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Wnt11 controls cell contact persistence by local accumulation of Frizzled 7 at the plasma membrane. Witzel et al. J Cell Biol . (2006). Vertebrate Noncanonical Wnt Signaling. PCP signaling play key roles in cell polarization and migration during gastrulation. - PowerPoint PPT Presentation
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Witzel et al. J Cell Biol. (2006)
Wnt11 controls cell contact persistence by local accumulation of Frizzled 7 at the plasma membrane
Vertebrate Noncanonical Wnt Signaling
Veeman et al. Dev Cell. (2003)
PCP signaling play key roles in cell polarization and migration during gastrulation.
Gastrulation movements in zebrafish
Solnica-Krezel L. Curr Opin Genet Dev. (2006)
Noncanonical Wnts and Fz regulates cell adhesion
Frizzled7 interact with protocadherin which mediate selective cell–cell adhesion.
Medina et al. EMBO J. (2004)
Ectopic expression of Xwnt-5A leads to decreased Ca 2+-dependent cell adhesion.
Torres et al. J Cell Biol. (1996)
Wnt11 controls tissue morphogenesis by modulating E-cadherin-mediated cell cohesion.
Ulrich et al. Dev. Cell. (2005)
Wnt11 induces local accumulation of Fz7 at the plasma membrane
5pg mRNA 10pg mRNA 20pg mRNA
Lyn : membrane-bound kinase - membrane marker
Wnt11 locally accumulates with Fz7 at the plasma membrane
Wnt11 producing cell
Wnt11 receiving cell
Wnt11-induced Fz7 accumulations form on contacting plasma membranes
Wnt11 induces the accumulation of Fz7 on both cells.
Fz7-YFP and Fz7-CFPaccumulation
Dsh is recruited to Wnt11-induced Fz7 accumulations
Wnt11 induces the accumulation of Fz7 together with the Dsh at the plasma membrane in regions of cell contact.
Wnt11-induced accumulations of Fz7 modulate cell contact persistence
Wnt11-induced Fz7 accumulations increase the persistence of cell contacts.
Wnt11-induced accumulations of Fz7 modulate cell contact persistence
What is Flamingo?
Usui et al. Cell. (1999) Veeman et al. Dev Cell. (2003)
Flamingo, a seven-pass transmembrane cadherin, regulates planar cell polarity under the control of Frizzled.
Wnt11 and Fmi control convergent extension movements during zebrafish gastrulation.
Formstone et al. Dev Biol. (2005)
Seifert et al. Nat Rev Genet. (2007)
Fmi2 colocalizes with Fz7 and Wnt11 at cell contacts and increases persistence
Fmi2 is able to induce accumulation of itself and Fz7 independently of Wnt11
Fmi2 colocalizes with Fz7 and Wnt11 at cell contacts and increases persistence
Fmi MO : Fmi morppholinos targeted against fmi1a, fmi1b, fmi2
Endogenous subcellular sites of Wnt11 and Fz7 activity marked by Dsh-YFP recruitment to the plasma membrane
Slb mutant : inactive C-terminal truncated wnt11
Endogenous subcellular sites of Wnt11 and Fz7 activity marked by Dsh-YFP recruitment to the plasma membrane
Fm464 : plasma membrane marker
Wnt11 persist at cell contacts and is required for contact persistence
Wnt11-YFP + GPI-anchored RFP
Wnt11 persist at cell contacts and is required for contact persistence
Summary
Wnt11
Dsh Dsh Dsh
Dsh
Enhance cell adhesion
Fz7 accumulation
Dsh accumulation
Fmi colocalizes with Fz7
PCP pathway
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