stop operation
and contact the manufacturer.
8.1 Contacts for Technical Support
iThera Medical GmbH
Zielstattstrasse 13
81379 Muenchen
Germany
Phone: +49 (0) 89 7007 449 99
Fax: +49 (0) 89 7007 449 90
E-mail: support@ithera-medical.com
8.2 Change of Water
In this device deionized water is used as a coupling medium. To ensure a smooth and
safe operation, this water must be changed daily.
Small Animal Imaging System
MSOT inSight64, inVision128 and inVision256-TF
v3.0, December 11, 2014
15.3.1-M-01
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User Manual – Part 1 – System Hardware
page 36 / 45
To disconnect the tubing on the red cap pull
the brass fitting downwards until it pops out
of place.
Open the second tubing (overflow) in the
same way.
Now take the water tank out of the device.
To exchange the water open the red cap.
Small Animal Imaging System
MSOT inSight64, inVision128 and inVision256-TF
v3.0, December 11, 2014
15.3.1-M-01
MSOT inVision Series
User Manual – Part 1 – System Hardware
page 37 / 45
Install the water tank in reverse order.
Make sure to connect both tubings after the water tank
has been exchanged.
8.3 Changing the filters
The device is equipped with an antibacterial microfilter. This should be changed at least
once a month.
Proceed as follows:
Empty imaging chamber
Unscrew filter housing (transparent part) by turning it counterclockwise – be careful
there might still be water inside. It is recommended to use a small bucket or tissue
to absorb water, which might drip into the pump compartement
Remove filter cartridge from filter housing, empty remaining water from filter
housing
Clean filter housing with disinfectant
Insert new filter cartridge inside filter housing and screw it to the black holder, make
sure that the O-Ring is in place
Pump water into imaging chamber, start stirring pump and check if filter housing is
not leaking
Remark: For easy opening of the filter housing a filter wrench can be used. This wrench
is delivered with the system.
Small Animal Imaging System
MSOT inSight64, inVision128 and inVision256-TF
v3.0, December 11, 2014
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User Manual – Part 1 – System Hardware
page 38 / 45
Make sure, that no water is spilled, as this will be
detected by the internal leakage detector, all pumps will
be shut down and the system will show an error.
In case water was spilled and the leakage
detector (picture on the left) was activated
please carefully dry it with tissue or a blow-
drier.
8.4 Cleaning and Disinfection
8.4.1 Recommended disinfectants/detergents
For disinfection or cleaning the system the following substances are recommended:
Isopropanol 70%
Pursept FD (Merz Hygiene GmbH, Frankfurt am Main)
Deionized water with or without mild detergents
Small Animal Imaging System
MSOT inSight64, inVision128 and inVision256-TF
v3.0, December 11, 2014
15.3.1-M-01
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User Manual – Part 1 – System Hardware
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8.4.2 Cleaning and disinfection of the exterior of the device
Use a cloth lightly damped with one of the recommended agents of chapter 8.4.1 to clean
the exterior surfaces of the device.
Make sure not to spill any liquid into the system.
8.4.3 Cleaning and disinfection of imaging chamber and internal tubing
Fill tank with one of the recommended agents of chapter 8.4.1.
For instructions regarding exchanging the water tank please refer to chapter 8.2.
Pump detergent/disinfectant into the imaging chamber
Let the solution circulate through the internal pump. Please observe the suggested
reaction time given by the manufacturer of the detergent/disinfectant
To achieve a better cleaning result repeatedly pump the cleaning agent into the
tank and into the chamber
After the reaction time pump the solution back into the tank, remove the tank from
the system and dispose its content.
Flush the water tank with clean deionized water.
To flush the imaging chamber insert water tank with clean deionized water into the
system, pump the water into the chamber and let it circulate for several minutes.
Afterwards pump it back into the water tank and dispose of the water
Repeat until there is no residue of the disinfecitant/detergent inside the imaging
chamber and water circulation system
It is recommended that the water filter is exchanged when the system is being disinfected.
8.5 Notes on Replacing Components
Only use original spare parts and consumables supplied
by iThera Medical GmbH. Otherwise a reliable and safe
operation can not be guaranteed.
Small Animal Imaging System
MSOT inSight64, inVision128 and inVision256-TF
v3.0, December 11, 2014
15.3.1-M-01
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User Manual – Part 1 – System Hardware
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8.6 Preventative Cleaning and Maintenance Routines
Task Ref. Maintenance
Interval
Performed by
Change of water, chamber 8.2 Daily User
Disinfection of chamber 8.4 As needed User
Change of water filter,
chamber 8.3 Monthly User
Change of laser flashlamp N/A 20 million shots iThera service
Change of deionizing
cartrigde, laser N/A Yearly iThera service
Change of water filter, laser N/A Yearly iThera service
Check (and if necessary
change) of filter mat N/A Yearly iThera service
Check (and if necessary
change) of interlocks N/A Yearly iThera service
Tasks which are marked with “iThera service” can only be
performed by an iThera service technician.
Do not attempt to carry out these tasks by yourself!
8.7 Spare Parts and consumables
Part number Description
ACC-AHM Animal Holder (Mouse - 120mm)
ACC-AHR Animal Holder (Rat - 180mm)
ACC-ANTH Anesthesia System
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MSOT inSight64, inVision128 and inVision256-TF
v3.0, December 11, 2014
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User Manual – Part 1 – System Hardware
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ACC-MOUNT Mounting Station
ACC-PHANT Measurement Phantom
CBL-CLAMP Paw Clamps (4 pcs)
CBL-CONE Nose Cone (10 pcs)
CBL-DCART Deionizing Cartridge (Laser)
CBL-FILTL Water Filter (Laser)
CBL-FILTT Water Filter (Imaging Tank)
CBL-LAMP Laser Flashlamp
CBL-MEM10 Coupling Membrane (10x30m) - thin (10µm)
CBL-MEM20 Coupling Membrane (300m) - medium (20µm)
Spare parts and consumables can be obtained from iThera Medical GmbH.
Small Animal Imaging System
MSOT inSight64, inVision128 and inVision256-TF
v3.0, December 11, 2014
15.3.1-M-01
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User Manual – Part 1 – System Hardware
page 42 / 45
9 Waste of Electrical and Electronic Equipment (WEEE)
A safe and environmentally friendly disposal of working materials, auxiliary materials and
exchange parts must be arranged.
Mechanical exchange parts should be reworked and recycled.
Electrical exchange parts should be reworked and recycled.
The corresponding norms, also intra-company regulations, for the disposal of working
materials, auxiliary materials and exchange parts must be noted. The regulations of the
recycling management law must be acknowledged.
Small Animal Imaging System
MSOT inSight64, inVision128 and inVision256-TF
v3.0, December 11, 2014
15.3.1-M-01
MSOT inVision Series
User Manual – Part 1 – System Hardware
page 43 / 45
10 Trouble Shooting
Issue Solution
System does not start, after
key switch was turned
1. Check if the system is connected to appropriate
mains supply
2. Switch off for at least 10s and try again
Critical Hardware Error 1. Water inside the lower compartment.
Leakage detector (green cylindrical part on the
right side) has to be dried.
Reload Hardware.
2. Imaging chamber overheated.
Fill water chamber and reload Hardware.
Critical Hardware error
persists after reloading
Hardware
Unplug system from mains, wait for approx. one
minute and replug the system.
Small Animal Imaging System
MSOT inSight64, inVision128 and inVision256-TF
v3.0, December 11, 2014
15.3.1-M-01
MSOT inVision Series
User Manual – Part 1 – System Hardware
page 44 / 45
11 Warranty
The Seller warrants that the Product sold hereunder conforms to the written description
of the Product and will function in accordance with the specifications set out therein. In
case of more than minor defects the Seller shall be obliged to remediate the defect
through its on-site support staff during the warranty period.
Only if the Seller, or its support staff, determines that the defect cannot be remediated,
the Seller shall be obliged to deliver a replacement product. The assertion of further
claims by the Purchaser is excluded.
The warranty period shall be twelve (12) months from the date of provision of the product.
Small Animal Imaging System
MSOT inSight64, inVision128 and inVision256-TF
v3.0, December 11, 2014
15.3.1-M-01
MSOT inVision Series
User Manual – Part 1 – System Hardware
page 45 / 45
12 EG Declaration of Conformity
iThera Medical GmbH
Ingolstaedter Landstraße 1 85764 Neuherberg / Muenchen
Germany
Phone: +49 (0) 89 3187-3973 Fax: +49 (0) 89 3187 4170
E-mail: info@ithera-medical.com
CE – Declaration of Conformity for
Small Animal Scanner
inSight64, inVision128, inVision256-TF
We, iThera Medical, hereby declare, that the above mentioned product is in accordance with the following EU Directives:
Low Voltage Directive (2006/95/EG)
Electromagnetic Compatibility (EMC) Directive (2004/108/EG)
and their applied harmonized Standards:
EN 61010-1:2010
EN 61326-1:2006
EN 60825-1:2008
This document was signed under responsibility of:
_______________
Date: signature:
Company stamp
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