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Title: Nitroprusside Reaction Purpose: To identify the existence of cysteine
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NITROPRUSSIDE REACTION
Group’s Member:ATIKAH (F02111045)
DESY INDRA WAHYUNI (F02111033)
Sodium Nitroprusside• Nitroprusside is a complex anion that
contain a centered octahedral iron, surrounded by five cianide ligands which firmly bond and one ligand of linier nitric oxide.
• Sodium nitroprusside test is performed to test for the presence of proteins 'cysteine' in the test solution.
Cysteine is found in most high-protein foods, including:• Animal
sources: pork, poultry, eggs, dairy• Plant sources: red peppers,
garlic, onions,broccoli, brussels sprout, oats, granola, wheat germ, sprouted lentils.
Beside having the –COOH group, -NH2 group and R group, cysteine also has a free –SH group (sulfhydryl group ).If it reacted with sodium nitroprusside in an excess ammonia resulting a red-coloured complex.
Reaction: [Fe3+(CN)5NO]2- + NH3 + RSH ⟶ NH4+
+ [Fe2+ (CN)5NOSR]2-
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