Dr Yellapragada Lifesciences Corporate Profile

BRANCHES: CHENNAI (HO) / BANGALORE / HYDERABAD / PUNE

COMPANY PROFILE

CORPORATE PRESENTATION

Dr Yellapragada Subbarow

(1895-1948)

Because he lived you may be alive and well today; because he

lived you may live longer He Transformed Science; Changed

Lives

Divisions of Dr Yellapragada Lifesciences

• Bio-Fugue (Inoculating Knowledge)

Training in Lifesciences / Biological / Basic & Analytical Sciences.

• Sherwood-HR (Soar Beyond The Horizon)

Retained search firm & Refined Recruitment solutions (All verticals)

• Laasya Advertising ( Complete Outdoor Branding)

Complete Outdoor Solutions – Online Promotion - Branding

Biological / Bio Pharmaceutical Services

Clinical Research

Clinical Data management

Drug Discovery services

Waste management

Microbiological studies(Basic & Clinical)

Biochemical & Phytochemical studies

Bio-Technology studies

Molecular biology studies

Molecular genetic studies.

Cell biology studies.

Nano-biotechnology studies.

Biological / Bio Pharmaceutical Services

Bio-remediation.

Immunological studies

R-DNA Technology studies

Cell line studies/Animal tissue culture studies.

Invitro anti-cancer studies.

Plant tissue culture studies.

MYCO-Technology studies.

Anti-Oxidant studies.

Anti – Inflammatory studies (Invitro/In vivo).

Biological / Bio Pharmaceutical Services

Anti-Diabetic Studies (Invitro/In vivo).

Anti – Proliferative studies (Invitro/In vivo).

Custom Assay Development Services (CADS).

Biologics/Biosimilars/Bio Pharma CMC Support Assays.

Bio- Analytical Services (PK/TK/Immunogenicity/ Invitro Assays).

Final Product Characterization

Efficacy.

Toxicology

Antibody Production, Purification, Labeling, and Characterization.

Host – Cell Contamination.

Biological / Bio Pharmaceutical Services

Animal Model Development.

Stability Services.

Microbial Gene Analysis.

Invitro Laboratory Testing Services

Proximate analysis.

Pesticide analysis.

Heavy metal analysis.

Toxic element analysis.

Additive analysis.

Extraneous Material analysis.

Food Dyes analysis.

Animal Feed analysis.

Fatty acid analysis.

Invitro Laboratory Testing Services

Preservatives analysis.

Trace elements analysis.

Minerals analysis

Myco-Toxin analysis.

Hazardous sludge analysis.

BOD/COD analysis.

Emission analysis.

Water/Waste water/Air/Soil/Pharma/Cosmetic product analysis.

Invitro Laboratory Testing Services

Rancidity

Nutritional labeling.

Cereals/Pulses/Nuts/Oils/Fats/Milk/Egg/Fruit/Vegetables/Beverages/Sea-Food analysis/Poultry/Meat/dairy products analysis.

Specialized Instrumentation / Facilities

AFM

HR-TEM

SEM

FT-IR

UV-SPEC

NMR

XRD

GC-MS

Chromatography (HPLC, HPTLC, TLC, Column & Ion exchange).

Specialized Instrumentation / Facilities

Soxhlet Apparatus

Flow Cytometer

Confocal Microscopy

DNA Sequencer

FPLC

RT-PCR

ICPMS

Atomic Absorption Spectrophotometer

Chemidoc

Specialized Instrumentation / Facilities

Fluorescence Microscope

GC-MS

LC-MS/MS

Multi Mod Micro plate reader

Electrophoresis –Blotting – (Southern, Northern & Western).

Radiobiology

Ultra centrifuge

Tissue culture Facility

Fermentor

Nanodrop-Spetrophotometer

PRODUCTS

Nanoparticles (E.g. Silver, Gold, CDS, Zns etc Synthetic and Green chemistry)

(For academic and research purposes only.)

Custom Formulations

Research & Development Bio- Pharmaceuticals

API’s (Pipeline) • Anti-Diabetic Agents. • Anti – Oxidants. • Anti-Inflammatory

Agents. • Anti-Obesity Agents. • Anti-Proliferate Agents.

Research & Development Biologicals (pipeline)

Monoclonal Antibodies.

Therapeutic Proteins.

Oncology & Retroviral

Drugs

Vaccines

Pre-Clinicals

Test Batch Production

Process development

Potency Testing

Immunogenicity

Toxicology

Batch Release Test

Dose Stability

Pre-Clinicals

Efficacy

Endotoxins

Exotoxins

Outer- membrane

Toxins

Physchem studies

Pyrogens

Enzymes

Catalases

Oxidases

Peroxidases

Amylase

Lactase

Aspartase

Protease

Nitrilase

Fumerase

Aminoacyclase

Cellulase

Amino-Acids

L- Glutamic acid

L- Glutamine

L- Lysine

L-Threonine

L-Serine

L-Isoleucine

L-Leucine

L-Valine

Amino-Acids

L-Arginine

L-Histidine

L-Alanine

Aspartic acid

Proline

Cysteine

Methionine

Tryptophan

Phenylalanine

Tyrosine

Bio - Nanotechnology

Nano-Particles (Silver, Gold, Carbon,

ZNS etc.) Nano-powders Nanowires

Bio - Informatics

Tools, Software’s and Databases:

Sequence Analysis

Structure Analysis

Molecular Modeling

Molecular Docking

Molecular Dynamics and

Simulation

Insilco Drug Designing

Bio – Agricultural (Pipeline)

Bio-Composting

Molasses storage

Mushroom cultivation

Animal Feed

Clinical Research

• Acute Oral Toxicity

• Acute Dermal Toxicity

• Acute Inhalation Toxicity

• Acute Dermal Irritation / Corrosion

• Acute Eye Irritation / Corrosion

• Skin Sensitization

ACUTE

• Repeated Dose 14/28 Days Oral / Dermal

Toxicity Studies SUB ACUTE

• Repeated Dose 90 / 180 Days Toxicity Studies

• Carcinogenicity Studies

SUBCHRONIC &

CHRONIC

KPO Services

We offer “Quality Service” in IP and Market Analytic Sectors strengthening biological science, Pharma, business, law-IT sectors.

SWOT Analysis.

Trend Analysis.

Six Sigma.

IDEAL.

Ice-berg Studies.

KOL Assessment.

Process Optimization.

Study design & data management.

Licensing assessment.

Analysis & Interpretation.

Literature Review.

Patent, Non-Patent preparation.

LPO Services

IPR Services.

Research & Drafting Services.

Patent Drafting &

Filing Services.

Litigation Support.

CRO Services

We are committed to provide quality central laboratory services

for clinical trials, understanding fully that the reliability of

clinical laboratory data is crucial for the success of each trial.

We maintain the integrity and confidentiality of your research

which is critical to the well being of each study subject and

overall success of the trial. The lab provides services that meet

the required regulatory standards of the pharmaceutical industry.

PHARMACOKINETICS

ADME PK / In – vivo & In – Vitro

Efficacy

Physchem Studies

Batch Release Testing

In-Vitro

ADME (Absorption, Distribution, Metabolism, Excretion)

In vitro ADME studies help in optimization of

pharmacokinetic characteristics of a drug candidate identify

the metabolic pathways and provide valuable inputs for the

design of in vivo studies. We offer both standardized as

well as customized assays.

In-Vitro

• Permeability (Caco2, MDCK and PAMPA) • Aqueous Solubility • Metabolic stability and biotransformation • Test systems (microsomes, hepatocytes, S9, recombinant enzymes) • Species (rodent, guinea pig, rabbit, canine, non-human primate, human) • Protein binding (Equilibrium Dialysis and Ultrafiltration) • CYP Induction • CYP Isozyme Mapping • CYP Inhibition • Metabolite ID and profiling • Mass balance and drug disposition

Molecular Biology

DNA sequencing to DNA services have become routine tools in

drug discovery research, preclinical and clinical development. Both

basic and advanced DNA technologies are used on a daily basis in

most research laboratories. Dr Yellapragada Lifesciences Network

offers you a wide range of services have follows

Gene Regulation

• RNA interference, siRNA, shRNA,

• Micro RNA, miR RNA interference

• Immunoassays • Gene expression analysis

Nucleic Acid Analysis

• DNA isolation and purification • RNA isolation and purification • cDNA synthesis • DNA sequencing • Cloning • PCR analysis • Quantitative real time PCR

• DNA Library Creation • DNA Library Screening

Nucleic Acid Analysis

•Cloning of target genes into vectors • Gene Amplification and optimization (PCR) • Site directed mutagenesis • DNA Sequencing

• Microbial identification • Fragment analysis • DNA Sub-cloning • DNA and RNA Isolation • Northern blotting • Gene expression analysis by Real Time PCR

Proteomics

• Protein expression profiling (Array based) • SDS-PAGE • Immunoblotting

Immuno-Phenotyping

• Acute myeloid leukaemia characterization

• Acute lymphoblastic leukaemia characterization

• T-ALL diagnosis • B-ALL diagnosis

• Chronic lymph proliferative disorder diagnosis

• Hairy cell leukaemia • Multiple myeloma

Cell biology

Cell based assays

Cell-based assays are a vital early step in the testing of your

compounds and drug candidates for activity.

• Proprietary Cell-Based Assays

• High-throughput Fluorescence based assays

• Primary Cells and Proprietary Cell based assays

Cell Assays

• Angiogenesis • Apoptosis • Cell Migration • Cell Cycle analysis (Flow cytometer) • Cell Viability (MTT, SRB, CyQuant, 3H) • Inflammation and Cytokines assays • Reporter Gene assays (Luciferase) • Glucose Uptake

Cell and Diagnostic Applications

• Cell cycle analysis • Cell proliferation assays

• Apoptosis assays • Mitochondrial membrane potential analysis

• Oxidative burst assay • Reactive oxygen species analysis

• Autophagy assay • Immunofluorescence (confocal microscopy)

• Cell isolation and expansion • Cell immortalization • Cell sorting services

• Hematopoietic stem cell analysis

Pharmacology

In-Vivo

•We offer wide range of preclinical in vivo pharmacology efficacy studies in the

therapeutic areas of inflammation, pain, metabolic diseases etc. In-house bred and

imported animals would be used in the studies.

Inflammation and Pyrogen

• LPS induced sepsis model in mice.

• Adjuvant induced arthritis model in rats

• Collagen induced arthritis model in mice

• Carrageenan induced paw oedema model

• Rat air pouch model

Pharmacology

In-Vivo

Metabolic disorders

• Diabetic Neuropathy models

• STZ and nSTZ induced Diabetes modelslHFD + STZ induced Diabetes Models

• Genetically Modified mice (Lepr db/db mice)

Pain

• Formalin induced pain in rats

• Acetic acid induced writhing model in Swiss albino mice

• Evaluation of analgesic activity by Tail flick

• Apparatus and Eddy's hot plate methods.

Pharmacology

In-Vivo

Oncology

• Subcutaneous xenograft models

• Orthotopic tumor models

• Metastatic models

• Syngeneic models

• Hollow fibre assay

Infective

• LD50 determination (Systemic infection or Mouse protection model)

• Thigh infection model

• Lung infection model

Safety Pharmacology

CENTRAL NERVOUS SYSTEM

• Motor activity

• Behavior

• Body Temperature

• Sensory/ Motor reflexes

CARDIO VASCULAR SYSTEM

• Systolic & Diastolic BP

• Mean Arterial Pressure

• Heart Rate

Toxicology

Dr Yellapragada Lifesciences Drug discovery Network has facilities and expertise to

perform all aspects of health and environmental safety testing with animals. We

continue to develop new techniques in line with SOP based system with advances in

regulatory requirements and also constantly working towards 3R principle.

• GUIDELINES FOLLOWED

• OECD, OCSPP (OPPTS), EC, ICH, Schedule Y, ISO and custom design

Toxicology - In vivo

• Acute oral Toxicity – Up an d Down procedure (UDP)

• Acute oral Toxicity –Toxic Class method

• Acute Dermal Toxicity (Rat/Rabbit)

• Acute Dermal irritation in Rabbit

• Acute Eye irritation in Rabbit

• Skin sensitization in Guinea pig: Buehler test, Magnusson & Kligman

• Repeated Dose 28-Day Toxicity Study in Rodents

• 90-Day Oral Toxicity in Rodents

ROUTE OF ADMINISTRATION

Oral, Dermal, Intra dermal, Subcutaneous, Intravenous, Intra peritoneal and

Intramuscular

Geno-Toxicity Studies

The impact of any chemical on DNA is genotoxic ability of given test item. Effects

of chemicals on the genetic material form an integral part of research and regulatory

requirement.

• Ames Test

• Chromosomal aberration test

• In vivo Micronucleus test

Eco-Toxicology Studies

The impact of chemicals in Eco-system is regulatory requirement. The following

studies are conducted to draw the eco-toxic effect of given test item.

• Algae Growth Inhibition Test

• Acute Immobilization Test (Daphnia Sp.,)

• Acute Toxicity in Fish

• Earthworm, Acute Toxicity Test

• Acute Oral Toxicity Test – Japanese quail

Analytical Chemistry

Dr Yellapragada Lifesciences Drug discovery Network is equipped with advanced

instruments to cater the needs of chemists /researcher in the area of structural

elucidation, mass characterization, determination of true elements, separation science

etc. The following are the list of equipment's completely operational by experts in

analytical chemistry. In addition our chemists would offer on need basis, suggestion,

help and inputs for any of our client needs / problems etc.

High performance Liquid Chromatography (HPLC)

• HPLC Method Development (Analytical/Bio-analytical)

• Method Validation (Analytical / Bio-analytical)

• Qualitative analysis

• Quantitative analysis

• Routine sample analysis or Subject sample analysis

• Pharmacokinetics studies using HPLC

High performance Thin Layer Chromatography (HPTLC)

• HPTLC Method Development

• Method Validation

• Qualitative analysis (Profiling / finger printing)

• Quantitative analysis

• Stability Sample analysis

• Routine sample analysis or Subject sample analysis

Nuclear Magnetic Resonance Spectroscopy (NMR)

• 1H -Proton NMR • 13C NMR • Cosy • Nosy • DEBT 135 • HMQC • HSQC • HMBC • APT

Gas Chromatography coupled with Mass Spectrometry

(GCMS)

• GCMS Method Development (Analytical/Bio-analytical)

• Method Validation (Analytical/Bio-analytical)

• Qualitative analysis

• Quantitative analysis

• Routine sample analysis or Subject sample analysis

• Pharmacokinetics studies using GCMS

Inductive coupled plasma Mass Spectrometry (ICPMS)

ICPMS techniques are robust and suitable methods of trace metals analysis amenable

to a wide array of sample types. Metals, plastics, proteins, ceramics many others can

be successfully analyzed using ICPMS methods.

• Total sulphur and sulphur species in hydrocarbon fuels

• Organotin species in marine sediments and biota, consumer goods, and drinking

water

• Mercury species in fish, industrial discharges, and petroleum processing

• Arsenic species in marine algae, food products, and drinking water

Inductive coupled plasma Mass Spectrometry (ICPMS)

• Brominated and phosphorus-based flame retardants in consumer goods

• Phosphorus and sulphur in biological samples

• Protein- and peptide-bound metals

• Pesticides and herbicides

• Chemical warfare agents

• Volatile organo halides in air samples

Other Services

• Bio-Analytical

• On-going Projects

• Patent Works: New Molecule Identification, Drug Discovery-ADEMT TOX

Studies

• Medicinal / Phyto Chemistry Lab work Studies

• Structural Interpretation, X-ray Crystallography, Structure Identification, Docking

Studies

Value Added Services

As a part of value added services we offer solutions and services to

B.Tech, M.Tech, M.Sc., M.Phil, and PhD, in Lifesciences / Biological /

Basic & Analytical Sciences.

– Project guidance for Life sciences/ Biological/ Basic & Analytical

sciences.

– Wet lab/invitro facilities available.

– Career counseling.

– Protocol designing

Contact Us @

Vishnu Kiran.M || Head – Research & Marketing

Dr Yellapragada Lifesciences

No 8916, LIG -1, TNHB, Ayappakkam,

Ambattur, (OPP ICMR), Chennai - 600017 INDIA

Mob: +91.7299697960, +91.9962192013.

Email: dna.vishnu@gmail.com / dyl.vishnu@aol.com